COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Qingxia Zhou,Yanrong Cheng,Xia Guo
Developing a simple and effective method for detecting and separating amino acids has practical significance. Aqueous two-phase system (ATPS) has the advantages of mild preparation conditions, good stability, and high biocompatibility. In this study, an ATPS exhibiting distinct responses and selectivity profiles toward Phe, Trp, and Tyr was constructed using (1 S , 2 S )-(-)-1,2-diphenylethylenediamine (DPED), sodium dodecyl sulfate (SDS), and sodium carbonate (Na 2 CO 3 ). It is found that the amino acids can trigger the transformation of the DPED/SDS/Na 2 CO 3 ATPS into a single-phase system, but the critical concentrations required for this phase transition vary significantly among the amino acids: Phe and Trp induce the transition at very low concentrations, whereas Tyr requires a much higher concentration (8–13 times greater than Phe and Trp under optimal conditions). This stark contrast enables the DPED/SDS/Na 2 CO 3 ATPS to serve as a sensitive and visually distinguishable platform for differentiating Tyr from Phe and Trp. Moreover, the DPED/SDS/Na 2 CO 3 ATPS exhibits selective phase partitioning: Phe and Trp preferentially concentrate in the top phase, while Tyr predominantly distributes into the bottom phase. This distinct partitioning behavior allows for the efficient separation of Phe and Trp from Tyr, highlighting the system’s dual functionality for both detection and purification of Tyr from Phe and Trp. These findings indicate the potential of using this system to develop methods for separating and detecting the amino acids.
研究领域:
分析化学
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生物化学
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分离科学
·
材料科学
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产品关联
DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 29
·期 17
·25810-25823
·
IF 4.223
Shang Zhijie,Xu Pan,Yue Haoyu,Feng Dongyue,Zhu Taohua,Li Xinxue
The increasing diesel pollution accidents pose a serious threat to the ecological environment and human health. Remediation of diesel-contaminated soil (DCS) has attracted widespread attention during the past few decades. This work proposed an approach for the remediation of DCS by alkoxyethanol aqueous two-phase extraction (ATPE), which was an application of this small molecule aqueous two-phase system (ATPS). In addition, the influence of temperature, stirring speed, stirring time, and solid–liquid ratio on the removal of diesel was explored respectively. The removal efficiency of diesel could reach more than 97.18% in 18 min. Meanwhile, ATPS had high reusability, and the removal efficiency remained above 85.17% in the reuse process. Alkoxyethanol ATPE could effectively remove diesel hydrocarbons with different carbon chain lengths and the remediation process hardly caused residual organic solvents on the soil surface according to the analysis of gas chromatography-mass spectrometry (GC–MS) and Fourier transforms infrared (FT-IR), which could be regarded as the distinct advantage compared to the traditional surfactant washing method and organic solvent extraction method. The study of soil physicochemical properties and wheat germination proved that the soil structure and properties changed little after ATPE remediation. And finally, the mechanism of alkoxyethanol ATPE was intensively discussed according to the remediation characteristic. This work provided an efficient method for the remediation of DCS and widened the application fields of alkoxyethanol ATPS as well. Graphical abstract
研究领域:
环境科学
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土壤学
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化学工程
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污染控制
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产品关联
DOI
PubMed
高影响因子
Soft Matter
·
2018
·Vol. 14
·期 9
·1552-1558
·
IF 3.709
Qingming Ma,Hao Yuan,Yang Song,Youchuang Chao,Sze Yi Mak,Ho Cheung Shum
Partitioning refers to the distribution of solute molecules in the two immiscible phases of a mixture of two solutions, such as an aqueous two-phase system (ATPS). The partitioning of RNA and peptide has been adjusted in situ to facilitate their assembly into intracellular membraneless organelles. Despite the immense potential of this approach in artificial systems, a partitioning-dependent assembly of macromolecules has been limited, due to the sophisticated processing associated with their in situ modification. Here we demonstrate an approach to direct the assembly of polyelectrolytes in an ATPS through varying their partitioning via pH changes. Microcapsules can be converted to microgel particles as the polyelectrolytes selectively partition to different emulsion phases when changing pH. Such partitioning-dependence can also be equally applied for complexing hydrophilic nanoparticles with polyelectrolytes in an ATPS. By enabling access of hydrophilic materials across the aqueous interface freely, the ATPS allows modification of their intrinsic properties in situ; this advantage will inspire more versatile control over the partitioning of hydrophilic materials and will create new multi-functional biomaterials.
研究领域:
生物化学
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材料科学
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分子生物学
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DOI
PubMed
高影响因子
ANALYST
·
2013
·Vol. 138
·期 21
·6445-6453
·
IF 3.969
Xiao Lin,Yuzhi Wang,Qun Zeng,Xueqin Ding,Jing Chen
A satisfactory protocol of protein extraction and separation has been established based on the ionic liquid aqueous two-phase system (IL-ATPS) for the purification of bioactive substances. Compared with the effects of eight different ionic liquids, 1-octyl-3-methylimidazolium bromide ([omim][Br]) was selected as the suitable ionic liquid. Based on the single-factor experiment, an initial serial investigative test was used to identify the optimal conditions of the extraction. Owing to their different isoelectric points, bovine serum albumin (BSA), hemoglobin (Hb) and lysozyme (Lys) were used to determine the effect of pH value on the protein extraction. Trypsin (Try) was used to confirm the protein activity. The linearity for analyzing BSA, Hb, Try and Lys was in the concentration range of 0.05–1.00 mg ml−1, 0.025–1 mg ml−1, 0.01–1.00 mg ml−1 and 0.01–1.00 mg ml−1, respectively, with a correlation coefficient of between 0.9985 and 0.9999. Limits of detection (LODs) were 16.47–7.02 μg ml−1 and RSDs of inter-day stability were less than 2.9%. Repeatability and precision were respectively lower than 5.3% and 1.1%. Under the optimum conditions, the average recoveries of BSA, Hb, Try and Lys were 90.5%, 94.5%, 92.7% and 93.8% and the obtained RSDs were 1.19%, 1.23%, 1.34% and 1.04%, respectively. According to UV spectra, conductivity, dynamic light scattering (DLS), and transmission electron microscope (TEM) images, the cluster phenomenon originating from IL itself or combined with protein was evaluated. As the driving forces which are involved in the partitioning of protein between the IL-rich phase and the phosphate phase, the cluster phenomenon could, in principle, be applied to a variety of different samples and exhibited potential value.
研究领域:
生物化学
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分离科学
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产品关联
DOI
PubMed
高影响因子
PHARMACEUTICAL CHEMISTRY JOURNAL
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2017
·Vol. 51
·期 6
·491-493
·
IF 0.445
Zhu Jian,Jin Zhi-Min
Insoluble camptothecin was converted into the corresponding hydrosoluble analog, camptothecin carboxylate, by using 1,1,3,3-tetramethylguanidine as hydrolyzing agent. The ethanol/K 2 CO 3 /H 2 O aqueous two-phase system was established to extract the hydrosoluble camptothecin carboxylate. Through two-stage extraction from crude camptothecin of 5.4%, purity, final hydrosoluble camptothecin was obtained with a purity of 98.5% at a total yield of 78.4%.
研究领域:
药物化学
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分离工程
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产品关联
DOI
高影响因子
LAB ON A CHIP
·
2017
·Vol. 17
·期 19
·3310-3317
·
IF 6.045
Chunmei Zhou,Pingan Zhu,Ye Tian,Xin Tang,Rui Shi,Liqiu Wang
Existing approaches for droplet generation with an ultra-low interfacial tension using aqueous two-phase systems, ATPS, are either constricted by a narrow range of flow conditions using passive methods or subjected to complex chip fabrication with the integration of external components using active actuation. To address these issues, we present a simple approach to produce uniform ATPS droplets facilitated by oil-droplet choppers in microfluidics. Our solution counts on the synchronized formation of high-interfacial-tension oil-in-water and low-interfacial-tension water-in-water droplets, where the ATPS interface is distorted by oil droplets and decays into water-in-water droplets. In the synchronization regime, the size and generation frequency of ATPS droplets can be controlled independently by tuning the flow rates of the dispersed aqueous and oil phases, respectively. Our method demonstrates high uniformity of droplets (coefficient of variation between 0.75% and 2.45%), a wide range of available droplet size (droplet radius from 5 μm to 180 μm), and a maximum generation frequency of about 2.1 kHz that is nearly two orders of magnitude faster than that in existing methods. We develop theoretical models to precisely predict the minimum and maximum frequencies of droplet generation and the droplet size. The produced ATPS droplets and oil choppers are separated in the channel using density difference. Our method would boost emulsion-based biological applications such as cell encapsulation, biomolecule delivery, bioreactors, and biomaterials synthesis with ATPS droplets.
研究领域:
生物医学工程
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微流控技术
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生物材料合成
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细胞生物学
·
药物递送系统
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DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2025
·
IF 2.1
Qingxia Zhou,Yanrong Cheng,Delan Ge,Xia Guo
PEG/salt aqueous two-phase systems (ATPSs) are the most common ATPSs because they are nontoxic, biocompatible, and inexpensive; however, the limited polarity difference between the two phases hinders their application. Although increasing the molecular weight of PEG can increase the polarity difference between the two phases, the significant viscosity of the PEG phase makes mass transfer difficult once the PEG molecular weight is too high. In the present study, we focus on enhancing the performance of low-molecular-weight PEG (PEG 400)/salt ATPS by using different ionic surfactants as adjuvants; the surfactants include anionic surfactants sodium dodecyl sulfate and sodium dodecyl benzenesulfonate, cationic surfactant dodecyl trimethylammonium bromide, and zwitterionic surfactant N-dodecyl-N, N-dimethyl-3-ammonio-1-propanesulfonate. It is found that all of these surfactants can promote the PEG 400/salt ATPS formation, but their effects on the ATPS extraction performance are different. By using tryptophan and vanillin as model extracts, it is found that the anionic and zwitterionic surfactants significantly enhance the extraction performance of PEG 400/salt ATPS, while the cationic surfactant significantly reduces the ATPS extraction performance. These results illustrate the potential of anionic and zwitterionic surfactants as adjuvants in enhancing the performance of low-molecular-weight PEG/salt ATPS.
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产品关联
DOI
高影响因子
LANGMUIR
·
2024
·
IF 3.7
Chunyang Liu,Yanrong Cheng,Delan Ge,Ju Xie,Xia Guo
Aqueous two-phase systems (ATPS) are considered a new type of green separation system, but their low selectivity to target molecules limits their practical application. In this study, we used surfactant@MWCNT as an adjuvant to improve the selectivity of PEG/(NH4)2SO4 ATPS. The surfactants used include Triton X-100 (TX-100), Tween 80, sodium dodecyl sulfate (SDS), sodium dodecyl benzenesulfonate (SDBS), and bis(2-ethylhexyl)sulfosuccinate sodium salt (AOT). Nine aromatic compounds, including syringic acid, vanillin, tryptophan, phenylalanine, tyrosine, mandelic acid, theophylline, theobromine, and cephalexin, were selected as model extracts. The results showed that the use of the surfactant@MWCNT composite as an adjuvant significantly improved the selectivity of the ATPS toward syringic acid among the nine extracts. Moreover, the selectivity of the ATPS for syringic acid with the TX-100/MWCNT composite as the adjuvant is better than that with the Tween 80/MWCNT composite as the adjuvant, followed by that with the anionic surfactant/MWCNT composite as the adjuvant. The enhanced selectivity of ATPS by the surfactant/MWCNT composite is ascribed to the spatial effect and the electrostatic and hydrophobic interactions between the extract and the surfactant adsorbed on the MWCNT.
研究领域:
化学工程
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分离科学
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纳米技术
·
环境科学
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产品关联
DOI
PubMed
高影响因子
Separations
·
2021
·Vol. 8
·期 11
·212
·
IF 2.777
Bin Jiang,Shaojing Zhong,Hongliang Yu,Peifeng Chen,Baoyun Li,Dongmei Li,Chunhong Liu,Zhibiao Feng,Bo Tian
Thiocyanate could effectively inhibit bacteria in milk and extend the shelf life of milk. However, excessive addition will lead to health risks. Therefore, the determination of thiocyanate in raw milk has received a lot of attention, but the determination could be interfered with by other components in raw milk and the pre-treatment of raw milk is complex. In this study, a new pretreatment method combined with ion chromatography (IC) for rapid and sensitive determination of thiocyanate is proposed. An acetonitrile/(NH4)2SO4aqueous two-phase system (ATPS) was developed for the separation and enrichment of thiocyanate in raw milk. Response surface methodology was performed to optimize the extraction conditions and an efficient pretreatment were obtained using ATPS composed of 42% acetonitrile (w/w) and 16% (NH4)2SO4(w/w), with the pH 4.7, and the recovery of thiocyanate reached 107.24 ± 0.5%, and the enrichment ratio was 10.74 ± 0.03. IC was used to establish a thiocyanate enrichment method. The linear range was from 0.05 to 15 mg/L and R2= 0.998, the limit of detection (LOD) was 0.2 μg/L, the limit of quantification (LQD) was 0.6 μg/L. Hence, it is feasible to combine ATPS with IC for the enrichment and determination of thiocyanate in raw milk.Keywords:thiocyanate;raw milk;aqueous two-phase system;ion chromatography;extraction
研究领域:
食品科学
·
分析化学
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产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2014
·
IF 3.065
Yingchun Wu,Yun Wang,Wenli Zhang,Juan Han,Yan Liu,Yutao Hu,Liang Ni
Aqueous two-phase extraction (ATPE) was employed for the extraction and preliminary purification of anthocyanins in grape juice (GA). The influences of such factors as the type and concentration of salt, the concentration of alcohol and the quantity of grape juice, extraction time, system pH and temperature were investigated to obtain the optimal extraction conditions. They were listed as follow: sodium dihydrogen phosphate (NaH 2 PO 4 ) concentration 28% (w/w), ethanol concentration 25% (w/w), the quantity of grape juice 1 mL, temperature 298.15 K, time 1 h and no pH adjustment. Under the optimal conditions, the relative and absolute recovery of GA in the top phase reached 99.35% and 99.26% in one step, respectively, while the sugar-removing rate reached 75.08%. The results of Multistage and scale-up extractions showed that it could remain over 90% GA in the top phase and remove more than 90% sugars after continuous extraction for two times. Scale-up extractions and the GA stability results showed the effectiveness and great potential of this method in processing mass grape juice.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
Advanced Materials Technologies
·
2020
·Vol. 5
·期 6
·2000045
·
IF 5.969
Hui Wang,Haitao Liu,Fan He,Wenwen Chen,Xu Zhang,Mengqian Zhao,Li Wang,Jianhua Qin
Hydrogel capsules are widely applied in materials science, pharmaceutical science, and tissue engineering due to their tunable nature, defined core–shell architecture, and proper biocompatibility. Specially, multi-core capsules are highly functional carriers with notable advantages for co-encapsulation of diverse incompatible components without cross-contamination. However, current approaches for generating such capsules are limited by using gas/oil-water-oil systems, leading to poor biocompatibility and functions due to the gas/oil cores. Herein, a simple and new approach is proposed for flexible fabrication of multi-aqueous core hydrogel capsules (MACHC) using microfluidic aqueous two-phase system (µATPS). In this system, a flow-focusing microfluidic device is designed to create a stable laminar flow with two immiscible liquids composed of polyethylene glycol and dextran, which is sheared by oil phase to further effectively produce MACHC. The volume and components of each core can be well tailored by precisely adjusting the distinct flows, while the number of cores is determined by the orifice design of microfluidic chip. The MACHC are successfully used to co-encapsulate heterogeneous hepatic and endothelial cells, indicating good biocompatibility and multi-functionality of the multi-core carriers. The proposed µATPS-based approach is stable and convenient, holding promise for generation of multi-responsive materials, tissue engineering, and co-delivery of diverse drugs.
研究领域:
材料科学
·
组织工程
·
药学
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2013
·
IF 2.487
Qiang Wu,Dong-Qiang Lin,Shan-Jing Yao
The aqueous two-phase system (ATPS) with polyethylene glycol (PEG) and hydroxypropyl starch (HPS) was evaluated for the extraction of human immunoglobulin G (IgG) from human serum albumin (HSA). The partitions of IgG in PEG/sulphate, PEG/phosphate, PEG/Dextran and PEG/HPS ATPSs were compared, and the results indicated that PEG/HPS system was most suitable for IgG extraction. The effects of the concentrations of PEG, HPS and NaCl addition and pH on the partition of IgG and HSA in PEG/HPS ATPS were investigated. It was found that with 15% NaCl addition at pH 8.0 IgG could be largely recovered in the top PEG-rich phase and most of HSA kept in the bottom phase. In addition, the response surface methodology (RSM) was used to optimize the extraction of IgG from the protein mixture of IgG and HSA. The optimal conditions were obtained as 12% (w/w) PEG 4000, 18% (w/w) HPS and 10% (w/w) NaCl at pH 8.0. The extraction yield of IgG in the top phase was 99.2% and the purification factor could reach 5.28. The back extractions of IgG into a phosphate-rich bottom phase were also studied. The total purification factor was 5.73 with the yield of 84.0%. The results indicated that PEG/HPS ATPS might be a promising alternative for the primary recovery of IgG from the complicated feedstock.
研究领域:
生物化学
·
蛋白质工程
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DOI
高影响因子
危险品化学品经营许可证（带存储）