ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2022
·
IF 7.129
Di Ju,Youssef Dewer,Shipan Zhang,Chao Hu,Peirong Li,Xueqing Yang
The codling moth Cydia pomonella L. (Lepidoptera: Tortricidae) is one of the most notorious pests of pome fruits and walnuts worldwide, which has developed resistance to almost all classes of insecticides, including abamectin (ABM). ATP-binding cassette (ABC) transporters are thought to play a vital roles in insecticide detoxification by reducing the toxic concentrations of insecticides in an organism tissues. Despite the tremendous progress in understanding the detoxification mechanisms at the molecular level, the physiological functions of ABC transporters in insects have been poorly investigated. In this study, we found that the ABC inhibitor verapamil synergized significantly the toxicity of ABM, suggesting a potential role of ABC in detoxification. A total of 54 ABC genes were identified in the third-instar larvae of C. pomonella after treatment with sublethal doses (LD 10 and LD 30 ) of ABM. The expression profile of these genes in ABM-treated larvae at different time points (24, 48, 72 hr) using transcriptomic analysis (RNA-seq) was also investigated. The results showed that the expression of about 30 ABC genes was significantly co-upregulated after treatment. Several specific genes were up-regulated at 48 hr after treatment of larvae with LD 10 ABM. Among these up-regulated genes, we found that the relative expression level of the CPOM19553 was 29.7-fold and 16.0-fold higher when larvae were exposed to ABM at the LD 10 and LD 30 doses compared to control, respectively. Unlike other ABC genes, only CPOM08323 exhibited significant expression levels in the head and cuticle of the third-instar larvae of C. pomonella exposed to the two sublethal doses of ABM, with no expression was observed in the detoxification tissues such as midgut and Malpighian tubule. This study suggests that these up-regulated genes may be involved in ABM resistance in C. pomonella . Our findings will provide an additional information required for further analysis of ABC transporter genes associated with xenobiotic metabolism in C. pomonella .
研究领域:
昆虫学
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分子生物学
·
毒理学
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DOI
PubMed
高影响因子
Scientific Reports
·
2016
·Vol. 6
·期 1
·1-10
·
IF 5.228
Liu Wei,Xu Jing,Zhang Runzhi
For successful pest management, codlemone (E, E-8,10-dodecadien-1-ol) is widely used to monitor codling moth. The pheromone release rate is essential for the lure’s attractiveness. The optimal sex pheromone release rate (V 0 ) for trapping codling moth was evaluated during 2013–2014. The overwinter generation V 0 was 6.7–33.4 μg wk −1 , and moth catches (MCs) were 0.82 ± 0.11 adults/trap/week; MCs for lower (V 1 ) and higher (V 2 ) release rates were 52.4% and 46.3%, respectively, of that for V 0 . The first generation V 0 was 18.4–29.6 μg wk −1 , with MCs of 1.45 ± 0.29 adults/week/trap. V 1 and V 2 MCs were 34.5% and 31.7%, respectively, of those for V 0 . Combining across generations, the final V 0 was 18.4–29.6 μg wk −1 , with MCs of 1.07 ± 0.06 adults/week/trap. V 1 and V 2 MCs were 51.4% and 41.1%, respectively, of that for V 0 . Overwinter generation emergence was relatively concentrated, requiring a wider V 0 . Maintaining the release rate at 18.4–29.6 μg wk −1 could optimize the lure’s efficacy; this resulted in the capture of nearly 1.9 and 2.4 times more moths than V 1 and V 2 , respectively. The results also indicate that a dispenser pheromone release rate of 200–300 times that of the female moth can perfectly outcompetes females in the field.
研究领域:
昆虫学
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害虫管理
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农业科学
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DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2019
·Vol. 76
·期 3
·1039-1047
·
IF 3.255
Chao Hu,Wei Wang,Di Ju,Gao-Man Chen,Xiao-Ling Tan,David Mota-Sanchez,Xue-Qing Yang
BACKGROUND Recent work has shown that two codling moth ( Cydia pomonella ) glutathione S -transferase genes (GSTs), CpGSTd1 and CpGSTd3 , can metabolize λ-cyhalothrin, one of the recommended insecticides for C. pomonella control worldwide. However, systematical characterization of delta and epsilon GSTs, especially their potential contributions in the metabolism of λ-cyhalothrin, is currently still lacking in C. pomonella . RESULTS In this study, a total of nine cDNA sequences were identified in C. pomonella , including four in the delta and five in the epsilon subclasses. RT-qPCR showed that seven GSTs were ubiquitously expressed at all developmental stages, and CpGSTe2 , CpGSTe3 , and CpGSTe4 were mainly expressed in larvae. The mRNA levels of CpGSTd2 , CpGSTd4 , and CpGSTe5 were significantly higher in male than in female adults. Tissue-specific expression analysis revealed that the CpGSTe2 , CpGSTe3 , and CpGSTe4 were highly expressed in the midgut while CpGSTd2 and CpGSTd4 were predominantly expressed in the Malpighian tubules. The transcripts of these GSTs (except CpGSTe1 ) were co-expressed following exposure to LD 10 of λ-cyhalothrin for 3 h. Recombinant CpGSTd4, CpGSTe2, and CpGSTe3 proteins expressed in Escherichia coli displayed glutathione-conjugating activity toward 1-chloro-2,4-dinitrobenzene. In addition, λ-cyhalothrin could inhibit the activity of recombinant CpGSTd4, CpGSTe2, and CpGSTe3 enzymes, but only recombinant CpGSTe3 showed λ-cyhalothrin metabolic capacity, with 21.88 ± 1.09% of parental compound being depleted within 1 h. CONCLUSION These data show that CpGSTe3 is a third GST gene, encoding an enzyme that metabolizes λ-cyhalothrin in C. pomonella . © 2019 Society of Chemical Industry
研究领域:
昆虫学
·
分子生物学
·
农药科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Bing Bai,Shi-Pan Zhang,Yu-Ting Li,Ping Gao,Xue-Qing Yang
The advantageous characteristics of invasive pests, particularly their ability to reproduce and adapt to the environment, have been observed. However, it remains unclear what specific inherent superiority enables fruit pests to successfully invade and dominate in interactions with other species. In this study, we report that Cydia pomonella (Linnaeus), a notorious invasive pest of pome fruits and walnuts globally, employs unique reproductive strategies in response to quercetin, a plant compound in host fruits. By monitoring adult dynamics and fruit infestation rates, we observed a competitive relationship between C. pomonella and the native species Grapholita molesta (Busck) . C. pomonella was able to occupy vacant niches to ensure its population growth. We also found that quercetin had different effects on the reproductive capacity and population growth of C. pomonella and G. molesta . While quercetin stimulated the fecundity and population growth of G. molesta , it inhibited C. pomonella . However, C. pomonella was able to rapidly increase its population after exposure to quercetin by adopting an ‘accelerated burst’ of oviposition strategy, with each individual making a greater reproductive contribution compared to the control. We further demonstrated that the effect of quercetin on oviposition is regulated by the juvenile hormone (JH) signaling pathway in C. pomonella , allowing it to prioritize survival. The enhanced reproductive fitness of G. molesta in response to quercetin is attributed to the regulation of JH titers and key genes such as Met and Kr-h1 , which in turn up-regulate reproduction-related genes Vg and VgR. In contrast, C. pomonella is inhibited. These findings shed light on the mechanisms interspecific competition and help to improve our understanding of the global spread of C. pomonella , which can be attributed to its inherent superiority in terms of reproductive strategy.
研究领域:
昆虫学
·
生态学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Chao Hu,Zi-Han Wei,Pei-Rong Li,James D. Harwood,Xiang-Yang Li,Xue-Qing Yang
The codling moth, Cydia pomonella (L.), is a quarantine pest of global significance impacting pome fruits and walnuts. It has evolved resistance to many commonly used insecticides including λ-cyhalothrin. Glutathione S-transferases (GSTs) are multifunctional enzymes playing a crucial role in the detoxification of insecticides in insects. However, the role of specific GST gene in λ-cyhalothrin resistance in C. pomonella is unclear. In this study, we identified three sigma-class genes (CpGSTs1, CpGSTs2, and CpGSTs3). These genes were ubiquitously expressed at all developmental stages, and of these, the expression level of CpGSTs2 in the larval stage was significantly higher than in the egg, pupal, and adult stages. Moreover, CpGSTs2 was predominantly expressed in the fat body while lower levels in the cuticle. In addition to exposure of larvae to LD10 of λ-cyhalothrin elevating the expression level of CpGSTs2, mRNA levels of CpGSTs2 in a field population (ZW_R) from northeast China, which has developed moderate level resistance to λ-cyhalothrin, was significantly higher than that of susceptible strains. In vitro inhibition assays demonstrated that λ-cyhalothrin inhibited the conjugating activities of recombinant CpGSTs2, and metabolic assays indicated that λ-cyhalothrin could be depleted by recombinant CpGSTs2. These results bring evidence for the involvement of CpGSTs2 in C. pomonella in resistance to λ-cyhalothrin.
研究领域:
昆虫学
·
农药毒理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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