ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2022
·
IF 7.129
Di Ju,Youssef Dewer,Shipan Zhang,Chao Hu,Peirong Li,Xueqing Yang
The codling moth Cydia pomonella L. (Lepidoptera: Tortricidae) is one of the most notorious pests of pome fruits and walnuts worldwide, which has developed resistance to almost all classes of insecticides, including abamectin (ABM). ATP-binding cassette (ABC) transporters are thought to play a vital roles in insecticide detoxification by reducing the toxic concentrations of insecticides in an organism tissues. Despite the tremendous progress in understanding the detoxification mechanisms at the molecular level, the physiological functions of ABC transporters in insects have been poorly investigated. In this study, we found that the ABC inhibitor verapamil synergized significantly the toxicity of ABM, suggesting a potential role of ABC in detoxification. A total of 54 ABC genes were identified in the third-instar larvae of C. pomonella after treatment with sublethal doses (LD 10 and LD 30 ) of ABM. The expression profile of these genes in ABM-treated larvae at different time points (24, 48, 72 hr) using transcriptomic analysis (RNA-seq) was also investigated. The results showed that the expression of about 30 ABC genes was significantly co-upregulated after treatment. Several specific genes were up-regulated at 48 hr after treatment of larvae with LD 10 ABM. Among these up-regulated genes, we found that the relative expression level of the CPOM19553 was 29.7-fold and 16.0-fold higher when larvae were exposed to ABM at the LD 10 and LD 30 doses compared to control, respectively. Unlike other ABC genes, only CPOM08323 exhibited significant expression levels in the head and cuticle of the third-instar larvae of C. pomonella exposed to the two sublethal doses of ABM, with no expression was observed in the detoxification tissues such as midgut and Malpighian tubule. This study suggests that these up-regulated genes may be involved in ABM resistance in C. pomonella . Our findings will provide an additional information required for further analysis of ABC transporter genes associated with xenobiotic metabolism in C. pomonella .
研究领域:
昆虫学
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分子生物学
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毒理学
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DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
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2022
·
IF 7.968
Junlin Xie,Kun Wei,Xiaoqing Liu,De Fang
Carbon powders were used to detoxify [Cr(VI)] in metallurgical solid waste was investigated, since the presence of [Cr(VI)] had a very serious impact on the environment. Five types of carbon powders were selected as carbon sources and soluble CrO 3 was used as a hexavalent chromium source in metallurgical solid waste. Ultra-fine Cr 3 C 2 powders (about 80 nm) were synthesized by carbothermal reduction reaction to achieve the detoxification of [Cr(VI)]. The temperature plays a crucial role in the synthesis of Cr 3 C 2 according the experimental and thermodynamic results, illustrating that the synthesis temperature was in the range of 900 ℃–1200 ℃. Surface area, morphology, and surface chemistry of carbon powders were investigated to analyze the effect of carbon type on the mechanism of detoxification of hexavalent chromium [Cr(VI)] and the formation of chromium carbide . The results show that nano carbon powder with finer particle size (30–50 nm), higher specific surface area (101.5 m 2 /g) and fewer chemical bonds with carbon atoms formed by sp 2 orbital hybridization are more suitable as a carbon source for carbothermal reduction of [Cr(VI)]. Therefore, nano Cr 3 C 2 powder was achieved at the lowest synthesis temperature (900 ℃), when nano carbon powder was selected as the carbon source.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2022
·
IF 6.056
Le Wang,Pengxue Sun,Mingna Li,Yan Shen,Yangyong Lv,Hailong Tian,Dapeng Wu,Ying Liang,Qipeng Yuan,Jinshui Wang
A novel biological detoxification polymer (BDP) was in-situ synthesis by the co-immobilization of the compound enzymes (laccase and Aspergillus niger crude enzyme) and the aminated mesoporous silica nanoparticles (MSN-NH 2 ) with zeolitic imidazolate framework-8 (ZIF-8) for the detoxification of aflatoxin B 1 (AFB 1 ). The ZIF-8 shell of BDP acted as the AFB 1 adsorbent around the compound enzymes inside the BDP. The detoxification rate of BDP at 35 °C for 24 h was 96.7%. The detoxification of AFB 1 with the added mass ratio of BDP 1% to moldy corn was 65.8%. After the BDP was stored at 4 °C for 30 d, the enzyme activity remained 70.9%. Moreover, the enzyme activity and detoxification rate with BDP remained 80.7% and 80.0%, respectively, after the fourth repeated detoxification. The compound enzymes acted on the C–O bond and methoxyl group in the side chain of AFB 1 . Three degradation products (C 16 H 16 O 5 , C 16 H 14 O 4 , C 15 H 14 O 2 ) with different structures were detected in the degradation of AFB 1 with the BDP. Application of the in-situ BDP represents a promising way to improve the detoxification of AFB 1 .
研究领域:
食品科学
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生物技术
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环境科学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yunhai Zhang,Zihao Chen,Xuebing Cao,Zhen Xu,Anlin Xu,Yide He,Qiqi Zhang,Sven Uwe Geissen,Yongjun Zhang
Ozonation, a widely investigated and applied technology to eliminate organic contaminants from water, is confronted with the unintended generation of toxic transformation products (TPs). In this study, we investigated the confinement effect on the detoxification of ozonation for the first time via comparing the confined catalyst and its non-confined peer, i.e. FeMn nanoparticles in mesopores of zeolite SBA-15 and FeMn on the exterior of zeolite. Material characterization and catalytic kinetics demonstrated that both catalysts shared an identical instinct catalytic behavior, but reactive oxygen species contributed a higher ratio to the oxidation of organics in the confined system than the non-confined peer. It was found that the confined system led to a significantly low toxicity of enrofloxacin TPs measured with the respiratory inhibition of bacteria, the development of zebrafish embryo, and the in silico toxicity endpoints, likely due to the distinct compositions and levels of enrofloxacin TPs discovered with mass spectrometry. Degradation tests further revealed that the confined ozonation effectively reduced the interference of organic matrix. The calculation with molecular dynamics demonstrated that the diffusion retarding effects of mesopores can leverage the oxidation toward small target contaminants over large matrix organics. The superior detoxification and anti-interference capacity of confined ozonation, demonstrated in this study, inspires a promising pathway to tackle the application challenges of catalytic oxidation.
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产品关联
DOI
高影响因子
BRITISH JOURNAL OF PHARMACOLOGY
·
2019
·Vol. 176
·期 24
·4639-4652
·
IF 6.583
Mengjing Zhao,Huan Zhao,Jiangming Deng,Lianxia Guo,Baojian Wu
Background and Purpose Whether and how circadian clock proteins regulate drug detoxification are not known. Here, we have assessed the effects of CLOCK (a core circadian clock protein) on drug metabolism and detoxification. Experimental Approach Regulation by CLOCK protein of drug-metabolizing enzymes was assessed using Clock knockout ( Clock −/− ) mice and Hepa-1c1c7/AML-12 cells. The relative mRNA and protein levels were determined by qPCR and Western blotting respectively. Toxicity and pharmacokinetic experiments were performed with Clock −/− and wild-type mice after intraperitoneal injection of coumarin or cyclophosphamide. Transcriptional gene regulation was investigated using luciferase reporter, mobility shift, and chromatin immunoprecipitation (ChIP) assays. Key Results Clock deletion disrupted hepatic diurnal expressions of a number of drug-metabolizing enzymes in mice. In particular, CYP2A4/5 expressions were markedly down-regulated, whereas CYP2B10 was up-regulated. Positive regulation of Cyp2a4/5 and negative regulation of Cyp2b10 by CLOCK were confirmed in Hepa-1c1c7 and AML-12 cells. Based on a combination of luciferase reporter, mobility shift, and ChIP assays, we found that CLOCK activated Cyp2a4/5 transcription via specific binding to E-box elements in promoter region and repressed Cyp2b10 transcription through REV-ERBα/β (two target genes of CLOCK and transcriptional repressors of Cyp2b10 ). Furthermore, Clock ablation sensitized mice to coumarin toxicity by down-regulating CYP2A4/5-mediated metabolism (a detoxification pathway) and to cyclophosphamide toxicity by up-regulating CYP2B10-mediated metabolism (generating the toxic metabolite 4-hydroxycyclophosphamide). Conclusion and Implications CLOCK protein regulates metabolism by the cytochrome P450 family and drug detoxification. The findings improve our understanding of the crosstalk between circadian clock and drug detoxification.
研究领域:
药理学
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分子生物学
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昼夜节律
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产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2025
·
IF 4
Bing Bai,Huan-Juan Zhao,Bo-Kun Wang,Yu-Ting Li,Ping Gao,Yun-Tong Lv,Xue-Qing Yang
During long-term plant-insect interactions, plants have evolved diverse plant secondary metabolites (PSMs) as defenses against herbivory, while insects have developed sophisticated detoxification mechanisms to neutralize these compounds. Invasive and native herbivorous species may employ distinct metabolic strategies to cope with PSMs, a phenomenon that remains incompletely characterized. Here, we report the differential detoxification strategies of two closely related fruit borers, the invasive Cydia pomonella and the native Grapholita molesta , which exhibit interspecific competition, in response to quercetin stress—a flavonoid present in their host fruits. C. pomonella requires more generations to adapt 70 μg/g quercetin, indicating differential tolerance to flavonoid toxicity. Metabolic profiling shows G. molesta primarily detoxifies quercetin mainly via synergistic cytochrome P450 (P450) and transmembrane transport, whereas C. pomonella shifts from transport to a predominant role of oxidation over time. Given that C. pomonella was not pre-adapted to high quercetin stress in its native habitat, its constitutive defense system was likely suppressed under quercetin exposure. This species compensated by upregulating ATP-binding cassette (ABC) transporter-mediated excretion, as evidenced by HPLC quantification which indicated that the fecal quercetin content exceeded 50 % of the administered dose. Correlation analysis suggests that C. pomonella employs a biphasic detoxification: initial short-term dominated by ABC transporter activity, followed by a long-term reliance on P450-mediated metabolism. Conversely, G.molesta primarily utilizes a synergistic enzymatic detoxification system involving multiple enzymes. These findings elucidate divergent adaptive mechanisms to quercetin stress in the two fruit borers and enhance understanding of herbivorous insect-plant co-evolution and the interspecific interactions between invasive and native pest species.
研究领域:
昆虫学
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植物-昆虫互作
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生态适应
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生化毒理学
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入侵物种生物学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2022
·
IF 9.988
Zhaochao Deng,Ning Zhang,Lijia Jiang,Hui Liu,Songtao Hu,Dongdong Zhang,Bairu Chen,Qing Liu,Yuxia Sun,Jiawang Chen,Chunfang Zhang
Microplastics (MPs) can absorb halogenated organic compounds and transport them into marine anaerobic zones. Microbial reductive dehalogenation is a major process that naturally attenuates organohalide pollutants in anaerobic environments. Here, we aimed to determine the mechanisms through which MPs affect the microbe-mediated marine halogen cycle by incubating 2,4,6-trichlorophenol (TCP) dechlorinating cultures with various types of MPs. We found that TCP was dechlorinated to 4-chlorophenol in biotic control and polypropylene (PP) cultures, but essentially terminated at 2,4-dichlorophenol in polyethylene (PE) and polyethylene terephthalate (PET) cultures after incubation for 20 days. Oxygen-containing functional groups such as peroxide and aldehyde were enriched on PE and PET after incubation and corresponded to elevated levels of intracellular reactive oxygen species (ROS) in the microorganisms . Adding PE or PET to the cultures exerted limited effects on hydrogenase and ATPase activities, but delayed the expression of the gene encoding reductive dehalogenase (RDase). Considering the limited changes in the microbial composition of the enriched cultures, these findings suggested that microbial dechlorination is probably affected by MPs through the ROS-induced inhibition of RDase synthesis and/or activity. Overall, our findings showed that extensive MP pollution is unfavorable to environmental xenobiotic detoxification.
研究领域:
环境科学
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微生物学
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污染研究
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产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2025
·
IF 3.8
Linlin Shi,Lei Yang,Zixuan Yan,Chang Xu,Wenhui Li,Tingting Wang,Quan Gao,Min Liao,Haiqun Cao,Qingkui Fang
Background Prothioconazole (PTC), a triazole fungicide, and its metabolite prothioconazole-desthio (dPTC) present potential phytotoxic risks in crops. However, the mechanisms governing their uptake and detoxification in wheat remain unknown. This study aimed to determine how macronutrients and endogenous signaling compounds regulate PTC absorption and metabolism in wheat seedlings. Results High potassium availability markedly enhanced PTC uptake, resulting in a > 600% increase in PTC uptake over 12 h compared to the low potassium. Exogenous application of indole-3-acetic acid and brassinolide led to significant increases in PTC accumulation by 121.7% and 94.4%, respectively. Transcriptomic analysis revealed that PTC activated detoxification pathways, with prominent up-regulation of adenosine triphosphate (ATP)-binding cassette transporters and mitogen-activated protein kinase (MAPK) signaling components. Under nitrogen-deficient conditions, 1493 genes were specifically down-regulated, including those related to ribosome biogenesis, phenylalanine metabolism, and membrane transport, indicating a reduced detoxification capacity and enhanced dPTC retention. Conclusion Nitrogen availability plays a pivotal role in modulating the molecular response of wheat to PTC exposure. Nitrogen deficiency markedly down-regulated key genes involved in detoxification processes, thereby aggravating PTC-induced phytotoxicity. These results underscore the critical interplay between nutrient status and pesticide metabolism, providing mechanistic evidence to inform integrated pesticide–fertilizer management strategies aimed at minimizing chemical residues in food crops and enhancing agroecosystem sustainability. © 2025 Society of Chemical Industry.
研究领域:
植物生理学
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农药代谢
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作物营养
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分子生物学
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环境毒理学
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农业可持续性
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产品关联
DOI
PubMed
高影响因子
Materials Advances
·
2022
·Vol. 3
·期 8
·3593-3599
·
IF 0
Shanshan Xing,Chunqiu Xia,Xinyi Liu,Liangqia Guo,Fengfu Fu
A simple and green one-step method for the simultaneous exfoliation and functionalization of few-layered MoS2 nanosheets was developed via the ultrasonic treatment of bulk MoS2 powder in sodium phytate-containing aqueous solution. The as-prepared MoS2 nanosheets show high stability, low cytotoxicity and high adsorption capacity for Hg2+ in aqueous solution. The maximum adsorption capacity for Hg2+ is about 7.5 times and 3.7 times that of bulk MoS2 powder and unfunctionalized MoS2 nanosheets, respectively. The adsorption kinetics and thermodynamics indicate that the adsorption of Hg2+ is a monolayer chemical adsorption. Finally, the as-prepared MoS2 nanosheets were applied for the removal of Hg2+ in HepG2 cells and show a similar detoxification effect as that of the common antidote meso-2,3-dimercaptosuccinic acid, indicating the potential application of MoS2 nanosheets for biological detoxification.
研究领域:
材料科学
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环境科学
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生物医学工程
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纳米技术
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产品关联
DOI
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2018
·Vol. 25
·期 33
·33402-33414
·
IF 2.8
Wang Chongnv,Bourland William A.,Mu Weijie,Pan Xuming
Chlorpyrifos (CPF) pollution has drawn widespread concerns in aquatic environments due to its risks to ecologic system, however, the response mechanisms of ciliates to CPF pollution were poorly studied. In our current work, the degradation of CPF by ciliates and the morphological changes of ciliates after CPF exposure were investigated. In addition, the transcriptomic profiles of the ciliate Uronema marinum , with and without exposure with CPF, were detected using digital gene expression technologies. De novo transcriptome assembly 166,829,634 reads produced from three groups (untreated, CPF treatment at 12 h and 24 h) by whole transcriptome sequencing (RNA-Seq). Gene ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathways were analyzed in all unigenes and different expression genes to identify their biological functions and processes. Furthermore, the results indicated that genes related to the stress response, cytoskeleton and cell structure proteins, and antioxidant systems might play an important role in the resistance mechanism of ciliates. The enzyme activities of SOD and GST after CPF stress were also analyzed, and the result showed the good antioxidant capacity of SOD and GST in ciliates inferred from the increase of the activities of the two enzymes. The ciliate Uronema marinum showed a resistance response to chlorpyrifos stress at the transcriptomic level in the present work, which indicates that ciliates can be considered as a potential bioremediation agent.
研究领域:
环境科学
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分子生物学
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生态毒理学
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基因组学
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产品关联
DOI
PubMed
高影响因子
Electrocatalysis
·
2025
·
IF 2.8
Yang Zehong,Wang Zihao,Qiu Qiuhong,Luo Yuzhan,Wang Chenglong,Huang Yaobin,Zhuang Tao,Zheng Weijin,Chen Liang,Lan Liying,Liu Guangli,Fang Jiasheng,Li Yanliang,Lv Sihao,Wang Wenlong,Qiu Yongfu,Zhuo Qiongfang
A Sn/ZnO 2 @Ti 4 O 7 anode was prepared using a combination of hydrothermal synthesis and discharge plasma sintering methods for the electrochemical degradation of perfluorooctanoic acid (PFOA). The introduction of Sn/ZnO 2 significantly increased the electrochemical active surface area (ECSA) and the oxygen evolution potential (OEP) of Ti 4 O 7 . A 97.6% degradation rate of PFOA can be achieved using a Sn/ZnO 2 @Ti 4 O 7 anode, at a current density of 40 mA/cm 2 , with the initial PFOA concentration of 10 mg/L, at a pH value of 5.69, and within 120 min. Radical quenching experiments indicated that direct oxidation was the main mechanism for PFOA degradation, contributing 82.5%. The degradation efficiencies of PFOA remained above 88% in Dongjiang and Zhujiang rivers (DJR and ZJR) matrixes, confirming the system’s resistance to complex matrix. The total organic carbon (TOC) removal ratio was 56.8%, and the defluorination ratio was 48% after electrolysis of 6 h. After 36 cycles of degradation, the degradation rate was 95.6%. The toxicity assessments indicated that the degradation intermediates were significantly less toxic than the parent PFOA. Graphical
研究领域:
环境科学
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电化学
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水处理
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污染物降解
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材料科学
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产品关联
DOI
高影响因子
CHEMOSPHERE
·
2021
·
IF 7.086
Yimin He,Weichuan Lin,Ce Shi,Ronghua Li,Changkao Mu,Chunlin Wang,Yangfang Ye
Dibutyl phthalate (DBP) is one of the most commonly used and toxic phthalate esters and has a variety of harmful effects on aquatic animals. However, there is still a lack of knowledge on the accumulation, detoxification, and toxicity of DBP in aquatic animals. In this study, we chose the swimming crab Portunus trituberculatus , an ecologically and economically important species, as the model and investigated the metabolism of DBP and its effects on the detoxification, antioxidation, survival and growth of the crab juveniles to better understand DBP-triggered molecular response over different time courses. As a result, DBP could be accumulated in the swimming crab in a concentration-dependent manner and metabolized to monobutyl phthalate (MBP) and phthalic acid (PA) through de-esterification. DBP exposure induced the different responses of three cytochrome P450 members and antioxidant enzyme genes, enhanced gene transcript and protein levels of glutathione-S-transferase and two heat stress proteins and malondialdehyde accumulation, decreased glutathione level, and inhibited antioxidant enzyme activities . Further, no significant effect of DBP was observed in crab survival, size, and weight but there was molting retardation. Therefore, DBP induced strong detoxification and antioxidative defense mechanisms to overcome detrimental effects of DBP on the swimming crab juveniles despite a molting retardation as a trade-off in fitness costs. The prevalent coexistence of DBP with MBP and PA during the whole exposure period is raising concerns on the combined action and ecological risk to aquatic animals.
研究领域:
环境科学
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水生毒理学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）