TALANTA
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2025
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IF 6.1
Huifang Wu,Qiqi Wang,Lingfeng Zhu,Haoliang Zhang,Lizhong Liu
Anthrax, a fatal infectious disease caused by Bacillus anthracis ( B. anthracis ), poses a significant threat to public health. Herein, a novel type of carbon dots rich in π → π∗ transitions ( R -CDs) was developed to fabricate an R -CDs/Eu 3+ hybrid for the rapid on-site detection of the B. anthracis biomarker dipicolinic acid (DPA). The R -CDs/Eu 3+ hybrid exhibits two distinct fluorescence responses to DPA: (1) quenching of the R -CDs fluorescence due to the internal filter effect, and (2) enhancement of Eu 3+ fluorescence resulting from the antenna effect and the protective role of R -CDs, enabling ratiometric detection of DPA over a range of 0.1–100 μM with excellent selectivity. Furthermore, a portable, cost-effective device based on the hybrid was developed, enabling in-filed detection of DPA in lake water and real-time monitoring of DPA release from bacterial spores, which holds great promise for monitoring pathogenic biomarkers, with significant implications water safety and human health.
研究领域:
生物化学
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分析化学
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公共卫生
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DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2018
·Vol. 54
·期 3
·2526-2534
·
IF 2.993
Zhou Zhan,Wang Zhuosen,Tang Yiping,Zheng Yuhui,Wang Qianming
Bacillus anthracis marker (dipicolinic acid, DPA) is a biological warfare agent. The excessive amounts of the compound would lead to serious problems in human health. It is essential to develop a straightforward and rapid detection technology of DPA. For this reason, an optical sensing platform was established on a robust alginate film. Firstly, green emission CQDs were synthesized via a one-step microwave carbonization method based on citric acid and urea. In the next step, europium ions were employed to chelate the carboxyl groups of CQDs and sodium alginate was selected to assemble the green fluorescent film (CQDs-Eu film). In the presence of DPA, the emission peaks evolved from green to red region. The ratio of I 615 / I 554 linearly increased with the addition of DPA in the range of 1–45 µM with a detection limit as low as 0.17 µM. This CQDs-Eu film-based sensing nanostructure showed its potential applications in the fields of biology and food analysis.
研究领域:
生物传感
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环境科学
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光学检测
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DOI
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 190
·期 1
·1-11
·
IF 6.408
Xu Yixuan,Shi Xuerong,Ran Fanpeng,Zhang Ziqi,Phipps Josh,Liu Xiaoyan,Zhang Haixia
A novel Tb-doped Eu-based metal–organic framework (Eu-MOF@Tb) has been developed by incorporating hexanuclear europium cluster and 2,2′-bipyridine-5,5′-dicarboxylic acid as well as coordination with Tb(III). Owing to the diverse coordination status of Tb(III) and Eu(III) in MOF, antenna effect emission from Tb(III) can be invoked by dipicolinic acid (DPA), but the luminescence originating from Eu(III) remains unchanged. Taking advantage of this phenomenon, a ratiometric luminescent method for detection of DPA, a biomarker for Bacillus subtilis spores, was developed through differential sensitization toward lanthanide ions. This analysis method allowed for the detection of DPA in the 0.2–10 μM concentration range, with a detection limit of 60 nM. It was further validated by spiked recoveries (89.3–110%) of real-world samples with RSD values in the range 3.9–11%. Alongside this, a paper indicator test was prepared for naked-eye detection of DPA via a dose-sensitive color evolution from red to green under UV light. The effectiveness of the proposed approach was explored in the detection of bacterial spores in real biological and environmental samples and indicated great potential for applications as a real-time monitoring system against the anthrax threat. Graphical Abstract
研究领域:
分析化学
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生物化学
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环境科学
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DOI
PubMed
高影响因子
ACS Applied Electronic Materials
·
2022
·
IF 4.494
Yuqi Huang,Tiange Li,Mengmeng Lei,Xianqing Huang,Tianlin Wang
In the present investigation, we demonstrated a simple approach for the reproducible synthesis of cerium(III)-based blue phosphor, which provides versatile applications in the construction of warm white-light-emitting diodes (WLEDs) and anticounterfeiting patterns. It is found that the mixture of dipicolinic acid (DPA) with Ce3+ in ethanol (DPA-Ce) emits blue fluorescence under the assistance of ultraviolet (UV) induction. The quantum yield of the DPA-Ce phosphor reaches 44% and is improved to 52% by combining with guanosine 5′-monophosphate (GMP). The obtained DPA-Ce-GMP emits blue fluorescence in both a solid powder and aqueous solution, having advantages of long luminescence lifetimes, ultralong stability, and outstanding antitemperature bleaching. With DPA as a universal ligand, DPA-Tb with green fluorescence and DPA-Eu with red fluorescence are integrated with the blue fluorescence of DPA-Ce-GMP to construct full-color and white-light-emitting devices. The as-prepared warm WLED with a quantum yield of 64% presents an excellent stability and high quality with a color rending index of up to 90, CIE color coordinates of (0.37, 0.36), a correlated color temperature of 3950 K, and a luminous efficiency of 37.8 lm W–1. Multiple anticounterfeiting patterns are further encoded with the as-prepared lanthanide-based phosphors. The investigation offers a simple assay for the cost-effective, large-scale synthesis of phosphors, with great promise in diverse applications including the lighting industry and anticounterfeiting technology.
研究领域:
材料科学
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光学工程
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应用化学
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DOI
高影响因子
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
·
2021
·
IF 4.32
Yang Mu,Fuyin Zhang,Ning Li,Shanshan Pi,Ang Li,Qing Hong,Jian He,Jiguo Qiu,Jiandong Jiang
Dipicolinic acid (DPA) is a widely distributed pyridine derivative containing two carboxylate groups. However, the catabolism of DPA mediated by microorganisms are rarely reported. Alcaligenes faecalis strain JQ135, previously isolated in our lab, was able to degrade various pyridine derivatives, including nicotinic acid (NA). In this study, it was found that strain JQ135 could utilize DPA as the sole carbon and nitrogen source for growth and degrade 1.8 mM (300 mg/L) DPA to a non-detectable level within 12 h. The catabolism of DPA by strain JQ135 extracted two intermediates, i.e., 3-hydroxy-2,6-dipicolinic acid and 4-imino-pent-2-enedioic acid. Moreover, deletion of the key genes essential for NA catabolism (such as moaCDEBmoeA and maiA ) did not affect DPA catabolism, indicating that the maleamate pathway and molybdenum cofactor are not involved in the DPA catabolism by strain JQ135. This study will provide insights into the bacterial catabolism of DPA.
研究领域:
微生物学
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环境科学
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生物化学
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2020
·
IF 10.652
Han Wang,Xinming Wu
As an emerging family of conductive 2D materials, MXenes, have gained much attention as electrode in supercapacitor devices. Their representative, Ti 3 C 2 T x , usually achieves significant capacitance in common acid electrolyte. In this work, through a simple dipicolinic acid (DC) intercalation strategy, Ti 3 C 2 T x -DC can obtain outstanding capacitance in a more environmentally friendly KCl aqueous electrolyte. As a consequence, as an electrode for supercapacitor, Ti 3 C 2 T x -DC exhibits an outstanding specific capacitance of 353 F g −1 at a current density of 0.5 A g −1 in KCl aqueous electrolyte (MXene-DC/KCl), which is higher than MXene of 320 F g −1 in H 2 SO 4 electrolyte (MXene/H 2 SO 4 ) and MXene of 127 F g −1 in KCl aqueous electrolyte (MXene/KCl). Moreover, the MXene-DC/KCl shows an impressive cycling stability with about 100% capacitance retention after 10,000 cycles at 1.0 A g −1 . Such excellent electrochemical performance in a greener electrolyte is mainly attributed to the interlayer hydration of DC and counter ion adsorption of electrolyte.
研究领域:
材料科学
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电化学
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储能
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DOI
高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·
IF 3.8
Xin-Yu Ji,Bing-Yi Wang,Yi-Feng Zhang,Yu-Jing Zhang,Ya-Jie Lai,Yang Yang,Xiang-Chen Wang,Su-Yan Wang,Pedro Laborda,Xin-Chi Shi
BACKGROUND Epicoccum sorghinum is a pathogenic fungus that causes leaf spot in a wide range of plants, including maize, and synthesizes the mycotoxin tenuazonic acid (TEA), which is carcinogenic. Despite the relevant economic and yield losses caused by E. sorghinum worldwide, methods for the control of this pathogen are lacking. RESULTS In this work, the efficacy of Bacillus -produced dipicolinic acid (DPA) for control of E. sorghinum was evaluated using in vitro and in vivo assays, and compared with the efficacy of three commercial fungicides, including carbendazim, prochloraz, and thiram. DPA inhibited E. sorghinum mycelial growth, and conidia germination, and produced important alterations in E. sorghinum hyphae. Interestingly, 10 mM DPA reduced TEA biosynthesis by 86.6%. Although DPA rapidly degraded on maize leaves, 10 mM DPA showed higher preventive (67.4% lesion length inhibition) and inhibitory (89.5% lesion length inhibition) efficacies for the control of E. sorghinum on maize leaves compared to the commercial fungicides. CONCLUSIONS Collectively, this study presents the first method for the control of E. sorghinum on maize and demonstrates that DPA application is a suitable approach to inhibit E. sorghinum symptoms in plants and TEA biosynthesis. © 2024 Society of Chemical Industry.
研究领域:
植物病理学
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农业科学
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毒理学
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DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 17
·4259
·
IF 4.2
Jing Li,Yu Liang,Chun Tian,Hongyan Zou,Lei Zhan,Lijuan Wang,Chengzhi Huang,Chunmei Li
Bacillus anthracisis a Gram-positive bacterium that can cause acute infection and anthracnose, which is a serious concern for human health. DeterminingBacillus anthracisthrough its spore biomarker dipicolinic acid (DPA) is crucial, and there is a strong need for a method that is rapid, sensitive, and selective. Here, we created Eu(III)-coordination polymers (Eu-CPs) with surfaces that have abundant carboxyl and hydroxyl groups. This was achieved by using citric acid and europium nitrate hexahydrate as precursors in a straightforward one-pot hydrothermal process. These Eu-CPs were then successfully utilized for highly sensitive DPA determination. The fluorescence (FL) emission of Eu-CPs, which is typically weak due to the coordination of Eu(III) with water molecules, was significantly enhanced in the presence of DPA. This enhancement is attributed to the competitive binding between DPA’s carboxyl or hydroxyl groups and water molecules. As a result, the absorbed energy of DPA, when excited by 280 nm ultraviolet light, is transferred to Eu-CPs through an antenna effect. This leads to the emission of the characteristic red fluorescence of Eu3+at 618 nm. A strong linear relationship was observed between the enhanced FL intensity and DPA concentration in the range of 0.5–80 μM. This relationship allowed for a limit of detection (LOD) of 15.23 nM. Furthermore, the Eu-CPs we constructed can effectively monitor the release of DPA from Bacillus subtilis spores, thereby further demonstrating the potential significance of this strategy in the monitoring and management of anthrax risk. This highlights the novelty of this approach in practical applications, provides a valuable determination technique for Bacillus anthracis, and offers insights into the development cycle of microorganisms.
研究领域:
微生物学
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分析化学
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生物医学
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DOI
PubMed
高影响因子
Biosensors-Basel
·
2022
·Vol. 12
·期 8
·668
·
IF 5.743
Xiang Li,Junsong Wu,Huaguang Hu,Fangfang Liu,Jialian Wang
A desirable lanthanide-based ratiometric fluorescence probe was designed as a multifunctional nanoplatform for the determination of dipicolinic acid (DPA), a unique bacterial endospore biomarker, with high selectivity and sensitivity. The carbon dots (CDs) with blue emission wavelengths at 470 nm are developed with europium ion (Eu3+) to form Eu3+/CDs fluorescent probes. DPA can specifically combine with Eu3+and then transfer energy from DPA to Eu3+sequentially through the antenna effect, resulting in a distinct increase in the red fluorescence emission peak at 615 nm. The fluorescence intensity ratio of Eu3+/CDs (fluorescence intensity at 615 nm/fluorescence intensity at 470 nm) showed good linearity and low detection limit. The developed ratiometric nanoplatform possesses great potential for application in complex matrices owing to its specificity for DPA. In addition, the integration of a smartphone with the Color Picker APP installed enabled point-of-care testing (POCT) with quantitative measurement capabilities, confirming the great potential of the as-prepared measurement platform for on-site testing.Keywords:ratiometric;fluorescence;determination of dipicolinic acid;smartphone-based detection
研究领域:
生物化学
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分析化学
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微生物学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Huihui Zhang,Qi Kang,Xuwei Chen
Anthrax is a serious zoonotic disease caused by Bacillus anthracis and dipicolinic acid (DPA) is the key component and major biomarker of Bacillus anthracis, therefore the development of rapid and sensitive DPA detection method is crucial to ensure public safety. In present study, carboxyl-functionalized covalent organic framework TpPa-COOH was synthesized using 2,5-diaminobenzoic acid and 2,4,6-triformylphloroglucinol as the building blocks, then Eu 3+ was immobilized onto TpPa-COOH via coordination interaction to give the fluorescent composite TpPa-COOH@Eu 3+ of dual-emissions. The presence of DPA significantly enhanced the characteristic emission of TpPa-COOH@Eu 3+ at 616 nm attributed by the “antenna effect” between DPA and Eu 3+ , thus a ratiometric fluorescent sensor was developed for the sensitive DPA detection with a detection limit of 9.5 nmol L −1 . The ratiometric fluorescent sensor offered DPA detection with high accuracy and favorable selectivity. The practicability of fluorescence sensor was validated by detecting DPA contents in real water samples. This research not only proposes a sensitive fluorescence method for anthrax biomarker detection, aiding in the prevention of biological threats and disease spread, but also highlights the potential of COFs in developing efficient ratiometric fluorescent probes with self-calibration capabilities.
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高影响因子
ANALYTICAL CHEMISTRY
·
2017
·
IF 6.32
Xiang-Ru Bai,Yi Zeng,Xiao-Dong Zhou,Xiao-Hua Wang,Ai-Guo Shen,Ji-Ming Hu
Field, reliable, and ultrasensitive detection of dipicolinic acid (DPA), a general biomarker of bacterial spores and especially Bacillus anthracis, is highly desirable but still challenging in current biometric security emergency response system. Herein we report an environmentally safe mercury(II) ions-mediated and competitive coordination interaction based approach for rationally designed surface-enhanced Raman scattering (SERS)-active gold nanoparticles (AuNPs), enabling rapid, ultrasensitive and zero-background detection of DPA without the pretreatment of samples. By means of competitiveness, these papain-capped gold nanoparticles (P-AuNPs) are induced to undergo controllable aggregation upon the addition of Hg2+ ions and DPA with a concentration range (1 nM∼8 μM), which correspondingly cause quantitative changes of SERS intensity of cresyl violet acetate (CVa) conjugated AuNPs. The decreased Raman intensity obtained by subtracting two cases of additives that contain only Hg2+ and the mixture of Hg2+ and DPA is proportional to the concentration of DPA over a range of 1 nM∼8 μM (R2 = 0.9824), with by far the lowest limit of detection (LOD) of 67.25 pM (0.01 ppb, S/N = 3:1). Of particular significance, mercury(II) ions actually play two roles in the process of measurements: a mediator for two designed competitive ligands (DPA and papain), and also a scavenger for the possibly blended ligands due to the different interaction time between DPA and the interferent with Hg2+ ions, which guarantees the interference-free detection of DPA even under real conditions.
研究领域:
分析化学
·
生物医学
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环境科学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 11
·1-9
·
IF 5.7
Xiong Ya,Yang Weiqiang,Huang Shengxiu,Chen Xiaoping,Wang Qingxiang,Ni Jiancong,Lin Zhenyu
Metal–organic gels (MOGs) emerged as an attractive luminescent soft material for electrochemiluminescence (ECL). In this work, a cathodic ECL-activated europium metal–organic gel (Eu-MOG) has been synthesized by a facile mixing of Eu 3+ with 4′-(4-carboxyphenyl)-2,2′:6′,2′′-terpyridine (Hcptpy) under mild conditions. The prepared Eu-MOG is highly mesoporous for co-reactant permeation to produce an ultra-stable and high-efficient ECL, based on the antenna effect of Eu 3+ coordinating with Hcptpy. Moreover, dipicolinic acid (DPA) can competitively coordinate with Eu 3+ instead of water molecules, producing an enhanced ECL signal. Therefore, an ECL enhancement assay was developed for DPA detection. There was a linear relationship between the ECL intensity and the logarithmic concentration of DPA in the 0.01–1 μM range, and the detection limit is 7.35 nM. This work displays the promising application of Eu-MOG in the ECL field, opening a broad inspection for seeking a new generation of ECL luminophores. Graphical Abstract
研究领域:
分析化学
·
材料科学
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PubMed
高影响因子
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