JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
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2012
·Vol. 26
·期 12
·487-492
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IF 1.38
Jiongwei Pan,Zaiting Ye,Xiaoping Cai,Liangxing Wang,Zhuo Cao
The interaction of ceftriaxone sodium (CS), a cephalosporin antibiotic, with the major transport protein, bovine serum albumin (BSA), was investigated using different spectroscopic techniques such as fluorescence, circular dichroism (CD), and UV–vis spectroscopy. Values of binding parameters for BSA–CS interaction in terms of binding constant and number of binding sides were found to be 9.00 × 10 3 , 3.24 × 10 3 , and 2.30 × 10 3 M −1 at 281, 301, and 321 K, respectively. Thermodynamic analysis of the binding data obtained at different temperatures showed that the binding process was spontaneous and was primarily mediated by van der Waals force or hydrogen bonding. CS binding to BSA caused secondary structural alterations in the protein as revealed by CD results. The distance between CS and Trp of BSA was determined as 3.23 nm according to the Förster resonance energy transfer theory. © 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:487-492, 2012; View this article online at wileyonlinelibrary.com . DOI 10.1002/jbt.21446
研究领域:
生物化学
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分子毒理学
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药物相互作用
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CANADIAN JOURNAL OF CHEMICAL ENGINEERING
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2018
·Vol. 97
·期 3
·709-716
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IF 1.265
Wei Wang,Dapeng Hu,Yanqiu Pan,Hengle Li,Guohua Chen
Initially unsaturated material frozen with prefabricated porosity was prepared to experimentally investigate its effect on freeze-drying of liquid material. Ceftriaxone sodium, a kind of commonly used antibiotic, was selected as the primary solute in an aqueous solution. The liquid nitrogen ice cream-making method was employed to prepare frozen materials with different initial porosities. Results showed that freeze-drying was significantly enhanced with the initially unsaturated frozen material and substantial drying time was saved compared with conventional freeze-drying. Drying time for the unsaturated sample (0.3 of saturation or 0.67 of initial porosity) was more than 20 % shorter than that required for the saturated one (1.0 of saturation without initial porosity). SEM images displayed that the dried products of the unsaturated frozen material had a pierced solid skeleton and uniform pore space with a fine and tenuous structure compared with those of the saturated one. The freezing rate exhibited little influence on the freeze-drying process. Annealing can be moderately beneficial to shortening the drying time and further improving the energy utilization. An appropriate increase in operating temperature was able to enhance the freeze-drying rate while changes in chamber pressure had a negligible effect on the process.
研究领域:
制药工程
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生物医学工程
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Journal of Cleaner Production
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2023
·
IF 11.1
Haoran Chen,Zongping Wang,Yuxin Huang,Junchi Wei,Gang Guo,Lei Miao
Ceftriaxone sodium (CTX) is a typical β-lactam antibiotic in pharmaceutical wastewater. Although traditional biological treatments are low-cost and eco-friendly, antibiotics usually inhibit microorganisms , resulting in limited treatment performance. The biological co-metabolism process provides carbon sources and energy for microorganisms through co-substrate addition, thereby producing enzymes or cofactors to enhance the degradation of refractory pollutants. Therefore, the present study aimed to investigate the degradation performance of CTX through anaerobic biological co-metabolism. A simple co-metabolic substrate (i.e., glucose, 80 mg L −1 ) was added into an anaerobic-activated sludge system, and its mechanism was explored in terms of the variations in extracellular polymeric substances , microbial structure, and resistance genes. Additionally, the possible degradation pathways of CTX were described. The results showed that the degradation efficiency of CTX (200 mg L −1 ) was only about 35% within 8 h in the control group, while it increased to 67.6% in the experimental group with glucose. Chloroflexi , Actinobacteria , Bacteroidetes ADurb.Bin123, and Bacteroidales were the predominant bacterial species in the system. Additionally, the types of enzymes and the relative abundance of β-lactam resistance genes in the co-metabolic system significantly increased by 7 and 0.77%, respectively. Furthermore, nine primary anaerobic CTX biodegradation products were detected, indicating the existence of three possible degradation pathways. Overall, the study results provide a novel approach to treating CTX-containing wastewater.
研究领域:
环境科学
·
微生物学
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Scientific Reports
·
2025
·Vol. 15
·期 1
·1-11
·
IF 3.9
Zhang Liwen,Zhao Jinhong,Zhang Jie,Lou Bang,Li Huijie,Guo Fangyuan,Yang Gensheng,Hong Weiyong
The convergence of advanced materials science and additive manufacturing technologies has revolutionized medical applications ranging from orthodontic devices to patient-specific bone fixation systems. Despite these advancements, the clinical translation of 3D-printed orthopedic implants remains constrained by suboptimal biocompatibility of conventional manufacturing materials. This study systematically evaluates dental resin-based composites as alternative biomaterials for bone fixation devices, hypothesizing that their rapid photopolymerization kinetics, mechanical robustness, and inherent biocompatibility can address current limitations. Through stereolithography technology, ceftriaxone sodium-loaded bone fixation devices were developed with customized geometries tailored to diverse anatomical requirements. Material optimization revealed that a 7:3 ratio of ethoxylated bisphenol A dimethacrylate (BPA2EODMA) to triethylene glycol dimethacrylate (TEGDMA) achieved optimal mechanical performance. Cytocompatibility assessment using L929 fibroblasts demonstrated > 70% cell viability, confirming minimal cytotoxicity. The drug-eluting implants exhibited potent antimicrobial efficacy in 8-h elution assays, showing inhibition rates of 49.14 ± 4.89% ( Staphylococcus aureus ), 48.38 ± 5.88% ( Escherichia coli ), and 26.79 ± 2.69% ( Candida albicans ). These results validate the dual functionality of antibiotic-loaded dental resin constructs, positioning them as promising candidates for patient-specific orthopedic solutions that simultaneously address mechanical stability, osseointegration, and infection prevention.
研究领域:
生物医学工程
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材料科学
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药理学
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骨科
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CHEMOSPHERE
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2022
·
IF 8.943
Chen Zhang,Yingzhang Shi,Zhiwen Wang,Cheng Liu,Yidong Hou,Jinhong Bi,Ling Wu
ZnIn 2 S 4 ultrathin 2D nanosheets with a positive surface charge are synthesized by a hydrothermal method and different contents of surface S vacancies are induced via heat treatment of as-prepared ZnIn 2 S 4 (ZIS). As the S vacancies contents increased, the photocatalytic degradation efficiency of ceftriaxone (CTRX) sodium is promoted. Especially, ZIS-300 shows the best degradation efficiency (88.8%) for an initial CTRX concentration of 10 mg L −1 in 2 h. It is found that S vacancies cause the electron density of surface metal atoms (Zn, In) to be decreased, which makes the effective adsorption and activation of ceftriaxone anions through electrostatic adsorption interactions. Meanwhile, S vacancies also serve as active centers to promote the absorption of O 2 and gather electrons to form •O 2 − species. The photogenerated holes quickly transfer to the surface of the catalyst to directly degrade the adsorbed CTRX. Thus, the photocatalytic CTRX degradation efficiency is significantly improved. Finally, a possible mechanism for over defective ZIS is proposed. This work provides a feasible strategy for the efficient degradation of antibiotics from the perspective of electrostatic adsorption and molecule activation.
研究领域:
环境科学
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材料科学
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化学
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药学
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Journal of Water Process Engineering
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2026
·
IF 6.7
Junchi Wei,Haoran Chen,Daotong Zhao,Jinlong Wang,Miao Zhang,Lei Miao
Bioaugmentation can effectively improve biodegradation of recalcitrant compounds through adding specific substances or microbial consortia. In this study, a small quantity of co-metabolic sludge was introduced to conventional anaerobic sludge system (with a ratio of 1:4 between co-metabolic sludge to conventional anaerobic sludge), and enhancement effect on ceftriaxone sodium (CTX) biodegradation was investigated. The addition of co-metabolic sludge significantly improved CTX removal efficiency in anaerobic sludge system (with an average increase of 20.6 %), and the maximum removal of CTX reached 60.8 %. Co -metabolic sludge also elevated extracellular polymeric substance (EPS) secretion, increasing protein and polysaccharide contents by 34.66 % and 40.64 %, respectively, which mitigated CTX toxicity. Compared to conventional sludge, the system after bioaugmentation exhibited 4.3 % and 2.5 % increases in enzyme diversity and microbial population, respectively. Relative abundances of resistance genes evgS, NmcR, and Burkholderia pseudomallei Omp38 increased by 2.8 %, 7.6 %, and 2.5 %, respectively, which benefited improving CTX degradation. Furthermore, the abundances of pathways Two-component system (TCS) and Quorum sensing (QS) increased by 20.0 % and 21.7 %, respectively, indicating their involvement in bioaugmentation-mediated CTX degradation. Overall, this study strengthens CTX degradation in conventional sludge system with addition of some co-metabolic sludge, which provided a novel strategy for CTX wastewater treatment.
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Water Resources and Industry
·
2023
·
IF 5.1
Yonghao Zhang,Yating Song,Liu Liu,Jie Zhang,Zhaoyi Zhang,Qian Li,Jing Yang,Wen Li,Lulu Huang,Xue Li,Yulei Zhang,Qiangshun Wu,Xuejie Guo
In this study, porous Ti/Magnéli Ti 4 O 7 nanotube arrays (NTA) was fabricated to pretreat antibiotic wastewater. Characterization of the Ti 4 O 7 -NTA illustrated that highly ordered NTA was successfully generated on the porous Ti substrate, which enlarged the specific surface area by 1.67 times. The Ti 4 O 7 -NTA has a high oxygen evolution potential (2.60 V) as well as low interfacial charge transfer resistance (4.479 Ω cm −2 ). Ceftriaxone sodium (CRO) was completely removed from raw antibiotic wastewater after 3 h of treatment by the Ti 4 O 7 -NTA, under optimal operation conditions. The biodegradability and toxicity of the wastewater were also significantly enhanced and reduced, as the BOD/COD and EC 50,48h values were increased to 0.48 and 57.62 % ± 2.16 %, respectively. The possible degradation pathway of CRO was proposed by LC‒MS. Cathodic polarization was proven to be an effective way to ensure the long-term durability of the Ti 4 O 7 -NTA.
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DIAMOND AND RELATED MATERIALS
·
2026
·
IF 5.2
Huihu Wang,Shenghao Jiang,Liu Ouyang,Yu Xiong,Qianyu Wang,Yun Zhao,Xing Xiang,Chenhuinan Wei
g-C 3 N 5 /BiVO 4 - x heterojunctions with tunable BiVO 4 facet ratios were fabricated. • g-C 3 N 5 /BiVO 4 -F shows best activity via suitable (121)/(040) and (040)/(011) ratios. • H 2 O 2 addition boosts photocatalytic degradation efficiency of ceftriaxone sodium. • In-situ XPS confirms the S-scheme charge transfer in g-C 3 N 5 /BiVO 4 -F heterojunction. S-scheme heterojunctions have garnered significant attention in the photocatalytic degradation of antibiotics due to the efficient charge separation and strong redox capability preservation. However, photocatalytic activity is highly dependent on the exposed crystal facet ratios of the catalyst. A series of g-C 3 N 5 /BiVO 4 (N-CN/BiVO 4 -x) S-scheme heterojunctions were fabricated by tuning the morphology of BiVO 4 into peanut-like (BiVO 4 -P), flake-like (BiVO 4 -F), and decahedral structures (BiVO 4 -B), thereby controlling the ratios of exposed crystal facets. Degradation performance tests revealed the optimal performance of N-CN/BiVO 4 -F. This heterojunction achieved a degradation rate of 71.1% for ceftriaxone sodium (CS). With the introduction of H 2 O 2 , the degradation rate further increased to 85.1%. The results confirmed that the superior performance of N-CN/BiVO 4 -F is closely related to the specific exposure ratios of its (121)/(040) and (040)/(011) crystal facets. Furthermore, in-situ XPS confirmed that the charge transfer follows S-scheme charge transfer mechanism. Photoelectrochemical measurements and radical trapping experiments revealed a possible CS degradation mechanism. This work elucidates how the exposed facets of BiVO 4 regulate the photocatalytic performance of S-scheme heterojunctions. The findings provide a theoretical and practical basis for designing efficient photocatalysis systems. Download: Download high-res image (92KB) Download: Download full-size image
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DIAMOND AND RELATED MATERIALS
·
2024
·
IF 4.3
Jingle Zhang,Huihu Wang,Yangliu Ou,Hao Tu,Ziguan Zhang,Chenhuinan Wei,Xing Xiang,Ming Bao
As the overuse of antibiotics increasingly contaminates the environment, one of modern chemistry's objectives is to identify high-performance photocatalytic materials to degrade antibiotics. This study initially utilized NH 4 Cl as a gas template to synthesize porous lamellar g-C 3 N 4 (CNS) through a high-temperature calcination process. Building upon this, a homojunction catalyst, CNS/g-C 3 N 5 (S-CN), was developed via a secondary sintering technique using different amount of 3-amino-1,2,4-triazole addition to evaluate its efficacy in activating peroxymonosulfate (PMS) for degrading the antibiotic ceftriaxone sodium. Under neutral conditions, the photocatalytic system composed of S-CN catalysts prepared by 50 mg of 3-amino-1,2,4-triazole addition and PMS (S 3 -CN/PMS) exhibited degradation rates that were 3.37 and 8.11 times higher than those of the CNS/PMS and g-C 3 N 5 /PMS, respectively. At a pH of 8, the S 3 -CN/PMS system attained a 99.7 % degradation efficiency within 75 min. Based on results from UV–visible diffuse reflectance spectroscopy (DRS), transient photocurrent response, electrochemical impedance spectroscopy (EIS), photoluminescence spectroscopy and radical scavenging experiments, this high efficiency is attributed to a homojunction Z-type charge transfer mechanism that enhances the separation of photogenerated carriers. Furthermore, the study found that the optimal PMS addition was 20 mg and the optimal pH was 8. Chloride ions promoted the reaction, while other ions decreased the reaction rate. In conclusion, this paper provides valuable insights for the morphological modification of carbon nitride homojunction materials and the efficient activation of PMS for pollutant degradation.
研究领域:
环境科学
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材料科学
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化学工程
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CHEMOSPHERE
·
2024
·
IF 8.1
Xinyue Feng,Haolin Shi,Wei Liu,Fangwei Ma,Pan Liu,Jiafeng Wan
This study demonstrated the design and fabrication of flower-like Ni/Mn-MOFs materials, and three-dimensional ultrathin flower-like Ni/Mn/MC microspheres were fabricated by embedding metal or metal oxide nanoparticles into a porous carbon skeleton via high-temperature pyrolysis at 600 °C and used for the electrocatalytic degradation of ceftriaxone sodium. This unique ultrathin porous flower-like structure can expose more active sites, provide rapid ion/electron transfer, and improve electrocatalytic activity. Meanwhile, the excellent electrical conductivity of the carbon skeleton, as well as the rational composition and synergistic effect of the two components, can promote the generation of active radicals (•OH and •O 2 − ) in the reaction system, which accelerates the electrochemical degradation process and improves the electrocatalytic degradation performance. The results showed that the Ni/Mn/MC-5:1 composite prepared when the molar ratio of Ni: Mn was 5:1 exhibited the best electrocatalytic degradation performance for the degradation of sodium ceftriaxone. The composites showed 98.2% degradation of ceftriaxone sodium in 120 min and maintained sound degradation after 20 cycles. Therefore, we concluded that this novel multicomponent composite has good electrocatalytic activity and stability for the degradation of antibiotic wastewater.
研究领域:
环境科学
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材料科学
·
化学工程
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2025
·
IF 9.7
Zaimei Li,Hongmei Zhang,Qiangyi Deng,Che Han,Conglu Zhang
In this study, the zirconium-based metal organic framework (Zr-MOF) was applied as the adsorbent for phosphorus (P) pollution in water. Then the phosphate-adsorbed metal organic frameworks (MOFs) were used as a recycled raw material and calcined to obtain P-doped MOFs-derived carbon material (ZrP@Zr-BTC). Next, the ZrP@Zr-BTC was used for peroxymonosulfate (PMS) activation for the ceftriaxone sodium degradation. The doping of P species in the MOFs-derived carbon material led to a 31 % increase in the degradation rate compared to the material without P doping (ZrO 2 @Zr-BTC). The characterization results confirmed that ZrP@Zr-BTC contained zirconium phosphate, ZrP and ZrO 2 in addition to inorganic carbon. P doping could affect the morphology of zirconium species and the bonding state of oxygen element in the catalyst. The degradation of ceftriaxone sodium by the ZrP@Zr-BTC/PMS system could reach 96 ± 0.82 %. The ZrP@Zr-BTC material also had strong resistance to water quality interference and reusability. The electron spin resonance spectrometer (ESR) analysis indicated singlet oxygen ( 1 O 2 ) played an important role and other free radicals (SO 4 − •, •OH, O 2 − •) were auxiliary. The Fukui function calculated by density functional theory explained the sites susceptible to attack by reactive species, and liquid chromatography-mass spectrometry (LC-MS) results allowed for the inference of the degradation pathway of ceftriaxone sodium. This study not only provides a simple and effective method for the disposal and recycling of waste adsorbents but also offers valuable insights into the role of MOF-derived carbon in activating PMS for pollutant degradation.
研究领域:
环境科学
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材料科学
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催化化学
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JOURNAL OF HAZARDOUS MATERIALS
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2022
·
IF 14.224
Jie Chen,Jiafeng Wan,Chi Li,Yuhan Wei,Haolin Shi
Fe 0 -Fe 3 O 4 nanoparticles and cerium dioxide hollow spheres as efficient heterogeneous electro-Fenton reagents were rationally designed to be embedded in porous carbon derived from skimmed cotton for the electrocatalytic degradation of ceftriaxone sodium. Skimmed cotton porous carbon material has a hollow tubular structure, and cerium dioxide is dispersed on the surface of the carbon material in a hollow sphere structure of uniform size. Fe 0 -Fe 3 O 4 nanoparticles were wrapped in irregular particle shapes on the surface of cerium dioxide hollow spheres, and the remaining part was laid flat on the surface of porous carbon material. The as-synthesized Fe 0 -Fe 3 O 4 /CeO 2 /C showed excellent degradation efficiency of 95.59 % for ceftriaxone sodium within 120 mins and obtained a COD removal rate of 95.21 % at 240 mins. The zero-valent iron as a reducing agent effectively accelerated the Fe 3+ /Fe 2+ cycle, allowing the composites to exhibit higher catalytic activity and further reducing the possibility of secondary contamination. Moreover, the existence of cerium dioxide further promoted the redox cycle of Ce 4+ /Ce 3+ and accelerated the electron transfer in the interface of the catalyst. The synergistic effect of iron and cerium greatly facilitated the production of hydroxyl radicals and increased the yield of hydroxyl radicals in the reaction system.
研究领域:
环境科学
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材料科学
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化学工程
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