Scientific Reports
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2017
·Vol. 7
·期 1
·1-8
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IF 4.259
Yang Yufang,Shen Dongjun,Long Yijuan,Xie Zhixiong,Zheng Huzhi
Ficin is classified as a sulfhydryl protease isolated from the latex of fig trees. In most cases, a particular enzyme fits a few types of substrate and catalyzes one type of reaction. In this investigation, we found sufficient proofs for the intrinsic peroxidase-like activity of ficin and designed experiments to examine its effectiveness in a variety of scenarios. Ficin can transform peroxidase substrates to colored products in the existence of H 2 O 2 . Our results also indicate that the active sites of peroxidase-like activity of ficin are different from that of protease, which reveals that one enzyme may catalyze more than one kind of substrate to perform different types of reactions. On the basis of these findings, H 2 O 2 releasing from MCF-7 cells was detected successfully. Our findings support a wider application of ficin in biochemistry and open up the possibility of utilizing ficin as enzymatic mimics in biotechnology and environmental monitoring.
研究领域:
生物化学
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酶学
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生物技术
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环境监测
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2017
·
IF 2.536
Yanjiao Pang,Zili Huang,Yufang Yang,Yijuan Long,Huzhi Zheng
In this work, we developed a colorimetric biosensing system for glucose detection by coupling the peroxidase-like of ficin and the glucose oxidase (GOx). GOx can catalyze the oxidation of glucose to produce H 2 O 2 , then, ficin catalyzes the oxidation of peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB) by H 2 O 2 to produce a blue color reaction. The present sensing system showed a linear response toward glucose detection over range of 2.0–100 μM with a detection limit of 0.5 μM. This system is simple, low cost, highly sensitive and selective for glucose detection, and was also applied to measuring glucose in human serum. Furthermore, in order to expand the application of ficin in biological sensing, we immobilized ficin onto the SiO 2 @Fe 3 O 4 NPs, which exhibited the merits of recycling as well as allowing the repeated detection of glucose. Thus it may provide great potential applications in biomedicine, biotechnology and environmental chemistry .
研究领域:
生物医学
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分析化学
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生物技术
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DOI
PubMed
高影响因子
Analytical Methods
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2018
·Vol. 10
·期 23
·2676-2680
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IF 2.073
Zili Huang,Yufang Yang,Yijuan Long,Huzhi Zheng
The concentration of L-cysteine (L-Cys) provides important information about specific physiological functions and disease diagnosis. In this work, we provided a facile colorimetric approach for L-Cys detection with high sensitivity and specificity. Ficin acted as a peroxidase mimic and catalyzed the chromogenic reaction of TMB and H2O2. However, after the introduction of L-Cys, L-Cys competed with TMB for H2O2. The competition is generally won by the more-reactive L-Cys resulting in fading of the chromogenic reaction. The detection limit of L-Cys can be as low as 20 nM (3σ). In addition, the assay can also be successfully applied to detect L-Cys in biological fluids, indicating its potential in biological sample analysis. Our work could promote the application of the enzyme mimicking activity of ficin in medical diagnostics and biochemistry.
研究领域:
生物化学
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医学诊断
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酶学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Qijue Chen,Yumeng Wang,Xiangning Wu,Zhuoer Jiang,Boxian Zhang,Mǎdǎlina Georgiana Albu Kaya,Kheng Lim Goh,Keyong Tang
There is a growing interest in the utilization of animal by-products and wastes. Plant-derived enzymes could play a vital role in collagen extraction from tannery-trimming wastes. Here, the ficin enzyme from fig leaves was used for collagen extraction and compared with conventional acetic acid and pepsin extraction methods, aiming to assess the efficiency and purity of collagen extracted using this plant-based enzyme approach relative to the organic solvents and animal-derived enzymes. The extracted collagen was identified as type I collagen. Response surface methodology analysis revealed that the ficin enzyme-soluble collagen yielded 15.28 % at a hydrolysis time of 39.27 h, a ficin enzyme dose of 5.54 %, and a mixing ratio of 15.87, higher than the acetic acid-soluble collagen yield of 9.52 % and pepsin enzyme-soluble collagen yield of 14.56 %. Ficin enzyme-soluble collagen exhibited better integrity of the triple helical structure with fewer proteinaceous impurities, though it showed poorer thermal stability. Ultrasound treatment at 800 W for 30 min did not significantly disrupt the triple helical structure of collagen. The work aims to develop a green and sustainable method for extracting collagen from tannery-trimming wastes, offering a novel approach for the high-value conversion of animal by-products and agro-residues wastes.
研究领域:
生物化学
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环境科学
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可持续发展
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2018
·
IF 5.667
Yanjiao Pang,Ying Shi,Yadi Pan,Yufang Yang,Yijuan Long,Huzhi Zheng
Polydopamine nanoparticles (pDA NPs), a kind of popular polymer material, have been broadly applied to various areas owing to its excellent physicochemical properties. In this work, we employed ficin as a peroxidase-mimic to in situ synthesize intrinsic fluorescent pDA NPs and developed a fluorescence turn-on method for rapid dopamine (DA) sensing which is based on monitoring the intrinsic fluorescence of pDA NPs. Ficin possesses intrinsic peroxidase-like activity that could catalyze the oxidization of dopamine to its quinone derivative and autopolymerize into fluorescent pDA NPs by H 2 O 2 . Thus, the fluorescence intensity of pDA NPs gradually enhanced with increasing concentration of DA. Under the optimum conditions, the strategy showed a good linear relationship from 10 nM to 5.0 μM ( R 2 = 0.996) and provided an exciting detection limit of 5.5 nM. More importantly, the presented sensing approach has high sensitivity, good selectivity and has been successfully applied to DA sensing in human serum samples. With its good analytical figures of merit, our proposed analytical method is promising for the detection of DA-related diseases.
研究领域:
分析化学
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生物医学工程
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材料科学
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DOI
高影响因子
MICROCHIMICA ACTA
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2019
·Vol. 186
·期 4
·1-8
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IF 5.479
Pan Yadi,Pang Yanjiao,Shi Ying,Zheng Wen,Long Yijuan,Huang Yuming,Zheng Huzhi
An enzyme-metal organic framework (MOF) composite with saucer-like structure was prepared via one-pot synthesis from ficin (a cysteine proteolytic enzyme with POx activity), zinc(II) ions and 2-methylimidazole. The composites exhibit a 2.5-fold higher catalytic activity and stronger affinity for substrates compared to free ficin. This was exploited to design a colorimetric assay for the determination of glucose. The addition of glucose oxidase causes the formation of H 2 O 2 which is catalytically oxidized by ficin to form a blue coloration that can be measured at 652 nm. The assay has a 0.12 μM detection limit and excellent selectivity. It was successfully applied to the determination of glucose in diluted serum samples.
Schematic presentation of the synthesis process and the enhanced peroxidase activity of ficin@MOF composites. Ficin was immobilized in a new saucer-like shape of Ficin@MOF composites, which showed higher peroxidase activity than free ficin. Scheme and graphical abstract contains poor quality of text.We have attach a new picture with 600 dpi as scheme and graphical abstract.
研究领域:
生物化学
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材料科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2020
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IF 3.232
Yijuan Long,Wen Zheng,Danyang Yi,Yadi Pan,Huzhi Zheng
Ficin has dual enzyme activity , i.e., protease and peroxidase-like activity. In some respects, its application is limited by the protease activity of ficin. Herein, we used tris (2-carboxyethyl) phosphine hydrochloride (TCEP) to break the three pairs of disulfide bonds of ficin, and then blocked the free thiol groups with N-ethylmaleimide (NEM) to synthesize ficin-TN. The results showed that ficin-TN had increased peroxidase-like activity and reduced protease activity. According to this phenomenon, we have exploited a colorimetric method with high sensitivity and selectivity for the one-step detection of glucose. Comparing with ficin, ficin-TN has wider detection range (0.1–300 μM) and lower detection limit (88 nM), and our method is simpler and more timesaving than other two-step methods. Furthermore, the actual appliances of ficin-TN for glucose detection in human serum have been illustrated with satisfied result, suggesting that its promising utilization in various fields.
研究领域:
生物化学
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分析化学
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酶学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2020
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IF 3.232
Wen Zheng,Jiahui Liu,Danyang Yi,Yadi Pan,Yijuan Long,Huzhi Zheng
Ficin has been reported to possess peroxidase activity, but its applications in some respects have been limited because of its relatively low activity. Herein, a mesoporous metal-organic framework, PCN-333(Fe), was synthesized, which was selected to encapsulate ficin to form [email protected] (Fe). Compared with ficin, the peroxidase-like activity of [email protected] (Fe) toward 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation was about 3 times increase in the presence of H 2 O 2 , and followed classical Michaelis-Menten model. The kinetic parameters showed that stronger affinity and higher catalytic constant ( K cat ) of [email protected] (Fe) to both TMB and H 2 O 2 compared with ficin, and K cat of [email protected] (Fe) was increased by 3.65 folds and 3.59 folds for TMB and H 2 O 2 , respectively. Taking advantages of higher catalytic property of [email protected] (Fe), we developed a colorimetric method with high sensitivity and selectivity to detect glucose, which displayed a good linear response toward glucose in the range of 0.5–180 μM with a limit of detection of 97 nM. Furthermore, [email protected] (Fe) has been proven to successfully detect glucose in human serum, implying its great potentialities and wide applications as peroxidase mimics.
研究领域:
生物化学
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分析化学
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材料科学
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DOI
PubMed
高影响因子
Food Chemistry-X
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2025
·
IF 8.2
Zi-Yue Wang,Yun-Zhe Ma,Xin-Yue Jiang,Chen-Ying Wang,Ying-Qiu Li,Yan Liang,Xi-Dong Ren,Li-Cheng Qin,Xiang-Zhong Zhao
Thermal processing could reduce tenderness of cooked tuna. Papain, bromelain and ficin were employed to solve this issue, and their tenderizing mechanisms were investigated. Protein profiles showed that macromolecular proteins in tuna were degraded into smaller fractions by proteases, which led to increases in the myofibrillar fragmentation index (MFI) values. All proteases reduced shear force and hardness but increased cooking loss of tuna (except ficin), accompanied by the formation of unevenly distributed pores in tuna microstructures. Compared with control, bromelain decreased shear force and hardness by 86.95 % and 58.25 %, showing the best tenderizing effect. Moreover, reduction in content of disulfide bonds, the primary molecular force in tuna network, also contributed to the improvement of tenderness. Additionally, the ordered structures (α-helix and β-sheet) were transformed to disordered structures (β-turn and random coil) by proteases. These findings showed that proteases could significantly enhance the tenderness of tuna, especially bromelain.
研究领域:
食品科学
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生物化学
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蛋白质组学
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质构分析
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酶学
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DOI
PubMed
高影响因子
TALANTA
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2018
·
IF 4.244
Yadi Pan,Yufang Yang,Yanjiao Pang,Ying Shi,Yijuan Long,Huzhi Zheng
Ficin, a classical sulfhydryl protease, was found to possess intrinsic peroxidase-like activity. In this paper, we have put forward a novel strategy to improving the peroxidase-like activity of ficin through binding heme. Heme-ficin complexes were successfully obtained by simple one-step syntheticism. The results demonstrated that the catalytic activity and efficiency of heme-ficin complexes were about 1.7 times and 3 times higher than those of native ficin, respectively. Taking advantages of the high peroxidase-like activity, the heme-ficin complexes were used for colorimetric determination of uric acid with a low detection limit of 0.25 μM. Based on the excellent selectivity and sensitivity, we detected the concentration of uric acid in human serum successfully. On the basis of these findings, the heme-ficin complexes are promising for wide applications in various fields. Thus we not only optimized the peroxidase-like activity of the ficin, but also established a new strategy for development of artificial enzyme mimics by mimicking the architecture of the active site in horseradish peroxidase .
研究领域:
生物化学
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分析化学
·
酶学
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DOI
PubMed
高影响因子
TALANTA
·
2019
·
IF 4.916
Wen Zheng,Dongjun Shen,Yadi Pan,Danyang Yi,Yijuan Long,Huzhi Zheng
The peroxidase-like activity of ficin is relatively low, which limits its application. It was found that thiol groups of ficin could inhibit its peroxidase-like activity. So, two procedures, i.e., direct blocking with N -ethylmaleimide (NEM), or using tris (2-carboxyethyl) phosphine hydrochloride (TCEP) to interrupt disulfide bonds then blocking thiol groups with NEM, were applied to block thiol groups of ficin, ficin-NEM (ficin-N) and ficin-TCEP-NEM (ficin-TN) were produced, respectively. The blocking of thiol groups accelerated the peroxidase activity dramatically. The peroxidase catalytic activity of ficin-N and ficin-TN toward the peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation by H 2 O 2 was about 2.5-fold and 5-fold increase compared with ficin, respectively, which accompanied a color change from colorless to blue and followed classic Michaelis-Menten model. The kinetic parameters indicated that higher affinity of ficin-N ( K m = 0.31) and ficin-TN ( K m = 0.39) to H 2 O 2 compared with ficin ( K m = 0.58), and ficin-TN had the highest K cat which increased by 6.5 times and 4.5 times for TMB and H 2 O 2 , respectively. According to these findings, a colorimetric method with high sensitivity for the detection of biothiols was developed due to sulfhydryl compounds inhibited the peroxidase activity of ficin. Comparing with ficin and ficin-N, ficin-TN had the widest detection range (0.01–16 μM) and the lowest detection limit (3 nM). The practical applications of ficin-TN for biothiol determination in human serum samples have been demonstrated with satisfactory results. Ficin-N and ficin-TN are promising to apply to the bioanalysis.
研究领域:
生物化学
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分析化学
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生物技术
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2018
·
IF 2.88
Ying Shi,Yanjiao Pang,Ning Huang,Chaoqun Sun,Yadi Pan,Yuan Cheng,Yijuan Long,Huzhi Zheng
Dopamine (DA), a catecholamine neurotransmitter , is considered to be an important indicator for mental diseases detection in the clinic. In this study, a novel fluorescent sensing platform consisting of the ficin-H 2 O 2 -tyramine system for determining DA in cerebrospinal fluids (CSF) was established. The proposed method is based on the fact that ficin, a mimetic peroxidase , can catalyze H 2 O 2 decomposition into OH radicals, which can oxidize non-fluorescent tyramine into fluorescent dityramine. When DA was introduced, DA can compete with tyramine for OH and resulting in the oxidation reaction of tyramine inhibited along with the fluorescence intensity of the system decreased, which provides a unique strategy for fluorescence detection of DA. Under optimal conditions, the fluorescence intensity decreased linearly with the DA level over a wide concentration range from 0.05 to 12.0 μM (R 2 = 0.995) with a detection limit of 46 nM (3σ/k). More importantly, the proposed sensing approach exhibits high sensitivity, good selectivity and has been successfully applied to DA sensing in complex biological samples, which made it hold great potential for DA determination in chemical and biological analytical applications.
研究领域:
分析化学
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神经化学
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生物分析化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）