RNA plays a crucial role in biochemical processes. RNAs with abasic sites (AP-RNA), as one of RNA lesions, will cause serious negative consequences. Efficient and sensitive detection of AP-RNA deserves but does not receive enough attention. Water-soluble uridine triphosphate (UTP)-capped Mn-doped ZnS quantum dots (QDs), which have excellent room-temperature phosphorescence (RTP) properties, were prepared by wet-chemical method. The UTP on the surface of QDs could form a precipitation complex with amiloride (AMI) to quench the RTP. Meanwhile, abasic double-stranded RNA (AP-dsRNA) with a high affinity to AMI could dissociate AMI from the surface of UTP-QDs, resulting in RTP recovery. In this process, a highly sensitive and selective RTP-detection system for AP-dsRNA was constructed with AMI as the modulation factor and UTP-QDs as the luminescence matrix. RTP was turned off and on for the determination of AP-dsRNA, and the interference of background fluorescence and scattered light was effectively avoided during complex formation and aggregation. Steric hindrance of the conformation of AP-dsRNA provided efficient selectivity. The detection limit for AP-dsRNA was 0.86 nM, the relative standard deviation was 2.1%, and the recovery of biological samples with AP-dsRNA addition ranged from 95% to 103% at optimal conditions. The system is expected to provide new methods for environmental monitoring, early disease screening, and evaluation of gene editing efficiency.
Construction of Bi 2 FeNbO 7 /bisulfite system for abatement of pharmaceutical residue was achieved. An attempt to synthesize Bi 2 FeNbO 7 through hydrothermal technique was confirmed by X-ray diffraction. The magnetic field experiment revealed that Bi 2 FeNbO 7 possessed a saturation magnetization of 6.99 emu/g, indicating magnetic attributes of Bi 2 FeNbO 7 . Scanning electron microscopy images showed that Bi 2 FeNbO 7 exhibited regular octahedra in the size of 200–300 nm. In a self-made device, the activation of sodium bisulfite using Bi 2 FeNbO 7 for the disposal of amiloride has been carefully explored. The effects of solution pH, sodium bisulfite concentration, Bi 2 FeNbO 7 dosage, amiloride concentration, coexisting ions, and water matrix on the performance of Bi 2 FeNbO 7 /bisulfite system was investigated. The catalytic performance of Bi 2 FeNbO 7 /bisulfite to degrade amiloride was considerably higher than that of traditional iron oxides . The maximum removal efficiency of amiloride was 97.9% in Bi 2 FeNbO 7 /bisulfite process. The involvement of Fe might be crucial for activating bisulfite to create active species. The dominating radical in Bi 2 FeNbO 7 /bisulfite process was identified as SO 3 •‒ . With the help of UHPLC/MS/MS, three new degradation products of amiloride were found. Dehalogenation and deamination of amiloride might account for the formation of these transformation products . This work provides a highly efficient Bi 2 FeNbO 7 /bisulfite process for the disposal of pharmaceutical pollutants in water treatment .
Wanchao Chen,Yinran Xiong,Wenming Wang,Ting Wu,Long Li,Qidi Kang,Yiping Du
A simple and sensitive fluorescence method has been developed for the determination of amiloride (AMI) in pharmaceutical tablet and human serum with a portable, cost-effective, and easy-to-operate fluorescence system. The fluorescence system was assembled with some optical and electronic devices mainly including a 370 nm light-emitting-diode (LED) as light source, a fiber spectrometer and a Raspberry Pi computer. With the system, AMI produced a strong fluorescence emission at 413 nm, and the emitted intensity can maintain good stability with in a wide pH range from 2 to 8. The proposed method was successfully applied to the determinations of AMI in pharmaceutical tablet and human serum. Their detection limits were 1.67 ng mL −1 and 1.43 ng mL −1 respectively, and the recovery was in the range of 94.06–114.0%. Those obtained results proved that the proposed methods combined with the developed fluorescence system could be employed for the routine analysis of amiloride in pharmaceutical tablet and human serum, especially for the fast analysis of them under field conditions.
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