CARBOHYDRATE POLYMERS
·
2015
·
IF 4.074
Xianglong Li,Huanhuan Chen,Bin Yang
Centrifugal spinning and electrospinning have proved to be effective techniques for fabricating micro-to-nanofibers. However, starches of amylopectin content above 65% cannot be fabricated to fiber by electrospinning. This paper is focus on the centrifugal spinnability of amylopectin rich starches. We investigated the amylopectin content of starches by Dual-wavelength colorimetry, studied the rheological properties of starch dopes to determine entanglement concentration ( c e ) by rotary rheometer. Results indicated that amylopectin rich native corn and potato starches, which with amylopectin content higher than 65%, were suitable for centrifugal spinning to micro-to-nanofibers. Additionally, starch-based fibers were successfully fabricated from the amylose rich corn starch as well. Rheological studies showed that the entanglement concentration ( c e ) of starch solution was crucial for successful centrifugal spinning.
研究领域:
材料科学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2017
·Vol. 135
·期 6
·45810
·
IF 1.86
Xianglong Li,Teng Hou,Yishen Lu,Bin Yang
In the present study, we provide a method for controlling the surface morphology of centrifugally spun starch-based fibers by adjusting the ratio of amylopectin/amylose in starches and combining with a hot blast temperature. The effects of hot blast temperature, amylopectin, and amylose on fiber surface morphologies are investigated. Scanning electron microscopy is used to characterize the morphology of the prepared fibers. The results show that fibers with burr-shaped nanostructures on the surface can be fabricated by adding amylopectin to starches and are promoted by increasing the hot blast temperature. However, amylose in starches plays the role of smoothing the fiber surfaces. X-ray diffraction reveals that the fibers are amorphous. Through Fourier transform infrared spectroscopy analysis, it was found that some physicochemical changes occur during centrifugal spinning. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018 , 135 , 45810.
研究领域:
材料科学
·
高分子化学
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产品关联
DOI
高影响因子
BIOMATERIALS
·
2012
·
IF 7.404
Yanfang Zhou,Bin Yang,Xianyue Ren,Zhenzhen Liu,Zheng Deng,Luming Chen,Yubin Deng,Li-Ming Zhang,Liqun Yang
A series of hyperbranched cationic amylopectin derivatives conjugated with 1,2-ethylenediamine, diethylenetriamine and 3-(dimethylamino)-1-propylamine residues, named as EDA-Amp, DETA-Amp and DMAPA-Amp, were synthesized by the N,N’- carbonyldiimidazole activation method at room temperature. Their structures were characterized by FTIR and 1 H NMR analyses, and their buffering capability was assessed by acid-base titration. The amylopectin derivatives exhibited better blood compatibility and lower cytotoxicity when compared to branched polyethyleneimine (bPEI) in the hemolysis and MTT assays . Atomic force microscopy and optical microscopy confirmed that the amylopectin derivatives exhibited lower damage for erythrocytes than bPEI. The amylopectin derivatives could bind and condense plasmid DNA (pDNA) to form the complexes with the size ranging from 100 to 300 nm. The resultant complexes showed higher transfection efficiency in 293T cells than in A549 cells. The DMAPA-Amp derivative-mediated gene transfection for Forkhead box O1 exhibited higher protein expression than that of the EDA-Amp and DETA-Amp derivatives in 293T cells, which was analyzed by western blot , flow cytometry and Hoechst staining assay. On the basis of these data, amylopectin derivatives exhibit potential as nonviral gene vectors .
研究领域:
药学
·
分子生物学
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生物技术
·
基因治疗
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 9
·期 4
·1082-1088
·
IF 5.344
Lingshu Kong,Zijian Gao,Xinyao Li,Guanghui Gao
Self-adhesiveness is highly desirable for conformal and seamless wearable electronics. Here, a starch-tackifying method is proposed to obtain adhesive and robust hydrogel conductors with the assistance of amylopectin (Amy). The conductive hydrogels are composed of Amy/poly(acrylamide–acrylic acid) polymer networks, which can be assembled into wearable sensors. The hydrogels rely on physical interactions such as hydrogen bonding that can be generated on the surface of the material, including skin, to exhibit robust and repeatable self-adhesive behaviors. Besides, the construction of a covalent and dynamic dual cross-linking network endows the hydrogel with good mechanical properties to bear repeated stretching and flexible deformation. In particular, the hydrogel is assembled into a wearable stretchable and compressible sensor and exhibits a repeatable and stable resistance signal variation for detecting both large and tiny scale human activities and physiological signals, such as bending of joints, speaking, walking, and jumping. Accordingly, the amylopectin-enabled skin-mounted hydrogel sensor can be considered as an ideal choice for human movement monitoring and personal health diagnosis.
研究领域:
材料科学
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生物医学工程
·
传感器技术
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产品关联
DOI
PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
·
2023
·
IF 6
Rujun Dai,Yiyan Gao,Yibo Sun,Kai Shi,Guanghui Gao,Huixuan Zhang
Conductive hydrogels with excellent flexibility, durability and functional diversity have great development prospects in the field of flexible strain sensors. However, most hydrogels inevitably lose their properties (such as elasticity, electrical conductivity, fatigue resistance, etc.) when frozen and crystallized in low temperature environments, severely hindering their application in extreme environments. Hence, the hydrogel was successfully prepared by introducing amylopectin, polyvinyl alcohol, lithium chloride and glycerol, which possessed the ability of freezing resistance and water retention. The interaction between amylopectin and polyvinyl alcohol formed hydrogen bonds, which endowed the hydrogel excellent tensile strain (608%) and stress (581 kPa). In addition, the hydrogel possessed significant water retention and excellent freeze resistance due to the introduction of glycerol and lithium chloride. Simultaneously, the PGLA hydrogel was placed into mice for 14 days without redness, swelling, allergy and other reactions, indicating the hydrogel with biocompatibility. More importantly, the PGLA hydrogel could detect not only the large strain caused via joint campaign but also the small strain exhibited by weak mastication. This research provides a new way to the development of wearable sensors with the ability to operate in low-temperature environments and biocompatibility.
研究领域:
材料科学
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生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-14
·
IF 15.7
Fan Rong,Guan Zeyuan,Zhou Guanghong,Yang Xi,Zhang Fei,Wu Menglong,Wang Xuecui,Liu Jian,Chen Pei,Liu Yanjun,Zhang Delin,Yin Ping,Yan Junjie
Amylopectin, the primary form of starch in plant leaves, seeds and tubers, features a tree-like architecture with branched glucose chains. Excess branches result in the formation of soluble phytoglycogen instead of starch granules. In higher plants and green algae, the debranching enzyme isoamylase ISA1 forms either homomultimer or hetero-multimer with ISA2 to facilitate branch trimming and starch granule formation, but the molecular basis remains largely unknown. In this study, we reconstitute the rice OsISA1-ISA2 complex in vitro and determine the cryo-EM structures of the OsISA1 homodimer, as well as the malto-oligosaccharide (MOS)-free and MOS-bound OsISA1-ISA2 heterocomplex. The OsISA1 dimer shows a tail-to-tail rod-like architecture, whereas the OsISA1-ISA2 complex mainly exhibits as a trimer, with OsISA2 flanking on the N-terminal segments of the dimeric OsISA1. Combined with comprehensive biochemical analyses, these structural data elucidate the organization of the ISA1-ISA2 heterocomplex in higher plants and demonstrate how ISA1 and ISA2 cooperate during amylopectin biosynthesis.
研究领域:
植物生物学
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结构生物学
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生物化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2026
·
IF 5.6
Yaxin He,Xin Xie,Yanghui Chen,Wenchen Tao,Junjie Yu,Meng Huang,Yan Lin,Juan Chen,Jian Liu,Xian Zhang
Adipose tissue metabolic dysfunction plays a central role in the development of obesity and related metabolic disease. Luteolin is a natural flavonoid with antioxidant, anti-inflammatory, antidiabetic, and antiobesity activities, while it is limited by poor solubility, bioavailability, and stability. This study developed a whey protein isolate (WPI)–Pueraria lobata amylopectin (PLA) composite gel, characterized by a dense 3D network and enhanced mechanical strength. The WPI-PLA gel efficiently encapsulated luteolin in a stable amorphous form and exhibited uniform particle size and excellent colloidal stability. This encapsulation improved the light, storage, and thermal stabilities of luteolin; enhanced its antioxidant activity due to improved dispersion; and enabled its preferential intestinal release. Encapsulated luteolin promoted the expression of thermogenic genes UCP1, Pgc-1α, Prdm16, and Cidea and protein levels of UCP1 and catalase in brown-differentiated C3H10T1/2 cells and inhibited the expression of lipopolysaccharide-induced inflammatory cytokines Il-6, Tnf-α, and Il-1β in RAW264.7 macrophages. In differentiated white 3T3-L1 adipocytes, WPI-LU-PLA restored insulin sensitivity impaired by macrophage-conditioned medium. This reversal was indicated by the enhanced expression of the insulin sensitivity-related genes GLUT4 and Irs1, along with increases in GLUT4 and p-Akt protein levels. In conclusion, the WPI-PLA composite gel enhances the stability, bioaccessibility, and bioactivity of luteolin, suggesting potential for functional foods targeting metabolic dysfunction in adipose tissues.
✓ 已验证
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2026
·
IF 13.2
Xinyi Chen,Liping Fu,Ye Xu,Lujie Cheng,Zijian Huang,Wenhui Zhang,Qisen Xiang,Yang Liu,Yuting Tian,Yingting Zhao
This study investigated the synergistic mechanism of partial debranching and microwave treatment on the complexation between highland barley amylopectin and 2-ethyl-6-methyl pyrazine (EMP) in an aqueous system, a key roasted aroma compound in tsampa. Pullulanase-mediated partial debranching optimized the chain length distribution by reducing steric hindrance, which alone enhanced EMP binding. Subsequent microwave treatment (300, 500, 700 W) further promoted complexation through distinct, power-dependent mechanisms that fundamentally differ from conventional water bath heating. Unlike the random thermal motion of water bath heating, microwave irradiation induced high-frequency dipole rotation and orientational polarization, driving starch chain rearrangement and conformational tightening. Especially, at 700 W, partial chain cleavage generated linear fragments that readily formed V -type inclusion complexes, doubling EMP encapsulation compared to native starch. Molecular dynamics simulations confirmed that microwave treatment induced tighter chain folding and stronger intermolecular interactions than water bath heating. This work demonstrates that microwave irradiation actively reorganizes starch architecture rather than merely providing thermal energy, offering a novel strategy for starch-based flavor encapsulation.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF CEREAL SCIENCE
·
2025
·
IF 3.7
Yongliang Bai,Junwei Yin,Zuanhao Liang,Yu Xia,Shuyan He,Ming Yu,Haiqiang Chen
To investigate the impact of germination on the development of deep-processed products from highland barley starch, this study systematically examined changes in the structure, composition, physicochemical properties, and related enzyme activities of starch during germination. After 72 h of germination, the contents of total starch and amylopectin in highland barley starch decreased significantly ( p < 0.05). The activities of α-amylase and β-amylase increased dramatically by 19.6-fold and 6.3-fold, respectively. Concurrently, the gelatinization enthalpy ( ΔH ) of the starch decreased significantly, whereas the syneresis rate showed a significant increase ( p < 0.05). Correlation analysis revealed that the germination treatment enhanced the activities of α-amylase and β-amylase in highland barley, which promoted the enzymatic degradation of amylopectin. This led to a significantly increased starch solubility, syneresis rate, and initial gelatinization temperature( T 0 ), while decreasing starch transparency, termination gelatinization temperature ( T c ), peak temperature ( T p ), and Δ H . The results demonstrate that germinated highland barley starch is suitable for noodle products.
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Wentao Wang,Yutong Xia,Mang Wu,Min Wu,Jianbing Chen
Nonconjugated fluorescent polymers devoid of large π–π conjugated structures have received considerable attention due to their significant academic importance and broad application potentials in various fields. Herein, we report an effective strategy to fabricate multifunctional fluorescent amylopectin derivatives and reveal their unique aspects of aggregation-induced emission (AIE), cryogenic long-persistent phosphorescence (~6 s) and excellent processabilities characteristics, which are extremely different from traditional luminogens. These amylopectin- graft -poly( n -butyl acrylate- co -1-vinylimidazole) copolymers (Amylopectin-BVs) prepared by the grafting-from method employing RAFT and experienced subsequently with metal-ligand cross-linking. Specifically, clustering-triggered fluorescent emission or cryogenic long-persistent phosphorescence of amylopectin could be achieved by the aggregation of oxygen and nitrogen atoms along with conformation rigidification, which shows great promise in optoelectronic and biological applications.
研究领域:
材料科学
·
光学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Polymers
·
2019
·Vol. 11
·期 2
·342
·
IF 3.164
Shuyan Zhang,Jie Zhu,Yujia Liu,Shui-Yang Zou,Lin Li
Starch-based materials with reinforced properties were considered as one of the most promising materials to replace the petro-based packaging products, and actually, the molecular structures of starch usually determined the structures and properties of end-used starchy products. Here, starch-based nanocomposites were fabricated by starch esters derived from native starches with different amylose contents and organically modified montmorillonite (OMMT). The fractured surface under scanning electron microscopy (SEM) exhibited wrinkles formed by macromolecular aggregation owing to the interaction competition between the plasticizer and nanofiller with the starch ester. The more intense interaction within amylopectin-rich films promoted the formation of much randomly exfoliation of OMMT observed by Transmission electron microscopy (TEM). As the amylose content increased, the interaction between the starch ester and the nanofiller was weakened, leading to the dispersion morphology of an ordered arrangement and partly intercalated structures in the dimension of 12.92 to 19.77 nm. Meanwhile, such interaction also affected both the inner ordered structure integrity of starch ester and the layer structure consistency of nanofiller according to X-ray diffraction results. Further, the stronger interaction between amylopectin and the nanofiller endowed higher thermal stability to the amylopectin-rich starch-based nanocomposites. In short, these results are beneficial for the application of starch-based nanocomposites in the food packaging industry by regulating the interaction between starch and nanofillers.Keywords:starch-based nanocomposites;amylose/amylopectin ratio;hierarchical structure;thermal property
研究领域:
材料科学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CRYSTAL RESEARCH AND TECHNOLOGY
·
2021
·Vol. 56
·期 8
·2100012
·
IF 1.639
Qinjiang Zhao,Lihong Zhou,Jie Du,Guanghui Wang,Xiangjun Pei
As an ecological and environment-friendly building material, sticky rice mortar has attracted the attention of many researchers. Amylopectin is the most important organic matter of it, which can improve the mechanical properties and durability of sticky rice mortar by inducing mineralization of calcium carbonate (CaCO 3 ). However, amylopectin induced formation of CaCO 3 has not been reported. In this paper, mineralization of CaCO 3 induced by gelatinized amylopectin is investigated. For comparison, the different morphologies of CaCO 3 are studied in the presence of Mg 2+ , Fe 3+ . Moreover, these CaCO 3 products with different morphologies are used to clean the waste water. The results indicate that gelatinized amylopectin is conducive to formation of pumpkin-like vaterite. It forms uniform rod and dumbbell-shaped calcite in the presence of Mg 2+ , form calcite with stepped depressions in the presence of Fe 3+ . The adsorption of Pb(II) can reach extremely high level (1445.86 mg g −1 ) with the rod-like calcite.
研究领域:
材料科学
·
环境科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）