Journal of Food Measurement and Characterization
·
2025
·
IF 3.3
Jie Mingsha,Zhu Amei,Zhu Bing,Guo Ruipeng,Lan Shengkai,He Jiaxin,Lang Xingang,Bai Yanhong
The overuse of antibiotics leads to significant health risks and environmental challenges. Therefore, selective and sensitive detection of antibiotic residues is a critical issue. Here, a double-emission ratiometric fluorescence probe (Lu-Eu-DPA) was constructed for the selective detection of gentamicin sulfate (GEN), neomycin (NEO), and erythromycin (EM). The Lu-Eu-DPA coordination polymer nanoparticles composed of luminol, the coordinated nuclear metal Eu 3+ , and 2,6-pyridinedicarboxylic acid (DPA). The coordination reaction between luminol molecules and Eu 3+ ions produce the aggregation-induced emission (AIE) effect, while DPA acts as a ligand for the antenna, absorbing energy from the excitation light and then transferring the energy to Eu 3+ . Based on the AIE effect and antenna effect (AE), the fluorescence intensity of Lu-Eu-DPA at 426 nm and 617 nm was correlated with the concentration of GEN, NEO, and EM, and the detection limits of GEN, NEO, and EM were 20, 25, and 48 nmol/L, respectively. The detection capability of the proposed method in pork samples was also studied. In addition, semi-quantitative determination of GEN, NEO, and EM was conveniently and efficiently performed using a smartphone-based visualization method. These results suggested that this probe with good selectivity and specificity was a promising method for swiftly detecting multiple antibiotic residues.
研究领域:
分析化学
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食品安全
·
生物医学
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Yanxiang Zhang,Huiling Liu,Yanjun Xin,Yunpeng Shen,Jing Wang,Chen Cai,Mengmeng Wang
Erythromycin mycelial dreg (EMD) is a bio-fermentation residue of the pharmaceutical industry that may be used as a nutrient resource. However, the contaminants present in EMD need to be considered and controlled at an acceptable level prior to utilization. In this study, heat-activated persulfate (PS) oxidation of EMD was developed to degrade the residual erythromycin (ERY) and inactivate the ERY-resistant genes. The effects of temperature, initial PS concentration, and pH on the removal of ERY in EMD were studied. The results revealed that 99.0% ERY was degraded by 25 g PS/kg EMD under 80 °C within 120 min. The apparent rate constant ( k app ) in the acidic condition was higher than that in the alkaline condition. The contribution of sulfate radical ( SO 4 · - ) and hydroxyl radical (HO ) on ERY removal in EMD was found to be 36.8% and 29.4%, respectively. Six intermediate products (IPs) of ERY were clarified, and total organic carbon (TOC) was efficiently reduced by 99.4%. Finally, the detected ERY-resistant genes ere(B), erm(A), and erm(B) obviously presented a reduction during the heat-activated PS oxidation process.
研究领域:
环境科学
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微生物学
·
药学
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DOI
高影响因子
CHEMOSPHERE
·
2020
·
IF 5.778
Yixiao Wu,Liang Wan,Weihao Zhang,Huijun Ding,Wenfeng Yang
Here we report a set of experiments in which water blooming cyanobacteria Microcystis aeruginosa was repeatedly exposed to erythromycin . Growth inhibition increased with increasing erythromycin concentration (1–150 μg/L) upon first exposure. Maximum inhibition rate (76.06%), occurred under 150 μg/L erythromycin. Moreover, 96-h 50% effective concentration (EC 50 ) was 22.97 μg/L, indicating that the growth of M. aeruginosa was affected by erythromycin under common environmental concentrations. Photosynthesis was hindered by chlorophyll and photosystem II limitations. Malondialdehyde , reactive oxygen species , and superoxide dismutase contents increased significantly under certain concentrations of erythromycin, but superoxide dismutase was suppressed by 150 μg/L erythromycin. Synthesis of intracellular and extracellular microcystins was promoted by 10–60 and by 20–60 μg/L erythromycin, respectively, but both were inhibited by 100–150 μg/L. Principal component analysis and Pearson’s correlation revealed the accumulation of reactive oxygen species as the dominant mechanism of erythromycin toxicity to cells. M. aeruginosa repeatedly subjected to erythromycin exposure showed obvious resistance against the antibiotic , especially when treated twice with 60 μg/L erythromycin. The 96-h EC 50 was 81.29 μg/L. As compared to the first exposure to erythromycin, photosynthetic and antioxidant activities increased, while growth inhibition and oxidation stress decreased upon multiple exposures. Production and release of microcystins were enhanced by repeated exposure to the antibiotic. Thus, erythromycin persistence in water should be examined, as repeated exposure may lead to serious environmental and human health hazards.
研究领域:
环境毒理学
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藻类学
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水生生态学
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抗生素抗性
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水污染
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DOI
高影响因子
RSC Advances
·
2023
·Vol. 13
·期 27
·18651-18657
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IF 3.9
Jie Yu,Danwei Cen,Yongcheng Chen,Hailan Zhao,Mengyue Xu,Sulan Wu,Shuo Wang,Qiao Jin,Ting Shen
Antibiotic resistance is a big threat to public health. How to improve the therapeutic efficacy of conventional antibiotics is an effective way to address this issue. In order to enhance the antibacterial activity of conventional antibiotic erythromycin (EM), EM is conjugated to positively charged ε-poly-L-lysine (EPL) to obtain EPL modified EM (EPL-EM). The grafting ratio of EM can be calculated from the 1H NMR spectrum. EPL-EM is stable in physiological environment, while EM can be readily released from EPL-EM upon incubating with esterase which can be secreted by most bacteria. Because of the presence of cationic EPL, EPL-EM showed much stronger antibacterial activity than free EM, with much lower minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Moreover, compared to free EM, the development of drug resistance can be slowed down if EPL-EM is used, which can be ascribed to the reduction of EM dosage. Meanwhile, EPL-EM cannot induce hemolysis and cytotoxicity, which indicates that EPL-EM exhibits excellent biocompatibility. The design of EPL-EM with enhanced antibacterial activity and excellent biocompatibility provides an innovative way to combat antibiotic resistance.
研究领域:
药理学
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微生物学
·
治疗学
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DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2021
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IF 3.171
Yuanjie Li,Qiuxiang Yin,Meijing Zhang,Ying Bao,Baohong Hou,Jingkang Wang,Jiting Huang,Ling Zhou
Erythromycin thiocyanate is widely used for the production of other macrolide antibiotics. In this work, a novel heterosolvate of this pharmaceutical compound has been obtained and characterized for the first time, which was transformed from the dihydrate form in the acetone solvent through evaporation crystallization. Thermal behavior together with compositional analysis revealed that both water and acetone molecules participated in the formation of the crystal lattice which is rarely reported before. The general chemical name of the heterosolvate may be defined as erythromycin thiocyanate sesquihydrate hemiacetonate. Furthermore, studies on solid-state spectral analysis provided strong evidence of intermolecular hydrogen bonds in heterosolvate crystals. According to the crystal structure determined by single crystal X-ray diffraction, the formation mechanism of the heterosolvate is proposed in which strong multi-hydrogen bondings between water and solute molecules form the layer structure. While acetone molecules form single-hydrogen bonds with solutes and reside in channels between layers. This well explains why acetone solvent is easy to escape from the crystal structure during desolvation.
研究领域:
药学
·
晶体学
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产品关联
DOI
高影响因子
RESOURCES CONSERVATION AND RECYCLING
·
2020
·
IF 8.086
Chen Cai,Yu Hua,Huiping Li,Lei Li,Lingling Dai,Huiling Liu,Xiaohu Dai
Erythromycin fermentation residue (EFR) is a byproduct of bio-fermentation process for producing erythromycin, representing both an economic and environmental burden for the pharmaceutical industry. While hydrothermal treatment (HT) can solve the pollution and toxic problems, the impacts of HT on harmless performance and the bioresource properties of the complex EFR matrix remain unclear. In this study, the abatement of residual erythromycin, its associated antibiotic resistance genes and antimicrobial activity were investigated to evaluate the harmless performance of HT for EFR, and the feasibility of recycling hydrolysates as bioenergy was also assessed. The results showed that raising HT temperature significantly improved the harmless performance. The removal rate of residual erythromycin in EFR reached over 88% and the total absolute abundance of erythromycin resistance genes decreased by more than 3 logs with HT at 160°C for 60 min, but the hydrolysates still existed specific antimicrobial activity against S. aureus. . Additionally, the HT temperature remarkably influenced the bioresource properties of EFR-hydrolyzed products. The release of the protein-like polymers into the liquid phase was enhanced, while high HT temperature worsened the nutrient substances. The elevated drying property of solid products from EFR was observed along with the higher heating value of around 20 MJ/kg, indicating a potential of recycling solid products as biofuel. Total energy production from biogas conversion and biofuel incineration treating 1.0 kg raw EFR was 1875 kJ. Hydrothermal technology was proved to realize the harmless treatment of EFR and meanwhile to be promising for recycling EFR as bioenergy.
研究领域:
环境科学
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生物技术
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制药工程
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废物管理
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DOI
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 15
·3489
·
IF 4.2
Yanxiang Zhang,Chunxing Li,Xinyu Zhu,Irini Angelidaki
High-level erythromycin (ERY) fermentation wastewater will pose serious threats to lake environments. Anaerobic digestion (AD) has advantages in treating high-level antibiotic wastewater. However, the fate of antibiotic resistance genes (ARGs) and microbial communities in AD after stepwise exposure to high-level ERY remains unclear. In this study, an AD reactor was first exposed to 0, 5, 10, 50, 100 and 200 mg/L ERY and then re-exposed to 0, 50, 200 and 500 mg/L ERY to investigate the effect of ERY on AD. The results show that AD could adapt to the presence of high-level ERY (500 mg/L) and could maintain efficient CH4production after domestication with low-level ERY (50 mg/L). The AD process could achieve higher removal of ERY (>94%), regardless of the initial ERY concentration.ErmBandmefA, conferring resistance through target alteration and efflux pumps, respectively, were dominant in the AD process. The first exposure to ERY stimulated an increase in the total ARG abundance, while the AD process seemed to discourage ARG maintenance following re-exposure to ERY. ERY inhibited the process of acetoclastic methanogenesis, but strengthened the process of hydrogenotrophic methanogenesis. This work provides useful information for treating high-level ERY fermentation wastewater by the AD process.
研究领域:
环境科学
·
微生物学
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DOI
PubMed
高影响因子
Bioresources and Bioprocessing
·
2024
·Vol. 11
·期 1
·1-12
·
IF 4.3
Ke Xiang,Jiang Xing,Wang Shuohan,Tian Xiwei,Chu Ju
Comparative transcriptomics uncovered distinct expression patterns of genes associated with cofactor and vitamin metabolism in the high-yielding mutant strain Saccharopolyspora erythraea HL3168 E3, as compared to the wild-type NRRL 2338. An in-depth analysis was conducted on the effects of nine vitamins, and it was determined that thiamine pyrophosphate (TPP), vitamin B2, vitamin B6, vitamin B9, vitamin B12, and hemin are key enhancers in erythromycin production in E3, increasing the erythromycin titer by 7.96–12.66%. Then, the Plackett-Burman design and the path of steepest ascent were applied to further optimize the vitamin combination for maximum production efficiency, enhancing the erythromycin titer in shake flasks by 39.2%. Otherwise, targeted metabolomics and metabolic flux analysis illuminated how vitamin supplementation modulates the central carbon metabolism with notable effects on the TCA cycle and methionine synthesis to augment the provision of energy and precursors essential for erythromycin synthesis. This work highlights the capacity for precise vitamin supplementation to refine metabolic pathways, thereby boosting erythromycin production, and provides valuable directions for application on an industrial scale. Graphical
研究领域:
微生物学
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生物技术
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代谢工程
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DOI
PubMed
高影响因子
PHARMAZIE
·
2020
·Vol. 75
·期 9
·424-429
·
IF 1.198
Chen Jiangfei,Guo Suhang,Yu Xianzhu,Lei Jinxiu,Xu Tao,Zhu Suyan,Chen Lu,Xu Ping,Zhou Xuan,Yu Lushan
Flumatinib, indicated for the treatment of Philadelphia chromosome-positive chronic myeloid leukemia, is a structural analog of imatinib and has shown higher potency than imatinib as a BCR-ABL inhibitor. In this paper, the metabolic profile of flumatinib was studied. It was found that
CYP3A4 and CYP2C8 were the main cytochrome P450 enzyme substyles catalyzing the metabolism of flumatinib, and CYP3A4 has a stronger metabolic ability for flumatinib than CYP2C8. Erythromycin, cyclosporine, and voriconazole can inhibit the metabolism of flumatinib in vitro. Accordingly,
co-administration of erythromycin and cyclosporine with flumatinib increased the plasma concentration and the systemic exposure of flumatinib in rats, which indicated that lower doses should be considered in clinical practice.
研究领域:
药理学
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2023
·
IF 6.8
Kai Liu,Jiping Li,Yuhao Zhou,Wei Li,Hu Cheng,Jiangang Han
The ecological effects of antibiotics in surface water have attracted increasing research attention. In this study, we investigated the combined ecotoxicity of erythromycin (ERY) and roxithromycin (ROX) on the microalgae, Chlorella pyrenoidosa , and the removal of ERY and ROX during the exposure. The calculated 96-h median effect concentration (EC 50 ) values of ERY, ROX, and their mixture (2:1 w/w) were 7.37, 3.54, and 7.91 mg∙L -1 , respectively. However, the predicted EC 50 values of ERY+ROX mixture were 5.42 and 1.51 mg∙L -1 , based on the concentration addition and independent action models, respectively. This demonstrated the combined toxicity of ERY+ ROX mixture showed an antagonistic effect on Chlorella pyrenoidosa . During the 14-d culture, low-concentration (EC 10 ) treatments with ERY, ROX, and their mixture caused the growth inhibition rate to decrease during the first 12 d and increase slightly at 14 d. In contrast, high-concentration (EC 50 ) treatments significantly inhibited microalgae growth ( p < 0.05). Changes in the total chlorophyll contents, SOD and CAT activities, and MDA contents of microalgae suggested that individual treatments with ERY and ROX induced higher oxidative stress than combined treatments. After the 14-d culture time, residual Ery in low and high concentration Ery treatments were 17.75% and 74.43%, and the residual Rox were 76.54% and 87.99%, but the residuals were 8.03% and 73.53% in ERY+ ROX combined treatment. These indicated that antibiotic removal efficiency was higher in combined treatments than that in individual treatments, especially at low concentrations (EC 10 ). Correlation analysis suggested that there was a significant negative correlation between the antibiotic removal efficiency of C. pyrenoidosa and their SOD activity and MDA content, and the enhanced antibiotic removal ability of microalgae benefited from increased cell growth and chlorophyll content. Findings in this study contribute to predicting ecological risk of coexisting antibiotics in aquatic environment, and to improving biological treatment technology of antibiotics in wastewater.
研究领域:
环境科学
·
生物学
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产品关联
DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2022
·Vol. 106
·期 19
·6551-6566
·
IF 5.56
Khan Sabir,Xu Xueqi,Song Jialei,Wu Panpan,Liu Xiaobin,Liu Jing,Chen Ketao,Xu Zhenyue,Wu Hang,Zhang Buchang
Abstract TetR family transcriptional regulators (TFRs) are widespread in actinomycetes, which exhibit diverse regulatory modes in antibiotic biosynthesis. Nitrogen regulators play vital roles in modulation of primary and secondary metabolism. However, crosstalk between TFR and nitrogen regulator has rarely been reported in actinomycetes. Herein, we demonstrated that a novel TFR, SACE_4839, was negatively correlated with erythromycin yield in Saccharopolyspora erythraea A226. SACE_4839 indirectly suppressed erythromycin synthetic gene eryAI and resistance gene ermE and directly inhibited its adjacent gene SACE_4838 encoding a homologue of nitrogen metabolite repression (NMR) regulator NmrA (herein named NmrR). The SACE_4839-binding sites within SACE_4839-nmrR intergenic region were identified. NmrR positively controlled erythromycin biosynthesis by indirectly stimulating eryAI and ermE and directly repressing SACE_4839 . NmrR was found to affect growth viability under the nitrogen source supply. Furthermore, NmrR directly repressed glutamine and glutamate utilization-related genes SACE_1623 , SACE_5070 and SACE_5979 but activated nitrate utilization-associated genes SACE_1163 , SACE_4070 and SACE_4912 as well as nitrite utilization-associated genes SACE_1476 and SACE_4514 . This is the first reported NmrA homolog for modulating antibiotic biosynthesis and nitrogen metabolism in actinomycetes. Moreover, combinatorial engineering of SACE_4839 and nmrR in the high-yield S. erythraea WB resulted in a 68.8% increase in erythromycin A production. This investigation deepens the understanding of complicated regulatory network for erythromycin biosynthesis. Key points
SACE_4839 and NmrR had opposite contributions to erythromycin biosynthesis.
NmrR was first identified as a homolog of another nitrogen regulator NmrA.
Cross regulation between SACE_4839 and NmrR was revealed.
研究领域:
微生物学
·
分子生物学
·
生物技术
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DOI
PubMed
高影响因子
AQUATIC TOXICOLOGY
·
2024
·
IF 4.1
Jiping Li,Wei Li,Naisen Liu,Chenggong Du
The photodegradation products (PDPs) of antibiotics in the aquatic environment received increasing concern, but their chronic effects on microalgae remain unclear. This study initially focused on examining the acute effects of erythromycin (ERY), then explored the chronic impacts of ERY PDPs on Chlorella pyrenoidosa . ERY of 4.0 − 32 mg/L ERY notably inhibited the cell growth and chlorophyll synthesis. The determined 96 h median effective concentration of ERY to C. pyrenoidosa was 11.78 mg/L. Higher concentrations of ERY induced more serious oxidative damage, antioxidant enzymes alleviated the oxidative stress. 6 PDPs (PDP749, PDP747, PDP719, PDP715, PDP701 and PDP557) were identified in the photodegradation process of ERY. The predicted combined toxicity of PDPs increased in the first 3 h, then decreased. Chronic exposure showed a gradual decreasing inhibition on microalgae growth and chlorophyll content. The acute effect of ERY PDPs manifested as growth stimulation, but the chronic effect manifested as growth inhibition. The malonaldehyde contents decreased with the degradation time of ERY at 7, 14 and 21 d. However, the malonaldehyde contents of ERY PDPs treatments were elevated compared to those in the control group after 21 d. Risk assessment still need to consider the potential toxicity of degradation products under long-term exposure.
研究领域:
环境科学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）