JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2024
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IF 2.8
Feng Xu,Jiayong Yu,Yinliang Wu
An integrated method combining solid-phase extraction (SPE) with ultra-performance liquid tandem mass spectrometry (UPLC-MS/MS) has been established for quantifying bacitracin (BTC), bacitracin zinc (BZ), and bacitracin methylene disalicylate (BMD) in animal feed. A pretreatment procedure that can effectively, quickly, and simultaneously extract and purify BTC, BZ, or BMD in feed was developed for the first time through the optimization of extraction and SPE conditions. After extraction with acetonitrile + methanol + 15 % ammonia solution (1:1:1, v : v : v ) and dilution with EDTA solution (1.5 mmol/L, pH 7.0), a SPE procedure was carried out with C 18 cartridge. Following LC-MS/MS analysis utilized a Waters Peptide BEH C 18 column with a gradient elution of 0.1 % formic acid in water/acetonitrile with. This method demonstrated a strong linear correlation (R 2 > 0.9980) across a 0.01–1.0 mg/L concentration span, based on a matrix-matched standard curve. Satisfactory recoveries of BTC (bacitracin A, B 1 , B 2 , and B 3 ), BZ, and BMD in different feeds were obtained from 80.7 % to 108.4 %, with relative standard deviations below 15.7 %. Low limits of quantification ranging within 7.2–20 μg/kg were achieved for bacitracin A , B 1 , B 2 , and B 3 . This method provided an effective and reliable detection method to prevent the addition of BTC and different BTC formulations in feeds.
研究领域:
分析化学
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兽医学
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食品安全
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PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE
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2024
·Vol. 59
·期 24
·10987-10997
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IF 3.5
Ao Haiyong,Duan Keke,Zhang Dongxue,Xun Xiaowei,Feng Fangfang,Liu Huiwen,Li Jiaxin,Li Yaqiang,Wan Yizao
Bacterial cellulose (BC) with excellent flexibility and biocompatibility has aroused great attention for wound healing. However, the surface inertness of BC nanofibers tremendously hindered its biofunctionalized to improve the antibacterial ability and cytocompatibility. Herein, the biofunctionalized BC was fabricated by in situ growing a uniformly polydopamine (PDA)/polyethyleneimine (PEI) coating and grafting bacitracin (BA) on the coating (BA-PPBC). The results confirmed that the BA-grafted PDA/PEI coating was distributed uniformly on the surface of BC nanofibers, and the natural three-dimensional pore structure and other physical properties of pristine BC were retained. Compared to BC, the obtained BA-PPBC materials exhibited excellent antibacterial activities against the adhesion and colonization of S. aureus and MRSA . Moreover, the BA-PPBC not only had no obvious cytotoxicity to NIH-3T3 cells, but also improved the adhesion, proliferation, and spread out of cells. This work provides a biofunctionalized BC dressing to simultaneously meet the requirements of wound healing.
研究领域:
生物材料学
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医学工程
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伤口愈合研究
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高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2016
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IF 3.902
Bin’en Nie,Haiyong Ao,Teng Long,Jianliang Zhou,Tingting Tang,Bing Yue
Bacitracin immobilized on the titanium (Ti) surface significantly improves anti-bacterial activity and biocompatibility in vitro . In the current study, we investigated the biologic performance (bactericidal effect and bone-implant integration) of bacitracin-modified Ti in vivo . A rat osteomyelitis model with femoral medullary cavity placement of Ti rods was employed to analyze the prophylactic effect of bacitracin-modified Ti (Ti-BC). Thirty-six female Sprague Dawley (SD) rats were used to establish the Ti implant-associated infection. The Ti and Ti-BC rods were incubated with and without Staphylococcus aureus to mimic the contaminated Ti rod and were implanted into the medullary cavity of the left femur, and sterile Ti rods were used as the blank control. After 3 weeks, the bone pathology was evaluated using X-ray and micro-computed tomography (micro-CT) analysis. For the investigation of the Ti-BC implant osseointegration in vivo , fifteen SD rats were divided into three groups (N = 5), namely Ti, Ti-dopamine immobilized (Ti-DOPA), and Ti-BC. Ti rods were implanted into the left femoral cavity and micro-CT and histological evaluation was conducted after 12 weeks. The in vivo study indicated that Ti-immobilized bacitracin owned the prophylaxis potential for the infection associated with the Ti implants and allowed for the osseointegration. Thus, the multiple biofunctionalized Ti implants could be realized via immobilization of bacitracin, making them promising candidates for preventing the Ti implant-associated infections while retaining the osseointegration effects.
研究领域:
生物材料学
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医学工程
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微生物学
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DOI
PubMed
高影响因子
ACS Applied Nano Materials
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2022
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IF 6.14
Jingchao Wang,Siyi Li,Kunlong Yang,Tianyong Zhang,Shuang Jiang,Xianggao Li,Bin Li
Coupling heterojunction is an effective and feasible approach to suppressing the electron–hole recombination of a photocatalyst. However, the heterojunction performance is limited by poor interface contact between two semiconductors. Therefore, an in situ partial conversion procedure was proposed to fabricate the flower-spherical BiOI/Bi5O7I (BBOI) heterojunction using bacitracin as a template. The introduction of bacitracin can regulate the assembly of nanosheets into a flower-spherical structure, which caused the BBOI photocatalysts to have larger specific surface areas than BiOI or Bi5O7I. In particular, the dosage of bacitracin can control the thermal conversion of BiOI to Bi5O7I. The in situ partial conversion of BiOI caused the formation of an intimate S-scheme heterojunction interface between BiOI and Bi5O7I, which efficiently inhibited the combination of photogenerated carriers. Upon visible-light irradiation, BBOI-3 exhibited the highest catalytic ability for the simultaneous reduction of Cr(VI) (100%) and oxidative degradation of tetracycline hydrochloride (TH) (80.0%). This work provides an opinion to construct the high-performance heterojunction photocatalysts to environmental remediation.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
Food Analytical Methods
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2025
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IF 3
Na Guanqiong,Chen Ping,Qian Yanya,Meng Danqun,Liu Zhipeng,Shan Yujuan
Traditional fluorescent immunosensors usually suffer from high background signals in food samples, which significantly reduces their detection sensitivity. In this study, δ-iron oxyhydroxide (δ-FeOOH) nanosheets with anti-bacitracin zinc (BAC) monoclonal antibodies (mAb) were conjugated as fluorescence receptors, while amino-functionalized carbon quantum dots (CQDs) as fluorescence donors for BAC. A novel fluorescence immunoassay was developed that eliminated background noise based on the “turn on” principle. Upon introduction of BAC into the sample, the nano-assembled probe showed an increase in fluorescence recovery, leading to higher fluorescence intensity at elevated BAC concentrations. This strategy exhibited a linear response for BAC concentrations ranging from 5 to 40 ng/mL, with a limit of detection (LOD) of 2.04 ng/mL. Our findings present a promising immunological approach for the sensitive and selective detection of BAC residues in food.
研究领域:
分析化学
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食品科学
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生物医学工程
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DOI
高影响因子
ACS Applied Materials & Interfaces
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2019
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IF 8.456
Shanshan Wang,Yingyi Wang,Yi Peng,Xiaoming Yang
Integrating kinds of bactericides as one entirety shows prospect for improving antibacterial efficiency, thus a new type of nanoclusters based on bactericide-directed herein emerged as a combination for achieving a higher antibacterial effect. Specifically, an easily operated approach for preparing bacitracin-directed silver, gold, and copper nanoclusters (AgNCs, AuNCs, and CuNCs, respectively) was proposed. Meanwhile, AgNCs@Bacitracin, AuNCs@Bacitracin, and CuNCs@Bacitracin emitted cyan, yellow, and red colors of fluorescence, respectively, and different sizes and nitrogen contents of these nanoclusters played a critical role for their fluorescence alterations. Significantly, AgNCs@Bacitracin exhibited robust bacteria-killing efficiency than other nanoclusters, owing to its marked damage on the bacterial membrane. Additionally, propidium iodide (PI) staining indicated the bacterial membrane damage of 72.3% to the bacteria population followed by the treatment with AgNCs@Bacitracin, and the introduction of AgNCs@Bacitracin lead to the higher reactive oxygen species (ROS) production, facilitating their attractive antibacterial activity. Moreover, this strategy of developing new nanoclusters broadened the avenues for designing the improved antibacterial materials.
研究领域:
纳米技术
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材料科学
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微生物学
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DOI
PubMed
高影响因子
Analytical Methods
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2021
·Vol. 13
·期 25
·2804-2811
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IF 2.896
Feng Qin,Shujuan Wang,Mingxia Gao,Xiangmin Zhang
The highly effective detection of pathogens in complex biological samples is an attractive and critical topic of study. Bacitracin is a novel broad-spectrum antimicrobial peptide to enrich bacteria via interactions with the membrane surface of the different bacterial cells. In this study, for the first time, bacitracin was immobilized on the surface of Fe3O4@PDA magnetic nanoparticles for the enrichment of Staphylococcus aureus (G+) and Klebsiella pneumoniae (G−). Combined with matrix-assisted laser desorption ionization time-of-flight mass spectrometry, a rapid and sensitive detection method for these two bacteria was developed. In this method, the detectable concentration of bacteria was decreased by 2–3 orders of magnitude in unenriched samples. The enrichment and identification can be completed in one hour. All these results demonstrated that the bacitracin-functionalized magnetic composite has potential for use in the large-scale enrichment and isolation of target pathogens from complex biological samples, opening a new avenue for the rapid and sensitive detection of pathogens.
研究领域:
微生物学
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分析化学
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医学检测
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DOI
PubMed
高影响因子
Microbiology Spectrum
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2023
·Vol. 11
·期 6
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IF 3.7
Yuhua Gu,Shuaijie Song,Qingrui Zhu,Ruilin Jiao,Xu'ai Lin,Fan Yang,Stijn van der Veen
Neisseria gonorrhoeae is a multidrug-resistant bacterial pathogen for which ceftriaxone mono-antimicrobial therapy and ceftriaxone plus azithromycin dual-antimicrobial therapy are the only remaining effective therapies. However, ceftriaxone efficacy is threatened by the global dissemination of the high-level ceftriaxone-resistant FC428 clone, while azithromycin has in recent years been removed from the recommended dual therapy in some countries as a result of increasing (high-level) azithromycin resistance. Inclusion of an alternative second antimicrobial could be an effective strategy to protect ceftriaxone from (further) resistance development. In this study, we investigated gonococcal susceptibility to bacitracin and determined its synergistic anti-gonococcal activity with ceftriaxone. Gonococcal susceptibility to bacitracin was investigated for 449 contemporary clinical isolates using the agar dilution method. Bacitracin displayed consistent activity against the N. gonorrhoeae strain collection, with a minimum inhibitory concentration (MIC) range of 2–32 mg/L and a MIC90 of 32 mg/L. Furthermore, bacitracin was bactericidal in time-kill assays, particularly against the tested FC428-associated isolate, which showed 105-fold inactivation after 8 hours of exposure to a 4× MIC dose. Importantly, bacitracin and ceftriaxone displayed synergistic activity, with a fractional inhibitory concentration index ≤0.5 for the majority of tested strains. Finally, spot assays showed strong synergistic activity against the FC428-associated isolates, with up to 200-fold reduced plating efficacy for bacitracin combined with ceftriaxone compared with ceftriaxone only. In conclusion, bacitracin shows synergistic anti-gonococcal activity with ceftriaxone and might therefore be an interesting candidate for inclusion in a dual-antimicrobial therapy with ceftriaxone.
研究领域:
微生物学
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传染病学
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抗生素耐药性
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临床微生物学
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DOI
高影响因子
Materials Horizons
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2026
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IF 11.4
Ziqi Wang,Hongyi Lv,Juan Zhang,Tianyue Zhang,Yishan Wang,Wanting Yuan,Yijing Zhang,Yuedong He,Lijuan Zhao,Zhongyi Zhu,Jinrong Wu,Yi Wang
Injectable hydrogels are attractive for minimally invasive soft-tissue repair, yet most systems undergo post-gelation swelling in aqueous environments, leading to dimensional instability and progressive mechanical deterioration. Here, we report a bacitracin-regulated poly(2-hydroxyethyl methacrylate) (PHEMA) hydrogel that exhibits in situ gelation together with intrinsic mild negative swelling under hydrated conditions. In this system, bacitracin serves as a multifunctional molecular regulator that not only accelerates gel formation under mild conditions but also modulates postgelation network evolution through multivalent hydrogen bonding and hydrophobic associations with PHEMA chains. As a result, the hydrogel undergoes a distinct hydration-driven network evolution rather than conventional swelling expansion, leading to spontaneous but mild volumetric contraction and improved structural stability in aqueous environments. Notably, this negative swelling behavior occurs directly under physiologically relevant hydrated conditions and without a pronounced overswelling stage. The resulting hydrogel displays enhanced mechanical robustness, resistance to water-induced structural degradation, and concentration-dependent antibacterial activity. By further incorporating estradiol-loaded mesoporous silica nanoparticles, the dimensionally stable network enables sustained bioactive release and promotes vaginal wound healing in vivo. More broadly, this work identifies bacitracinregulated hydration-driven network evolution as a route to controlling post-gelation volume behavior in injectable hydrogels and provides a design principle for soft-tissue repair in moist physiological environments.
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PubMed
高影响因子
Jove-Journal of Visualized Experiments
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2024
·
IF 1.2
Yushu Tang,Ning Hao
Bacillus licheniformis and bacitracin have a huge application market and value in the fields of medicine, chemistry, aquaculture, agricultural, and sideline products. Therefore, the selection of B. licheniformis with high production of bacitracin is of great importance. In this experimental protocol, Bacillus with a high yield of bacitracin was isolated, purified, and identified from the fresh feces of healthy pigs. The inhibitory effect of secondary metabolite bacitracin on Micrococcus luteus was also tested. Thin-layer chromatography and high-performance liquid chromatography were used for the qualitative and quantitative detection of bacitracin. The physiological and biochemical characteristics of B. licheniformis were determined by relevant kits. The phylogenetic relationships of B. licheniformis were determined and constructed using gene sequence detection. This protocol describes and introduces the standard isolation, purification, and identification process of B. licheniformis from animal fresh feces from multiple perspectives, providing a method for the large-scale utilization of B. licheniformis and bacitracin in factories.
研究领域:
微生物学
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药理学
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农业科学
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水产养殖
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生物技术
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Rui Bai,Wei Chen,Xudong Zhou,Junpeng Li,Yi He,Yong Xiao,Feng Zhao
Bacitracin is renowned for its broad-spectrum antimicrobial activity and low propensity to induce resistance. However, how to achieve efficient bacitracin production remains a significant challenge. In this study, we developed a Bacillus licheniformis -CdSe biohybrid system that operates under sunlight, employing dual regulatory strategies of "increasing incomes" and "cutting expenses" to achieve efficient bacitracin production. For the increasing incomes strategy: CdSe converted solar energy into photoelectrons, providing B. licheniformis with additional energy and accelerating intracellular energy metabolism; For the cutting expenses strategy: Light exposure induced a metabolic pathway rearrangement in B. licheniformis , suppressing the pathway of pulcherrimin synthesis, thereby conserving energy and redirecting resources toward bacitracin synthesis. By integrating these two regulatory strategies, the bacitracin yield exhibited a remarkable increase, achieving a cumulative concentration of 390.9 mg·L −1 within 24 h. Comprehensive analyses, including biochemistry, electrochemistry, proteomics, and transcriptomics, demonstrated that light optimizes energy allocation by suppressing metabolic byproducts in B. licheniformis , thereby enhancing selectivity for the target product. CdSe efficiently harvested solar energy, accelerating intracellular regeneration of ATP and NAD( P )H, and promoting the production of precursor amino acids, ultimately boosting bacitracin synthesis. This study provides insight into how to design microbe-semiconductor biohybrid systems for the sustainable synthesis of high-value chemicals.
研究领域:
微生物学
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生物技术
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光化学
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合成生物学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
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2021
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IF 4.821
Zuoling Zhang,Min Xia,Pengfei Huang,Bin Di,Mengxiang Su
New stationary-phase materials are of great interest for use in hydrophilic interaction liquid chromatography (HILIC), a relatively new technique capable of separating highly polar compounds. In this study, a new strategy was established to prepare a novel Sil-Bac stationary phase by bonding bacitracin to silanised silica particles using hexamethylene diisocyanate as a spacer arm. The obtained material was characterised by Fourier transform infrared spectroscopy, Brunauer–Emmett–Teller analysis, transmission electron microscopy (TEM), and elemental analysis. The network layers observed through TEM and change in specific surface area from 315.0820 to 169.6758 m 2 ·g −1 after modification reflected the addition of bacitracin. The chromatographic behaviour of the Sil-Bac column showed that it can be used for HILIC. The influence of the chromatographic conditions on substance retention was explored by varying the acetonitrile content, column temperature, buffer concentration, and pH of the mobile phase. Using cytosine, uracil, nicotinamide, and isonicotinic acid as probes, the Sil-Bac column was demonstrated to have acceptable repeatability and stability, with a relative standard deviation of the retention time in the ranges of 0.05–0.18% for intra-day precision ( n = 3) and 0.16–3.28% for inter-day precision ( n = 3). Finally, the stationary phase was applied in separating basic nucleosides, water-soluble vitamins, and amino acids. A variety of bases and nucleosides could be separated within 7 min in a mobile phase of ACN/ammonium acetate (pH 3.0) = 90/10 (v/v), T = 30 °C. The Sil-Bac column was also compared with three commercial columns, and the results indicated that the analytes retained on the Sil-Bac column showed better retention and selectivity from an overall view. This novel stationary phase has a good separation ability and selectivity in HILIC mode and provides new ideas for the separation of polar compounds.
研究领域:
分析化学
·
色谱技术
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DOI
高影响因子
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