International Journal of Electrochemical Science
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2023
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IF 1.5
Bin Li,Ruizhi Zhang,Fanfan Du
The monitoring of fermentation processes is an important topic in the food industry. In this work, the method of electrochemical fingerprint was proposed to detect the fermentation process of sour bamboo shoots. At the same time, the contents of total acid, amino acid nitrogen, total sugar and flavonoid of bamboo shoots at different fermentation stages were also measured. The changes of electrochemical fingerprints reflected the changes of flavonoids in the fermentation process of bamboo shoots to some extent. The electrochemical fingerprint data were processed with first and second derivatives. These data were modeled by BP neural network and RBF neural network respectively to train the data of different fermentation stages. The results show that BP neural network is more suitable for identifying different fermentation stages of bamboo shoots.
研究领域:
食品科学
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电化学
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生物化学
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高影响因子
INDUSTRIAL CROPS AND PRODUCTS
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2014
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IF 3.208
Xian Xia,Jianguo Cao,Yunxia Zheng,Quanxi Wang,Jianbo Xiao
Ferns are famous traditional medicinal plants and 300 fern species have been used as phytomedicines by Southwestern of China. However, there is little information on the phytochemicals and pharmacological characteristics of most fern species. The total flavonoid concentrations, antioxidant activity, anticancer activity, and acetylcholinesterase inhibition of extracts from 19 species of ferns were investigated. The total flavonoid concentrations ranged from 8.6 to 306.4 mg/g (w/w). The antioxidant activity including DPPH free radical scavenging, ABTS radical scavenging, and superoxide anion scavenging, reducing power and ferric reducing antioxidant potential (FRAP) of flavonoid extracts from these ferns showed a weak linear relationship with their total flavonoid concentrations. Selaginella frondosa with very low total flavonoid concentration (13.4 mg/g) showed highest inhibition against A549 cells and it is necessary to further investigate the anti-cancer compounds in S. frondosa . The extracts from Davallia cylindrica Ching and Stenoloma chusanum Ching significantly inhibited AChE activity, which illustrated that it is worthy of attention to investigate flavonoids from D. cylindrica and S. chusanum as AChE inhibitors.
研究领域:
植物化学
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药理学
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生物活性研究
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DOI
高影响因子
Horticulture Research
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2024
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IF 7.6
Yuan Ziyu,Li Gu,Zhang Huixian,Peng Zhaoxin,Ding Wenyu,Wen Huan,Zhou Hanxin,Zeng Jiwu,Chen Jiajing,Xu Juan
Citrus fruits have abundant flavonoid glycosides (FGs), an important class of natural functional and flavor components. However, there have been few reports about the modification of UDP-glycosyltransferases (UGTs) on flavonoids in citrus. Notably, in flavonoid biosynthesis, 7-O-glucosylation is the initial and essential step of glycosylation prior to the synthesis of flavanone disaccharides, the most abundant and iconic FGs in citrus fruits. Here, based on the accumulation of FGs observed at the very early fruit development stage of two pummelo varieties, we screened six novel flavonoid 7-O-glucosyltransferase genes (7GlcTs) via transcriptomic analysis and then characterized them in vitro. The results revealed that four Cg7GlcTs possess wide catalytic activities towards various flavonoid substrates, with CgUGT89AK1 exhibiting the highest catalytic efficiency. Transient overexpression of CgUGT90A31 and CgUGT89AK1 led to increases in FG synthesis in pummelo leaves. Interestingly, these two genes had conserved sequences and consistent functions across different germplasms. Moreover, CitUGT89AK1 was found to play a role in the response of citrus to Huanglongbing infection by promoting FG production. The findings improve our understanding of flavonoid 7-O-glucosylation by identifying the key genes, and may help improve the benefits of flavonoid biosynthesis for plants and humans in the future.
研究领域:
植物科学
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生物化学
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分子生物学
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
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2023
·Vol. 47
·期 29
·14046-14055
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IF 3.3
Meng Sun,Yuyang Wang,Anni Huang,Yuxin Tian,Runqi Yang,Hanlu Wang,Xinmei Zhao,Xinqiang Song
Constructing supramolecular interactions between small molecules and polymers is emerging as a powerful strategy for developing sophisticated nanostructures with excellent performance. Herein, by varying the concentration of baicalein, a continuous bead-free and naturally-derived polyvinyl alcohol (PVA)/sodium alginate (SA) based nanofiber was fabricated through electrospinning and physical crosslinking. Fabrication of a hydrophobic fiber with antioxidant (baicalein) storage is realized by connecting a one-step integration procedure with physical post-modification. Physically crosslinked PVA and SA are used as hydrophobic and hydrophilic segments, respectively, for supramolecular interactions dominated by hydrogen bonds and immobilizing the antioxidant baicalein. The addition of SA can provide the stable interactions and result in a soft film with little bifurcated microstructure. Baicalein is used not only as an antioxidant but also to tune the morphology of the resulting bead-free fibrous film. Concurrently, the bead-free film increases the water resistance and the survival rate of UV protection by approximately double under continuous UV radiation compared to PVA–SA film. With regard to the above results, the current work develops a facile process for the production of materials with sophisticated nanostructures in the fields of clothing or packaging.
研究领域:
材料科学
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生物医学工程
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高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
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2025
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IF 3.7
Chen-Xin Guo,Jiyan Li,Yu Wang,Tang Li,Heng Yin
Stevia rebaudiana , a perennial herb, is recognized not only for its sweet steviol glycosides but also for its rich flavonoid content, which confer pharmacological properties including anti-inflammatory, antimicrobial, and anticancer activities. However, the enzymatic basis underlying flavonoid modification in S. rebaudiana remains poorly understood. In this study, we identified, cloned, and heterologously expressed a novel flavonoid glycosyltransferase gene, SrUGT72B1 in E. coli . The recombinant SrUGT72B1 catalyzed the glycosylation of multiple flavonoids using UDP-glucose as the primary sugar donor, and exhibited broad substrate promiscuity toward apigenin, luteolin, phloretin and kaempferol. In addition to UDP-glucose, SrUGT72B1 also accepted UDP-xylose and UDP-rhamnose, with UDP-glucose exhibiting the highest catalytic efficiency. Biochemical characterization revealed that the enzyme functions optimally at pH 9.0 and 50 °C. Notably, SrUGT72B1 demonstrates regioselective 5-O-glycosylation toward apigenin, a rare activity among plant glycosyltransferases. Molecular docking and molecular dynamics simulations provided structural insights into this unique regioselectivity and substrate recognition. Together, these findings establish SrUGT72B1 as a previously uncharacterized flavonoid 5-O-glycosyltransferase, expanding the functional landscape of plant UGTs and offering potential applications in the biosynthesis of value-added flavonoid glycosides.
研究领域:
植物学
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生物化学
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分子生物学
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药理学
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PubMed
高影响因子
FITOTERAPIA
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2024
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IF 2.5
Meihong Liu,Yongxia Jia,Haihui Xie
Michelia champaca L. (Magnoliaceae) was cultivated in large scale for flowers as cosmetic raw materials, whereas the value of its leaves remains to be discovered. Our chemical study on the leaves yielded four new flavonol diglycosides, champaflavosides A–D ( 1 – 4 ), together with twenty-three known flavonoid glycosides ( 5 – 27 ). Their structures were determined by spectroscopic and chemical methods. Compounds 5 – 21 and 23 – 27 were not previously reported from the genus Michelia , and kaempferol 3- O -rutinoside ( 22 ) was obtained from this species for the first time. All the compounds were evaluated for antioxidant activity by four in vitro assays. Compounds 3 – 12 and 20 showed more potent 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity than l -ascorbic acid ( l -AA). Compounds 2 – 23 , 25 , and 27 exhibited 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical cation scavenging activity superior to l -AA. The ferric reducing antioxidant powers (FRAP) of compounds 2 – 13 , 17 , and 19 were higher than l -AA. Further, eighteen compounds demonstrated cellular reactive oxygen species (ROS) scavenging activity, of which champaflavoside D ( 4 ), rhamnetin 3- O -neohesperidoside ( 8 ), quercetin 3- O -(6- O - E - p -coumaroyl)-neohesperidoside ( 9 ), and liquiritin ( 27 ) were more potent than curcumin. The results revealed that the renewable leaves of M. champaca are a rich source of flavonoids and antioxidants.
研究领域:
药用植物学
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生物化学
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植物化学
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DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
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2024
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IF 11
Xitong Liu,Fengzhang Wang,Jirong Wang,Ting Liu,Chengying Zhao,Jinkai Zheng
Pectin and flavonoids are important components of citrus fruits, but the effects of flavonoid grafting on the emulsifying properties of citrus pectin remain unclear. In this study, a novel natural citrus flavonoid-pectin conjugate in citrus peel was identified through alkaline hydrolysis combined with ultra-high performance liquid chromatography. Subsequently, two major citrus flavonoids were enzymatically conjugated to citrus pectin at high grafting rates (hesperidin: 20.21%; naringin dihydrochalcone: 16.11%). The flavonoids increased pectin amphipathy while ensuring a tighter conformation and denser network structure. These structural changes reduced the interfacial tension (from 20.6 to 18.4 mN/m) and increased the adsorbed layer thickness (from 11.8 to 37.8 nm), facilitating rapid pectin anchoring at the oil-water interface. These led to smaller droplet size and better emulsion stability. Prepared flavonoid-pectin conjugates with high grafting rates exhibited better emulsifying properties than those with medium grafting rates; hesperidin-pectin conjugates were more effective than naringin dihydrochalcone-pectin conjugates. The prepared flavonoid-pectin conjugates aid in the structural identification of natural flavonoid-pectin conjugates; they also facilitate analyses of the properties of these conjugates and offer new insights into improving the functional properties of pectins used in the food industry.
研究领域:
食品科学
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化学
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生物技术
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DOI
高影响因子
Journal of Functional Foods
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2017
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IF 3.144
Xuchao Jia,Dan Yang,Haihui Xie,Yueming Jiang,Xiaoyi Wei
The fruit of Averrhoa carambola L. (Oxalidaceae) is a commercially important fruit. Our study on the chemical constituents present in the fresh sweet fruit led to the isolation of eleven non-flavonoid phenolic compounds. Their structures were identified by analyses of spectroscopic data. They were two new alkyl phenol diglucosides, carambolasides K ( 1 ) and L ( 2 ), four phenylpropanoids, (+)-isolariciresinol 9- O -β- d -glucoside ( 3 ), (+)-lyoniresinol 9- O -β- d -glucoside ( 4 ), (−)-lyoniresinol 9- O -β- d -glucoside ( 5 ), and 1- O -feruloyl-β- d -glucose ( 6 ), three benzoic acids, protocatechuic acid ( 7 ), 1- O -vanilloyl-β- d -glucose ( 8 ), and tecomin ( 9 ), a simple phenol, koaburaside ( 10 ), and a naphthoquinone, (+)-cryptosporin ( 11 ). All of them were reported from A. carambola fruit for the first time. Compounds 1 – 8 and 10 showed 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical cation scavenging activity comparable to l -ascorbic acid, while compounds 3 , 5 , and 7 exhibited potent ferric reducing antioxidant power (FRAP). In addition, compounds 6 and 11 demonstrated weak porcine pancreatic lipase inhibitory activity.
研究领域:
植物化学
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药理学
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食品科学
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DOI
高影响因子
Plant Diversity
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2020
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IF 1.864
Xing Huang,Weiqi Li,Xudong Zhang
Exploring approaches to regulate meristem is of special importance and broad interest. In this study, we found that the flavonoid scutellarin, which has a 6-hydroxyl and a 7-glucoside, increased root length through the transcription factor NUTCRACKER (NUC). This root lengthening disappeared in NUC -knockout and reappeared in NUC -rescue plants. Scutellarin induced NUC expression and promoted the division of cortex/endodermal initials. In contrast, naringenin, which has same chemical backbone but without 6-hydroxyl and with 7-hydroxyl group, showed the opposite or no effects. Our results demonstrate that scutellarin promotes root length through NUC-mediated regulatory pathways and reveal that flavonoids with and without the 6-hydroxyl and 7-glucoside have positive and negative effects on meristem size, respectively.
研究领域:
植物生物学
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分子遗传学
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发育生物学
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DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL RESEARCH
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2015
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IF 0.633
Qian Wang,Wei Cui,Jian Yang,Bo Yang
Two novel routes for the synthesis of the flavonoid chrysin are described. In the first, 3,5-dimethoxyphenol (taxicatigenin) was converted to 2-hydroxy-4,6-dimethoxyacetophenone and then by condensation with benzaldehyde to 2′-hydroxy-4′,6′-dimethoxychalcone. The latter was cyclised with iodine and demethylated with pyridine hydrochloride to form chrysin in 50% overall yield. In the second route, taxicatigenin was acylated with cinnamoyl chloride to form a chalcone under special conditions. This was then converted to chrysin in 42% and 56% overall yield, respectively. Several disadvantages of previous syntheses like long reaction time, tedious work-up and low overall yield have been overcome.
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高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2025
·
IF 2.8
Changhai Sun,Mengxin Xiao,Biyue Cui,Liting Mu,Shuting Zhang,Xinran Zhang,Shushuang Shen
In the current research on Scutellaria baicalensis, a substantial number of flavonoid isomers were identified. Given the limitations of existing analytical methods to accurately identify these isomers, the present study was conducted to determine the flavonoid isomers in Scutellaria baicalensis by combining the mass spectrometry database and stepwise multiple linear regression QSRR model of flavonoids established by the research group in the previous phase. The UHPLC-Q-Exactive Orbitrap-MS technique with isocratic elution injection was employed, and compositional analysis of Scutellaria baicalensis was conducted utilizing the mass spectrometry database to identify two groups of flavonoids and their isomers. The QSRR model was calibrated using standards, and the two groups of flavonoids and their isomers in Scutellaria baicalensis were distinguished and identified utilizing the QSRR model and calibration equation. In this investigation, two groups of flavonoids and their isomers in Scutellaria baicalensis were successfully identified and differentiated. The findings suggest that this QSRR model can be utilized to identify flavonoid isomers, providing a viable method for differentiating isomers of chemical components.
研究领域:
植物化学
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分析化学
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药学
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产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
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2026
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IF 9.2
Xiaolan Wu,Mingyu Li,Shikui Zhang,Beibei Feng,Yulu Liu,Feiyan Gu,Li Yin,Wenwen Li
Flavonoids are important secondary metabolites that contribute to the nutritional quality and sensory characteristics of apricot fruit; however, their variation across cultivars and fruit tissues remains insufficiently characterized. Following an initial survey of total flavonoid content across 123 apricot accessions, four cultivars with contrasting levels were selected for UPLC–MS/MS profiling of the peel and flesh. In total, 760 flavonoid metabolites were identified and quantified, most of which accumulated at higher levels in the peel, indicating that the peel is a major flavonoid-rich tissue in these cultivars. Multivariate analysis identified 35 and 34 key differentially accumulated metabolites in the flesh and peel, respectively. Cultivar-specific differences involved distinct flavonoid classes: ‘Luotuohuang’ (LTH) was enriched in proanthocyanidin A2*, whereas ‘Zixing’ (ZX) preferentially accumulated rhamnosylated and acetylated flavonoid glycosides. These findings characterize tissue- and cultivar-specific flavonoid variation and highlight valuable flavonoid resources for apricot breeding and peel by-product utilization.
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高影响因子
危险品化学品经营许可证（带存储）