PROCESS BIOCHEMISTRY
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2022
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IF 4.885
Chengchen Cai,Ming Zhang,Haiming Chen,Wenxue Chen,Weijun Chen,Qiuping Zhong
Mango juice (MJ) has high nutritional value and decent flavor. However, its aroma could be easily changed by heat treatment during processing, which affects its acceptability and quality. In this study, Bacillus subtilis -HNU-B3 that could transfer carotenoid, was obtained from orchard soil to improve the aroma of fermented mango juice (FMJ). Compared with MJ, the organic acid content of FMJ by B. subtilis -HNU-B3 was significantly changed, and the contents of succinic acid and malic acid were increased to 3.68 mg/mL and 2.04 mg/mL, respectively. Moreover, volatile compounds identified by headspace solid-phase micro-extraction gas-phase mass spectrometry in FMJ were norisoprenoid and acetoin, accounting for 51.52 % of the total volatile substances. In addition, B. subtilis -HNU-B3 fermentation significantly increased the antioxidant capacity and total phenol contents of MJ, and the activity of carotenoid cleavage dioxygenase was 188 U/L. In conclusion, the aroma of FMJ could be improved by B. subtilis -HNU-B3 through increasing the content of norisoprenoid and acetoin. Our results provide theatrical support for Bacillus subtilis to improve the aroma quality of FMJ.
研究领域:
食品科学
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微生物学
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DOI
高影响因子
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
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2017
·Vol. 92
·期 9
·2477-2487
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IF 3.135
Niyomukiza Samuel,Teng Bao,Xian Zhang,Taowei Yang,Meijuan Xu,Xin Li,Irene Komera,Tuyishime Philibert,Zhiming Rao
BACKGROUND 2,3-Butanediol (2,3-BD) has a wide range of applications in chiral molecular synthesis, biofuel additives, and in food flavor additive manufacturing. Fermentation is a favorable method for 2,3-BD production. However, it requires much time and produces several NADH related byproducts which compete with 2,3-BD production. Bacillus subtilis has an excellent ability for 2,3-BD production by biocatalysis. However, its production is limited by low intracellular NADH and the reversible property of acetoin reductase (AR/2,3-BDH). The whole cell biocatalyst process with two different NADH regeneration systems was designed for efficient production of 2,3-BD in B. subtilis 168. RESULTS Formate dehydrogenase and glucose dehydrogenase for NADH regeneration were successfully co-expressed with acetoin reductase in B. subtilis 168. After optimization of biocatalyst bioconversion conditions, B. subtilis 168/pMA5- bdhA-Hpa II- fdh yielded 74.5 g L −1 of 2, 3-BD with 9.3 g L −1 h −1 productivity by fed batch and 115.4 g of 2,3-BD was achieved using same batch bacterium by three repeated batch bioconversions. On the other hand, 63.7 g L −1 of 2, 3-BD was produced with 7.92 g L −1 h −1 productivity by B. subtilis 168/pMA5- bdhA-Hpa II- gdh . To our knowledge, the volume productivity obtained here is the highest ever reported for biocatalysis. CONCLUSION A higher productivity of 2,3-BD from acetoin was achieved by whole cell biocatalysis with NADH regeneration systems in B. subtilis 168. This approach can be applied for NADH related bio-based chemicals production to improve titer, yield and productivity. © 2017 Society of Chemical Industry
研究领域:
生物化学
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微生物学
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代谢工程
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DOI
高影响因子
Metabolomics
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2019
·Vol. 15
·期 3
·1-12
·
IF 3.167
Yuan Honglun,Xu Yong,Chen Yaozhong,Zhan Yangyang,Wei Xuetuan,Li Lu,Wang Dong,He Penghui,Li Shengqing,Chen Shouwen
Introduction Acetoin serves as a high value-added platform with a broad range of applications, and can be effectively produced by Bacillus licheniformis . However, its toxicity to the producing strain hinders the higher acetoin production, and current knowledge about the acetoin resistance mechanisms of B. licheniformis is quite limited. Objectives To comprehensively investigate the metabolic changes in B. licheniformis under acetoin stress. Methods We used gas chromatography-mass spectrometry based untargeted metabolomics approach to measure the metabolic profiles of B. licheniformis under 20, 40 and 80 g/L acetoin stress. Transcriptional analysis was conducted to verify the metabolomics results. Results A total of 119 metabolites were identified in our experiment. The metabolic responses of B. licheniformis to acetoin stress were as follows: (i) pentose phosphate pathway and tricarboxylic acid (TCA) cycle were negatively affected by acetoin stress. In turn, glyoxylate cycle was activated to supply malic acid. (ii) Acetoin stress induced the accumulation of serine, valine, leucine and protective osmolytes (glycine and proline). (iii) Acetoin stress induced a higher saturated fatty acid ratio, which indicated a lower fluidity of cell membrane that could inhibit the entry of acetoin into cytoplasm. (iv) Synthesis of phosphatidylserine was enhanced, and phosphatidylethanolamine content was probably increased under acetoin stress. Conclusions This study revealed the metabolic perturbations of B. licheniformis to acetoin stress. In response to acetoin stress, glyoxylate cycle was activated, protective osmolytes were accumulated, saturated fatty acid ratio was elevated and synthesis of phosphatidylserine was enhanced in B. licheniformis .
研究领域:
微生物学
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代谢组学
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生物技术
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产品关联
DOI
PubMed
高影响因子
COMPUTERS IN BIOLOGY AND MEDICINE
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2023
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IF 7.7
Jinyi Qian,Yuzhou Wang,Xiner Liu,Zijian Hu,Nan Xu,Yuetong Wang,Tianqiong Shi,Chao Ye
Acetoin was widely used in food, medicine, and other industries, because of its unique fragrance. Bacillus amyloliquefaciens was recognized as a safe strain and a promising acetoin producer in fermentation. However, due to the complexity of its metabolic network, it had not been fully utilized. Therefore, a genome-scale metabolic network model ( i JYQ746) of B. amyloliquefaciens was constructed in this study, containing 746 genes, 1736 reactions, and 1611 metabolites. The results showed that Mg 2+ , Mn 2+ , and Fe 2+ have inhibitory effects on acetoin. When the stirring speed was 400 rpm, the maximum titer was 49.8 g L −1 . Minimization of metabolic adjustments (MOMA) was used to identify potential metabolic modification targets 2-oxoglutarate aminotransferase ( serC , EC 2.6.1.52) and glucose-6-phosphate isomerase ( pgi , EC 5.3.1.9). These targets could effectively accumulate acetoin by increasing pyruvate content, and the acetoin synthesis rate was increased by 610% and 10%, respectively. This provides a theoretical basis for metabolic engineering to reasonably transform B. amyloliquefaciens and produce acetoin.
研究领域:
生物技术
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代谢工程
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微生物学
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产品关联
DOI
PubMed
高影响因子
Scientific Reports
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2017
·Vol. 7
·期 1
·1-10
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IF 4.259
Jia Xiaojing,Liu Ying,Han Yejun
Acetoin (3-hydroxy-2-butanone) is an important bio-based platform chemical with wide applications. In vitro enzyme catalysed synthesis exhibits great feasibility in the production of chemicals with high purity. In the present work, a synthetic pathway involving a two-step continuous reaction was constructed in vitro for acetoin production from pyruvate at improved temperature. Thermostable candidates, acetolactate synthase (coAHASL1 and coAHASL2 from Caldicellulosiruptor owensensis OL) and α-acetolactate decarboxylase (bsALDC from Bacillus subtilis IPE5-4) were cloned, heterologously expressed, and characterized. All the enzymes showed maximum activities at 65–70 °C and pH of 6.5. Enzyme kinetics analysis showed that coAHASL1 had a higher activity but lower affinity against pyruvate than that of coAHASL2. In addition, the activities of coAHASL1 and bsALDC were promoted by Mn 2+ and NADPH. The cascade enzymatic reaction was optimized by using coAHASL1 and bsALDC based on their kinetic properties. Under optimal conditions, a maximum concentration of 3.36 ± 0.26 mM acetoin was produced from 10 mM pyruvate after reaction for 24 h at 65 °C. The productivity of acetoin was 0.14 mM h −1 , and the yield was 67.80% compared with the theoretical value. The results confirmed the feasibility of synthesis of acetoin from pyruvate with a cell-free enzyme catalysed system at improved temperature.
研究领域:
生物化学
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酶学
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生物技术
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2020
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IF 5.774
Yan Li,Jian-Ying Dai,Zhi-Long Xiu
The salting-out extraction (SOE) systems based on ionic liquids (ILs) have attracted extensive attention in the separation of bio-based products, in which imidazolium ILs were widely studied. However, the high cost and toxicity have hindered their further industrial application. Hydroxylammonium ILs have the characteristics of cheap raw material, simple synthesis process and low toxicity, but are rarely used in the SOE systems. In this work, five hydroxylammonium ILs (2 cations and 4 carboxylate anions) were synthesized and used in SOE of bio-chemicals. The phase forming abilities of ILs with K 3 PO 4 and H 2 O were affected by the hydrophilicity of anions and cations. With the increase of carbon chain length, phase forming abilities of ILs increased, while the temperature had little influence. The partition behaviors of acetoin, ILs and 2,3-butandiol, and the selectivity of acetoin to organic acids were investigated and compared at different concentrations of ethanolammonium butyrate (EOAB) and K 3 PO 4 . In a SOE system consisted of 6% EOAB-38% K 3 PO 4 (w/w), the recovery of acetoin, IL and 2,3-butandiol was 92.7%, 76.0% and 86.0%, respectively, and the selectivity of acetoin to lactic acid and acetic acid was 16.46 and 3.85, respectively. The ATR-IR spectra showed the hydrogen bonds formed between acetoin and O-H, N-H, –COO – of hydroxylammonium IL played an important role in the efficient extraction of acetoin from fermentation broths.
研究领域:
生物化工
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化学工程
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2026
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IF 8.3
Xudong Liang,Yuchen Duan,Xiangyu Li,Fujia Zhao,Yunwei Li,Yiming Jian,Lei Shi,Jiayu Li,Guo-Dong Li
Detection of 3-hydroxy-2-butanone (acetoin), a characteristic volatile metabolite of Listeria monocytogenes (LM) , represents a promising non-invasive strategy for monitoring contamination levels of foodborne pathogens in food. However, current sensing materials suffer from poor selectivity and insufficient sensitivity at trace concentrations. Herein, a series of Pd-loaded Cd 2 GeO 4 (Pd/CGO) chemiresistive gas sensors that achieves highly selective and sensitive acetoin detection was reported. Among them, the 0.5 wt% Pd/CGO-based sensor exhibits a high response, with an ultrafast response time and a detection limit as low as 10 ppb. The sensor also demonstrates excellent selectivity over common interfering gases, good reproducibility, and robust long-term stability. Mechanistic investigations combining O 2 -TPD, XPS, and DFT calculations reveal that Pd loading not only increases the proportion of chemisorbed oxygen species on the CGO surface, which enhances the overall sensing response, but also serves as new active sites. This work demonstrates that rational Pd modification combined with dual-site engineering offers a promising paradigm for designing high-performance metal oxide gas sensors for pathogen biomarker detection in food.
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DOI
高影响因子
Metabolic Engineering Communications
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2018
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IF 0
Xiaojing Jia,Robert M. Kelly,Yejun Han
( R )-acetoin is a four-carbon platform compound used as the precursor for synthesizing novel optically active materials. Ethylene glycol (EG) is a large-volume two-carbon commodity chemical used as the anti-freezing agent and building-block molecule for various polymers. Currently established microbial fermentation processes for converting monosaccharides to either ( R )-acetoin or EG are plagued by the formation of undesirable by-products. We show here that a cell-free bioreaction scheme can generate enantiomerically pure acetoin and EG as co-products from biomass-derived D -xylose. The seven-step, ATP-free system included in situ cofactor regeneration and recruited enzymes from Escherichia coli W3110, Bacillus subtilis shaijiu 32 and Caulobacter crescentus CB 2. Optimized in vitro biocatalytic conditions generated 3.2 mM ( R )-acetoin with stereoisomeric purity of 99.5% from 10 mM D -xylose at 30 °C and pH 7.5 after 24 h, with an initial ( R )-acetoin productivity of 1.0 mM/h. Concomitantly, EG was produced at 5.5 mM, with an initial productivity of 1.7 mM/h. This in vitro biocatalytic platform illustrates the potential for production of multiple value-added biomolecules from biomass-based sugars with no ATP requirement.
研究领域:
生物化学
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合成生物学
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代谢工程
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DOI
PubMed
RSC Advances
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2018
·Vol. 8
·期 53
·30512-30519
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IF 2.936
Jian-Xiu Li,Yan-Yan Huang,Xian-Rui Chen,Qi-Shi Du,Jian-Zong Meng,Neng-Zhong Xie,Ri-Bo Huang
Acetoin is an important platform chemical with a variety of applications in foods, cosmetics, chemical synthesis, and especially in the asymmetric synthesis of optically active pharmaceuticals. It is also a useful breath biomarker for early lung cancer diagnosis. In order to enhance production of optical (S)-acetoin and facilitate this building block for a series of chiral pharmaceuticals derivatives, we have developed a systematic approach using in situ-NADH regeneration systems and promising diacetyl reductase. Under optimal conditions, we have obtained 52.9 g L−1 of (S)-acetoin with an enantiomeric purity of 99.5% and a productivity of 6.2 g (L h)−1. The results reported in this study demonstrated that the production of (S)-acetoin could be effectively improved through the engineering of cofactor regeneration with promising diacetyl reductase. The systematic approach developed in this study could also be applied to synthesize other optically active α-hydroxy ketones, which may provide valuable benefits for the study of drug development.
研究领域:
生物催化
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药物化学
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代谢工程
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生物技术
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产品关联
DOI
PubMed
高影响因子
Energies
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2022
·Vol. 15
·期 12
·4242
·
IF 3.252
Xue-Yong Li,Jia-Ying Huang,Qiang Zhou,Yuan-Yuan Xu,Ponnandy Prabhu,Ye-Wang Zhang
Acetoin, a four-carbon hydroxyl-keto compound, is used in the food, pharmaceutical, and chemical industries. The cascade enzymatic production is considered a promising and efficient method to produce acetoin. However, the stability and compatibility of the enzymes under the same catalytic conditions are challenges that need to be resolved. In this work, alcohol dehydrogenase, acetaldehyde lyase, and NADH oxidase were selected to work at the same conditions to efficiently convert ethanol into acetoin. These three enzymes were immobilized on epoxy-modified magnetic nanomaterials to obtain highly stable biocatalysts. The stability and the immobilization conditions, including temperature, pH, enzyme–carrier ratio, and immobilization time, were optimized to obtain the immobilized enzymes with a high catalytic activity. The cascade reactions catalyzed by the immobilized enzymes yielded a high conversion of 90%, suggesting that the use of immobilized enzymes is a promising way to produce acetoin.Keywords:immobilization;NADH oxidase;acetaldehyde lyase;alcohol dehydrogenase;acetoin;cascade enzymatic reactions
研究领域:
生物技术
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酶学
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化学工程
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DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2026
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IF 3.9
Chong Zhang,Yu-Dong Shan,Yi-Ying Lin,Yu-Le Wang,Zheng Yang,Yong Liu,Song-Hai Wu,Xu Han
Diacetyl (2,3-butanedione) is an important ingredient used in the food and perfume industries, whose synthesis is largely constrained by environmental pollution and low selectivity. In this study, Fe-MIL-88B_NH2 has been successfully synthesized via the microwave-assisted solvothermal method, which exhibits high reactivity in acetoin oxidation, affording 81.3% conversion and 95.8% selectivity toward diacetyl under mild conditions in the presence of NHPI (N-hydroxyphthalimide). Raman and a series of characterizations reveal that O2 is activated by surface Fe(II) on Fe-MIL-88B_NH2 with the generation of ≡Fe-OO• superoxo, which then abstracts H from acetoin with the formation of diacetyl and H2O2. Quenching experiments indicate that ≡Fe-OO• superoxo on Fe-MIL-88B_NH2 is the primary oxidizing species in the oxidation of acetoin. EPR and DPD analyses further reveal that the NHPI/PINO cycle effectively shuttles H from the acetoin to ≡Fe-OO• superoxo, thus enhancing the oxidation efficiency of acetoin. This study enriches our understanding on the importance of surface superoxo species and the H-shuttle in the selective oxidation of alcohols toward ketones.
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DOI
高影响因子
AQUACULTURE RESEARCH
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2023
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IF 2
Chunjing Cai,Zhe Li,Junhua Lu,Ying Wang,Chen Wang,Pengfei Li,Hui Wang,Wu Meng,Jie Chu
The purpose of this study was to investigate the effects of dietary acetoin on Largemouth Bass (Micropterus salmoides). In 45 d of farming, the experimental diets were supplemented with 0%, 0.3%, 0.6%, and 0.9% of acetoin levels, respectively. Results showed that the activities of lipase (LPS), amylase (AMS), and trypsin in fish fed with acetoin at 0.6% displayed the most obvious improvement compared with the other levels of acetoin (). Furthermore, the incorporation of acetoin at 0.3% and 0.6% in Micropterus salmoides diet significantly enhanced the number of beneficial bacteria (such as Cetobacterium) and reduced the number of harmful bacteria (such as Proteobacteria) in the gut flora of Micropterus salmoides. Meanwhile, the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and catalase (CAT) in the liver of the samples treated with acetoin at 0.6% were significantly higher than the control (), while the content of malondialdehyde (MDA) and the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the liver notably decreased with the increase of the addition amount of acetoin (). Furthermore, the contents of IgA, IL-6, and TNF-α and the activity of acid phosphatase (ACP) in the serum of the samples added with acetoin exhibited a concentration-dependent decline compared with the control (). In summary, acetoin had potential feeding attraction effects on Micropterus salmoides, and the most suitable dose of dietary application was 0.6% acetoin, which can effectively improve the physiological characteristics and immune activity of Micropterus salmoides.
研究领域:
水产养殖学
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动物营养学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）