JOURNAL OF BIOMATERIALS APPLICATIONS
·
2017
·
IF 2.31
Jianye Wei,Wei Xue,Xifei Yu,Xiaozhong Qiu,Zonghua Liu
In the field of immunotherapy, immune vaccinations have received more and more attention for disease prevention and treatment. In immune vaccination, efficient vaccine adjuvants are necessary due to the weak immunogenicity of vaccines. Some traditional vaccine adjuvants have been widely used but have shown obvious limitations such as poor biosafety. Therefore, researchers make a great effort to develop more functional novel immune adjuvants such as chitosan-based immune adjuvants. However, chitosan is poorly water soluble, which greatly limits its application as immune adjuvants, regardless of its good biocompatibility, biodegradability, and other biological activities. In this work, we prepared a water-soluble chitosan derivative phosphorylated chitosan (PCS) and evaluated its potential as a novel immune adjuvant. PCS was found to be pH sensitive: specifically, it was water soluble at pH < 7.0 but began to gel at pH >7.0. By virtue of this, neutral PCS aqueous solutions containing ovalbumin (OVA) antigen was intramuscularly injected into test mice, which would transform to an OVA-containing gel network for OVA immunization. The results showed that the use of 30 mg/mL PCS-based hydrogel as vaccine delivery system contributed to significantly higher level of antigen-specific immune responses, including higher level of antigen-specific IgG antibodies, IFN-γ and IL-4 cytokines secretion by splenocytes, as well as memory CD4+ and CD8+ T cells. In vivo imaging and immunohistochemistry assays suggest that the improved immunization efficacy may be attributed to the controlled release of antigen from injection site by PCS gel network, and then prolonged antigen stimuli to the immune system. From the results, PCS could be developed as a promising vaccine delivery system for immunotherapy.
研究领域:
免疫学
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生物材料学
·
疫苗学
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DOI
PubMed
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2017
·Vol. 24
·期 1
·212-221
·
IF 5.317
Jun Song,Yanlong Wei,Jiwen Hu,Guojun Liu,Zhengzhu Huang,Shudong Lin,Feng Liu,Yangmiao Mo,Yuanyuan Tu,Ming Ou
Graphical Gated delivery : pH-Responsive porous nanocapsules have been prepared from ternary graft copolymers and used as potential vehicles for controlled release of drugs, food, and bioactive compounds (see figure). In this work pH-responsive porous nanocapsules have been successfully prepared from a ternary graft copolymer, poly(glycidyl methacrylate)- g -[poly(2-cinnamoyloxyethyl methacrylate)- r -poly(ethylene glycol) methyl ether- r -poly(2-diethylaminoethyl methacrylate)] or PGMA- g -(PCEMA- r -MPEG- r -PDEAEMA). The graft copolymers were fabricated by grafting three types of polymer chains onto the backbone polymer by using click chemistry. These ternary copolymers underwent self-assembly to form vesicles in a DMF/water solvent mixture. While the MPEG chains served as the corona and stabilized the vesicles, the vesicle wall was composed of a dominant PCEMA continuous phase that was interspersed by PDEAEMA domains. After photo-cross-linking, the PDEAEMA domains were embedded in the structurally locked PCEMA wall. By decreasing the pH of the external solution, we were able to trigger the release of encapsulated pyrene due to the capsule wall becoming porous as a result of the PDEAEMA chains bearing positively charged amine groups stretching into the water. While these pH-responsive porous nanocapsules exhibited potential applications in drug delivery, detection and catalysis, the strategy reported in this contribution also represented a new paradigm for the design and preparation of other novel stimuli-responsive porous nanocapsules.
研究领域:
材料科学
·
药学
·
生物医学工程
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DOI
PubMed
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2016
·Vol. 23
·期 8
·156
·
IF 1.969
Zhang Wu,Ren Guangli,Xu Hao,Zhang Jinglin,Liu Huidi,Mu Shansong,Cai Xiang,Wu Ting
To realize the controlled release property, lower cytotoxicity, and long-term bioactivity of tetracycline, the chitosan hydrogel had been prepared using genipin as the cross-linker and the tetracycline was in situ encapsulated. The formation process and strength of hydrogel were studied by rheological analysis, and the microtopography was observed by scanning electron microscope. It was found that the amount of genipin could modulate the physical property of the hydrogel. For in vitro release and antibacterial assays, the hydrogel could controllably release tetracycline and keep its bioactivity for a long time. The tetracycline-loaded chitosan hydrogel showed good antibacterial effect even under alkaline environment, which displayed better stability compared with free tetracycline. Moreover, the tetracycline-loaded chitosan hydrogel exhibited lower cytotoxicity than did tetracycline alone, suggesting that this tetracycline-loaded chitosan hydrogel could be a more useful dosage form than separate doses of tetracycline. The novel aspects of this study include the cytotoxicity study and the in vitro and in vivo assays, which might be useful for other researchers in this field.
To realize the controlled release property, lower cytotoxicity, and long-term bioactivity of tetracycline, genipin cross-linked chitosan hydrogel was used as the carrier of tetracycline. The hydrogel could controllably release tetracycline with bioactivity. The hydrogel showed good antibacterial effect even under alkaline environment. The hydrogel exhibited lower cytotoxicity than did tetracycline alone.
研究领域:
药剂学
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生物材料
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药物递送
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组织工程
·
抗菌治疗
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DOI
高影响因子
JOURNAL OF THE TEXTILE INSTITUTE
·
2023
·
IF 1.7
Yifan Huang,Lei Wang,Liu Yi,Binjie Xin,Tingxiao Li
Absorbing exudate and realizing drug - controlled release are considered to be the ideal objectives and effects of wound dressing. Therefore, multi - layer wound dressing is regarded as to be a common means for treating burns and other wounds with more exudate in skin tissue engineering. We report a unique platform based on multilayer nanofiber membrane, which can simultaneously inhibit bacterial breeding and prevent wound infection. An electrospun fiber pad containing tetracycline hydrochloride (TCH) was prepared as the core layer, and a layer of polyethylene oxide (PEO)/sodium alginate (SA) nanofiber membrane was covered on the upper and lower layers. The average diameter of multilayer nanofiber membrane is 440 nm, which has good biocompatibility and excellent antibacterial effect. Due to the regular arrangement of nanofibers and more small voids, it is conducive to cell adhesion and proliferation, and the drug in the core layer of fiber membrane has a relatively long release time. Through the bacteriostatic circle experiment, it has a good effect on inhibiting the growth and invasion of bacteria.
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DOI
高影响因子
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES
·
2019
·
IF 1.463
Qi Wang,Mengfei Shen,Wenjing Li,Wanwan Li,Fang Zhang
Pesticides are biological or chemical substances used to manage pests and diseases. Encapsulation of pesticides in biodegradable carriers creates a slow-release system that can improve water dispersibility and prolong residual activity. We prepared two kinds of poly (lactic-co-glycolic acid)(PLGA) nanoparticles (NPs) with polyvinyl alcohol (PVA) and sodium dodecyl sulfate (SDS) surfactants. These were used to encapsulate the fungicide fluazinam (Flu) against Rhizoctonia solani using the Shirasu Porous Glass (SPG) membrane emulsification method. Both nanoparticles had uniform spherical shapes with average diameters of 314.13 nm (SDS) and 612.80 nm (PVA). The slow-release microspheres had excellent sustained-release properties, resistance to UV degradation, storage stability, leaf surface coverage and antifungal efficacy compared to the commercial formulation.
研究领域:
农业科学
·
材料科学
·
药学
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DOI
PubMed
高影响因子
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2019
·Vol. 104
·期 1
·149-155
·
IF 1.65
Xie Yan-Li,Jiang Wayne,Li Fen,Zhang Yu,Liang Xiao-Yu,Wang Meng,Zhou Xueqing,Wu Shao-Ying,Zhang Cheng-Hui
This study was intended to develop an environment-friendly controlled release system for spirotetramat in an alginate matrix. Four formulations, starch–chitosan–calcium alginate (SCCA), starch–calcium alginate (SCA), chitosan–calcium alginate (CCA), and calcium alginate (CA) complex gel beads, were prepared by the extrusion–exogenous gelation method. The properties of the formulations were studied. The results showed that the release behaviors of the formulations in water could be well described by the logistic model, and the release occurred through Fickian diffusion. Among the four formulations, SCCA showed the highest entrapment efficiency, drug loading and the slowest release rate. Degradation studies revealed that the SCCA formulation exhibited an obvious slower degradation rate of spirotetramat in soils than the commercially available formulation. The estimated half-life of the SCCA formulation was 2.31, 3.25, and 4.51 days in waterloggogenic paddy soil, purplish soil, and montmorillonite, respectively, when the soils were moistened to 60% of its dry weight. This study provided a possible approach to prolong the duration of spirotetramat and to reduce environmental contamination.
研究领域:
农药科学
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环境科学
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材料科学
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DOI
PubMed
高影响因子
JOURNAL OF INCLUSION PHENOMENA AND MACROCYCLIC CHEMISTRY
·
2011
·Vol. 71
·期 3
·593-602
·
IF 1.22
Zhang Jun,Yu Meihua,Yuan Pei,Lu Gaoqing,Yu Chengzhong
In this work, a series of nanoporous silica materials have been prepared as adsorbents for volatile (−)-menthol, a molecule widely used in food, pharmacy, and cosmetics. The isothermal release properties of (−)-menthol have been investigated and correlated with the structural parameters of nanoporous absorbents. A rotary evaporation method is used to effectively load (−)-menthol into the nanopores of adsorbents and to prevent the whisker growth during the adsorption. It is demonstrated that the pore size, structure, wall thickness and surface functionality of nanoporous adsorbents are four important parameters to influence the isothermal release of (−)-menthol. By tuning these parameters of nanoporous silica adsorbents, controlled release of (−)-menthol can be achieved. A vesicular silica material with thick wall and hydrophobic functional groups is shown to possess the slowest release performance. Our contribution provides important knowledge for the future applications of nanoporous silica materials in pharmacy and cosmetics.
研究领域:
药学
·
化妆品科学
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材料科学
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DOI
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 11
·2089
·
IF 3.06
Xuexia Lin,Qiaoqiao Ma,Jianlong Su,Cui Wang,Ranjith Kumar Kankala,Mingrong Zeng,Honggui Lin,Shu-Feng Zhou
In this work, with the drug oxytetracycline (OTC) released, cell cytotoxicity and antimicrobial studies of dual-responsive sodium alginate andN-Isopropylacrylamide hydrogels (SA/pNIPAAm) with enclosed OTC were investigated. The molecular OTC release was explored with different acid-base conditions and temperature conditions. In order to characterize cell cytotoxicity and antimicrobial efficacy, time-dependent OTC release analysis of different acid-base conditions was performed in SA/pNIPAAm hydrogels.[email protected]/pNIPAAm hydrogels showed excellent time-dependent antimicrobial efficacy, in which the IC50 values were 50.11 μg mL−1, 34.27 μg mL−1, and 22.39 μg mL−1among three consecutive days, respectively. Meanwhile, the human cells showed excellent viability at the IC50 dosage of[email protected]/pNIPAAm (50.11 μg mL−1).[email protected]/pNIPAAm performed in this study indicated that SA/pNIPAAm may serve as drug carriers for sustainable release at a specific concentration and for being employed as substrates for decreasing drug toxicity. Besides, pH-responsive and thermos-responsive SA/pNIPAAm may lead to the better selectivity of drug release in the ideal location or site. Finally, the results demonstrate that the designed, dual-responsive, biocompatible[email protected]/pNIPAAm hydrogels showed excellent antimicrobial efficacy and may potentially be found to have enormous applicability in the field of pharmaceutics.Keywords:pH-responsive;thermos-responsive;drug release;antimicrobial efficacy;drug carrier
研究领域:
药剂学
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生物材料
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Kailun Chen,Hui Zhang
Biopolymer aerogel microspheres based on alginate and pectin, were fabricated by crosslinking with divalent cation (Ca 2+ ) and sol-gel method followed by freeze-drying process. The morphology of the alginate/pectin aerogel microspheres examined by scanning electron microscopy indicated that the porosity and pore size increased with the increasing pectin ratio in the aerogels. The aerogels with higher alginate content presented higher stiffness, while the aerogels with higher pectin ratio exhibited the significantly ( p < 0.05) higher water solubility and equilibrium moisture. Furthermore, proanthocyanidins were encapsulated in these aerogel microspheres and showed a good controlled release, as well described by first-order and Korsmeyer-Peppas models. The radical scavenging activity and ferric reducing antioxidant power results indicated that the encapsulated aerogel microspheres with higher pectin content had a stronger antioxidant activity .
研究领域:
食品科学
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材料科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
INTEGRATED FERROELECTRICS
·
2015
·
IF 0.357
Yu Guo Zhuo,Jun Liu,Qing Shan Li,Guang Zhong Xing,Wen Feng Lv
Na-CMC polyurethane microspheres were prepared with isophorone diisocyanate and polyethylene glycol and sodium carboxymethyl cellulose as main materials. Ibuprofen used as a model drug and core material was coated into microspheres. The microspheres morphologies and the composite physical states were investigated using optical microscopy, scanning electron microscopy (SEM), differential scanning calorimetry (DSC) and Fourier transform infrared (FTIR) spectroscopy. Optical microscopy and scanning electron microscopy observations revealed that had a good spherical structure. Results from FTIR and DSC suggested that the Na-CMC had been successfully introduced to the polyurethane molecular chain, Na-CMC polyurethane microspheres had good thermal stability, and heating weightlessness was less than 2% in the 300°C. Na-CMC polyurethane microspheres carried drug were pH sensive and the drug release rate under different pH value were different.
研究领域:
药学
·
材料科学
·
药物递送系统
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产品关联
DOI
高影响因子
CHEMBIOCHEM
·
2024
·
IF 2.6
Wen Fu,Zhongli Shao,Zhiping Xu,Zhong Li,Xusheng Shao
Graphical NP-TBZ is developed by covalently linking o -nitrobenzyl ( NP ) with thiabendazole ( TBZ ). Compound NP-TBZ can be controlled to release TBZ in dependent to light, which resulted in controllable in-vivo activity against wheat scab. It is promising for spatiotemporal controlled-release of TBZ in factual applications, posing a theoretical guidance in development of controlled-release pesticides. Pesticides are essential in agricultural development. Controlled-release pesticides have attracted great attentions. Base on a principle of spatiotemporal selectivity, we extended the photoremovable protective group (PRPG) into agrochemical agents to achieve controllable release of active ingredients. Herein, we obtained NP-TBZ by covalently linking o -nitrobenzyl ( NP ) with thiabendazole ( TBZ ). Compound NP-TBZ can be controlled to release TBZ in dependent to light. The irradiated and unirradiated NP-TBZ showed significant differences on fungicidal activities both in vitro and in vivo. In addition, the irradiated NP-TBZ displayed similar antifungal activities to the directly-used TBZ , indicating a factual applicability in controllable release of TBZ . Furthermore, we explored the action mode and microcosmic variations by SEM analysis, and demonstrated that the irradiated NP-TBZ retained a same action mode with TBZ against mycelia growth.
研究领域:
农业科学
·
植物保护
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产品关联
DOI
PubMed
高影响因子
iScience
·
2026
·
IF 4.5
Xiaochen Su,Weitao Zhao,Lulu Wang,Panpan Song,Xingbo Wang,Xuefei Jin,Haiyuan Zhang
Melanoma, the most lethal form of skin cancer, remains a significant therapeutic challenge despite advances in immunotherapy. Although PD-1/PD-L1 blockade improves clinical outcomes, its effectiveness is frequently limited by suboptimal response rates and treatment resistance. Here, we developed a novel strategy targeting iron-dependent PD-L1 regulation. Since elevated iron activates PI3K/AKT signaling and upregulates PD-L1, we employed the iron chelator deferasirox (DFX) to disrupt this pathway. To overcome DFX’s poor solubility and bioavailability, we engineered tumor-targeting, glutathione-responsive albumin nanoparticles modified with cRGD peptides ( RGD AB@DFX NPs). These NPs selectively accumulated in B16F1 melanoma tumors and released DFX in response to intracellular glutathione, effectively downregulating PD-L1. These findings suggest that iron modulation represents a promising approach to enhance immunotherapy efficacy, with RGD AB@DFX NPs serving as an optimized delivery platform for clinical application.
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高影响因子
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