Fenpyroximate is a systemic phenoxy pyrazole acaricide applied worldwide to prevent and control various phytophagous mites and has high activity against young mites, with residues increasingly being found in ecological environment and agricultural products. To identify its residues, four haptens of fenpyroximate were designed and an indirect competitive enzyme-linked immunosorbent assay (icELISA) based on monoclonal antibodies (2G4C7) was developed. Because the icELISA had higher sensitivity to the hydrolysate ( Hapten A ) of fenpyroximate, a method for indirectly determining the concentration of fenpyroximate was established by measuring the content of Hapten A . The assay had a working range of 0.07–1.49 ng/mL and a half-maximal inhibitory concentration (IC 50 ) of 0.34 ng/mL. It showed average recoveries of 77.0%–105.4 %, 72.3%–106.4 % in citrus and apple samples, respectively. The icELISA and UPLC-MS/MS test results for samples of various citrus cultivars are remarkably consistent. These results and data represent the icELISA is suitable and applicable for detecting fenpyroximate residuals in fruit and vegetable samples.
Yumei Yan,Zhili Liu,Wenfeng Zhou,Haixiang Gao,Runhua Lu
Herein, three patterns for the detection of fenpyroximate based on the response signal of gold nanoparticles are described. The strong interaction between the guanidine group of arginine-modified gold nanoparticles and the ester group of fenpyroximate led to the aggregation of the nanoparticles and to a variation of ultraviolet-visible light spectrum and color of the solution. Sensors were constructed based on the correlation of the concentration of fenpyroximate with the absorbance ratio (A650/A525) and the R value was obtained by extracting the color of the test solution by using a smartphone to take a photo of the solution, which was then analyzed by colorimeter software. The absorbance ratio increased linearly in the range of 0.225–0.375 mg L−1 and the limit of detection was 0.215 mg L−1, while the R value declined linearly in the range of 0.20–0.40 mg L−1 and the limit of detection was 0.21 mg L−1. Further, the gold nanoparticles could cause a fluorescence quenching of fluorescent dyes, such as rhodamine B, and it was found that the fluorescence could be quenched and then restored after aggregation; therefore, a fluorescence method based on fluorescence “off–on” was constructed, and the fluorescence quenching was found to increase linearly in the range of 0.0–1.0 mg L−1 and the limit of detection was 0.013 mg L−1. These three patterns indicated highly selective and sensitive response signals for fenpyroximate, and all were applied to the detection of fenpyroximate in apple juice, pear juice, and environmental water samples, with the results showing that the three methods could be mutually verified, with the recoveries ranging from 94.15% to 110.65%.
危险品化学品经营许可证（带存储）