WATER RESEARCH
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2025
·
IF 12.4
Ji Qi,Shanshan Zhao,Jian Chen,Qiwei Guo,Yirong Hong,Fangang Meng
Currently, chemicals or nanoparticles are widely used for modifying membranes to improve their antifouling properties. However, the chemicals released, particularly during long-term water or wastewater filtration, are highly toxic to the environment and humans. Herein, an herb-inspired, green antibacterial membrane with exceptional sustainable antifouling properties was developed using aloin. The resultant membranes exhibited excellent bacterial inactivating efficiency because of the electrostatic interactions between the amine groups on the membrane and the bacterial cells, which contributed to cell deformation. The aloin molecules also significantly increased reactive oxygen species levels, causing oxidative damage to bacterial cells. Moreover, the functional decorative layer, which exhibited remarkable resistance to bacterial adhesion because of the abundant hydroxyl, carbonyl, and amino groups in aloin, endowed the as-prepared membranes with strong polarity, reducing bacterial adhesion and biofilm formation. When applied in a membrane bioreactor, the aloin-modified membranes demonstrated a >27.0% lower fouling rate than commercial microfiltration membranes. Overall, the successful fabrication strategy and material features described offer a green alternative for membrane development and provide new avenues for the design of healthcare materials such as wound dressings.
研究领域:
环境科学
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生物医学工程
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材料科学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2021
·
IF 4.539
Zhi-feng Cai,Chen-hua Deng,Jiao Wang,Yao Zuo,Jing-long Wu,Xiao-peng Wang,Tian-zhi Lv,Yuan-yuan Wang,Deng-yun Feng,Jie Zhao,Cai-feng Zhang,Ji-ming Zhang
Aloin is a natural medicine, which possesses the functions of enhancing immune function, anti-tumour, detoxification, anti-bacterial, anti-stomach damage, liver protection and skin protection. Excessive use will cause side effects. Thus, sensitive aloin determination is very necessary in daily life. As far as we know, there are rare reports about aloin sensing through fluorescent methods. In this work, a simple method for the synthesis of fluorescence silver nanoclusters (Ag NCs) was demonstrated by using histidine (His) as the templating agent and ascorbic acid as the reducer. The as-prepared silver nanoclusters (Ag NCs@His) possessed excellent water solubility and dispersion. Detailed research illustrated that the FL intensities of Ag NCs@His were linearly quenched in the presence of aloin (varying from 0.5 to 200 µM). The corresponding detection limit reached 0.052 µM. The static quenching and inner filter effect were the main fluorescence quenching mechanisms. This fluorescent sensor has been successfully employed for the detection of aloin in natural samples.
研究领域:
分析化学
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药物分析
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生物医学
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产品关联
DOI
高影响因子
MOLECULES
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2018
·Vol. 23
·期 3
·517
·
IF 3.098
Xuan Luo,Haowei Zhang,Xiduan Wei,Mengjuan Shi,Ping Fan,Weidong Xie,Yaou Zhang,Naihan Xu
Numerous herbal-derived natural products are excellent anti-inflammatory agents. Several studies have reported that aloin, the major anthraquinone glycoside obtained from theAloespecies, exhibits anti-inflammatory activity. However, the molecular mechanism of this activity is not well understood. In this report, we found that aloin suppresses lipopolysaccharide-induced pro-inflammatory cytokine secretion and nitric oxide production, and downregulates the expression of tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). Aloin inhibits the phosphorylation and acetylation of the NF-κB p65 subunit by suppressing the upstream kinases p38 and Msk1, preventing LPS-induced p65 translocation to the nucleus. We have also shown that aloin inhibits LPS-induced caspase-3 activation and apoptotic cell death. Collectively, these findings suggest that aloin effectively suppresses the inflammatory response, primarily through the inhibition of NF-κB signaling.Keywords:inflammation;aloin;NF-κB;macrophages;apoptosis
研究领域:
药理学
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免疫学
·
细胞生物学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
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2018
·Vol. 42
·期 4
·1925-1934
·
IF 2.784
Yunfei Ma,Tuo Tang,Lili Sheng,Ziqian Wang,Hong Tao,Qing Zhang,Yao Zhang,Zhilin Qi
The anti‑inflammatory effects of aloin, a bioactive ingredient extracted from Aloe vera, have been described previously. The present study aimed to assess these effects and explore the underlying molecular mechanisms. RAW264.7 cells were incubated with different doses of aloin (100, 150 and 200 µg/ml) and lipopolysaccharide (LPS; 100 ng/ml) for the indicated times. Then, inducible nitric oxide synthase (iNOS) and cyclooxygenase‑2 expression levels were detected by western blot analysis and reverse transcription polymerase chain reaction (RT‑PCR).The concentrations of inflammatory cytokines in the cell culture supernatant were determined by ELISA. Total nitric oxide (NO) assay and reactive oxygen species (ROS) kits were used to detect NO and ROS levels, respectively. Mitogen‑activated protein kinase, nuclear factor κB and Janus kinase‑signal transducer and activator of transcription (JAK‑STAT) pathway activation were verified by western blot analysis. Confocal and nucleocytoplasmic separation experiments were used to detect STAT nuclear translocation. It was identified that aloin decreased the level of LPS‑induced iNOS expression, inhibiting the release of interleukin (IL)‑1β, IL‑6, tumour necrosis factor‑α and NO dose‑dependently. Mechanistically, aloin suppressed LPS‑induced JAK1‑STAT1/3 activation and STAT1/3 nuclear translocation. Additionally, LPS‑induced ROS production was inhibited by aloin. Collectively, these data suggest that aloin attenuated LPS‑induced inflammation by inhibiting ROS‑mediated activation of the JAK1‑STAT1/3 signalling pathway, thereby inhibiting the nuclear translocation of STAT1/3 in RAW264.7 cells. The present study provides an experimental basis for the clinical application of aloin in inflammatory‑associated diseases.
研究领域:
免疫学
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药理学
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细胞生物学
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分子生物学
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DOI
PubMed
高影响因子
Biomolecules
·
2025
·Vol. 15
·期 7
·1052
·
IF 4.8
Guo Wei,Chuanliang Wang,Rui Wang,Peng Zhang,Xuhui Geng,Jinhua Li,Abbas Ostovan,Lingxin Chen,Zhihua Song
Aloin, a kind of active phenolic component, is sourced fromAloe vera. Recently, the determination of aloin has received enormous attention, owing to its positive performance (including anti-tumor, antibacterial, detoxification, liver protection, anti-stomach damage, and skin protection activities) and painful side effects (increased carcinogenicity caused by excessive use of aloin) impacting human health. This investigation was inspired by the good fluorescence properties of carbon dots (CDs); CD-based sensors have aroused a great deal of interest due to their excellent sensitivity and selectivity. Thus, it is of great significance to develop novel CD-based sensors for aloin determination. Herein, N,F-CDs were designed and synthesized through a convenient hydrothermal strategy; the synthesized N,F-CDs possessed good fluorescence performance and a small particle size (near 4.3 nm), which demonstrated the successful preparation of N,F-CDs. The resulting N,F-CDs possessed a large Stokes shift and could emit a highly stable green fluorescence. The fluorescence of the N,F-CDs could be effectively quenched by aloin through the inner filter effect. Furthermore, the synthesis procedure was easy to operate. Finally, the N,F-CD-coated test strips were fabricated and combined with a miniaturized fluorimeter for the fluorescence detection of aloin via the inner filter effect for the first time. The N,F-CD-coated test strips were fabricated and used for the fluorescence sensing of aloin, and the results were compared with a typical ultraviolet (UV) method. The N,F-CD-coated test strips exhibited high recovery (96.9~106.1%) and sensitivity (31.8 nM, n = 3), good selectivity, low sample consumption (1 μL), high speed (5 min), good stability, and anti-interference properties. The results indicate that N,F-CD-coated test strips are applicable for the quantitative determination of aloin in bovine serum, orange juice, and urine samples.
研究领域:
分析化学
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生物传感
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纳米材料
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药物分析
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临床诊断
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Mohan Li,Na Gan,Dian Zhou,Yi He,Fang Geng,Wei Wang,Di Wu
Aloe dairy products have occupied a certain market share, and the interaction between the small bioactive molecules contained in aloe and milk proteins during product processing profoundly affects the performance of dairy products. In this study, two typical and potentially dangerous bioactive small molecules of aloe, aloin (AA) and aloe-emodin (AE), were selected to investigate their interaction mechanism with β -lactoglobulin ( β -La). Computer simulations showed that AA/AE had the potential to form complexes with β -La, and the β -La-AA system exhibited a stronger binding affinity than the β -La-AE system. Fluorescence analysis showed that the binding affinity values of AA to β -La at 298 K were deduced to be (4.184 ± 1.06) × 10 4 M −1 , meaning medium-strength interactions, comparatively, AE to β -La was weaker, were deduced to be (3.15 ± 1.25) × 10 3 M −1 . In addition, the thermodynamic analysis found that ΔH 0 and ΔS 0 were positive in both the β -La-AA system and the β -La-AE system, which implies that the main driving force for the combination of AA/AE and β -La to form the ground state complex is the hydrophobic force. The conformation changes in β -La brought about by binding to AA/AE may alter the properties and functions of the protein, such as surface hydrophobicity and oxidation resistance. Analysis shows that β -La is potentially present in whey as an AA/AE carrier during the production of aloe milk.
研究领域:
食品科学
·
生物化学
·
分子生物学
·
营养学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）