CHROMATOGRAPHIA
·
2012
·Vol. 75
·期 23
·1347-1354
·
IF 1.195
Sun Baishen,Li Xiaofang,Jin Zhaolei,Tian Lili,Wang Fang,Liu Guihua,Tang Shouwan,Pan Fuyou
Four regioselectively modified amylose derivatives with three different substituents at the 2-, 3-, and 6-positions were prepared and their enantioseparations in HPLC were examined. Investigations indicated that the nature as well as the arrangement of the substituents significantly influenced their enantioseparations and each derivative exhibited characteristic chiral recognition. Amylose 2-benzoyl-3-(3,5-dimethylphenylcarbamate or 3,5-dichlorophenylcarbamate)-6-(( S )-1-phenylethylcarbamate) exhibited chiral resolving abilities comparable to the commercial available amylose tris(3,5-dimethylphenylcarbamate)-based column, Chiralpak AD and the racemic compounds shown in this study were most effectively resolved on these two derivatives. The influence of mobile phase on chiral resolution was also examined.
研究领域:
化学
·
分析化学
·
色谱技术
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产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2016
·
IF 4.219
Ying Zhou,Youxin Ye,Wenwen Zhang,Songling Li,Jing Chen,Shiting Wang,Defu Li,Changdao Mu
The oxidized amyloses with different carboxyl content were prepared to include linalool for antimicrobial activity in aqueous environment. The results show that linalool can be effectively reserved from volatilization through encapsulation into amylose and oxidized amyloses. The inclusion ability of oxidized amyloses towards linalool is decreasing with the increase of oxidation level due to the depolymerization of amylose. However, the solubilization effect of oxidized amyloses to linalool is enhanced efficiently owning to the high water solubility of oxidized amyloses. It is interesting that the inclusion complexes have good antimicrobial activity in aqueous environment. Linalool solubilized by oxidized amyloses presents better antimicrobial performance than that solubilized by amylose, mainly resulting from that amylose-linalool inclusion complex would aggregate and retrograde fast in aqueous solution, which is disadvantageous for the release of linalool. The study suggests that oxidized amylose is a promising solubilizer and carrier of linalool for antimicrobial activity in aqueous environment.
研究领域:
食品科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 18
·3366
·
IF 5.2
Xinyue Liu,Zhuangzhuang Sun,Wenqing Zhao,Jiayu Zheng,Wei Liang,Wenhao Li
To explore the effect of amylose within starch granules on the efficiency of starch hydrolysis by acid, we chose the warm water extraction method to treat red adzuki bean starch to obtain different degrees of amylose removal granule models and to prepare samples in combination with acid hydrolysis. The amylose content was reduced after acid hydrolysis, reducing the peak viscosity (2599–1049 cP), while the solubility was significantly increased. In contrast, the short-chain content of the deamylose–acid hydrolysis samples was reduced considerably, exacerbating the trend towards reduced starch orderliness and increased solubility. This work reveals the granular structure of starch from the point of view of deamylose and contributes to a thorough understanding of the mechanisms of acid hydrolysis. It might add to knowledge in starch science research and industrial applications for the acid processing of starch-based foods, particularly with regard to the most important factors controlling the structure and function of starch.Keywords:deamylose;dual modification;red adzuki bean starch;multiscale structure
研究领域:
食品科学
·
淀粉化学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 8
·期 15
·3010-3015
·
IF 5.344
Yanbin Sun,Xianwen Wang,Linxin Fan,Xianli Xie,Zhaohua Miao,Yan Ma,Tao He,Zhengbao Zha
The famous chromogenic reaction between starch and iodine has been widely used for chemical analysis since its first discovery in the year of 1814 while it is seldom utilized in biomedical applications. Inspired by their high iodine content and strong optical absorbance in the near infrared (NIR) region, monodisperse amylose–iodine nanoparticles (AM–I NPs), synthesized by simple mixing of amylose NPs and KI–I2 solutions, were explored as a new class of high-performance antibacterial agent. Benefiting from the broad-spectrum antibacterial property of iodine and photothermal effect of the amylose–iodine complex, the obtained AM–I NPs exhibited an excellent photothermal-enhanced sterilization effect for both Gram-negative Escherichia coli (E. coli) and Gram-positive methicillin-resistant Staphylococcus aureus (MRSA). For instance, upon incubation with AM–I NP suspensions (30 μg mL−1) plus NIR laser irradiation (808 nm, 1.33 W cm−2, 5 min), the relative survival rates of E. coli and MRSA were only 1.2% and 1.7%, respectively. In addition, the AM–I NPs depicted better biocompatibility in vitro than that of KI–I2 solution, indicating their safety for potential biomedical applications in vivo. This proof-of-concept study revealed the antibacterial applications of a traditional starch-iodine complex and is expected to provide insights into the design and development of efficient broad-spectrum antibacterial agents.
研究领域:
生物医学工程
·
材料科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanomaterials
·
2017
·Vol. 7
·期 5
·110
·
IF 3.553
Zhuo Wu,Xiao-Lin Xu,Jun-Zhao Zhang,Xu-Hong Mao,Ming-Wei Xie,Zi-Liang Cheng,Lie-Jing Lu,Xiao-Hui Duan,Li-Ming Zhang,Jun Shen
Amylose is a promising nanocarrier for gene delivery in terms of its good biocompatibility and high transfection efficiency. Small interfering RNA against survivin (survivin-siRNA) can cause tumor apoptosis by silencing a hepatocellular carcinoma (HCC)-specific gene at the messenger RNA level. In this study, we developed a new class of folate-functionalized, superparamagnetic iron oxide (SPIO)-loaded cationic amylose nanoparticles to deliver survivin-siRNA to HCC cells. The cellular uptake of nanocomplexes, cytotoxicity, cell apoptosis, and gene suppression mediated by siRNA-complexed nanoparticles were tested. The results demonstrated that folate-functionalized, SPIO-loaded cationic amylose nanoparticles can mediate a specific and safe cellular uptake of survivin-siRNA with high transfection efficiency, resulting in a robust survivin gene downregulation in HCC cells. The biocompatible complex of cationic amylose could be used as an efficient, rapid, and safe gene delivery vector. Upon SPIO loading, it holds a great promise as a theranostic carrier for gene therapy of HCC.Keywords:amylose;small interfering RNA;magnetic resonance imaging;superparamagnetic iron oxide nanoparticles
研究领域:
纳米技术
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基因治疗
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癌症研究
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分子生物学
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Rui Wang,Mei Li,Jinguang Liu,Fenfen Wang,Jing Wang,Zhongkai Zhou
The effect of dual modifications of pullulanase debranch and propionylation on rice starch property was investigated in this study. The occurrence of the propionylation in starch molecules was confirmed due to the formation of a new peak at 1750 cm −1 in FTIR spectrum and the characteristic peaks around 1.0 (methyl) and 2.2 (methylene) ppm in NMR compared with the native starch. Interestingly, because of the higher tendency of molecular retrogradation following the debranching, the current results revealed that there was no positive correlation between the amylose content (AC) and the debranching time. Debranching treatment increased the degree of substitute (DS) of the propionyl starch, and the AC was positively correlated with DS (R = 0.7753*), indicating that AC is an important element affecting propionyl reaction due to the exposure of more available hydroxyl groups during debranching. Meanwhile, debranching led to an increased crystallinity level and the crystallinity pattern changed from A-type to B-type, which is associated with the changes in their corresponding hydrolysis resistance. The NMR data also proved that the propionylation involves the replacements of the glucose in the starch structure (C-2, C-3 and C-6) with acyl groups of the desired complexes. Furthermore, the sequence of the reactions that occur in the process of propionylation and their mechanisms are proposed in the current study.
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2017
·Vol. 135
·期 6
·45810
·
IF 1.86
Xianglong Li,Teng Hou,Yishen Lu,Bin Yang
In the present study, we provide a method for controlling the surface morphology of centrifugally spun starch-based fibers by adjusting the ratio of amylopectin/amylose in starches and combining with a hot blast temperature. The effects of hot blast temperature, amylopectin, and amylose on fiber surface morphologies are investigated. Scanning electron microscopy is used to characterize the morphology of the prepared fibers. The results show that fibers with burr-shaped nanostructures on the surface can be fabricated by adding amylopectin to starches and are promoted by increasing the hot blast temperature. However, amylose in starches plays the role of smoothing the fiber surfaces. X-ray diffraction reveals that the fibers are amorphous. Through Fourier transform infrared spectroscopy analysis, it was found that some physicochemical changes occur during centrifugal spinning. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018 , 135 , 45810.
研究领域:
材料科学
·
高分子化学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Jie Zhu,Shuyan Zhang,Yujia Liu,Siqian Chen,Lin Li
The structures of starch and starch-based materials determine additives migration from material matrix. Propionylated starch derived from waxy, normal, G50 and G80 starch were selected as the matrix, the amylose effect on plasticizer (triacetin) migration as well as structural changes in hydrophobic starch-based films were discussed. The constant ( k 1 ) of first-order rate and initial release rate ( V 0 ) of triacetin migration were consistent with the increment of amylose content. Meanwhile, diffusion model disclosed that Fick's second law was apposite to characterize the short-term migration of triacetin, and larger diffusion coefficient ( D ) values of short- and long-term migration were also found in films with higher amylose content, indicating that amylose-formed structures were in favor of triacetin migration. In comparison of propionylated amylopectin , Van der Waals's interactions between propionylated amylose and triacetin were easier to be weakened with the migration of triacetin, which promoted the decrease of wavenumber of C-O-C, and enlarged the inter-planner spacing of crystalline structures, promoting the formation of amorphous structures and wrinkles and embossments in films with higher amylose content. This work confirmed that regulating the structures of starch were effective to control the migration behavior of additives from starch-based films.
研究领域:
食品科学
·
材料科学
✓ 已验证
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Jin Du,Yajing Qi,Alkassoumi Hassane Hamadou,Jian-Ya Qian,Bin Xu
The objective of this research was to investigate the influence of starch granule-associated lipids (SGALs) on retrogradation properties of buckwheat and wheat starches. According to the results, the removal of SGALs led to remarkable increase in the retrogradation enthalpy change of all starches and the strength of starch gels, as well as the density and short-range ordered structure of starch aggregates. The strength of starch gel experienced a rise from 3139.39 g to 3718.18 g in Tartary buckwheat, 2924.12 g to 3551.13 g in common buckwheat, and 1887.55 g to 2555.24 g in wheat, respectively. The removal of SGALs contributed to a decrease in the thermal stability of starches and an augmentation of amylose leaching during gelatinization process, which would strengthen the hydrogen bond interaction between starch molecules during cooling process, and promoting the rearrangement of the order structure of starch molecules. In general, these results indicated that the retention of SGALs could limit amylose leaching, then inhibited rearrangement and recrystallization of dissolved starch molecules, and ultimately delayed the short-term and long-term retrogradation process. This work further supplemented theoretical knowledge about SGALs in buckwheat and wheat starches, also provided a new perspective for regulating the physicochemical properties of starches.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2015
·
IF 2.711
Pengwu Zheng,Yuanyuan Du,Peter R. Chang,Xiaofei Ma
A supramolecular structure was initially formed between amylose and halloysite (HNT) simply by using a mechanical force. Subsequently, amylose acted as a template for the growth of TiO 2 nanoparticles. The thus-prepared amylose–HNT–TiO 2 composite was characterized using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric analyses, and transmission electron microscopy (TEM). In comparison to its counterpart HNT-TiO 2 composite, the amylose–HNT–TiO 2 composite exhibited better dispersion, a larger specific surface area, and photocatalytic activity that was more effective for the photodegradation/removal of methylene blue (MB) and the persistent organic pollutant 4-nitrophenol (4-NP) under UV irradiation. After four successive UV irradiation treatments, amylose–HNT–TiO 2 removed about 90% of 4-NP or MB.
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2013
·
IF 3.479
Yinhui Li,Shudong Lin,Jiwen Hu,Guojun Liu,Gangwei Zhang,Yuanyuan Tu,Hongsheng Luo,Wei Li
Cu 2+ /amylose assemblies of various sizes were prepared through the Cu 2+ ion induced-assembly of amylose. These assembly structures were characterized via transmission electronic microscopy (TEM), scanning electronic microscopy (SEM), dynamic light scattering (DLS), 1 H NMR analysis, fluorescence spectroscopy (FL) and UV–vis absorption spectroscopy (UV–vis). The results from these characterizations revealed the existence of a complexation effect and/or a bridging effect between the hydroxyl groups of amylose and Cu 2+ ions, and that the formation of the hydrophobic domains promoted the formation of Cu 2+ /amylose assemblies. The use of other metal ions to induce the formation of spherical, flower- and wire-like amylose assemblies was investigated as well. A preliminary investigation on the ability of amylose to capture various metal ions was also performed, and the results of this work demonstrated that amylose could bind quantitatively metal ions that were at low concentrations. This work provided an alternative strategy for the recovery of precious metals from metal ion-containing aqueous solutions and the reduction of water pollution.
研究领域:
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
STARCH-STARKE
·
2018
·
IF 2.173
Xiuting Hu,Yu Wang,Chengmei Liu,Zhengyu Jin,Yaoqi Tian
The 1-butanol-hydrochloric acid (1-butanol-HCl) degradation mechanism of high-amylose maize starch, represented by amylomaize V, is investigated using different methods. More pores are formed in the amylomaize V granules during 1-butanol-HCl hydrolysis and these pores become larger and change into cracks. Correspondingly, the average granule size slightly decreases from 13.49 to 10.87 µm after 1-butanol-HCl hydrolysis for 7 d. The crystalline areas of amylomaize V are hydrolyzed by 1-butanol-HCl and the amylose-1-butanol complex might form. In addition, the weight-average molecular weight of amylomaize V dramatically decreases from 1.25 × 10 7 to 3.57 × 10 5 Da after hydrolysis for 4 h, then slowly decreases, and stabilizes at about 2.00 × 10 4 Da after hydrolysis for 4 d, which is in accordance with the result of iodine staining analysis. High-performance anion-exchange chromatography analysis indicates that 1-butanol-HCl can hydrolyze the starch like hydrolysis patterns of both the endo-enzyme and the exo-enzyme. These results suggests that HCl in 1-butanol quickly diffuses into the whole amylomazie V granule and simultaneously attacks the crystalline and disorder areas, while the compact periphery of amylomaize V keeps the granules from disintegrating.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）