ACS Applied Materials & Interfaces
·
2015
·
IF 6.723
Yanning Qu,Renliang Huang,Wei Qi,Rongxin Su,Zhimin He
Colloidosomes are promising carriers for immobilizing enzyme for catalytic purposes in aqueous/organic media. However, they often suffer from one or more problems regarding catalytic performance, stability, and recyclability. Here, we report a novel approach for the synthesis of cross-linkable colloidosomes by the selective polymerization of dopamine at oil/water interfaces in a Pickering emulsion. An efficient enzyme immobilization method was further developed by covalently bonding enzymes to the polydopamine (PDA) layer along with the formation of such colloidosomes with lipase as a model enzyme. In this enzyme system, the PDA layer served as a cross-linking layer and enzyme support for simultaneously enhancing the colloidosomes’ stability and improving surface availability of the enzymes for catalytic reaction. It was found that the specific activity of lipases immobilized on the colloidosome shells was 8 and 1.4 times higher than that of free lipase and encapsulated lipase positioned in the aqueous cores of colloidosomes, respectively. Moreover, the immobilized lipases demonstrated excellent operational stability and recyclability, retaining 86.6% of enzyme activity after 15 cycles. It is therefore reasonable to expect that this novel approach for enzyme immobilization has great potential to serve as an important technique for the construction of biocatalytic systems.
研究领域:
材料科学
·
生物化学
·
催化工程
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DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2026
·
IF 9.6
Qi Zeng,Dingyi Yang,Ting Guo,Haoyue Hou,Xingyuan Fang,Nan Xue,Hao Yuan,Hengquan Yang,Tao Meng
Tuning SiO 2 nanoparticle size creates uniform precise nanopores matching enzymes within colloidosome shells. • Hollow porous colloidosomes provide high-density enzyme sites, yielding dozens-fold higher efficiency than free CALB in batch. • Integrating CALB-colloidosomes into a continuous-flow reactor yields 21.3-fold efficiency and >85% stability after 200 h. Enzyme confinement at the molecular scale reveals catalytic performance that is previously unseen at the ensemble level, but all such delicate enzyme hosts now available encounter their intrinsic issues. Here, we present a mild, one-pot emulsification-directing approach to customize colloidosomes (CSs) with pores matching enzyme size as enzyme hosts for optimizing continuous-flow biocatalysis. By means of the dissolution-induced inside-out self-assembly, uniform and enzyme-matched pores can be readily achieved. Typically, 5.8-nm pores obtained using 22-nm SiO 2 nanoparticles (SiO 2 NPs) as building blocks are optimal for confining Candida antarctica lipase B (CALB, ∼5 nm), conferring 98.3% activity recovery and minimal leaching of only 2.1%. The catalytic efficiency of CALB-confined colloidosomes (CALB/CSs) is dozens of times higher than that of free CALB (fCALB) in batch mode. Moreover, a 21.3-fold catalytic efficiency was achieved by integrating robust CALB/CSs with a continuous-flow platform. Meanwhile, the desired long-term stability was confirmed, with over 85% of the initial efficiency maintained after 200-h of consecutive operation. This study offers a potentially generalizable strategy that establishes a CS platform as an enzyme-confined host that is competent for continuous flow manufacturing, hoping to unlock the full potential of enzymatic reactions. Customizable colloidosomes with uniform enzyme-matched pores are fabricated via a mild, one-pot emulsification-directed self-assembly. The colloidosome shell is composed of hexagonal close-packed SiO 2 nanoparticles that form uniform pores, whose size is easily tuned by the size of the SiO 2 nanoparticles and thus precisely customizable for target enzymes. The robust colloidosome hosts exhibit efficient catalytic efficiency and long-term stability in continuous-flow reactors. Download: Download high-res image (162KB) Download: Download full-size image
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DOI
PubMed
高影响因子
COLLOID AND POLYMER SCIENCE
·
2019
·Vol. 297
·期 10
·1301-1311
·
IF 1.906
Liu Pin,Qi Chenze,Gao Yong
Colloidosomes have gained intensive attention in the past years due to their substantial applications in wide scopes involving encapsulation, catalysis, and drug delivery, etc. In this study, we depicted the templated fabrication of well-shaped colloidosomes with tunable shell thickness by Pickering emulsion at a mild condition. To this end, toluene-in-water Pickering emulsion stabilized by cetyltrimethylammonium bromide (CTAB)–modified polydopamine (PDA) nanoparticles (PDA@CTAB) was prepared. After the covalent cross-linking of PDA nanoparticles assembled on the surface of an oil droplet through chemical reactions occurring in toluene phase, exclusive intra-colloidosomes were afforded. The shell thickness of colloidsomes could be roughly tuned by the adjustment of the amount of the reactive monomers in the oil phase. Our investigations revealed that CTAB was critical for the successful fabrication of well-shaped colloidosomes. Otherwise, only irregular structures were obtained if pristine PDA–stabilized Pickering emulsion was applied. The current strategy could enable hydrophobic cargo, such as 10-hydroxycamptothecin to be encapsulated while colloidosomes were created. The release behavior of the encapsulated cargoes was also investigated.
CTAB was necessary for the templated fabrication of well-shaped colloidosomes by PDA-stabilized oil-in-water Pickering emulsion
研究领域:
材料科学
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药物递送
·
化学工程
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DOI
高影响因子
APPLIED CLAY SCIENCE
·
2017
·
IF 3.101
Sen Lin,Shiyong Sun,Kexuan Shen,Daoyong Tan,Hongping Zhang,Faqin Dong,Xiaoxin Fu
Photocatalytic microcompartments have attracted increasing interests in the field of photocatalytic degradation . This work focused on the construction and characterization of a novel photocatalytic microsystem comprising polydopamine-modified TiO 2 nanoparticles (NPs) dispersed in clay-based colloidosomes, utilizing partially hydrophobic montmorillonite particles as building blocks for colloidosome membrane assembly. Assessment of photocatalytic activity was performed by photocatalytic degradation of methylene blue and rhodamine B, with the small molecule dyes decomposing within the compartmentalized microsystems. Encapsulation of TiO 2 NPs within the clay colloidosomes enhanced the efficiency of photocatalytic degradation. The results showed a selective degradation of methylene blue in the presence of rhodamine B, with associated λ MB /λ RhB of 2.04. The present study indicates that photocatalytic semiconductor NPs encapsulated in clay colloidosomes form a promising microsystem with great potential for application in environmental remediation.
研究领域:
环境科学
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材料科学
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化学工程
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产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2026
·
IF 13.2
Zoe Sullivan Atkins,Peilong Li,Leila Khazdooz,Amin Zarei,Alireza Abbaspourrad
Advances in modified starches are needed to meet consumer and regulatory demands for label friendly, resilient thickeners. Starch-based patchy charged colloidosomes (PCCs) thicken uniquely through a combination of mechanisms providing an advantage over other modified starches. PCCs are hollow spheres composed of positively charged and negatively charged starch granules. PCCs were fabricated in three ratios of positive to negative starch granules: 1:9, 3:7, and 1:1. A positive colloidosome and a negative colloidosome were fabricated as non-patchy controls. Negatively charged starch granules were extracted from amaranth flour and positively charged starch was obtained by chemical modification with 2,3-epoxypropyltrimethylammonium chloride (ETMAC). PCCs were fabricated using a scalable, bottom-up, oil in water emulsion template. The physical arrangement of starch granules into hollow, spherical structures (~17–90 μm in diameter) increased solution viscosity through specific volume increase (4–7×). The PCCs were water-permeable and solutions thickened as the continuous phase decreased. In combination with the structural thickening, attraction between the positive and negative charges in the starch colloidosomes further contributed to enhanced viscosity. The hollow arrangement increased viscosity by 3–10× depending on charge ratio. The advantage of PCCs lies in their multi-mechanism thickening that leverages structure and electrostatic attractions to improve resilience to external stresses.
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DOI
高影响因子
Science China-Chemistry
·
2020
·Vol. 63
·期 10
·1507-1514
·
IF 6.356
Yin Kun,Zeng Xi,Liang Xuan,Wei Haopai,Zeng Huimin,Qi Wei,Ruan Weidong,Song Yanling,Yang Chaoyong,Zhu Zhi
Droplet microfluidics is a powerful platform for high-throughput single-molecule protein analysis. However, the issues of coalescence and crosstalk of droplets compromise the accuracy of detection and hinder its wide application. To address these limitations, a novel colloidosome-based method was presented by combining a Pickering emulsion with droplet microfluidics for single-molecule protein analysis. Utilizing the self-assembly of easily synthesized colloidal surfactant F-SiO 2 NPs at the water/oil interface, the colloidosomes are rigidly stabilized and can effectively avoid the leakage of fluorescent molecules. The crosstalk-free colloidosomes enable high-throughput single-molecule protein analysis, including heterogenous dynamic studies and digital detection. As a robust and accurate method, colloidosome-based microfluidics is promising as a powerful tool for a wide variety of applications, such as directed enzyme evolution, digital enzyme-linked immunosorbent assay (ELISA), and screening of antibiotics.
研究领域:
生物技术
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分析化学
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材料科学
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DOI
高影响因子
AICHE JOURNAL
·
2024
·
IF 3.5
Rong-Kun Liu,Jia Jia,Qian Sun,Hong Zhao,Jie-Xin Wang
The colloidosome, as a kind of Pickering microcapsule, offers a highly functionalizable approach for encapsulation, but current studies mainly focus on shells that lack real-time modulated permeability. Herein, a scalable spray drying method was employed to fabricate temperature/near-infrared (NIR)/magnetic multi-stimuli responsive colloidosomes with adjustable permeability, facilitating intelligent transmembrane transport of molecules. The colloidosomes assembled with SiO 2 /polydopamine/poly (N-isopropyl acrylamide) nanoparticles can be heated from 25°C to 50°C within 5 min upon NIR exposure. The permeability of shells regulated by temperature and NIR allows for real-time remote control. Yeast cells, as an example, are encapsulated within colloidosomes. The decomposition rate of H 2 O 2 varies with temperature (25–45°C) and NIR intensity (0.5–1 W cm −2 ), which can attain a more than sixfold increase in the rate constant of pseudo-first-order kinetic model. Furthermore, the strategy was expanded by encapsulating doxorubicin/UiO-66 particles, highlighting its great potential in realms of multi-stimuli responsive colloidosomes applications.
研究领域:
材料科学
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化学工程
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生物技术
·
药学
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DOI
高影响因子
Inorganic Chemistry Frontiers
·
2024
·
IF 6.1
Chengkun Bai,Bingdi Wang,Zhengshun Jiang,Chunying Lv,Zhenning Liu,Shiyu Wang,Song Liang,Hongying Zang
Inspired by the ordered processes of chloroplasts in photosynthesis involving coordinative linkages and efficient compartmentalization, a bioinspired compartmentalized photocatalytic system (BCPS) with an inorganic compartment is designed for photocatalytic hydrogen evolution with light driven electron carrier mediated cross-membrane energy transport. A typical BCPS comprises silica colloidosomes containing co-catalysts as microreactors, where positively charged N-methyl-4-cyanopyridinium (MCP+) serves as the electron carrier. The MCP+ molecules harvest energy from photoexcited g-C3N4, and transit to electrically neutral species MCP0, which can pass the colloidosome. Driven by light, the MCP0 diffuse across the membrane and transport electrons to H+ for hydrogen evolution, in which Pt particles are employed as co-catalysts. Analogous to natural photosynthesis, hydrogen evolution occurs within compartments, separating light and dark reactions, protecting the dark reaction from interference during light harvesting and minimizing potential side effects. The system enables sustained evolution of hydrogen with a higher yield than that with the control catalysis in a bulk catalyst suspension. The dramatic influence of SDS on the hydrogen evolution was revealed, and a possible mechanism of electron carrier amount buffering was proposed.
研究领域:
化学
·
材料科学
·
仿生学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Jia Jia,Rong-Kun Liu,Qian Sun,Jie-Xin Wang
ZnO nanoparticles with high safety and stability are often used as active ingredients in sunscreens to protect the skin from ultraviolet rays. However, ZnO nanoparticles are easy to agglomerate, which will significantly affect the ultraviolet absorption and bacteriostatic properties, and the reactive oxygen species induced by the photocatalytic activity may result in irreversible secondary damage to the skin. Herein, the ZnO nanoparticles are dispersed uniformly on the surface of latex particles, and these composite particles are used as shell materials to construct self-assembled colloidosomes by high-gravity technology, which can improve the application properties with synergistic enrichment of the hollow structure. The ultraviolet resistance of colloidosomes is significantly higher than that of the pure ZnO nanoparticles. The higher the loading capacity, the more obvious the inhibition effect of colloidosomes on the growth of Gram bacteria. Furthermore, the antioxidant anthocyanin is in situ encapsulated in colloidosomes, and at a concentration of 2 g/L, the high free radical scavenging rate of 78% can be achieved. The construction of multifunctional colloidosomes provides a route for sunscreen and cosmetics applications.
研究领域:
材料科学
·
生物学
·
化妆品科学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2020
·
IF 3.573
Rong-Kun Liu,Ting-Ting Hu,Jia Jia,Dan-Lei Yang,Qian Sun,Jie-Xin Wang,Jian-Feng Chen
Colloidosomes have been extensively studied in recent years because of their potential applications in various fields, such as food, cosmetics, or pharmaceutical industries. Herein, we present a simple and highly efficient route for large-scale preparation of colloidosomes with controllable permeability using spray drying technology. By using the latex particles with different glass transition temperatures, the colloidosomes with uniform and controllable shell porosities can be easily constructed. The effects of latex particle concentrations, flow rates of compressed air, inlet temperatures, and feed rates on the morphologies of colloidosomes were explored in detail, and the possible formation mechanism was given. Both the feed rates and inlet temperatures can affect the evaporation rate of solvents. Only the preparation conditions falling on the suitable speed of the solvent evaporation “area” can result in complete colloidosomes. In addition, the Congo red dye was used as a model for encapsulation and controllable release. The release duration of colloidosomes prepared with different Tg latex particles ranged from 23 to 52 h as the permeability drops. The results demonstrate that this colloidosome fabrication method is feasible for continuous large-scale production and precisely controllable release for practical applications.
研究领域:
材料科学
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化学工程
·
药学
·
食品科学
·
化妆品
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Xiujuan Han,Hongfang Zhang,Jianbin Zheng
Colloidosome is a novel nanostructure composed of millions of colloid particles. In this work, nanosized PbS colloidosomes were initially prepared and applied as nanoprobes for an ultrasensitive immunoassay. The colloidosomes were simply prepared in mild conditions by assembling the elementary approximately 8 nm PbS nanoparticles at the water-in-oil interface of emulsion droplets. To enhance the rigidity and biocompatibility of the colloidosomes, interfacial polymer was introduced by utilizing self-polymerization of dopamine. By treating with dilute nitric acid, a bursting release of lead ions from the colloidosomes occurred and the lead ions can be detected easily by anodic stripping voltammetry. In this way, a colloidosome-based electrochemical immunoassay was developed by using the nanosized PbS colloidosomes as electroactive labels. The proposed method featured a linear calibration range from 10 fg·mL–1 to 100 ng·mL–1 with a low detection limit of 3.4 fg·mL–1 for the detection of human epididymis protein 4. This work introduced a new member for the family of colloidosomes and offered a novel perspective for the rational implementation of various colloidosomes for novel low-abundance cancer biomarkers analysis.
研究领域:
纳米技术
·
分析化学
·
生物标志物检测
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2013
·
IF 5.008
Yi Gong,Ai Mei Zhu,Qiu Gen Zhang,Mei Ling Ye,Hai Tao Wang,Qing Lin Liu
Cell encapsulation by locking the interfacial microgels in a water-in-oil Pickering emulsion has currently been attracting intensive attention because of the biofriendly reaction condition. Various kinds of functional microgels can only stabilize an oil-in-water Pickering emulsion, and it is thus difficult to encapsulate cells in the emulsion where the cells are usually dispersed in the continuous phase. Herein, we introduce a facile method for preparing cell-embedded colloidosomes in an oil-in-water emulsion via polyelectrolyte complexation. Escherichia coli (E. coli) was chosen as a model cell and embedded in the thin shell of chitosan/poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-co-AAc)) microcapsules. This is beneficial for expressing cell function because of the little resistance of mass exchange between the embedded cells and the external environment. Cells can be used in biocatalysis or biomedicine and our product will hold great promises to improve the performance in those fields. The synthesis route presents a platform to prepare cell-embedded microcapsules in an oil-in-water Pickering emulsion in a facile and biocompatible way. First, an emulsion stabilized by P(NIPAM-co-AAc) microgels was prepared. Then, the interfacial microgels in the emulsion were locked by chitosan to form colloidosomes. The mechanism of cell encapsulation in this system was studied via fluorescent labeling. The viability of E. coli after encapsulation is ca. 90%. Encapsulated E. coli is able to metabolize glucose from solution, and exhibits a slower rate than free E. coli. This demonstrates a diffusion constraint through the colloidosome shell.
研究领域:
生物技术
·
材料科学
·
化学工程
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产品关联
DOI
PubMed
高影响因子
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