PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2021
·
IF 3.963
Zhi-Xiang Liu,Xiao-Rong Xing,Xin-Hao Liang,Jian-hao Ding,Yi-Jiangcheng Li,Ying Shao,Fu-An Wu,Jun Wang,Sheng Sheng
Glyphodes pyloalis Walker is a destructive pest on mulberry trees and poses a significant threat to the sericultural industry in China. Phoxim and chlorfenapyr are two commonly used insecticides in mulberry fields. Glutathione-S-transferases (GSTs) comprise a multifunctional protein superfamily that plays important roles in the detoxification of insecticides and xenobiotic compounds in insects. However, whether GSTs participate in the tolerance of phoxim and chlorfenapyr in G. pyloalis is still unknown . To better understand the mechanism of insecticide tolerance in G. pyloalis , the enzymatic activity of GSTs was evaluated under phoxim and chlorfenapyr exposure, respectively. GST enzyme activity was significantly increased after 12, 36 and 48 h of phoxim treatment and 12, 24, 36 and 48 h of chlorfenapyr treatment. Subsequently, eighteen GST genes were identified from the larvae transcriptome of G. pyloalis . Among these, ten GpGSTs had GSH-binding sites and fifteen GpGSTs had variable hydrophobic substrate-binding sites. The expression levels of Delta-GpGST and Epsilon-GpGST genes were significantly influenced by phoxim and chlorfenapyr treatment, and by the time post insecticide application . Furthermore, after silencing GpGST-E4 , the mortality rate of G. pyloalis larvae was increased when they were exposed to chlorfenapyr, but it did not significantly alter when the larvae were exposed to phoxim. Our results indicated the vital roles of GpGSTs in the tolerance of insecticides and this action depends on the categories of insecticides. The present study provides a theoretical basis for elucidating insecticide susceptibility and promotes functional research on GST genes in G. pyloalis .
研究领域:
昆虫学
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分子生物学
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害虫防治
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DOI
PubMed
高影响因子
npj Science of Food
·
2025
·Vol. 9
·期 1
·1-8
·
IF 7.8
Pan Hui,Zhang Wei,Jin Meng,Zhang Fang,Chen Xuelei,Meng Xiao,Shao Hua,Song Liqun,Zhang Zhihu,Wang Cuijuan
Concerns over health risks associated with mixed pesticide residues have led to a demand for rapid analytical methods. We present a portable surface-enhanced Raman spectroscopy (SERS) device specifically designed for real-time monitoring of indoxacarb (INDX) and chlorfenapyr (CFP) in vegetable juice. This point-of-use sensor integrates silver nanoparticles with a metal-organic framework (Ag/MOF-808(Zr)) and employs a 19-well quartz plate alongside a portable Raman spectroscopy instrument, facilitating simultaneous adsorption, separation, and detection. Detection limits for INDX and CFP in ginger and spinach juices were 0.26–0.76 ppb. Notably, results closely matched those from liquid chromatography, and in-situ detection was achieved within 2 min without pre-treatment, significantly enhancing efficiency over conventional chromatographic methods. This innovative approach demonstrates substantial potential for detecting pesticide mixtures in non-laboratory settings, presenting a valuable option for point-of-use applications.
研究领域:
分析化学
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食品安全
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环境监测
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纳米技术
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光谱学
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DOI
PubMed
高影响因子
Toxics
·
2025
·Vol. 13
·期 1
·65
·
IF 4.1
Di Zhang,Xiao-Hua Song,Dan Yang,Mu-Zi Ge,Jun Qiu,Han-Qing Jiang,Yan-Yan Sun,Xiang-dong Li,Yi-Jun Wu
Residues of the pesticides chlorfenapyr (CFP) and emamectin benzoate (EMB) often coexist in the environment and can be accumulated in the body. To understand the impact of these two chemicals on health, we investigated their effect on the kidneys. In this study, rats were treated with CFP and/or EMB at low/medium/high doses of 1/3/9 mg/kg/day and 0.2/0.6/1.8 mg/kg/day, respectively, via oral gavage for 60 days. Kidneys and serum samples were collected and serum biochemistry and kidney histopathological changes were analyzed and examined. Kidney metabolome alterations were analyzed by using gas chromatography–mass spectrometry. The results showed that combined exposure to CFP and EMB elevated BUN levels and induced pathological damage, which presented as thinner renal tubular epithelial cells, an abnormal glomerular morphology, and an increased fibrotic area. CFP and/or EMB disrupted glutathione metabolism and carbohydrate metabolism, resulting in the alteration of kidney metabolomes and inducing oxidative stress in the cells of kidney tissues. In addition, CFP decreased ATP content and inhibited pyruvate PDH activity in the kidneys. These findings suggest that long-term exposure to CFP and EMB at environmentally relevant levels induce alterations in the renal metabolome, oxidative stress, and an insufficient energy supply, which may contribute to renal histopathological damage.
研究领域:
毒理学
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代谢组学
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环境科学
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PubMed
高影响因子
RSC Advances
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2022
·Vol. 12
·期 26
·16918-16926
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IF 4.036
Linfang Zhu,Guangqi Jiang,Jun Cen,Linhuai Li
To improve the utilization rate of chlorfenapyr and make the wall material of chlorfenapyr microcapsules easily degradable, polylactide diol, toluene diisocyanate and 1,4-butanediol were used to prepare a chlorfenapyr microcapsule suspension by interfacial polymerization. The product was characterized by the methods of optical microscopy, scanning electron microscopy and Fourier-transform infrared spectroscopy. The results indicated that the microcapsule particles were spherical, with an encapsulation efficiency of 84.20%. The diluted product had good wetting and spreading abilities on cabbage leaves. Compared with other commercial formulations, the slow-release effect of the microcapsule suspension was more obvious and the release mechanisms conform to Fickian diffusion, with the release rate controllable by adjusting the external pH conditions. Furthermore, the wall material of the microcapsules showed good degradation performance in a phosphate-buffered solution. Microencapsulation by this method significantly increased the validity period of chlorfenapyr and the wall material was also degraded easily.
研究领域:
农药科学
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材料化学
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环境科学
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DOI
PubMed
高影响因子
ARCHIVES OF TOXICOLOGY
·
2026
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IF 10.9
Deng Li,Yan Hanwen,Qian Zhipeng,Zhong Jiaqi,Xie Wenpei,Wang Jianfeng,Xu Jun,Gao Hong,Zong Liang,Chen Xijing,Huang Haoyan,Zhang Yongjie
Chlorfenapyr (CFP) is a halogenated pyrrole insecticide that requires metabolic activation to exert toxicity and has been associated with severe poisoning in humans. However, the metabolic pathways underlying CFP bioactivation and detoxification in humans remain poorly defined. In this study, we systematically investigated the metabolic fate of CFP and its toxic metabolite tralopyril (TLP) using in vitro human drug-metabolizing enzyme systems. Screening of ten human cytochrome P450 (CYP) isoforms at 5 and 50 µM CFP indicated that CYP2B6 is the major contributor to CFP bioactivation, which was further supported by selective inhibition and correlation analyses. CFP metabolism by CYP2B6 followed Michaelis–Menten kinetics, with showing a K m of 1.80 ± 0.57 μM, a V max of 0.24 ± 0.02 pmol/min/pmol P450, and an CL int of 139.78 ± 33.70 nL/min/pmol P450. In addition, incubations using 50 or 100 µM TLP and varying concentrations of glutathione (0.1 to 1 mM GSH) showed the formation of a novel GSH conjugate of TLP (TLP-GSH), of which level was markedly enhanced by human glutathione S-transferases (GSTs). Among six recombinant human GST isoforms tested, GSTA1, GSTA2, GSTM1, and GSTP1 showed significant catalytic activity in TLP-GSH formation compared with non-enzymatic reactions. Moreover, extracellular flux analysis revealed that TLP dose-dependently inhibited basal and maximal oxygen consumption rates, an effect exacerbated by GST inhibition, indicating a protective role of GSTs in TLP-induced mitochondrial toxicity. Together, these findings delineated metabolic enzymes-mediated bioactivation and detoxification pathways governing CFP toxicity in humans and provided a new perspective on clinical management strategies for CFP poisoning.
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PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 58
·121748-121758
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IF 5.8
Wang Xiaoyao,Hu Shuai,Meng Lingtao,Wang Kai,Zhang Xianxia,Li Ke,Wang Ning,Zou Nan,Xu Yue,Li Beixing,Mu Wei,Pang Xiuyu
Emamectin benzoate (EB), chlorantraniliprole (CTP), chlorfenapyr (CFP), and lufenuron (LFR) are widely used to control Spodoptera exigua on cabbage. This study is aimed at establishing a universal, sensitive, accurate, and efficient method for the determination of these pesticide residues in cabbage using QuEChERS pretreatment combined with ultra-performance liquid chromatography or gas chromatography-tandem mass spectrometry (UPLC‒MS/MS or GC‒MS/MS). The recoveries of these pesticides (containing metabolites) in cabbage detected by the optimized method ranged between 80.9% and 99.9%, with relative standard deviations (RSDs) of 0.164–12.5%. The limit of quantification (LOQ) of the four pesticides was determined to be 0.01 mg/kg. The standard curve, accuracy, precision, and LOQ of the analysis method all met the requirements of pesticide residue detection. The optimized method was used to detect the dissipation dynamics and terminal residues in 12 regions. The dissipation half-lives of CTP, CFP, and LFR were 3.35–7.01 d, 2.29–4.75 d, and 3.24–6.80 d, respectively. The terminal residues of all these pesticides were below the maximum residue limits (MRLs). The dietary risk assessment indicated that the dietary risk probabilities for EB, CTP, CFP, and LFR were all less than 1 and were within the acceptable range. This study provides a comprehensive assessment of the residues and dietary risks of EB, CTP, CFP, and LFR for the scientific use of pesticides. Graphical Abstract
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高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2025
·
IF 4
Zhengyi Mao,Wen Zhao,Dongping Chen,Zhiyan Jiang,Qiang Liu,Jiayun Li,Yunxuan Wu,Nannan Lv,Jianting Fan
The pine-forest guardian Dastarcus helophoroides mainly rely on olfaction to locate its host accurately and interact socially. Odorant binding proteins of D. helophoroides play an important role in olfactory recognition and transporting odors to olfactory receptors to trigger signal transduction. However, some compounds affect olfactory recognition of D. helophoroides by binding to odorant binding proteins. In this study, DhelOBP4 and DhelOBP21, which are involved in the recognition of plant volatiles, were expressed to explore compounds that affect olfactory recognition. The affinity of 24 compounds with DhelOBP4 and DhelOBP21 was investigated by vitro fluorescence competitive binding assays. It was found that cyetpyrafen, chlorfenapyr and spirodiclofen had high affinity with DhelOBP4 and DhelOBP21. At the same time, we found that these three pesticides had a good evasive effect on the D. helophoroides and elicited EAG response in a dose-dependent manner. Besides, it was found that silencing DhelOBP4 and DhelOBP21 would affect D. helophoroides identifying these three pesticides. Through homology modeling and molecular docking, we identified key amino acid sites involved in the binding of DhelOBP4 and DhelOBP21 to these three pesticides, which might revealed their specific binding molecular interactions. This study is helpful to provide a valuable addition of OBP-pesticide interactions of D. helophoroides .
研究领域:
昆虫学
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农药科学
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分子生物学
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生物化学
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生态学
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神经生物学
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DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2018
·
IF 3.44
Yunhe Zhao,Qiuhong Wang,Jinfeng Ding,Yao Wang,Zhengqun Zhang,Feng Liu,Wei Mu
Bradysia odoriphaga (Diptera: Sciaridae) is the major pest affecting Chinese chive production. Chlorfenapyr is a halogenated pyrrole-based pro-insecticide that is currently used to control insects and mites on a variety of crops. In the present study, fourth-instar larvae of B. odoriphaga were exposed to chlorfenapyr at LC 1 , LC 20 and LC 50 concentrations. The developmental duration of the treated larvae was not significantly different, but fecundity was significantly increased in the LC 1 and LC 20 treatment groups compared with the control group. The population parameters of the LC 1 treatment group were increased significantly, whereas those of the LC 50 treatment group were reduced significantly compared with the control. The food consumption by larvae and pupal weight were significantly increased under the LC 1 treatment and decreased under the LC 50 treatment compared with the control. Moreover, chlorfenapyr decreased the lipid , carbohydrate and trehalose contents significantly, whereas the total protein content was increased compared with the control. Additionally, the activities of protease, lipase and trehalase were significantly decreased. Chlorfenapyr treatment for 24 h also induced the activities of glutathione S-transferase (GST), carboxylesterase (CarE) and O -demethylation. The results of this study suggest that low lethal concentrations of chlorfenapyr can affect oviposition , population development, the activities of digestion and detoxification enzymes , and nutrient accumulation in B. odoriphaga . This study provides valuable information for the assessment and rational application of chlorfenapyr for effective control of this pest.
研究领域:
昆虫学
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农业科学
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害虫管理
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DOI
PubMed
高影响因子
ANALYST
·
2013
·Vol. 138
·期 22
·6834-6843
·
IF 3.969
Bing Peng,Jiaheng Zhang,Runhua Lu,Sanbing Zhang,Wenfeng Zhou,Haixiang Gao
Two ionic liquid-coated-Fe3O4 magnetic particles (IL-Fe3O4 MPs) were developed for use in two types of dispersive micro-solid phase extraction (D-μ-SPE) for the high-performance liquid chromatographic analysis of clofentezine and chlorfenapyr in environmental water samples. Self-assembling IL-Fe3O4 MPs were used in D-μ-SPE as adsorbents. Two D-μ-SPE extraction methods, namely, direct dispersive micro-solid phase extraction (d-D-μ-SPE) and in situ solvent formation-based dispersive micro-solid phase extraction (ISF-D-μ-SPE), were proposed, using [C8MIM][PF6] to extract analytes through two pathways. Lower IL doses were required in the extraction process compared with those in other IL-based methods. Fe3O4 MPs can also be recycled and reused after extraction and are thus environmentally friendly. These newly developed methods were demonstrated to be feasible for use in the quantitation of clofentezine and chlorfenapyr at trace levels, with lower limit of detection values ranging from 0.4 to 0.5 ng mL−1 for d-D-μ-SPE and 0.4 ng mL−1 for ISF-D-μ-SPE. Finally, relative standard deviations of less than 6.0% were obtained.
研究领域:
分析化学
·
环境科学
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Huiya Chen,Liupeng Yang,Jingtong Zhou,Pengpeng Liu,Shiqi Zhu,Youzhi Li,Suqing Huang,HanHong Xu,Zhixiang Zhang
Spodoptera frugiperda (S. frugiperda) is an invasive pest that threatens global crop production and food security and poses a serious threat to maize production worldwide. Metal–organic framework (MOF) nanocarriers have great potential for agricultural pest control applications. The present study successfully prepared the chemical cross-linking of iron-based metal–organic framework nanoparticles (MIL-101(Fe)-NH2 NPs) with sodium lignosulfonate (SL) as a pH/laccase double stimuli-responsive pesticide release system. The average particle size of the prepared chlorfenapyr (CF)-loaded nanoparticles (CF@MIL-101-SL NPs) was 161.54 nm, and the loading efficiency was 44.52%. Bioactivity assays showed that CF@MIL-101-SL NPs increased the toxicity of CF to S. frugiperda and caused the rupture of the peritrophic membrane and enlargement of the midgut. Data from 16S rRNA gene sequencing showed that CF@MIL-101-SL treatment reduced the resistance of S. frugiperda to pesticides and pathogens and affected nutrient and energy availability by remodeling the intestinal microbiota of S. frugiperda. The dysregulated microbial community interacted with the broken peritrophic membrane, which exacerbated damage to the host. Nontargeted metabolomic results showed that ABC transporters may be a potential mechanism for the enhanced toxicity of CF@MIL-101-SL to S. frugiperda. In summary, the present study provides effective strategies for toxicological studies of nanopesticides against insects.
研究领域:
农业科学
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昆虫学
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纳米技术
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微生物学
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毒理学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Hui Pan,Meng Jin,Fang Zhang,Xuelei Chen,Mengping Zhang,Haiyan Wei,Xiao Meng,Wenwen Chen,Zhihu Zhang,Cuijuan Wang
Health concerns over mixed pesticide residues highlight the need for rapid detection methods. This study developed a multifunctional point-of-use (POU) sensor that integrates separation, enrichment, and surface-enhanced Raman spectroscopy (SERS) using high-index Au trisoctahedrons (TOHs) and metal-organic frameworks (MOFs) on polyamide 6 (PA-6) film. The Au TOHs with sharp tips and high index facets offer excellent SERS performance, while MOFs facilitate analyte enrichment and separation. The flexible PA-6 film was used as an absorbent for the initial adsorption. Using the AuTOHs@ZIF-67/PA-6, we achieved on-site detection of chlorfenapyr (CFP) and emamectin benzoate (EB) in 5 min without pre-treatment, significantly faster than traditional methods. Detection limits were 0.68 ppb for CFP and 0.26 ppb for EB on cucumber surfaces, and 0.13 ppb and 0.95 ppb, respectively, in cucumber juice, well below tolerance levels. This method shows great promise for detecting pesticide mixtures in non-laboratory settings, offering a new POU alternative.
研究领域:
分析化学
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环境科学
·
食品科学
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DOI
PubMed
高影响因子
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