FOOD RESEARCH INTERNATIONAL
·
2021
·
IF 6.475
Wei Jia,Zibian Fan,An Du,Lin Shi
Ageing is a time-consuming step in Baijiu manufacture, stimulating an urgent requirement of optimization. Variation of artificial aged Feng-flavor Baijiu by inhomogeneous alternating magnetic field was investigated through quantitative foodomics combined with confirmed ultra high performance liquid chromatography quadrupole-orbitaltrap high resolution mass spectrometry (UHPLC-Q-Orbitrap). A total of 153 substances were identified with significant variables (p < 0.05, VIP > 1) and 16 metabolic pathways related to Feng-flavor Baijiu functions were obtained. The method showed good accuracy with recovery values between 80.4% and 117.4% and precision lower than 9.8% for all characteristic substances. Limit of detection (LOD) was ranging between 1.6 and 10.0 μg/L with R 2 ≥ 0.99. Factor analysis demonstrated that ageing degree of magnetized samples increased with rise of magnetic field intensity and the maximum effect was equivalent to 12.81 years of natural ageing. The results of stoichiometric analysis revealed that regulation of magnetic field on proportion in Baijiu was mainly performed through entropy and the hydrogen bond strength of Baijiu molecules. Sensory evaluation illustrated that score of Baijiu samples reached the highest at 150 mT, demonstrating that magnetic field treatment can be considered as an optimized ageing means for Feng-flavor Baijiu.
研究领域:
食品科学
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分子生物学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Wei Jia,Zibian Fan,An Du,Lin Shi,Jinmei Ren
Honey aroma is a typical sensory characteristic of Feng-flavour Baijiu, which originates from a unique manufacturing process, the formation mechanism of which is unclear. Multivariate analysis combined with foodomics assisted by sensory evaluation was performed to investigate the molecular mechanism of honey aroma formation in Feng-flavour Baijiu during the 17-year ageing process. A total of 1995 compounds was identified, and 47 variables were screened as significant substances according to variable importance in projection and Spearman’s rank correlation coefficient (|ρ| > 0.7), which corroborated that the long-term interaction between Baijiu and storage containers was the dominant origin of honey aroma. Recombination and omission experiments further validated the important contributions of significant substances, including acids, alcohols, aldehydes and ketones. A typical honey aroma dominated by fruity, floral, sweet and nutty notes was successfully simulated, and nutty notes could be enhanced by amides, whereas amines presented masking effects on fruity and floral aromas.
研究领域:
食品科学
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化学
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DOI
PubMed
高影响因子
Aging-US
·
2022
·Vol. 14
·期 16
·6829
·
IF 5.955
Tomas Schmauck-Medina,Adrian Molière,Sofie Lautrup,Jianying Zhang,Stefan Chlopicki,Helena Borland Madsen,Shuqin Cao,Casper Soendenbroe,Els Mansell,Mark Bitsch Vestergaard,Zhiquan Li,Yosef Shiloh,Patricia L Opresko,Jean-Marc Egly,Thomas Kirkwood,Eric Verdin,Vilhelm A Bohr,Lynne S Cox,Tinna Stevnsner,Lene Juel Rasmussen,Evandro F Fang
Genomic instability, telomere attrition, epigenetic alterations, mitochondrial dysfunction, loss of proteostasis, deregulated nutrient-sensing, cellular senescence, stem cell exhaustion, and altered intercellular communication were the original nine hallmarks of ageing proposed by López-Otín and colleagues in 2013. The proposal of these hallmarks of ageing has been instrumental in guiding and pushing forward research on the biology of ageing. In the nearly past 10 years, our in-depth exploration on ageing research has enabled us to formulate new hallmarks of ageing which are compromised autophagy, microbiome disturbance, altered mechanical properties, splicing dysregulation, and inflammation, among other emerging ones. Amalgamation of the ‘old’ and ‘new’ hallmarks of ageing may provide a more comprehensive explanation of ageing and age-related diseases, shedding light on interventional and therapeutic studies to achieve healthy, happy, and productive lives in the elderly.
研究领域:
衰老生物学
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分子老年学
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细胞衰老
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系统衰老生物学
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转化老年科学
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DOI
PubMed
高影响因子
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
·
2025
·Vol. 57
·期 8
·1234
·
IF 3.4
Huihui Ju,Yile Zhou,Wanting Wei,Yan Hu,Hongwei Fang,Zhouyi Chen,Xia Sun,Yi Shi,Hao Fang
Ageing is an independent factor for cognitive dysfunction. Ageing-associated alterations in the gut microbiota also affect cognition. The present study is designed to investigate changes in the gut microbiota and their participation in ageing-associated cognitive impairment. Both 10-week-old and 18-month-old mice are used. Mouse cognition is examined by novel object recognition and T-maze tests. Mouse feces are collected for sequencing and transplantation. Protein expression in the mouse intestine and hippocampus is studied using immunohistochemistry and immunofluorescence staining. Senescent neurons are induced by hydrogen peroxide in vitro . The cell lysates are used for western blot analysis and adenosine triphosphate (ATP) measurement. Our results show that 18-month-old mice exhibit cognitive dysfunction compared with young mice. In aged mice, transplanting the microbiota of young mice increases the protein presence of synaptophysin in the hippocampus and partially restores cognition. The protein expressions of mucin-2 and E-cadherin in the intestine are reduced in aged mice but are increased by transplantation. Gut microbiota analyses reveal that the reduced abundance of the microbe Bacilli-Lactobacillales-Lactobacillaceae-Lactobacillus in aged mice is restored by transplantation. Fecal microbiota transplantation in young mice increases the serum level of acetic acid in aged mice. Hydrogen peroxide stimulation induces senescence and reduces the protein expression levels of synaptophysin and acetyl-coenzyme A synthetase member 2 (ACSS2) in primary neurons. Incubation with acetic acid upregulates the protein expressions of ACSS2 and synaptophysin and further increases ATP production in senescent neurons. In summary, gut microbiota transplantation increases the abundance of Lactobacillales , elevates serum acetic acid level, and improves cognitive function in aged mice. Gut microbiota transplantation has therapeutic importance for ageing-associated cognitive decline.
研究领域:
神经科学
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微生物学
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衰老研究
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胃肠病学
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认知科学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
PHYTOTHERAPY RESEARCH
·
2021
·Vol. 35
·期 10
·5767-5780
·
IF 5.882
Lin Zhu,Zhenchao Liu,Yuqian Ren,Xiaolin Wu,Yingjuan Liu,Tingting Wang,Yizhao Li,Yusheng Cong,Yunliang Guo
Studies have found that salidroside, isolated from Rhodiola rosea L , has various pharmacological activities, but there have been no studies on the effects of salidroside on brain hippocampal senescence. The purpose of this study was to investigate the mechanistic role of salidroside in hippocampal neuron senescence and injury. In this study, long-term cultured primary rat hippocampal neurons and naturally aged C57 mice were treated with salidroside. The results showed that salidroside increased the viability and MAP2 expression, reduced β-galactosidase (β-gal) levels of rat primary hippocampal neurons. Salidroside also improved cognition dysfunction in ageing mice and alleviated neuronal degeneration in the ageing mice CA1 region. Moreover, salidroside decreased the levels of oxidative stress and p21, p16 protein expressions of hippocampal neurons and ageing mice. Salidroside promoted telomerase reverse transcriptase (TERT) protein expression via the phosphatidylinositol-3-kinase (PI3K)/protein kinase B (Akt) pathway. In conclusion, our findings suggest that salidroside has the potential to be used as a therapeutic strategy for anti-ageing and ageing-related disease treatment.
研究领域:
神经科学
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药理学
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衰老研究
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分子生物学
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DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Hailong Ru,Haiyue Xu,Qu Li,Yougui Yu,Qing Zheng
Different Baijiu varieties are characterised by their specific flavours and ester aromas. These flavours originate from the process of esterification during long-term ageing. Despite their wide popularity and long history, the mechanism of esterification during ageing has not been experimentally demonstrated. This study aimed to elucidate the esterification mechanism experimentally to promote advances in the ageing technology for Baijiu and to help advance the Baijiu brewing industry to transform it into a carbon-neutral industry. In this study, traditional acid-catalysed conditions were applied and esterification during ageing was accelerated. Equilibrium constants were obtained for the major esters via thermodynamic calculations and these constants were corrected using the experimental results. These corrected constants can predict the acid and ester contents of the liquor during ageing. Thus, the results can also help standardise Baijiu brewing technology.
研究领域:
食品科学
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化学工程
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产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Xinxin Zhao,Chengcheng Ding,Shuguang Wang,Chao Song
Ageing is the inevitable fate of carbon nanotubes (CNTs) in natural environments. However, the ageing-induced changes in the mobility of CNTs are still poorly understood. In this study, we investigated the migration of CNTs with different ageing degrees in saturated quartz sand using ultrapure water, fresh water and seawater as background solutions. Ageing could change the physicochemical properties of CNTs, leading to less hydrophobicity, lower hydrodynamic diameter, more negative charge and higher dispersity. As a result, aged CNTs exhibited a higher migration ability than pristine CNTs. Furthermore, DLVO theory and XDLVO theory were applied to explore the interaction between CNTs and between CNTs and quartz sand, respectively. Electrostatic repulsion is the primary force, and the total force is also repulsion and increases with ageing, which is favorable for CNTs to migrate in quartz sand. In fresh water and seawater, high ionic strength reduced the electrostatic repulsion, as well as hydrodynamic diameters and negative charges, and thus ageing exhibited a weaker enhancement on CNTs migration than that in ultrapure water. This work is useful to better understand the migration of aged CNTs and is of great significance to reveal the fate of carbon nanomaterials in nature.
研究领域:
环境科学
·
纳米材料科学
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产品关联
DOI
高影响因子
IET Nanodielectrics
·
2026
·Vol. 9
·期 1
·e70035
·
IF 4.3
Wu Fa-Yuan,Li Xiang-qing,Luo Yi-Chen,Chen Shuo,Liu He-Chen
This study investigates the influence of different transesterification catalysts on the performance evolution of degradable epoxy resins under thermal-oxidative ageing. Three resin systems were prepared using triethanolamine (TEOA), 1,5,7-triazabicyclo[4.4.0]dec-5-ene (TBD), and zinc acetylacetonate as catalysts, followed by ageing tests at 220°C. The effects of ageing time and catalyst type on the electrical, thermal, mechanical properties and degradation behaviour of the resins were systematically analysed. Results reveal that prolonged ageing causes certain performance degradation, yet the resins still maintain favourable overall properties at the late ageing stage. The V-TEOA system shows the best performance retention after 42 days of ageing, with key indicators as follows: breakdown strength 36.7 kV/mm, dielectric loss factor 0.46%, leakage current 65.34 μA, flexural strength 67.32 MPa and temperature index 170.99°C. Meanwhile, thermal-oxidative ageing markedly weakens the degradation efficiency of the resins, and a distinct core–shell structure is found in the degraded V-TBD system. This work deepens the understanding of ageing behaviours of such resins and supports their practical application in the engineering.
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DOI
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2024
·
IF 4
Xiaojing Zhang,Ying Liu,Xin Zhang,Huiping Liu,Bing Wang,Can Li,Huihui Zhang,Xiaoxiao Ma,Xiaowei Zhang
In this investigation, we have effectively extracted and characterized a new Exocarpium Citrulli polysaccharide (ECP-2) with a molecular weight of 1.67 × 10 6 Da using hot water extraction and ethanol precipitation techniques. Analysis was performed using high performance gel permeation chromatography, ion chromatography, Fourier transform infrared spectroscopy, nuclear magnetic resonance, and scanning electron microscopy. ECP-2 is primarily composed of galactose (32.32 %), arabinose (25.42 %), and mannose (19.75 %). Fuc, Rha, Ara, Gal, Glc, Man, and GalA molar ratios in ECP-2 is 4.65:1.50:25.42:32.32:5.78:19.75:10.57. Based on structural studies, the primary sugar residues of ECP-2 are T-α-L-Ara f -(1→, →5)-α-L-Ara f -(1→, T-α-D-Man p -(1→ and →3,6)-β-D-Gal p -(1→. Furthermore, the anti-aging effect of ECP-2 in D-galactose model mice, while the results indicate that ECP-2 significantly enhances the activities of superoxide dismutase, glutathione peroxidase, and catalase in D-galactose model control mice. Additionally, ECP-2 effectively eliminated the lipid peroxidation product malondialdehyde, thereby restoring normal growth of the mice by regulating their defense mechanism. In conclusion, the polysaccharides of Exocarpium Citrulli , particularly ECP-2, may be appropriate for use in natural medicines and functional foods to halt or slow the aging process.
研究领域:
药理学
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生物化学
·
分子生物学
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食品科学
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DOI
高影响因子
Nature Communications
·
2026
·Vol. 17
·期 1
·4775
·
IF 18.1
Peng Qiuming,Zhou Lutong,Wang Jinming,Tong Ke,Hu Wentao,Wang Lin,Sun Yong,Gao Yipeng,Nie Anmin,Yang Biaobiao,Cai Wei,Zou Guodong,Huang Tianlin,Zhu Wanquan,Tian Yongjun
Nanoscale twin boundaries (TBs) effectively restrict the free motion of dislocations by intersecting with other TBs to realize high strength. However, the dislocations parallel to the TBs can glide along the TBs, resulting in detwinning for low strength. Herein, we present a triple-interlocked-nanotwinned (TIT) strategy for overcoming these inherent deficiencies. An Mg-9Li alloy was used as a proof-of-concept. The treated alloy had a large volume fraction of TIT interfaces (65.7%). It not only had a high yield strength of 508.6 MPa (approximately eleven times higher than that of the TIT-free sample) but also offered unprecedented ageing resistance, with a reduction in hardness of 6% after 730 days. The martensite-like phase transformation process concurrently triggers multiple-variant twins to form TIT structures. These TIT nets can effectively prohibit dislocation motion, reduce strengthening anisotropy, and disperse localized stress during the deformation process. This strategy of phase transformation twinning open a gate to fabricate other stronger metallic or ceramic nanotwinned bulk materials.
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DOI
PubMed
高影响因子
Nature Nanotechnology
·
2025
·
IF 34.9
Liang Kaiyu,Zhao Lan,Zhang Shuheng,Zheng Luyu,Zhang Zheyuan,Wang Shengyu,Chen Junxin,Xu Wenbin,Wang Weikai,Yang Hanshen,Song Chenxin,Qiu Pengcheng,Zhao Chenchen,Fang Weifeng,Zhu Jinjin,Fan Shunwu,Liu Zhaoming,Tang Ruikang,Zhao Yueqi,Fang Xiangqian
In the elderly population, dysregulated cellular behaviour during the healing process impacts tissue regeneration after injury. Early in the regeneration process, pro-inflammatory macrophages contribute to immune imbalance, while in later stages, senescent stem cells reduce regenerative capacity. Here we demonstrate that nicotinamide adenine dinucleotide (NAD + ) can reprogramme both types of dysfunctional cell. We developed a spatiotemporal-adaptive nanotherapeutic system for the delivery of NAD + into selected cells during different phases of tissue repair. By replenishing intracellular NAD + pools, this system reshapes the multicellular regeneration niche, by metabolically rewiring pro-inflammatory macrophages towards a pro-repair phenotype during the early phase, and enhancing the differentiation capacity of senescent stem cells at later stages. This strategy effectively restored impaired bone regeneration in osteoporotic mice and accelerated skin wound healing. Our work presents a spatiotemporal-adaptive nanomedicine platform that bridges cell metabolism, nanomedicine and regeneration therapy.
研究领域:
再生医学
·
纳米医学
·
老年医学
·
代谢生物学
·
免疫调节
·
干细胞生物学
·
骨科学
·
创伤修复
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Wei Jia,Zibian Fan,An Du,Lin Shi
Ultrahigh-pressure homogenization is a powerful technique for accelerating Baijiu ageing. In this study, the molecular mechanism of artificial ageing was thoroughly investigated utilizing foodomics combined with a mass spectrum information optimization program. Various characterizations of compounds in the ageing process were performed through ultra high performance liquid chromatography quadrupole-orbitrap high resolution mass spectrometry (UHPLC-Q-Orbitrap) based colloid theoretics. High-pressure shear grinding provides a high-pressure and high-temperature environment for Baijiu, accelerating the formation of flavour compounds and the decomposition of odour and harmful substances, thus improving the sensory characteristics of Feng-flavour Baijiu and shortening the ageing time. A total of 2069 substances were identified according to fragment spectra and Bayesian network model-based peak distribution. Molecules with VIP > 1, including 14 esters, 10 acids, 4 ketones, 3 aldehydes, 2 alcohols and 2 phenols, were quantified as potential markers of chemometric analysis. A novel prediction model for Baijiu ageing degree was furnished, and ageing years of Feng-flavour Chinese Baijiu were accurately predicted by factor analysis with KMO 0.784 and a polynomial regression model with r 0.94. The results demonstrated that in 400 bar, fresh distilled Baijiu could reach a natural ageing effect of 6.43 years, which was consistent with the sensory evaluation.
研究领域:
食品科学
·
分子生物学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）