There is a growing interest in the utilization of animal by-products and wastes. Plant-derived enzymes could play a vital role in collagen extraction from tannery-trimming wastes. Here, the ficin enzyme from fig leaves was used for collagen extraction and compared with conventional acetic acid and pepsin extraction methods, aiming to assess the efficiency and purity of collagen extracted using this plant-based enzyme approach relative to the organic solvents and animal-derived enzymes. The extracted collagen was identified as type I collagen. Response surface methodology analysis revealed that the ficin enzyme-soluble collagen yielded 15.28 % at a hydrolysis time of 39.27 h, a ficin enzyme dose of 5.54 %, and a mixing ratio of 15.87, higher than the acetic acid-soluble collagen yield of 9.52 % and pepsin enzyme-soluble collagen yield of 14.56 %. Ficin enzyme-soluble collagen exhibited better integrity of the triple helical structure with fewer proteinaceous impurities, though it showed poorer thermal stability. Ultrasound treatment at 800 W for 30 min did not significantly disrupt the triple helical structure of collagen. The work aims to develop a green and sustainable method for extracting collagen from tannery-trimming wastes, offering a novel approach for the high-value conversion of animal by-products and agro-residues wastes.
Pan Yadi,Pang Yanjiao,Shi Ying,Zheng Wen,Long Yijuan,Huang Yuming,Zheng Huzhi
An enzyme-metal organic framework (MOF) composite with saucer-like structure was prepared via one-pot synthesis from ficin (a cysteine proteolytic enzyme with POx activity), zinc(II) ions and 2-methylimidazole. The composites exhibit a 2.5-fold higher catalytic activity and stronger affinity for substrates compared to free ficin. This was exploited to design a colorimetric assay for the determination of glucose. The addition of glucose oxidase causes the formation of H 2 O 2 which is catalytically oxidized by ficin to form a blue coloration that can be measured at 652 nm. The assay has a 0.12 μM detection limit and excellent selectivity. It was successfully applied to the determination of glucose in diluted serum samples.
Schematic presentation of the synthesis process and the enhanced peroxidase activity of ficin@MOF composites. Ficin was immobilized in a new saucer-like shape of Ficin@MOF composites, which showed higher peroxidase activity than free ficin. Scheme and graphical abstract contains poor quality of text.We have attach a new picture with 600 dpi as scheme and graphical abstract.
危险品化学品经营许可证（带存储）