Analytical Methods
·
2024
·
IF 2.7
Yang Zhen,Jingyuan Chen,mengyu Liu,Jiabao Huang,Jieping Liang,Mengjiao Zhu,Yuanwei Shen,Danqing Li,Chong Lei,Xuecheng Sun
An integrated magnetoimpedance (MI) biosensor microfluidic magnetic platform was proposed for the evaluation of of cardiac markers cardiac troponin-I (cTnI). The bioanalytes evaluation platform mainly contained external permanent magnets (PMs), MI elements, peelable SiO2film units and microfluidic chip (MFC). The MI elements were made of micro-electro-mechanical system (MEMS)-based multilayered [Ti(6 nm)/FeNi(100 nm)]5/Cu(400 nm)/[Ti(6 nm)/FeNi(100 nm)]5 thin films and designed as meander structures with closed magnetic flux. The MFC was fabricated by using 3D printing and inverted molding techniques, and designed with a solution mixing reaction region, a magnetic separation region and detection regions. Peelable SiO2 film units with the same size as the MI sensing element are used for bioanalytes immunoreactivity interface. Two large PMs were placed directly below the MI sensing unit to provide a bias magnetic field, and the smaller PM was embedded in MCF for magnetic separation function. The different concentration biological target (cTnI antigen), PBS buffer and Dynabeads labeled polyclonal cTnI antibody solution are injected sequentially into the MCF. After immunoreactivity and magnetic separation, a classical sandwich immunoreaction process occured on the surface of the monoclonal antibody-modified SiO2 film via self-assembling technology in the reaction region of MFC. The fundamental principle for evaluation of cTnI was based on variation of the MI signal under different concentration biological target coupled with different number of Dynabeads. It was demonstrated that the mentioned MI-based magnetic platform can perform detection analyses over a range of cTnI concentrations (lowest concentration 0.1 ng/ml and highest concentration 100 ng/ml). The proposed MI-based magnetic platform provides a sensitive, reliable, stable and reusable bioanalytical platform and has potential for future biomedical applications.
研究领域:
生物医学工程
·
诊断学
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PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Linling Zhong,Fanpeng Gong,Jing Li,Guizhu Liu,Xin Wen,Fan Yu,Changjun Hou,Sen Yang,Fangyong Zhu,Xianfeng Wang
High-performance detection of cTnI possesses great significance for diagnosis of myocardial infarction. In this work, two kinds of lower-dimensional Cu(II)-loaded polydopamines were synthesized via in-situ polymerization of dopamine for the first time using Cu-pBDC MOFs and Cu-aBDC MOFs as templates. The obtained Cu-ppDA nanosheets and Cu-apDA nanoribbons maintain the original structure of MOFs and exhibit the advantages of large specific surface area and metal coordination effect, which efficiently enhances the adsorption of single-stranded aptamer DNA probe via π–π stacking. Energy transfer from FAM-Apt probe to Cu(II)-loaded pDA occurred, resulting in the fluoresce quenching. The inference of target cTnI assembles with FAM-Apt probe and the releases from FAM-Apt/Cu-ppDA or FAM-Apt/Cu-apDA systems, leading to the fluorescence recovery. This approach enables a simple, rapid, sensitive, and specific detection method for cTnI. By contrast, FAM-Apt/Cu-ppDA system shows a lower LOD than that of FAM-Apt/Cu-apDA. Furthermore, the successful implementation of these two fluorescent sensors for the detection of cTnI in serum samples obtained from mice with myocardial infarction has been demonstrated. The outcomes of this detection aligned with those achieved through the ELISA method, suggesting their promising potential for practical application in early screening for myocardial infarction.
研究领域:
生物医学工程
·
分析化学
·
医学诊断
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DOI
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-11
·
IF 14.7
Li Juanhua,Liu Yiren,Wu Tianyu,Xiao Zihan,Du Jianhang,Liang Hongrui,Zhou Cuiping,Zhou Jianhua
Acute myocardial infarction (AMI) has become a public health disease threatening public life safety due to its high mortality. The lateral-flow assay (LFA) of a typical cardiac biomarker, troponin I (cTnI), is essential for the timely warnings of AMI. However, it is a challenge to achieve an ultra-fast and highly-sensitive assay for cTnI (hs-cTnI) using current LFA, due to the limited performance of chromatographic membranes. Here, we propose a barbed arrow-like structure membrane (BAS Mem), which enables the unidirectional, fast flow and low-residual of liquid. The liquid is rectified through the forces generated by the sidewalls of the barbed arrow-like grooves. The rectification coefficient of liquid flow on BAS Mem is 14.5 (highest to date). Using BAS Mem to replace the conventional chromatographic membrane, we prepare batches of lateral-flow strips and achieve LFA of cTnI within 240 s, with a limit of detection of 1.97 ng mL −1 . The lateral-flow strips exhibit a specificity of 100%, a sensitivity of 93.3% in detecting 25 samples of suspected AMI patients. The lateral-flow strips show great performance in providing reliable results for clinical diagnosis, with the potential to provide early warnings for AMI.
研究领域:
生物医学工程
·
心脏病学
·
分析化学
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PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Hong Yang,Shanshan Zhu,Lihua Pang,Yifei Ma,Xiaoxue Liu,Jinhua Liu
ABSTRACT Using a single emission-wavelength to accurately/sensitively quantify disease markers in complex environments still faces enormous challenges. In this study, we construct a Cu 2+ -triggered wavelength-tunable fluorescent immunoassay for real-time detection of cTnI in human serum. The developed platform is based on an “on-off-on” strategy, which involves the rapid in-situ fluorogenic reaction of dopamine (DA) and phenol analogue by Cu 2+ , strong coupling between PPi and Cu 2+ as well as the specific hydrolysis of PPi into orthophosphate by alkaline phosphatase. By using cTnI as a model antigen, the assay shows good linearity relationships between the fluorescence response and cTnI concentration. Furthermore, we used different phenol analogues (1,3-dihydroxynaphthalene, 8-hydroxyjulolidine, 1,5-dihydroxynaphthalene) as fluorescence reaction substrates to achieve multi-channel (blue, green and yellow) real-time monitoring of cTnI with detection limits of 0.17 ng/mL, 0.17 ng/mL and 0.33 ng/mL, respectively. We successfully applied the wavelength-tunable ELISA to detect cTnI levels in real human serum, and the results were compared with a commercial ELISA with satisfactory outcomes. Therefore, our proposed platform demonstrates great potential in the early diagnosis of acute myocardial infarction.
研究领域:
分析化学
·
生物化学
·
医学诊断
·
心脏病学
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DOI
高影响因子
Analytical Methods
·
2024
·
IF 2.7
Luhai Wang,Jiaze Sun,Siwei Dai,Pengfei Zhang,Yefei Zhu,Yu Zhang
Myocardial fibrosis is a pathological condition characterized by the excessive accumulation of extracellular matrix proteins, primarily collagen, within the myocardium. Early symptoms of myocardial fibrosis are often subtle, leading to late detection. As the disease progresses, it is often associated with life-threatening complications. Therefore, myocardial fibrosis urgently demands a diagnostic system that is rapid, effective, and preferably non-invasive. In this study, we developed a highly sensitive microfluidic light-initiated chemiluminescent assay (LICA) for the simultaneous detection of cTnI, NT-proBNP, HA, LN, PICP, and PIIINP, the key biomarkers involved in the occurrence and development of myocardial fibrosis. The system is highly integrated and compact, incorporating reagent lyophilization and whole blood separation technologies, and is suitable for point-of-care testing. By combining advantages of centrifugal microfluidics and LICA, the system effectively shortens the detection time for key biomarkers associated with MACEs. Moreover, the system provides optimal analytical performance, with LoDs of 0.0018 ng mL−1, 0.0127 ng mL−1, 3.52 ng mL−1, 6.88 ng mL−1, 4.68 ng mL−1, and 0.072 ng mL−1 for cTnI, NT-proBNP, HA, LN, PIIINP and PICP, respectively. Ultimately, the system can achieve an AUC of 0.548, 0.670, 0.772, 0.752, 0.833 and 0.713 for cTnI, NT-proBNP, HA, LN, PIIINP, and PICP, respectively, and an overall combined ROC of 0.946 for the detection of myocardial fibrosis, indicating its excellent efficacy and potential for widespread clinical applications.
研究领域:
医学诊断
·
生物工程
·
心血管病学
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DOI
PubMed
高影响因子
Analytical Methods
·
2026
·
IF 3
Liansheng Ling,chen chen,Yingqing Li,xiaosong Yang,Ji Zhang
Here we report an ultrasensitive dynamic light scattering (DLS) immunosensor for cardiac troponin I (cTnI) leveraging a novel Self-cascading reaction synergistic amplification strategy by using metal organic frameworks (MOF) materials. This synergistic amplification system was designed within combined enzymatic (glucose oxidase, GOD) catalysis, acid-responsive MOF disintegration, Fenton reaction and gold nanoparticles (Au NPs) stability modulation. When the target cTnI was present, sandwich structure was formed between multifunctional probes (Ab-GOD/Fe-MOF) and Ab modified 96-well plates, subsequently occurring self-cascading reactions with further addition of glucose: the glucose was oxidized into gluconic acid (creating an acidic micro-environment) by O2 under the catalysis of MOF - conjugated GOD and produced H2O2, the acidic micro-environment triggered the breakdown of Fe-MOF and the release of Fe2+, reacting with H2O2 via the Fenton reaction to etch the protective DNA layer that modified on Au NPs, enable the salt-induced aggregation of AuNPs. Crucially, the DLS assay achieved a low limit of detection (LOD) of 1.5 × 10-13 g/mL, furthermore, this cascade amplification strategy realized the quantitative detection of cTnI in clinical samples with high sensitivity and specificity.
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高影响因子
iScience
·
2025
·Vol. 29
·期 1
·
IF 4.1
Yuqi An,Junling Chen,Tao Ma,Dandan Xu,Feng Liang
Conventional lateral flow immunoassays (LFIAs) are unable to achieve the rapid diagnosis of Acute Myocardial Infarction (AMI) via cardiac troponin I (cTnI) detection due to their limited sensitivity and speed. To overcome this, a nanozymatic magnetic nanomotor (Fe 3 O 4 @PDA@AuNS, MPAuNS) is proposed as an active nanoprobe. The nanoprobe, composed of a Fe3O4 core and a PDA shell where gold nanostars grow, enables multimode detection, including colorimetric, photothermal, and catalytic LFIAs, significantly boosting signal intensity. Specifically, the magnetic property of Fe 3 O 4 and the glucose oxidase-like catalytic activity of AuNS endow the MPAuNS nanoprobes with both magnetotaxis behavior and enhanced diffusion, which respectively enhance the flow rate and reduce probe residue in the test strip, thus improving detection speed and sensitivity within a given assay time. Such a platform offers diverse detection options for the rapid and highly sensitive diagnosis of cTnI, holding great potential for medical guidance on the early diagnosis of AMI.
研究领域:
纳米生物技术
·
即时检测
·
免疫分析
·
心脏生物标志物
·
分子诊断
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DOI
PubMed
高影响因子
TALANTA
·
2026
·
IF 6.7
Jingjing Yin,Yuehua Wang,Shanshan Zhu,Xiaoxue Liu,Yujie Sun,Hong Yang,Yifei Ma,Jinhua Liu
The development of wavelength-tunable fluorescence immunoassays for sensitive biomarker detection remains a significant challenge in clinical diagnostics. In this work, we present an HRP-induced in situ fluorogenic immunoassay platform based on the rapid reaction between p-phenylenediamine (PPD) analogues and 6-hydroxyquinoline (6-Hy). The proposed mechanism involves HRP-catalyzed generation of hydroxyl radicals from H 2 O 2 , which subsequently oxidize PPD to form fluorescent copolymer nanoparticles (FCNPs) with 6-Hy. By strategically modifying the PPD structure with various substituents, we achieved discrete and tunable emission of FCNPs, with maxima spanning from 380 to 600 nm (Δλ ≈ 220 nm). Using cardiac troponin I (cTnI) as a model antigen and zinc-coordination nanoparticles (ZnNPs) as carriers, the immunoassay demonstrated exceptional performance, with a wide dynamic range (0.5-125 ng/mL) and an ultralow detection limit (0.17 ng/mL) for cTnI. The platform was successfully validated in human serum samples, showing excellent correlation with clinical standards. This work not only establishes a novel strategy for wavelength-programmable immunoassays but also provides a versatile platform for advancing biomarker detection in clinical diagnostics.
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高影响因子
ACS Applied Materials & Interfaces
·
2026
·Vol. 18
·期 30
·40189-40199
·
IF 7.8
Ren Zixuan,Xu Weimin,Zhao Jiajia,Cui Yue
Lateral flow immunoassays (LFAs) are widely used because
of their
simplicity, rapid readout, and low cost; however, quantitative performance
is often limited by insufficient signal intensity and background interference
from the membrane substrate. This issue becomes more severe when high-brightness
fluorescent nanolabels are introduced: conventional nitrocellulose
(NC) membranes tend to exhibit autofluorescence and strong light scattering
and can also promote nonspecific retention of nanoparticles, which
elevates background and compromises quantitative reliability. Here,
we present a “fluorescent nanodiamond–electrospun fibrous
membrane” strategy, in which a fibrous electrospun cellulose
acetate (ECA) membrane replaces the NC membrane and photostable nitrogen–vacancy
center nanodiamonds (NVDs) serve as fluorescent labels. The uniform
fibrous architecture of the ECA membrane reduces scattering and substrate-derived
fluorescence; meanwhile, the fibrous network facilitates smoother
convective wash-through, mitigating nonspecific adsorption and mechanical
trapping of nanoparticles. As a result, unbound labels are more efficiently
removed, leading to suppressed background signals and improved quantitative
robustness. Using this platform, we achieved sensitive detection of
cardiac troponin I (cTnI) with a limit of detection of ∼0.009
ng/mL, markedly better than conventional NC-based LFA strips. Clinical
testing further confirms the diagnostic performance and consistency
of the proposed approach. This work offers a simple route to enhance
LFA sensitivity and quantification and is readily extendable to other
point-of-care biosensing applications.
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高影响因子
ELECTROANALYSIS
·
2016
·Vol. 28
·期 9
·2044-2049
·
IF 2.471
Juanjuan Zuo,Xiaoyu Zhao,Xiaocui Ju,Shue Qiu,Wenshuai Hu,Ting Fan,Juankun Zhang
A novel molecularly imprinted polymer (MIP) sensor for rapid determination of cardiac troponin (cTnI) was established. Since it can bind to the template molecule cTnI specifically, it can be used to detect concentration of cTnI in serum without much sample pretreatment. What's more, the electrochemical signals depend on the concentration of template molecules. The synthetic sensor possesses advantages including simplicity, high specificity, low cost of preparation, good chemical and mechanical properties, sensitive and label-free determination. The synthetic sensor shows good dynamic linearity at concentration range from 0.05 to 5.00 nM. The limit of detection (LOD) was found to be 0.027 nM. The detection time of whole process was within 5 minutes.
研究领域:
生物医学工程
·
分析化学
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产品关联
DOI
高影响因子
Theranostics
·
2018
·
IF 8.537
Lixin Hong,Kan Wang,Wenqiang Yan,Hao Xu,Qinghui Chen,Yuhui Zhang,Daxiang Cui,Qinghui Jin,Jinghua He
The detection of cardiac markers is critical to the diagnosis of acute myocardial infarction, and immunochromatographic assays are a common tool for point-of-care analysis. Methods : We report a multiplexed lateral flow test strip for simultaneous quantitative detection of cardiac troponin I (cTnI), creatine kinase isoenzyme MB (CKMB), and myoglobin (Myo). Hydrophilic, monodisperse, stable, and carboxyl-modified (COOH-) magnetic nanobeads (MNBs) were used to construct immunomagnetic probes specific to the three cardiac markers. The detection area of the sandwich-style complexes contained three test lines for cTnI, CKMB, and Myo. The magnetic signal intensity of the detection area in the nitrocellulose membrane was measured via a magnetic immunochromatography reader developed in house. Results : To optimize the assay, a modified working buffer was also investigated to improve the detection sensitivity, decrease the background noise, and shorten the detection time. The MNB-based immunochromatography test (MICT) strip offers a wide linear dynamic detection range, rapid detection, high sensitivity, and specificity. The limit of detection was 0.0089 ng/mL for cTnI, 0.063 ng/mL for CKMB, and 0.05 ng/mL for Myo with minimal cross-reactivity. There were 110 clinical human serum samples that were used to evaluate this platform with high correlation. Conclusion : MICT shows great potential as a supplemental method for in vitro diagnostics in the laboratory or in other point-of-care testing (POCT) applications.
研究领域:
医学诊断
·
纳米技术
·
生物医学工程
·
临床化学
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DOI
PubMed
高影响因子
ANALYST
·
2024
·Vol. 149
·期 15
·3961-3970
·
IF 3.6
Yao Xiao,TaoMei Peng,YuXiao Luo,Lei Jiao,TaiXing Huang,He Li
Manganese dioxide (MnO2) nanosheets possess unique physical and chemical properties, making them widely applicable in various fields, such as chemistry and biomedicine. Although MnO2 nanosheets are produced using bottom-up wet chemistry synthesis methods, their scale is below the gram level and requires a long processing time, restricting their effective scale-up from laboratory to market. We report a facile, green and scalable synthesis of MnO2 nanosheets by mixing Shiranui mandarin orange juice and KMnO4 for 30 minutes. We produced more than one gram (1.095) of MnO2 nanosheets with a 0.65 nm mean thickness and a 50 nm mean lateral size. Furthermore, we established a visual colorimetric biosensing strategy based on MnO2 nanosheets for the assay of glutathione (GSH) and cardiac troponin I (cTnI), offering high sensitivity and feasibility in clinical samples. For GSH, the limit of detection was 0.08 nM, and for cTnI, it was 0.70 pg mL−1. Meanwhile, the strategy can be used for real-time analysis by applying a smartphone-enabled biosensing strategy, which can provide point-of-care testing in remote areas.
研究领域:
材料科学
·
生物医学工程
·
分析化学
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DOI
PubMed
高影响因子
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