Frontiers in Microbiology
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2025
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IF 4.5
Zhuocheng Yao,Yuhan Yang,Yanchun Gong,Shiyi Shi,Yunying Ge,Weiliang Zeng,Deyi Zhao,Jianming Cao,Tieli Zhou,Mo Shen
Disinfectants are commonly utilized by humans to combat microorganisms. However, residual disinfectants may promote environmental antimicrobial resistance by facilitating horizontal gene transfer (HGT) of antibiotic resistance genes. Bronopol is a routinely used disinfectant that persists in the environment, and previous studies have concentrated on its ecotoxicity rather than its implications on the propagation of resistance genes. This study aimed to establish an in vitro conjugation model to investigate whether bronopol promotes the transfer of antibiotic resistance genes (ARGs) via plasmid conjugation. Using Escherichia coli DH5α and DC8855 as donors harboring RP4-7 and blaNDM-4-positive IncFII(K) plasmids, respectively, and J53 as the recipient strain, we found that sub-inhibitory concentrations of bronopol (2 μg/L and 20 μg/L) significantly increased the conjugative transfer frequency (CTF) of both plasmids. Mechanistic analysis revealed that bronopol enhanced bacterial membrane permeability, as demonstrated by propidium iodide (PI) staining, 1-N-phenylnaphthylamine (NPN) fluorescent probes, transmission electron microscopy (TEM), and upregulation of the outer membrane protein gene ompC. Additionally, bronopol treatment upregulated RP4 plasmid-encoded genes involved in DNA transfer/replication (trfAp) and the global regulator of HGT (kilA/kilB). These findings highlight a previously unrecognized role of bronopol in facilitating the dissemination of antibiotic resistance genes, particularly those of clinical significance.
研究领域:
微生物学
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环境科学
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遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2023
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IF 4.1
Mengqiang Wang,Li Jia,Xun Jiao,Tangwei Zhang,Ming Xiao,Miao Wang,Shanshan Wang,Lufei Zheng,Jing Wang,Yongxin She,A.M. Abd EI-Aty
Fish farming plays a vital role in providing food, nutrition, and employment globally. However, this industry faces security challenges, necessitating the use of fungicides and preservatives, such as bronopol, to increase product yields. Bronopol (2‑bromo-2-nitropropan-1,3-diol; CAS:52–51–7) is widely used in various fields, including food production, cosmetics, and, more recently, aquaculture. Currently, there is a limited number of techniques available for detecting bronopol in aquaculture products. This is primarily due to bronopol's instability, susceptibility to degradation, and tendency to form precipitates that pose challenges in extraction from aquaculture products. For this issue, this study presents a comprehensive method for detecting bronopol content in aquaculture tissues using liquid chromatography-tandem mass spectrometry (LC‒MS/MS). The methodology was optimized, involving extraction with Cu-Zn precipitant, cleanup using a small HLB column, separation on a T3 column, and gradient elution with water and acetonitrile mobile phases. The quantitative approach was employed without the use of an internal standard, following the external standard method. The spiked recoveries at 3 fortification levels (0.1, 0.2, and 1 mg/kg) ranged from 87.1 % to 108.1 % with relative standard deviations RSD ≤ 9.0 %. By applying this method to fresh fish, shrimp, crab, and shellfish samples from a local supermarket, no residues of bronopol were detected, ensuring the reliability of the results. The simplicity, rapidity, and high sensitivity of the method make it a suitable alternative to conventional techniques for bronopol detection. Moreover, the successful validation of the method's recovery and precision supports its potential application in monitoring and preventing the misuse of bronopol in aquaculture, thereby safeguarding aquaculture product quality and protecting public health.
研究领域:
食品科学
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分析化学
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水产养殖
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PROGRESS IN ORGANIC COATINGS
·
2026
·
IF 7.9
Ruiyang Yang,Dakai Gong,Yanting Han,Yuanzhang Jiang,Lin Tan
Developing durable antibacterial and antifouling fluorine-free waterborne polyurethane (WPU) coatings for diverse substrates remains challenging. In this study, a prepolymer synthesis strategy was developed to prepare WPU (GWSiPU) with tailored contents of polydimethylsiloxane (PDMS) and glycerol monostearate (GMS), while incorporating bronopol as an antibacterial component. The GWSiPUs containing 4 wt% PDMS and 20 wt% GMS exhibited persistent antifouling behavior and superior antibacterial ability, achieving 99.9% antibacterial efficiency against E. coli and S. aureus . When applied to cotton fabrics, the coating rendered the fabric surface highly hydrophobic with a water contact angle (WCA) above 145° while maintaining favorable breathability. Various aqueous liquids cannot completely penetrate the coating within 10 min. After 50 cycles of abrasion and repeated washing, the WCA of GWSiPU coated cotton fabric remained above 140°, endowing the fabric with resistance to bacterial adhesion. In addition, the GWSiPU coating also enabled rapid droplet shedding on wood and prevented corrosion of metal after 30 days of immersion in a 3.5 wt% NaCl solution. This work demonstrates a fluorine-free strategy for constructing multifunctional WPU coatings that integrate antibacterial activity, antifouling performance, and long-term durability across diverse substrates.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）