Innovative Food Science & Emerging Technologies
·
2026
·
IF 6.9
Chenxiao Chen,Yiran Yang,Maha Alharbi,Ashwag Shami,Fahad Saad Alhodieb,Saleh A. Alsanie,Haiying Cui,Lin Lin
Chlorogenic acid-loaded coffee shell-derived carbon dot nanoparticles (CGA/CSCD NPs) with enhanced antibacterial photodynamic therapy (aPDT) activity were developed, exhibiting strong fluorescence (~400 nm), a graphite-like structure, and around 3 nm size. The nanoparticles achieved efficient ROS generation and bacterial enrichment, showing superior antibacterial efficacy and biocompatibility. Applied to Carassius auratus , Megalobrama amblycephala , and Dicentrarchus labrax under 4 °C storage, the CGA/CSCD NPs-aPDT treatment inhibited 99% of bacterial growth over 7 days, maintaining counts at 10 4 –10 5 CFU/g. The treatment significantly slowed pH rise, inhibited TVB-N accumulation, and reduced lipid oxidation by 2–3 times compared to controls. Furthermore, the nanoparticles preserved fish quality by minimizing moisture loss and maintaining color stability and texture (hardness and chewiness). These results demonstrate that CGA/CSCD NPs-aPDT provides a sustainable and effective strategy for delaying spoilage and extending the shelf life of aquatic products during cold storage.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Jingyi Chen,Ling Yang,Jincan Chen,Wenzhen Liu,Di Zhang,Peng Xu,Tao Dai,Le Shang,Yongshuai Yang,Shuzhi Tang,Yuxiang Zhang,Huajian Lin,Zhuo Chen,Mingdong Huang
Antimicrobial photodynamic therapy (aPDT) is a new therapeutical modality highly advocated recently to meet the challenge of drug resistant bacteria. In this study, we developed a composite material to combine silver nanoparticle, a commonly used disinfectant, with a potent photosensitizer for aPDT. Interestingly, we found the photosensitizer activated the silver nanoparticles into silver ions under light illumination. At the same time, silver nanoparticles enhanced the generation of ROS by the photosensitizer. Together, the two components activates each other, leading to remarkable antimicrobial efficacy against not only gram-positive and -negative bacteria, but also drug resistant bacteria (Methicillin Resistant S. aureus , MRSA). Such antimicrobial effect was further demonstrated on a bacterial infection model on mice. We showed that the chemical treatment, the high temperature or the pressure during the preparation did not affect the cellulosic structures of the fabric based on the characterization of optical, mechanical and thermal properties of the composite material. aPDT is typically a localized therapy, depending on the contact of bacteria to the photosensitizers, while the silver nanoparticles released the silver ions into the solution and can kill the bacteria thereof. Thus, our current study demonstrates the complementarity of aPDT and silver nanoparticles and mutual promotion of antibacterial effects to even drug-resistant bacteria.
研究领域:
生物医学工程
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药学
·
微生物学
·
材料科学
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高影响因子
Theranostics
·
2022
·
IF 11.6
Bingshuai Zhou,Xiaolin Sun,Biao Dong,Siyao Yu,Liang Cheng,Songtao Hu,Wei Liu,Lin Xu,Xue Bai,Lin Wang,Hongwei Song
Antibacterial photodynamic therapy (aPDT) has emerged as an attractive treatment option for efficient removal of pathogenic bacteria. However, aPDT in deep tissue will encounter difficulties such as limited light penetration depth, insufficient oxygen (O 2 ) supply and inability to eliminate inflammation introduced by bacteria, which hinders its clinical application. Herein, the near infrared (NIR) strategy of simultaneously generating O 2 and CO was developed for aPDT based antibacterial therapy and mitigation of deep infection inflammation. Methods: We prepared NIR-mediated multifunctional aPDT nanoplatform (POS-UCNPs/ICG) producing therapeutic gas of O 2 and CO. The CO, O 2 and ROS generation of the nanoplatform were characterized by dye probes, respectively. The antibacterial activity and anti-inflammation of POS-UCNPs/ICG were demonstrated in vitro and in vivo . In addition, the therapeutic effects in vivo were serially analyzed by immunofluorescence staining, Masson's staining, hematoxylin and eosin staining, colony formation units (CFU) and so on. Results: NIR-mediated multifunctional aPDT nanoplatform was realized by combining the up-conversion nanoparticles (UCNPs) and partially oxidized SnS 2 (POS) nanosheets (NSs) as well as indocyanine green (ICG). Using a single 808 nm light, aPDT can be achieved via ICG molecules, meanwhile, O 2 /CO can be generated by POS NSs through upconversion light excitation. During the aPDT process, O 2 can enhance aPDT, while CO can regulate inflammation through the PI3K/NF-κB pathway. Therefore, POS-UCNPs/ICG groups had a highest percentage of healing area up to 91.55±1.26% in mouse abscess model. Conclusion: Due to enhanced aPDT and anti-inflammatory collaborative therapy, the POS-UCNPs/ICG composites showed remarkably accelerated recovery in animal abscess models. Such NIR light responsive nanoplatform with optimized antibacterial capacity and immunomodulatory functions is promising for clinical therapeutics of bacteria-induced infections.
研究领域:
光疗
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纳米技术
·
细菌学
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炎症研究
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生物医学工程
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DOI
PubMed
高影响因子
ACS Sensors
·
2022
·
IF 9.618
Yue Xu,Haiyan Chen,Shufen Xu,Ji Liu,Yang Chen,Lijuan Gui,Hua Li,Ruixi Li,Zhenwei Yuan,Bowen Li
Several photosensitizers have recently been proposed as novel approaches against β-lactamase-producing drug-resistant bacteria. However, these reported photosensitizers are rarely used for accurate recognition of drug-resistant bacteria. To tackle this challenge, the structurally modified photosensitizer CySG-2 based on a lipophilic cationic heptamethine indocyanine near-infrared (NIR) dye (IR-780) and an important synthesis intermediate of cephalosporin antibiotic (GCLE) not only achieved the accurate recognition of TEM-1 methicillin-resistant Staphylococcus aureus (MRSA) successfully but also achieved antimicrobial photodynamic therapy (aPDT) in animal models infected by drug-resistant bacteria. Accurate enzyme recognition and efficient photodynamic therapy capabilities allow CySG-2 to achieve one stone with two birds. In addition, CySG-2 could also promote the eradication of internalized MRSA by facilitating the autophagy process, which is synergistic with its capacity of inducing reactive oxygen species generation under NIR laser irradiation for aPDT. Collectively, it is an effective multifunctional photosensitizer with the potential ability to guide the optimal use of different antibiotics and apply them in clinical treatment.
研究领域:
药学
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微生物学
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生物医学工程
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传染病学
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DOI
PubMed
高影响因子
Nanoscale
·
2020
·Vol. 12
·期 26
·13948-13957
·
IF 6.895
Wenzhen Liu,Yuxiang Zhang,Wenwu You,Jianqiang Su,Shaohua Yu,Tao Dai,Yunmei Huang,Xueyuan Chen,Xiaorong Song,Zhuo Chen
Extensively drug-resistant Acinetobacter baumannii (XDR-AB) has raised considerable concerns due to its mortal damage to humans and its high transmission rate of infections in hospitals. However, current antibiotics not only show poor anti-infection effects in vivo but also frequently cause high nephrotoxicity and neurotoxicity. Herein, we report a near-infrared (NIR) light-initiated antimicrobial photodynamic therapy (aPDT) to effectively treat in vivo XDR-AB infections based on photosensitizer (PS) loaded upconversion nanoparticles (UCNPs, LiYF4:Yb/Er). Such nanoagents feature robust NIR triggered UC luminescence and high-efficiency energy transfer from UCNPs to the loaded PS, thereby allowing NIR-triggered generation of reactive oxygen species (ROS) for destroying the bacterial cell membrane. This strategy permits a high antibacterial activity against XDR-AB, resulting in a decline of 4.72 log10 in viability at a dose of 50 μg mL−1 UCNPs-PVP-RB with 980 nm laser irradiation (1 W cm−2). More significantly, we can achieve excellent therapeutic efficacy against deep-tissue (about 5 mm) XDR-AB infections without causing any side effects in the murine model. In brief, such NIR-activated aPDT may open up new avenues for treating various deep-tissue intractable infections.
研究领域:
纳米技术
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光动力疗法
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抗菌剂
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生物医学工程
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DOI
PubMed
高影响因子
Nanoscale
·
2018
·Vol. 10
·期 33
·15485-15495
·
IF 7.233
Yuxiang Zhang,Ping Huang,Dong Wang,Jincan Chen,Wenzhen Liu,Ping Hu,Mingdong Huang,Xueyuan Chen,Zhuo Chen
An alarming worldwide increase in microbial resistance to traditional drugs and classical pharmacophores has spurred the search for new antimicrobial compounds. Antimicrobial photodynamic therapy (aPDT) has recently emerged as an effective modality for the selective destruction of bacteria and other pathogenic microorganisms. However, some of the factors, including the aggregation of the hydrophobic photosensitizer (PS) in aqueous media and the inefficient biodistribution of PS limit its expansion to clinical conditions. In addition, the photoactivation under visible-light irradiation limits the therapeutic effect of aPDT for deep-tissue infection. To overcome these limitations, a PS (β-carboxyphthalocyanine zinc, CPZ) delivery system with lanthanide-doped upconversion nanoparticles (UCNPs, LiYF4:Yb/Er) and polyvinylpyrrolidone (PVP) was prepared and its antimicrobial (antibacterial and antifungal) activities were investigated. Such a near-infrared (NIR) triggered UCNPs-CPZ-PVP system significantly reduced the aggregation of CPZ and presented a high anti-infectious activity against multi-drug resistant (MDR) bacteria (methicillin-resistant Staphylococcus aureus by 4.7 log10 and MDR Escherichia coli by 2.1 log10) post aPDT (at 50 μg mL−1 UCNPs-CPZ-PVP with 0.5 W cm−2 980 nm light). In particular, UCNPs-CPZ-PVP showed high antifungal efficacy against Candida albicans. In vivo aPDT experiments were further carried out using an MDR bacterial infection murine model in the presence of 5 mm thick tissue specimens, demonstrating the great potential of UCNPs-CPZ-PVP against infections in deep tissue. Altogether, we reveal an efficient NIR-triggered nano-photosensitizer with promising antifungal and antibacterial efficacy for clinical antimicrobial therapy.
研究领域:
药理学
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微生物学
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纳米技术
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治疗学
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2021
·
IF 5.268
Juan Zhao,Lixian Xu,Hao Zhang,Yuhong Zhuo,Yanan Weng,Shuang Li,Dinghua Yu
Recently, increased attention has been focused on antibacterial photodynamic therapy (APDT) to treat multidrug-resistant bacterial infection due to the antibiotic abuse. Methylene blue has been used as a kind of efficient and cheap commercial photosensitizer in APDT. However, due to high hydrophilicity, methylene blue is not able to be transcellular intaken and accumulated efficiently. To promote accumulation and APDT efficiency of methylene blue, lipopeptide surfactin-methylene blue complex has been prepared through electrostatic interaction. The complex under LED irradiation was found to effectively reduce 5.0 Log 10 CFU and 7.6 Log 10 CFU for P. aeruginosa and S. aureus , respectively. The bacterial reduction efficiency is slightly higher than free methylene blue. The photosensitizers accumulation and APDT targeting protein have been characterized by fluorescence spectroscopy, fluorescence microscopy and protein electrophoresis techniques. These results demonstrated that more surfactin-methylene blue complex could be accumulated more into the cell, and inactivate bacteria through destroying intracellular protein under LED illumination. In comparison, free methylene blue under light could inactivate bacteria through destroying membrane protein and lipid structures. These results would provide valuable insight for developing advanced clinical medicine and designing photo-drug for photodynamic therapy.
研究领域:
药学
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微生物学
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生物医学工程
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DOI
PubMed
高影响因子
INORGANIC CHEMISTRY
·
2023
·
IF 4.6
Bao-Xuan Xie,Hai-Shuang Wang,Hui-Qian Zheng,Jin Xu,Li Chen,Fang-Zhong Zhang,Yu-Lin Wang,Zu-Jin Lin,Rong-Guang Lin
Antibacterial photodynamic therapy (aPDT) is regarded as one of the most promising antibacterial therapies due to its nonresistance, noninvasion, and rapid sterilization. However, the development of antibacterial materials with high aPDT efficacy is still a long-standing challenge. Herein, we develop an effective antibacterial photodynamic composite UiO-66-(SH)2@TCPP@AgNPs by Ag encapsulation and 4,4′,4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) (TCPP) dopant. Through a mix-and-match strategy in the self-assembly process, 2,5-dimercaptoterephthalic acid containing –SH groups and TCPP were uniformly decorated into the UiO-66-type framework to form UiO-66-(SH)2@TCPP. After Ag(I) impregnation and in situ UV light reduction, Ag NPs were formed and encapsulated into UiO-66-(SH)2@TCPP to get UiO-66-(SH)2@TCPP@AgNPs. In the resulting composite, both Ag NPs and TCPP can effectively enhance the visible light absorption, largely boosting the generation efficiency of reactive oxygen species. Notably, the nanoscale size enables it to effectively contact and be endocytosed into bacteria. Consequently, UiO-66-(SH)2@TCPP@AgNPs show a very high aPDT efficacy against Gram-negative and Gram-positive bacteria as well as drug-resistant bacteria (MRSA). Furthermore, the Ag NPs were firmly anchored at the framework by the high density of –SH moieties, avoiding the cytotoxicity caused by the leakage of Ag NPs. By in vitro experiments, UiO-66-(SH)2@TCPP@AgNPs show a very high antibacterial activity and good biocompatibility as well as the potentiality to promote cell proliferation.
研究领域:
材料科学
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纳米技术
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生物医学工程
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微生物学
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药学
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DOI
PubMed
高影响因子
Small
·
2024
·
IF 13
Bingshuai Zhou,Biao Dong,Songtao Hu,Wei Liu,Liheng Sun,Lin Xu,Xue Bai,Lin Wang,Manlin Qi,Hongwei Song
Photothermal treatment (PTT) has emerged as a promising avenue for biofilm elimination, yet its potential drawbacks, such as local hyperpyrexia and bacterial heat resistance, have posed challenges. To address these concerns, an innovative nanoplatform (Au@mSiO 2 -arg/ICG) is devised that integrates phototherapeutic and gas therapeutic functionalities. This multifaceted nanoplatform is composed of mesoporous silica-coated Au nanorods (Au@mSiO 2 ), supplemented with l -arginine ( l -arg) and indocyanine green (ICG), and is engineered for mild temperature PTT aimed at biofilm eradication. Au@mSiO 2 -arg/ICG nanoparticles (NPs) show excellent antibacterial effects through the generation of nitric oxide (NO) gas, heat, and reactive oxygen species (ROS) under 808 nm light irradiation. The ROS generated by ICG initiates a cascade reaction with l -arg, ultimately yielding NO gas molecules. This localized release of NO not only effectively curbs the expression of heat shock proteins 70 mitigating bacterial thermoresistance, but also reduces extracellular polymeric substance allowing better penetration of the therapeutic agents. Furthermore, this nanoplatform achieves an outstanding biofilm elimination rate of over 99% in an abscess model under 808 nm light irradiation (0.8 W·cm −2 ), thereby establishing its potential as a dependable strategy for NO-enhanced mild PTT and antibacterial photodynamic therapy (aPDT) in clinical settings.
研究领域:
纳米技术
·
生物医学工程
·
药学
·
微生物学
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治疗学
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产品关联
DOI
PubMed
高影响因子
Small
·
2023
·
IF 13.3
Ke Cheng,Henggang Wang,Shan Sun,Mingyu Wu,Hongzhe Shen,Ke Chen,Zhiyuan Zhang,Si Li,Hengwei Lin
Antibacterial photodynamic therapy (aPDT) is a promising antibiotics-alternative strategy for bacterial infectious diseases, which features broad-spectrum antibacterial activity with a low risk of inducing bacterial resistance. However, clinical applications of aPDT are still hindered by the hydrophobicity-caused inadequate photodynamic activity of conventional photosensitizers and the hypoxic microenvironment of bacterial infections. To address these problems, herein, a promising strategy is developed to achieve specific chemiluminescence (CL) imaging and enhanced PDT of bacterial infections using hemin-modified carbon dots (H-CDs). The H-CDs can be facilely prepared and exhibit favorable water solubility, augmented photodynamic activity, and unique peroxidase-mimicking capacity. Compared with the free CDs, the photodynamic efficacy of H-CDs is significantly augmented due to the increased electron–hole separation efficiency. Moreover, the peroxidase catalytic performance of H-CDs enables not only infection identification via bacterial infection microenvironment-responsive CL imaging but also oxygen self-supplied aPDT with hypoxia-relief-enhanced bacteria inactivation effects. Finally, the enhanced aPDT efficiencies of H-CDs are validated in both in vivo abscess and infected wound models. This work may provide an effective antibacterial platform for the selective imaging-guided treatment of bacterial infections.
研究领域:
生物医学工程
·
纳米技术
·
药学
·
传染病
·
治疗诊断学
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DOI
PubMed
高影响因子
Nano Today
·
2022
·
IF 18.962
Manlin Qi,Xuan Ren,Wen Li,Yue Sun,Xiaolin Sun,Chunyan Li,Siyao Yu,Lin Xu,Yanmin Zhou,Shuyan Song,Biao Dong,Lin Wang
Periodontal disease is one of the most common oral infectious diseases and closely related to many systemic diseases which is in urgent need of novel and effective drugs. In particular, the problems of deep lesion location and synchronization of antibacterial and anti-inflammatory needs to be addressed. Herein, near infrared (NIR) light triggered nanoplatform with antibacterial and anti-inflammatory dual-functions by antibacterial photodynamic therapy (aPDT), photothermal therapy (PTT) and gas therapy was realized by loading S-nitrosothiols (SNO) and ICG into mesoporous silica-coated gold nanorods . Using a single 808 nm NIR light, the excellent antibacterial efficacy can be achieved not only by aPDT-induced bacteria killing but also by PTT-triggered biofilm dispersal. Furthermore, SNO molecules would also be thermally activated to release nitric oxide (NO) gas molecules at the site of periodontal disease, exerting inflammation inhibition by suppressing the assembly of pro-inflammatory factors and NLRP3 inflammasome. Additionally, such nanoplatform could validly combat pathogenic bacterial biofilm, and modulate the inflammatory immunity, leading to favorable therapeutics on animal models with periodontal diseases. Hence, this design provides an effective solution to solve the problems of deep tissue treatment along with simultaneously antibacterial/anti-inflammatory capabilities, which provides a bright prospect for clinical treatment of periodontal diseases, even extended to other refractory infections in deep sites.
研究领域:
牙科学
·
药学
·
生物医学工程
·
免疫学
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DOI
高影响因子
Nano Today
·
2022
·
IF 18.962
Ziyou Wang,Bingwen Du,Xiangyu Gao,Yiling Huang,Meng Li,Zhaohan Yu,Jiyao Li,Xiuyuan Shi,Yi Deng,Kunneng Liang
Invasive bacterial infection interferes the normal orchestrated course of healing cascades during cutaneous regeneration. Hypoxia in the deep-seated infection microenvironment (IME) considerably precludes photonic disinfection efficacy, especially antibacterial photodynamic therapy (aPDT). Herein we develop IME-unlocked oxygen-evolving bio-catalytic fabrics composed of electrospun poly(ε-caprolactone) scaffolds, MXene/SnS 2 bio-heterojunctions (MX/SnS bio-HJs), and lactate oxidase (LOx) for deep-seated bacteria-infected cutaneous regeneration. LOx can deplete lactic acid produced by bacteria at infected site and yield hydrogen peroxide (H 2 O 2 ). The MX/SnS bio-HJs not only catalyze H 2 O 2 to produce hydroxyl radicals (·OH) through Fenton-like reaction, but also generate oxygen (O 2 ) to fortify aPDT under Near-infrared (NIR) light irradiation, consequently, expeditious disinfection is achieved. Moreover, in vivo results reveal that the bio-catalytic fabrics exert benign cutaneous regeneration ability by slaughtering bacteria, promoting hemostasis and collagen deposition. This work provides a tactic to instill fabrics with an IME-unlocked catalytic sterilization capability through endogenous O 2 evolution for remedying infected wounds.
研究领域:
生物医学工程
·
材料科学
·
光生物学
·
伤口愈合
·
细菌感染
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DOI
高影响因子
危险品化学品经营许可证（带存储）