CHEMOSPHERE
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2023
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IF 8.8
Lu Qiao,Lu Chen,Zhen Yang,Jinhua Xu,Jinlong Song,Xingyang Li,Huiwu Sun,Yingchun Mu
Diflubenzuron has been applied in agriculture and aquaculture, and its residues in ecological environment and food chain could result in chronic exposure and long-term toxicity effects for human health. However, limited information is available regarding diflubenzuron levels in fish and associated risk assessment. This study performed the analysis for dynamic bioaccumulation and elimination distribution of diflubenzuron in carp tissues. The results indicated that diflubenzuron was absorbed and enriched by fish body along with higher enrichment in lipid-rich tissues of fish. The peak concentration in carp muscle reached 6-fold of diflubenzuron concentration in aquaculture water. The median lethal concentration (LC 50 ) of diflubenzuron at 96 h was 12.29 mg/L, presented low toxicity to carp. Risk assessment results showed that the chronic risk from dietary exposure to diflubenzuron through carp consumption for Chinese residents of children and adolescents, adults and elderly people were acceptable, while posed a certain risk for young children . This study provided the reference for pollution control , risk assessment and scientific management of diflubenzuron.
研究领域:
环境科学
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毒理学
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水产养殖学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Xian-Xian Ji,Yu-Long Liu,Xin-Yue Chang,Rui-Long Li,Fei Ye,Liu Yang,Ying Fu
Diflubenzuron is widely used as a benzoylurea insecticide, and its impact on human health should not be underestimated. Therefore, the detection of its residues in food and the environment is crucial. In this paper, octahedral Cu-BTB was fabricated using a simple hydrothermal method. It served as a precursor for synthesizing Cu/Cu 2 O/CuO@C with a core–shell structure through annealing, creating an electrochemical sensor for the detection of diflubenzuron. The response of Cu/Cu 2 O/CuO@C/GCE , expressed as Δ I / I 0 exhibited a linear correlation with the logarithm of the diflubenzuron concentration ranging from 1.0 × 10 −4 to 1.0 × 10 −12 mol·L −1 . The limit of detection (LOD) was determined to be 130 fM using differential pulse voltammetry (DPV). The electrochemical sensor demonstrated excellent stability, reproducibility, and anti-interference properties. Moreover, Cu/Cu 2 O/CuO@C/GCE was successfully employed to quantitatively determine diflubenzuron in actual food samples (tomato and cucumber) and environmental samples (Songhua River water, tap water, and local soil) with good recoveries. Finally, the possible mechanism of Cu/Cu 2 O/CuO@C/GCE for monitoring diflubenzuron was thoroughly investigated.
研究领域:
分析化学
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环境科学
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食品科学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2022
·Vol. 23
·期 19
·11932
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IF 6.208
Xue Han,Xiaowen Xu,Tingting Yu,Meifeng Li,Yulong Liu,Jingli Lai,Huiling Mao,Chengyu Hu,Shanghong Wang
Diflubenzuron is an insecticide that serves as a chitin inhibitor to restrict the growth of many harmful larvae, including mosquito larvae, cotton bollworm and flies. The residue of diflubenzuron is often detected in aquaculture, but its potential toxicity to aquatic organisms is still obscure. In this study, zebrafish embryos (from 6 h to 96 h post-fertilization, hpf) were exposed to different concentrations of diflubenzuron (0, 0.5, 1.5, 2.5, 3.5 and 4.5 mg/L), and the morphologic changes, mortality rate, hatchability rate and average heart rate were calculated. Diflubenzuron exposure increased the distance between the venous sinus and bulbar artery (SV-BA), inhibited proliferation of myocardial cells and damaged vascular development. In addition, diflubenzuron exposure also induced contents of reactive oxygen species (ROS) and malondialdehyde (MDA) and inhibited the activity of antioxidants, including SOD (superoxide dismutase) and CAT (catalase). Moreover, acridine orange (AO) staining showed that diflubenzuron exposure increased the apoptotic cells in the heart. Q-PCR also indicated that diflubenzuron exposure promoted the expression of apoptosis-related genes (bax, bcl2, p53, caspase3 and caspase9). However, the expression of some heart-related genes were inhibited. The oxidative stress-induced apoptosis damaged the cardiac development of zebrafish embryos. Therefore, diflubenzuron exposure induced severe cardiotoxicity in zebrafish embryos. The results contribute to a more comprehensive understanding of the safety use of diflubenzuron.Keywords:diflubenzuron;zebrafish embryo;cardiotoxicity;oxidative stress;apoptosis
研究领域:
毒理学
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环境科学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Polymer-Plastics Technology and Materials
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2020
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IF 0
Kaixuan Nian,Lei Zheng,Xiaomin Cheng
In this work, diflubenzuron (DBZ) molecularly imprinted polymers (MIPs) were synthesized by precipitation polymerization. The polymers were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic light scattering (DLS). The influences of synthetic conditions including template molecules, functional monomers and crosslinker on the surface morphology, particle size and size distribution of polymers were investigated. Scatchard analysis indicated the presence of specified recognition sites in MIPs. The Sips model was fitted better to the equilibrium data of MIPs over whole concentrations. The pseudo-second-order kinetic model was well fitted to the experimental data of adsorption kinetic experiment, suggesting the chemisorption mechanism between MIPs and DBZ. Four structurally similar compounds were used for the selectivity test of MIPs. It was observed that MIPs can selectively rebind the template molecules (DBZ) under the interference of four other structurally similar compounds. The application of MIPs in solid-phase extraction (SPE) offers a method for extraction and determination of benzoylureas (BUs) pesticides in apple samples prior to high-performance liquid chromatography (HPLC) analysis.
研究领域:
分析化学
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食品科学
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环境科学
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DOI
Insects
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2022
·Vol. 13
·期 8
·728
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IF 3.139
Cong Zhang,Wenfeng Hu,Zhuo Yu,Xian Liu,Jing Wang,Tianrong Xin,Zhiwen Zou,Bin Xia
Simple SummaryThe transcriptional level of chitin synthase A (DcCHSA) was investigated inDiaphorina citriKuwayama at various developmental stages and tissues in this work. The survival and molting ofD. citrinymphs were negatively impacted by high concentrations of DFB, which could also causeDcCHSAto express itself significantly. We also looked into how CHS knockout affected nymph development and eclosion. The findings revealed thatDcCHSAwas critical for the eclosion and molting ofD. citrinymphs. This study provides a solid theoretical foundation forD. citrimanagement in China.AbstractDiaphorina citriKuwayama is the vector of HLB and one of the most common pests in citrus orchards in southern China. One of the most significant genes inD. citri’s growth and development is the chitin synthase gene. In this study, the CHS gene (DcCHSA) ofD. citriwas cloned and analyzed by bioinformatics. According to RT-qPCR findings,DcCHSAwas expressed at many growth processes ofD. citri, with the greatest influence in the fifth-instar nymph. The molting failure rate and mortality ofD. citrirose as DFB concentration increased in this research, as did the expression level ofDcCHSA. Feeding onDcCHSAcaused a large drop in target gene expression, affected nymph molting, caused failure or even death in freshly eclosion adults, increased mortality, and reduced the molting success rate over time. These findings showed thatDcCHSAwas involved in nymph to adult development and may aid in the identification of molecular targets forD. citriregulation. It provided new ideas for further control ofD. citri.Keywords:Diaphorina citri;chitin;diflubenzuron;transcription level;RNA interference
研究领域:
昆虫学
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分子生物学
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害虫管理
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Yujiao Wang,Xiaoyan Cui,Runhua Lu,Haixiang Gao,Huafen Li,Wenfeng Zhou
The Tyndall Effect assay (TEA) has been applied into colorimetric metal ion detection since 2019. However, the TEA-based sensor for pesticide detection has never been reported till now. Herein, a facile fluorescent organic nanoparticle (FON)-based sensor is firstly developed for fluorine-containing pesticide detection through ratiometric fluorescence assay (FLA) and TEA. For FLA, the intensity of the second-order Tyndall scattering peak (STS 590nm ) and the fluorescence peak of the FON-based sensor would increase and remain unchanged respectively when adding bifenthrin, flufenoxuron, and diflubenzuron. The detection limits were respectively 9.34, 6.91, and 3.60 μg/kg. For TEA, the increased STS 590nm intensity displayed a bright and visible light beam. An economical, simple, and portable device was then constructed to visually monitor the analytes. The sensor was successfully used to detect the analytes in teas through FLA and TEA with the recoveries and RSD ranging from 86.27–100.00 %, and 0.00–5.68 %, respectively.
研究领域:
分析化学
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环境科学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）