Journal of Analytical Science and Technology
·
2021
·Vol. 12
·期 1
·1-8
·
IF 2.769
Li Xiangfei,Qiao Juan,Sun Yongjun,Li Zhiwei,Qi Li
Precisely changing the optical properties of gold nanoclusters (AuNCs) with different ligands offers a promising prospect for highly sensitive and selective drug sensing. In this study, AuNCs were synthesized with d-tryptophan (d-Trp) and its derivatives as the ligands. Optical measurements showed that d-Trp@AuNCs produced higher fluorescence intensity and shorter fluorescence emission wavelength than the d-Trp-derivatives-ligands protected AuNCs, indicating that the ligand-shell rigidity and core-shell charge transfer affected their fluorescent properties. At the excitation wavelength of 370 nm, the emission wavelength of d-Trp@AuNCs was 460 nm. The fluorescence changes revealed the high selectivity of d-Trp@AuNCs for detecting folic acid due to the static quenching and inner filter effect. In the presence of folic acid, the fluorescence of d-Trp@AuNCs was remarkably quenched with good linearity ranging from 6.3-100.0 μM (R2 = 0.997) and a detection limit of 5.8 μM. The proposed assay was successfully utilized to determine the amount of folic acid in human urine with recoveries from 94.3 to 107.3%. This work shows the great potential of d-Trp@AuNCs for detecting folic acid in real bio-samples. It also presents an effective strategy for preparation of the AuNCs with enhanced fluorescence efficiency by regulating the rigidity of the ligands shell and the core-shell charge transfer.
研究领域:
分析化学
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生物医学工程
·
纳米科学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2016
·
IF 5.31
Zhiyong Zhou,Lichao He,Yu Mao,Wenshuai Chai,Zhongqi Ren
Enantioseparation of racemic tryptophan is very important for pharmaceutical production. Conventional separation methods are high costly, energy intensive and environmentally unfriendly. Permeation separation based on membrane separation technique is a promising technology with advantages of energy efficient, clean and no additives. However, lots of organic compounds are commonly used for the traditional preparation of various membranes for enantioseparation of racemic tryptophan. Thus, we developed a totally green and clean preparation method using nature polymer sodium alginate as functional polymer, water as solvent and CaCl 2 as crosslinking agent. Besides, polyvinylidene fluoride membrane was selected as supported membrane. Molecularly imprinted technology with d -Tryptophan as template molecule was introduced to enlarge mass transfer flux. Various methods were used in characterization of molecularly imprinted composite membrane (MICM) and non-imprinted composite membrane (NICM). Effects of preparing conditions, permeation conditions and concentration polarization on permeation performance of MICM by pressure-driven were investigated. High enantiomeric excess ( ee % > 98%) of the permeation solution could be obtained under mild conditions. Reducing the presence of concentration polarization was beneficial to maintaining high ee % (>98%). Moreover, the thermo and chemical stability of MICM were studied too. Comparison with previous studies indicated that the proposed totally green and clean method was not only low costly and environment friendly but also beneficial to significantly increasing ee .
研究领域:
生物化学
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制药工程
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材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF LIGHTWAVE TECHNOLOGY
·
2025
·
IF 4.8
Xiu Du,Lingxin Kong,Mingxing Liu,Yu Zhong,Kuihua Ding
In this paper, a fiber optic tryptophan enantiomers chiral sensor based on metal-organic frameworks (MOFs) is proposed. Two kinds of MOFs with different chiral microenvironments were coated on the surface of U-shaped sensor and helical long-period grating (HLPG), and the two sensors achieved specific identification and quantitative sensing of L-Trpptophan (L-Trp) and D-Trpptophan (D-Trp) in racemic mixture solution. The two MOFs have different adsorption effects on L-Trp and D-Trp, which makes the two sensors have different sensitivity. The results showed that the sensitivity of the sensor was -24.34 nm/μM (experimental limit of detection (LOD) = 2.5 nM) for L-Trp and 11.579 nm/μM (experimental LOD = 5.2 nM) for D-Trp. The fiber Bragg grating (FBG) is used as a temperature sensor to compensate the temperature of the sensing system. Chiral detection of tryptophan enantiomers at different temperatures can be achieved by constructing a three parameters matrix. Research has shown that the sensor has a satisfactory application prospect.
研究领域:
生物化学
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光学传感
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材料科学
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Hanyu Wang,Yukun Ouyang,Wenjing Zou,Xingchong Liu,Haimin Li,Ruonan Zhou,Xian Peng,Xiaoli Gong
Perovskite defect passivation with molecule doping shows great potential in boosting the efficiency and stability of perovskite solar cells (PSCs). Herein, an efficient and low-cost bifunctional Lewis base additive d-tryptophan is introduced to control the crystallization and growth of perovskite grains and passivation defects. It is found that the additive doped in the solution precursors could retard crystal growth by increasing activation energy, resulting in improved crystallization of large grains with reduced grain boundaries, as well as inhibiting ion migration and PbI2 aggregation. As a result, the PSCs incorporated with d-tryptophan additives achieve an improved power conversion efficiency from 18.18 to 21.55%. Moreover, the d-tryptophan passivation agent improves the device stability, which retains 86.85% of its initial efficiency under ambient conditions at room temperature after 500 h. This work provides Lewis base small-molecule d-tryptophan for efficient defect passivation of the grain boundaries toward efficient and stable PSCs.
研究领域:
材料科学
·
能源技术
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Peng Lei,Ying Zhou,Guomei Zhang,Yan Zhang,Caihong Zhang,Shasha Hong,Yajuan Yang,Chuan Dong,Shaomin Shuang
Construction of convenient and effective method for enantiomer identification is of vital significance for biochemistry and medical science. Herein, we design an effective sensor for chiral recognition of tryptophan (Trp) enantiomers, and self-assembly of Cu 2+ -modified β-cyclodextrin on poly- L -arginine/multi-walled carbon nanotubes (Cu-β-CD/PLA/MWCNTs) is studied. Meanwhile, Cu 2+ acts as a cap to prevent the release of the high energy water and compel Trp enantiomer into the smaller opening of β-cyclodextrin. Recognition of L -Trp is accomplished by the formation of hydrogen bonds between the amino of L -Trp and the high energy water confined in cavity of Cu-β-CD. Compared with D -Trp, the sensor exhibits favorable chiral recognition toward L -Trp with a separation coefficient of 3.37. And the chiral sensor presents admirable enantiomers determination with excellent sensitivity, providing a good linear correlation in the range of 1 × 10 −6 M~5.5 × 10 −5 M, and the detection limit can reach 3.3 × 10 −7 M (S/N = 3). Besides, the proposed sensor has been able to predict the percentage of D -Trp in the racemic mixture, suggesting its potential applications in the enantiomer recognition field.
研究领域:
生物化学
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医学科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Yanan Deng,Zihao Zhang,Yuanhao Pang,Xueying Zhou,Yu Wang,Yun Zhang,Yali Yuan
The poly-L-cysteine modified Au nanoparticles (Au@p-L-Cys) were constructed on electrode surface as a highly efficient chiral interface for tryptophan (Trp) enantiomers recognition via one step electropolymerization. With the aid of Cu 2+ , L-Cys residues and D-Trp target formed a sandwich complex D-Trp-Cu 2+ -L-Cys, while L-Trp was unable to form such complex due to the steric hindrance provided by the chiral interface, which was confirmed by the electrochemical and SEM results. With the introduction of ferricyanide probe, D-Trp produced significant current decrease while L-Trp produced a slight current increase, which implied the successful enantioselective recognition of Trp enantiomers (specifically D-Trp) in the true sense. This novel sensor showed a surprisingly wide linear range toward D-Trp of 6 × 10 −7 M to 1 × 10 −2 M, with a detection limit as low as 75 nM (S/N = 3). Moreover, the exclusive enantioselectivity toward D-Trp was discovered since other amino acids showed negligible interference to detection of D-Trp. The recovery of D-Trp in human serum was between 91.30 and 109.3%, which further verified the satisfying specificity and practicality of the proposed strategy. The coordination thermodynamics by UV–Vis spectroscopy and DFT simulation were also used to investigate the enantioselective mechanism. These results highlight the great potential of using Au@p-L-Cys to construct chiral interface for enantiomers recognition and hold the promise of practical application of electrochemical chiral sensors in fields like pharmaceutics and bioanalysis .
研究领域:
生物化学
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分析化学
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医药科学
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DOI
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 111
·109992-110000
·
IF 3.289
Zhiyong Zhou,Ke Cui,Yu Mao,Wenshuai Chai,Nian Wang,Zhongqi Ren
Enantioseparation of chiral compounds is important for pharmaceutical production. Chiral resolution of racemic tryptophan has attracted increasing attention. Traditional separation methods have the disadvantages of being high cost, energy intensive and environmentally unfriendly. Membrane permeation seems promising for application in the enantioseparation of racemic tryptophan with the advantages of energy efficiency and no additives. However, lots of organic compounds are commonly used for the preparation of various membranes for enantioseparation of racemic tryptophan. Therefore, we prepared a molecularly imprinted self-supported membrane (MISM) using a green and clean method with natural polymer sodium alginate (SA) as the functional polymer, water as the solvent and CaCl2 as the crosslinking agent. The crosslinking by CaCl2 was confirmed by attenuated total reflection Fourier transform infrared (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS) analyses. The surface and internal structures of the MISM and non-imprinted self-supported membrane (NISM) were observed by atomic force microscope (AFM) and scanning electron microscope (SEM). The addition of a template molecule can significantly improve the MISM surface roughness. The compact structures of MISM and NISM were confirmed by the cross-sectional images. The thermo stability of MISM was studied. In addition, the effects of pH and crosslinking time on the swelling behavior of MISM were investigated. A high concentration of OH− in alkali solution can remarkably weaken the electrostatic interaction between Ca2+ and COO−. The effects of preparation conditions, permeation conditions and concentration polarization on the pressure-driven permeation performance of MISM were investigated. Ultrahigh enantioseparation performance in 99% ee of the permeation solution can be obtained under mild conditions. Reducing the concentration polarization by increasing the environmental temperature and continuous stirring is beneficial to obtaining ultrahigh ee. Comparison with previous studies on pressure-driven permeation performance indicates that the proposed green and clean preparation method for a molecularly imprinted self-supported membrane is not only environmentally friendly but also efficient in obtaining ultrahigh ee.
研究领域:
生物化学
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环境科学
·
制药工程
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产品关联
DOI
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2015
·
IF 2.219
Fang Wang,Wencheng Gong,Lili Wang,Zilin Chen
Selective recognition of d -tryptophan ( d -Trp) in the presence of Cu(II) was investigated at poly- l -lysine (p-l-Lys) film using electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). p-l-Lys film was immobilized on a glassy carbon electrode (GCE) by cyclic voltammetry between 0.0 and 1.9 V in 20 mM phosphate buffer solution (pH 8.6). After the p-l-Lys/GCE electrode was incubated with d -Trp solution containing Cu(II) ions, obvious enhancement of electron transfer resistance and decrease of voltammetric current could be observed. If d -Trp was replaced by l -tryptophan (l-Trp), there was no apparent resistance and current changes. Moreover, no resistance and current changes could be found in the absence of Cu(II). It may be due to the formation of Cu complex with l -lysine and d -tryptophan. Finally, this method was successfully applied to monitoring enantiomeric composition of the d -Trp and l-Trp mixtures.
研究领域:
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL THERMODYNAMICS
·
2018
·
IF 2.631
Xiao Feng,Ali Farajtabar,Hu Lin,Gaoquan Chen,Zhiying Wang,Xinbao Li,Hongkun Zhao
The D-tryptophan solubility in aqueous solutions of methanol, ethanol, isopropanol and N , N -dimethylformamide (DMF) was measured by the saturation shake-flask method at temperatures ranging from 283.15 K to 333.15 K. The maximum solubility was found in water for alcohol (1) + water (2) mixtures, and in neat DMF for DMF (1) + water (2) mixture. When the same temperature and mass fraction of the water is given, the highest mole fraction solubility of D-tryptophan was found in (DMF + water). The solid phases were tested by X-ray powder diffraction (XPRD) showing no polymorphic transformation, solvate formation or crystal transition. Three solubility models, i.e. the Jouyban-Acree, van’t Hoff-Jouyban-Acree and Apelblat-Jouyban-Acree were employed to correlate the experimental solubility values so as to obtain thermodynamic parameters. The RAD and RMSD data were no more than 1.65% and 9.40 × 10 −5 , respectively. Linear solvation energy relationship analysis based on Kamlet-Taft parameters indicated that the variation of D-tryptophan solubility depended on π ∗ for methanol (ethanol, isopropanol) (1) + water (2), and cavity term for DMF (1) + water (2). The preferential solvation parameters ( δx 1,3 ) for methanol, ethanol, isopropanol and DMF derived by means of the inverse Kirkwood–Buff integrals were negative in the four solvent mixtures with intermediate and alcohol-rich compositions, which indicated that D-tryptophan was preferentially solvated by water. The higher solvation by water could be explained in terms of the higher acidic behaviour of the solvents interacting with the Lewis basic groups of the D-tryptophan. However D-tryptophan is preferentially solvated neither by water nor by DMF in DMF + water mixture.
研究领域:
化学
·
生物学
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 4
·1-9
·
IF 5.705
Song Jinyi,Yang Chengcheng,Ma Jiao,Han Qian,Ran Peiyao,Fu Yingzi
An electrochemical chiral multilayer nanocomposite was prepared by modifying a glassy carbon electrode (GCE) via opposite-charge adsorption of amino-modified β-cyclodextrin (NH 2 -β-CD), gold-platinum core-shell microspheres (Au@Pts), polyethyleneimine (PEI), and multi-walled carbon nanotubes (MWCNTs). The modified GCE was applied to the enantioselective voltammetric determination of tryprophan (Trp). The Au@Pts enable an effective immobilization of the chiral selector (NH 2 -β-CD) and enhance the electrochemical performance. Scanning electron microscopy, transmission electron microscopy, UV-vis spectroscopy, FTIR and electrochemical methods were used to characterize the nanocomposite. Trp enantiomers were then determined by differential pulse voltammetry (DPV) (with a peak potential of +0.7 V vs. Ag/AgCl). The recognition efficiency was expressed by an increase in peak height by about 32% for DPV determinations of L-Trp compared to D-Trp in case of a 5 mM Trp solution of pH 7.0. Response was linear in the 10 μM to 5.0 mM concentration range, and the limits of detection were 4.3 μM and 5.6 μM with electrochemical sensitivity of 43.5 μA·μM −1 ·cm −2 and 34.6 μA·μM −1 ·cm −2 for L-Trp and D-Trp, respectively (at S/ N = 3).
Schematic of an electrochemical chiral multilayer nanocomposite composed of multi-walled carbon nanotubes (MWCNTs), polyethyleneimine (PEI), gold-platinum core-shell microspheres (Au@Pt) and amino-modified β-cyclodextrin (NH 2 -β-CD). It was prepared by modifying a glassy carbon electrode (GCE) for enantioselective voltammetric determination of tryptophan (Trp) enantiomers.
研究领域:
分析化学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Physical Chemistry C
·
2015
·
IF 4.772
Yongxin Tao,Xiaogang Gu,Linhong Deng,Yong Qin,Huaiguo Xue,Yong Kong
We report a Cu2+-modified β-cyclodextrin, Cu2-β-CD, that was self-assembled on poly(l-glutamic acid) (P-l-Glu) for chiral recognition of d-tryptophan (d-Trp). Cu2+ formed binuclear hydroxy-bridge (Cu(OH)2Cu) at the wider opening of the cavity of β-CD and acted as a cap to prevent high-energy water molecules from being released while forcing Trp isomers to enter through its narrower opening. Because H-bonds were favored to form between the high-energy water molecules and the amino groups of d-Trp inside the cavity of β-CD, d-Trp was thus recognized. The recognition capability of the Cu2-β-CD appeared to depend on the way the Cu2-β-CD self-assembled onto P-l-Glu, preferring via H-bonds rather than via electrostatic interactions, because electrostatic interactions between P-l-Glu and Cu(OH)2Cu could alter the configuration of the Cu2-β-CD cavity, resulting in weakened H-bonds between high-energy water molecules and d-Trp.
研究领域:
生物化学
·
材料科学
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产品关联
DOI
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2023
·Vol. 39
·期 6
·976-984
·
IF 3.1
Hou Huipeng,Tang Shanshan,Wang Wei,Liu Miao,Liang Axin,Xie Bingteng,Yi Yue,Luo Aiqin
I n this work, an electrochemical chiral sensor of a nanowire-like chiral metal-organic framework/multiwalled carbon nanotube-chitosan/glassy carbon electrode( D -MOF/MWCNTs-CS/GCE) was proposed for the enantiorecognition of L / D -tryptophan( L / D -Trp). The asymmetrical spatial structure of D -MOF provides the feasibility for the enantiorecognition of Trp enantiomers. Moreover, differential pulse voltammetry(DPV) was carried out to be the detection method and the DPV peak potential difference(Δ E p ) between L -Trp and D -Trp was referred as the index of the enantiorecognition performance. Several parameters, such as mass ratios and drop-coated volume of MWCNTs-CS, drop-coated volume and concentration of D -MOF, pH and detection temperature of D -MOF/MWCNTs-CS/GCE were optimized for the largest Δ E p value. And the molecular dynamics(MD) simulation was used to elucidate the enantiorecognition mechanism. Furthermore, the proportions of D -Trp( D -Trp%) in Trp mixtures were detected in a good linear relationship with the DPV peak potentials( E p ), and the proposed electrochemical chiral sensor exhibited good reproducibility, stability and enantiorecognition ability. Additionally, the proposed electrochemical chiral sensor( D -MOF/MWCNTs-CS/GCE) has a good application prospect in the fields of biomedicine, clinical diagnosis, chemical production, pharmaceuticals safety and so on.
研究领域:
分析化学
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生物医学
·
电化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）