JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2023
·
IF 8.7
Junlang Li,Jilan Dong,Zhenguo Chen,Xiaoyong Li,Xiaohui Yi,Guoqiang Niu,Jiaan He,Simin Lu,Yuxiang Ke,Mingzhi Huang
Free nitrous acid (FNA) is a critical metric for stabilization of ANAMMOX but can not be directly and immediately measured by sensors or chemical measurement method, which hinders the effective management and operation for ANAMMOX. This study focuses on FNA prediction using hybrid model based on temporal convolutional network (TCN) combined with attention mechanism (AM) optimized by multiobjective tree-structured parzen estimator (MOTPE), called MOTPE-TCNA. A case study in an ANAMMOX reactor is carried out. Results show that nitrogen removal rate (NRR) is highly correlated with FNA concentration, indicating that it can forecast the operational status by predicting FNA. Then, MOTPE successfully optimizes the hyperparameters of TCN, helping TCN achieve a high prediction accuracy, and AM furtherly improves model accuracy. MOTPE-TCNA obtains the highest prediction accuracy, whose R 2 value gets 0.992, increasing 1.71–11.80% compared to other models. As a deep neural network model, MOTPE-TCNA has more advantages than traditional machine learning methods in FNA prediction, which is beneficial to maintain the stable operation and easy control for ANAMMOX process.
研究领域:
环境生物学
·
废水处理工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2019
·
IF 7.65
Yongpeng Ma,Denghui Wei,Xiaojing Zhang,Haoqiang Fu,Tao Chen,Jinping Jia
Anaerobic ammonium oxidation (Anammox) was an innovative process for nitrogen removal. In this study, CuO nanoparticles (NPs) was step-wise increasingly added to an MBR-based partial nitrification system, to investigate its feasibility for inducing Anammox and establishing autotrophic nitrogen removal system. Results showed that when CuO NPs was elevated to 5 mg L −1 , Anammox was successfully induced. The relative abundance of Nitrosomonas reached 13.73% while Candidatus Kuenenia increased to 4.79% from 0.46%, these two bacteria cooperatively contributed to the autotrophic nitrogen removal and improved the nitrogen removal rate (NRR) to 0.56 kg m -3 d −1 in 20 mg L −1 NPs. However, 50 mg L −1 NPs deeply suppressed the functional bacteria and decreased NRR to 0.14 kg m -3 d −1 . Finally, the NPs removal, transformation and adsorption in the system were evaluated. It was concluded that CuO NPs in low concentration (5 mg L −1 ) was effective for inducing Anammox and contributed to the survival of Anammox bacteria. The mechanism for inducing Anammox was attributed to the aggregation of CuO NPs which enabled the attached growth of AAOB as well as the suitable survival condition supplied by MBR.
研究领域:
环境工程
·
微生物学
·
水处理技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Lingjie Liu,Shaoqing Mo,Shaopo Wang,Jing Chang,Jianhui Zhao,Jingjie Yu
Low temperatures were a critical factor that inhibited anaerobic ammonium oxidizing bacteria (AnAOB) activity. This study demonstrated the effect of exogenous zero-valent iron (ZVI) on Anammox-hydroxyapatite (HAP) system at 17°C. The results showed that long-term addition of ZVI increased the system nitrogen removal efficiency by 8.59% and alleviated the inhibition of AnAOB by low temperature. After addition of ZVI, the activity of AnAOB and the heme c content increased by 58.20% and 4.83%, respectively. Moreover, the functional enzyme activities of NIR, HZS, and HZO increased by 94.12%, 36.36%, and 16.67%, respectively. The concentrations of quorum sensing molecules, i.e., C 6 -HSL and C 8 -HSL, increased by 191.17% and 41.54%, respectively, after ZVI addition. Metagenomic analysis revealed that the addition of 5 g/L ZVI stimulated the relative abundance relative abundance of Fe(II) metabolism-related genes in AnAOB at 17 °C, thereby contributing to the uptake, transport, utilization, and storage of Fe(II). The addition of ZVI could improve the nitrogen removal performance of the anammox-HAP system at the low temperature.
研究领域:
环境工程
·
微生物学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
npj Clean Water
·
2020
·Vol. 3
·期 1
·1-10
·
IF 4.87
Zou Chunzhen,Guo Beibei,Zhuang Xuming,Ren Liying,Ni Shou-Qing,Ahmad Shakeel,Qiao Zhuangming,Cui Zhaojie,Hong Jinglan
The effects of FeS on nitrogen removal performance and microbial community of anammox process were studied. During the start-up period, the removal efficiencies of nitrite and total nitrogen were significantly improved by FeS. The addition of FeS increased the content of iron ions in the reactor and promoted the synthesis of heme c , which was involved in the formation of various enzymes. Compared with the control, the abundance of anammox bacteria in the FeS reactor was increased by 29%, and the expression level of the nirS gene (encoding cd 1 type nitrite reductase containing heme) was nearly doubled. The content of nitrite reductase (ammonia-forming) in the community was increased by 26.4%. The difference in functional bacteria and enzyme contents in the microbial community resulted in a difference in nitrogen removal rate (NRR) between the two reactors. High-throughput results indicated that FeS increased the richness and diversity of microbial community and enhanced the metabolic function of the microbial community. The addition of FeS did not change the dominant position of Ca. Kuenenia in both reactors. But the relative abundance of heterotrophic denitrifying bacteria was reduced with FeS, which may be related to the inhibition effect of S 2− produced by FeS.
研究领域:
环境微生物学
·
污水处理
✓ 已验证
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2020
·
IF 7.632
Zhen Bi,Wenjing Zhang,Min Ni,Ge Song,Yong Huang
The anammox bacteria (AnAOB) have been suggested to perform dissimilatory Fe0/Fe2+-dependent nitrate reduction to ammonium (DNRA), with nitrite as intermediate. However, evidence at genetic and transcriptional levels for how AnAOB reduces nitrate is still lacking, making their roles in this process obscure and questionable. The short-term batch assays and long-term operation of bioreactor combined with 16S rDNA sequencing and transcription analysis were used for the first time to investigate the nitrate reduction pathway in anammox consortia. It is shown that AnAOB metabolically well grew with nitrate as the sole nitrogen source. They reduced nitrate to nitrite with highly expressed narG gene. However, the DNRA-enabling gene nrfA was not expressed by AnAOB. Abiotic reduction by Fe0/Fe2+ contributed to the nitrate and nitrite reduction to ammonium. The correlation analysis of microbial community structure and metabolic functions indicated that amino acid and carbohydrate transport and metabolism interactions in coexisting bacteria could facilitate the AnAOB growth with nitrate as the only nitrogen source. Our findings suggest that the contribution of enzymatic DNRA by AnAOB to the observed nitrate reduction has been probably overestimated in previous publications, and it should be questioned until the clear enzymatic mechanism responsible for nitrate reduction is identified.
研究领域:
微生物学
·
环境科学
·
生物技术
✓ 已验证
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Wenzhe Liu,Baiyang Chen,Yang Yang,Boqiang Li,Huimei Pan,Wang Luo
Excessive ammonia (NH 4 + ) discharge can lead to algal blooms and disrupt water sustainability, so its control is imperative. Although microbiology-triggered anammox process is promising, its application is limited due to time-consuming cultivation of specific microorganisms and need for skilled operation. To bypass these barriers, this study proposed and verified a photo-induced anammox technology that removes NH 4 + and total nitrogen (TN) from water by ultraviolet (UV)/vacuum UV (VUV)/chlorine under anoxic conditions . Under the Cl/N mass ratio of 5:1, the anoxic VUV/UV/chlorine process achieved 66.8% removal of 10 mg-N/L NH 4 + within 10 min along with 57.8% reduction in TN. Besides the evidence from TN loss, this study confirmed nitrogen gas (N 2 ) as the primary degradation product at low dissolved oxygen (DO) concentration of 2.0 mg/L. The selective conversion of NH 4 + into N 2 was mainly attributed to reactive nitrogen species (RNS, 42.5%) and reactive chlorine species (RCS, 57.5%). The TN removal efficiency was insensitive to certain variations of pH (7.0–9.0), NH 4 + concentration (1–30 mg-N/L), chloride (50–125 mg/L), and sulfate (25–100 mg/L), but sensitive to DO and bicarbonate (25–100 mg/L). Given its robustness and high efficiency, the anoxic VUV/UV/chlorine technology may serve as a potentially promising alternative for NH 4 + and TN alleviation in wastewater.
研究领域:
环境科学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
WATER RESEARCH
·
2020
·
IF 9.13
Da Kang,Yiyu Li,Dongdong Xu,Wenji Li,Wei Li,Aqiang Ding,Ru Wang,Ping Zheng
The red color is the most striking character of anaerobic ammonium-oxidizing bacteria (AnAOB) which has been used to estimate the anammox activity roughly. However, the quantitative relationship between the color and activity of anammox sludge still remains unknown. In this study, the chromaticity, activity and their correlation were systematically investigated at different steady-state nitrogen loading rates. The chromaticity of anammox sludge was digitalized by the CIE L*a*b* color space. The results revealed that the average chroma value was found to be significantly correlated with specific anammox activity ( r = 0.940, p < 0.01) and the cluster centers of chromaticity coordinates (a*, b*) of anammox sludge were established to define the typical working states of anammox system. The visible spectra of anammox sludge were proved to originate from the cytochrome c. The correlation between chroma and heme c concentration of anammox sludge was consistent with the fully-reduced cytochrome c and the chroma was determined by both content and redox ratio of cytochrome c. The chromaticity of anammox sludge was able to be linked with the anammox activity via reduced cytochrome c content. The gene abundance of cytochrome c synthetase linked the chromaticity with AnAOB quantity via total cytochrome c content, while the enzyme activity of octaheme hydrazine dehydrogenase linked the chromaticity with AnAOB activity via reduced cytochrome c ratio. Moreover, the redundancy analysis proved that heme c, as the key component of cytochrome c, was the most important explanatory variable accounting for the maximum 69.6% of the total variation of the anammox community, which correlated positively with the relative abundance of dominant AnAOB ( Candidatus Kuenenia). This work aimed at demonstrating the chromaticity of anammox sludge could be developed as an alternative intuitive anammox activity indicator which will promote the monitoring and optimization of anammox process.
研究领域:
环境工程
·
微生物学
·
生物技术
✓ 已验证
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2024
·
IF 9.7
Yu Feng,Xingyu Zhang,Caiwei Zhang,Haozhe Xu,Xiaoming Ji,Jianfang Wang,Peng Wu,Feiyue Qian,Chongjun Chen,Yaoliang Shen,Wenru Liu
In this study, waste iron scraps (WIS) were exerted to alleviate sulfide inhibition on anammox bacteria and promote anammox nitrogen removal from sulfide-containing wastewater. Short-term batch experiments showed that WIS-addition led to the anammox bacteria activity increasing by 124.8 % at an initial sulfide concentration of 40 mgS/L. During the long-term experiments, the nitrogen removal rate (NRR) reached to 8.76 kg/(m 3 ·d) in the WIS-added reactor, while the maximum NRR was only 3.77 ± 0.31 kg/(m 3 ·d) in the non-WIS reactor. In contrast to anammox bacteria development in the non-WIS reactor, the relative abundance of Candidatus Kuenenia (1.4–3.7 %) declined significantly in the WIS-added reactor, but novel potential anammox bacteria Brocadiaceae_unclassified (60.1 %-78.6 %) were highly enriched. Overall, the experimental evidence suggested that WIS-addition not only mitigated the sulfide inhibition on anammox bacteria, but also promoted novel anammox bacteria proliferation. The findings of this work provide a promising solution for wide engineering applications of anammox treating sulfide-containing wastewater.
研究领域:
环境工程
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2026
·
IF 10.6
Wentao Wang,Weiyi Xiong,Qing Jiang,Long-jun Ding,Ping Wang,Jiang-Shan Li,Hui Yin,Peng Cai
Anaerobic ammonium oxidation is a critical pathway for nitrogen (N) loss in terrestrial environments, and its coupled interactions with As(V) reduction has recently been recognized. While Fe-bearing minerals are suspected to regulate this coupling, the underlying mechanisms remain elusive. This study demonstrates that Fe-bearing minerals (ferrihydrite, goethite, montmorillonite, illite, and kaolinite) actively mediate anammox-coupled As(V) reduction by facilitating electron transfer and promoting Fe redox cycling. The presence of these minerals increases As(V) reduction by 1.2–2.4-fold and NH 4 + removal by 1.65–3.15-fold, with ferrihydrite exhibiting the strongest effect. In addition to accelerating reaction rates, these minerals reshape N partitioning and microbial community structure: clay minerals primarily influence early-stage NH 4 + dynamics via adsorption, whereas Fe (hydr)oxides sustain long-term coupling by suppressing nitrite oxidizers, limiting NO 3 - accumulation, enriching anammox and Fe-reducing bacteria, and enhancing microbial network stability. Redox-equivalent analysis further shows that these minerals increase the electron utilization efficiency of NH 4 + oxidation coupled with Fe(III) and/or As(V) reduction from 17% to 19–75%. Furthermore, As(V) reduction rates correlate strongly with the mineral specific surface area and functional genes including hzsA , omcS , arsC and arrA . This highlights the combined roles of surface-mediated electron transfer, enhanced Fe(III)/Fe(II) cycling, and coordinated regulation of N transformation and respiratory As(V) reduction. These findings identify Fe-bearing minerals as electron-buffering and -channeling phases that organize interfacial electron flow and strengthen N-Fe-As redox couples, providing a mechanistic framework for mineral-driven biogeochemical interactions in As-contaminated soils and sediments.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2024
·
IF 8
Xun Weng,Meng-Jiao Gao,Zheng Mao,Hui-Min Fu,Shi-Jun Li,Peng Yan,You-Peng Chen
Iron nanoparticles (FeNPs) generated from industrial activities could end up into the sewer system, and potentially affect wastewater treatment processes. The impact of FeNPs on anammox process is getting increasing attention. However, the resistance mechanism of anammox granular sludge (AnGS) to FeNPs has not been fully elucidated. The current study investigated the metabolic and morphological response of AnGS to acute and chronic FeNPs exposure. Results showed that nitrogen removal efficiencies were elevated at 1–4 mM FeNPs concentrations compared to 0–0.5 mM FeNPs. Extracellular protein and tyrosine-like and tryptophan-like fluorophore secretions of AnGS were stimulated by FeNPs, which largely contributed to the adsorption of FeNPs on AnGS surface. FeNPs exposure triggered higher necrotic fraction of AnGS compared with no FeNPs condition. Highly absorbed particles appeared inside the bacterial cells of AnGS, soft X-ray imaging illustrated that anammox bacteria maintained intact cellular and anammoxosome structures whereas non-anammox bacterial structures were damaged under FeNPs exposure. Anammox bacterial abundance increased from 4.84% to 20.64%, when FeNPs concentrations increased from 0 mM to 4 mM, and anammoxosome membrane ensured anammox bacterial metabolism under FeNPs exposure. This study extended fundamental understanding of AnGS resistance mechanisms to FeNPs.
研究领域:
环境科学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2017
·
IF 5.651
Zheng-Zhe Zhang,Hai-Yan Hu,Jia-Jia Xu,Zhi-Jian Shi,Yang-Yang Shen,Man-Ling Shi,Ren-Cun Jin
The increasing use of engineered nanoparticles (NPs) poses an emerging challenge to biological wastewater treatment. The long-term impact of CuNPs on anaerobic ammonium oxidation (anammox) process was firstly investigated in this study. The nitrogen removal capacity of anammox reactor was nearly deprived within 30 days under the stress of 5.0 mg L −1 CuNPs and the relative abundance of anammox bacteria (Ca. Kuenenia ) was decreased from 29.59% to 17.53%. Meanwhile, copper resistance genes associated with the Cus, Cop and Pco systems were enriched to eliminate excess intracellular copper. After the withdrawal of CuNPs from the influent, the nitrogen removal capacity of anammox biomass recovered completely within 70 days. Overall, anammox biomass showed susceptibility, resistance and resilience to the stress of CuNPs. Therefore, the potential impacts of ENPs on anammox-based processes should be of great concern.
研究领域:
环境科学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2022
·Vol. 29
·期 25
·37266-37276
·
IF 5.19
Zhang Li,Lan Shuang,Dou Quanhao,Hao Shiwei,Wang Yueping,Peng Yongzhen
The zero-valent iron-anaerobic ammonium oxidation (ZVI-anammox) system has received widespread attention due to its excellent nitrogen removal performance and user-friendly operation. However, its disadvantages include a short service life, high ZVI consumption, and poor system stability. The use of ultrasound as a physical method is increasing in various water treatment processes. In this study, a series of batch tests were conducted to obtain the best ultrasonic parameter and explore the comprehensive effects of ultrasound on a ZVI-anammox system. The highest specific anammox activity of the ZVI-anammox system was found to be 2.88 mg total nitrogen/g of volatile suspended solids/h after ultrasonic treatment (0.2 w/mL, 5 min), which was 37.85% higher than a control group. Additionally, the service life of ZVI extended by 28.57% and the total nitrogen removal efficiency changed from 58.03–72.08 to 63.92–78.33% under ultrasonic irradiation. These phenomena were related to the mechanical force and cavitation of ultrasonic waves. Judging from the characteristics of sludge and ZVI, ultrasound can promote anammox sludge granulation, ease ZVI passivation, and enhance the stability of the entire system. This paper also briefly discusses the impact mechanisms of ultrasound on the ZVI-anammox system. In brief, ultrasound destroys the surface structure of ZVI and thus provides numerous attachment points for microorganisms that improve the performance of the entire system. The proposed ultrasound combined with ZVI is a novel method that has potential for use in large-scale engineering applications in actual sewage treatment after comprehensive analysis.
研究领域:
环境工程
·
水处理技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）