Tianwen Liu,Wei Yuan,Mengyao Si,Xiaoning Wang,Xiangping Liu,Xiaoying Wang
The concentration fluctuations of cellular-myelocytomatosis oncogene (c-Myc) protein may be related to a variety of tumors such as lymphoma, therefore, detection of abnormal trace-level changes is of great significance for early predictions. Conventional detection methods suffer from time-consuming analytical processes and insufficient sensitivity. Thus, the development of a sensitive detection method for c-Myc protein in blood is particularly crucial. We developed an electrochemical sensor for the sensitive detection of c-Myc protein based on gold–palladium alloy nanoparticles (AuPd NPs), leveraging its dual-function for both electrode modification and signal tag. Specifically, AuPd NPs were rapidly formed on the electrode surface by electrodeposition techniques to anchor the capture antibodies (Ab I ), which can increase the conductivity and load capacity of the electrode. Subsequently, using toluidine blue (TB) as a reducing agent, TB-AuPd was synthesized in one step, and further utilized to anchor the detection antibodies (Ab II ), which improved the load quantity and electrocatalytic activity of TB. When the target was present, the sandwich sensor was successfully constructed, benefiting from dual amplifications to achieve sensitive detection of c-Myc protein. Eventually, the proposed sensor exhibited a wider linear range (1–1000 pg mL −1 ) and a lower detection limit (0.5 pg mL −1 , S/N = 3). The developed method demonstrated strong concordance with commercially available ELISA kits, with no statistically significant difference observed in detection results. Thus, the proposed sensor realized sensitive detection of c-Myc protein, and can render valuable guidance for prospective clinical test applications.
Yijing Kuang,Wei Yuan,Xuyuan Chen,Xiaoning Wang,Xiaoying Wang
Background The expression of oncoprotein c-Myc (OP c-Myc ) in blood is closely related to the occurrence and development of various tumors. Common detection methods have the disadvantages of complicated procedures, expensive instruments, or low sensitivity. Currently, several single-mode immunosensors have been developed to detect OP c-Myc and overcome the above shortcomings. However, they are susceptible to the interference of the sensing interface and the external environment, which may lead to false-positive or false-negative results. Therefore, sensitive and accurate assessment of OP c-Myc levels in the blood is very important for the early diagnosis and monitoring of tumors. Results A novel electrochemiluminescence (ECL)-electrochemical (EC) dual-mode immunosensor was developed for the sensitive detection of trace OP c-Myc . Gold nanoparticles-magnetic reduced graphene oxide (AuMrGO), with a large surface area and conductivity, were stably fixed on a magnetic glassy carbon electrode and utilized as the immobilization platform. Ruthenium(II)tris(bipyridine) (Ru) doped Au, Ag, and Pd trimetallic nanocomposite (Tri-Ru) with excellent ECL and EC properties was synthesized by mechanical alloying method and used as the bifunctional signaling label. Further, combined with the trace detection advantages of indirect competition, the convenient and sensitive dual-mode detection of OP c-Myc was realized. The results showed a strong linear relationship for concentrations from 10 pg L −1 to 0.1 μg L −1 in ECL mode and from 0.1 ng L −1 to 1 μg L −1 in EC mode, with limits of detection (LOD) of 4.9 pg L −1 and 30 pg L −1 , respectively. The assay performed good analytical performances and clinical practicability in lymphoma patients’ blood samples. Significance This is the first detection of OP c-Myc using an ECL immunosensor, and it is also the first attempt to use a dual-mode detection strategy for OP c-Myc . The strategy could be used for sensing and detecting other proteomic tumor markers and established a promising platform for early auxiliary diagnosis and monitoring of cancers.
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