CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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2021
·
IF 1.134
Ke-Xin WANG,Chen NIE,Rui-Fang GUAN,Hao ZHANG
In recent years, unconventional fluorescent molecules without conjugated rings have attracted widespread interest due to their excellent fluorescence properties, simple preparation process, good water solubility and excellent biocompatibility. Dual emission fluorescent probes are usually more accurate and sensitive, for eliminating the influence of environmental factors. This work provides a novel strategy to construct double emission fluorescent probes based on unconventional fluorescent molecules and fluorescein isothiocyanate. The aqueous solution of resulting double emission polymer dots ( f-PDs ) shows two fluorescence emission peaks centered at 440 nm and 510 nm under excitation at 363 nm. According to change of these two peaks after adding ions, Cu 2+ and ClO − are judged quantitatively. Cu 2+ or ClO − are also quench fluorescence of f-PDs linearly, as Cu 2+ combines with polyethyleneimine parts of f-PDs to form complexes, while ClO − oxidize hydroxyl groups on the surface of f-PDs . The detection limits of Cu 2+ and ClO − , in an aqueous solution are 6.7 nM and 14.0 nM, respectively.
研究领域:
分析化学
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生物医学
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DOI
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 13
·7732-7737
·
IF 3.361
Fenying Wang,Dan Wang,Tingting Wang,Yu Jin,Baoping Ling,Qianjin Li,Jianlin Li
Fluorescent molecularly imprinted polymers (FMIPs) are gaining increasing attention in analytical and medical sciences, particularly silica-based FMIPs due to their low cost, environmentally friendly nature and good biocompatibility. However, at present, silica-based FMIPs are usually prepared through several steps and displayed low selectivity. Here, a simple approach was utilized for preparing silica-based FMIP nanoparticles. The polymerization was initiated by 3-aminopropyltriethoxysilane (APTES), which also acted as the functional monomer in the imprinting system; in addition, to achieve one-pot synthesis, a fluorescent monomer was prepared by a simple reaction between fluorescein isothiocyanate (FITC) and APTES. The as-synthesized FMIP nanoparticles displayed high specificity and fast response time (<1 min) towards the target molecule. Environmental pH and buffer salt could affect the specific recognition behaviors of the FMIP nanoparticles. Such a simple catalyst-free synthetic technique could also be employed for the preparation of FMIP nanoparticles targeting other acidic molecules.
研究领域:
分析化学
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材料科学
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医学
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DOI
PubMed
高影响因子
ChemistrySelect
·
2024
·Vol. 9
·期 40
·e202403141
·
IF 1.9
Changhua Su,Yan Lin,Anqi Xing,Like Dou,Weifang Han
Graphical Non-rare earth MBNNSs/FITC composite phosphor with high-intensity fluorescence emission was obtained via the incorporation of FITC into MBNNSs. The enhancement of the emission intensity of FITC in the composite phosphor can be attributed to the reduced dye aggregation via the confinement of FITC within MBNNSs, as well as the efficient energy transfer from MBNNSs host to FITC dyes. In addition, the MBNNSs/FITC composite phosphor exhibits excellent thermal and photo stabilities. Organic luminogens play an indispensable role in optoelectronic devices, yet there is a pressing need to address their aggregation-caused quenching (ACQ) and stability. Here we designed and prepared multilayered boron nitride nanosheets/fluorescein isothiocyanate (MBNNSs/FITC) composite phosphors with tunable photoluminescence by controlling the FITC content and annealing temperature. The research results indicate that MBNNSs/FITC with low or high loading of FITC is not conducive to enhancing the emission intensity of FITC, which can be attributed to the fact that low loading leads to a decrease in the content of fluorescent groups, while high loading promotes intermolecular aggregation of FITC dyes. Interestingly, the emission intensity of FITC in MBNNSs/FITC-6 composite phosphors can be further enhanced by annealing treatment (200 °C), which can be ascribed to the suppression of the ACQ and effective energy transfer from the MBNNSs to the FITC. Moreover, the resultant composite phosphor displays outstanding thermal stability, remarkable photo stability and exceptional water-resistance. This work provides a new design space for the development of non-rare-earth fluorescent materials using organic fluorescent dyes.
研究领域:
材料科学
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光学
·
化学
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DOI
高影响因子
Journal of Analytical Science and Technology
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2023
·Vol. 14
·期 1
·1-8
·
IF 2.4
Xu Ting,Qingru Zeng,Fu Qing,Wang Zhaojie,Liu Xin,Xiao Shensheng,Jiang Xiaoming,Lu Yuepeng,Gong Zhiyong,Wu Yongning,Fang Min
Using amino silica modified with fluorescein isothiocyanate (FITC), a quick fluorescence analysis technique is used for the detection of 3-monochloropropane-1,2-diol (3-MCPD). At 100 °C and pH 8.5, FITC-modified amino silica exhibits the lowest fluorescence intensity in the presence of 3-MCPD. This can predominantly be explained mostly explained by 3-MCPD’s capacity to occupy the amino group that FITC normally binds to. The fluorescence intensity of FITC-modified amino silica was greatly quenched by 3-MCPD’s reaction with the amino group under alkaline conditions, and the fluorescence intensity is different at different reaction times, reaction pH, and reaction temperature. The effects of various 3-MCPD concentrations on the optical characteristics of FITC-modified amino silica were also investigated. Fluorescence analysis is used to obtain a linear range from 0.025 to 1.0 mg/L for 3-MCPD detection under optimal experimental conditions, with a detection limit of 0.025 mg/L and a correlation coefficient of 0.9915. The quantity of 3-MCPD in soy sauce was measured under ideal conditions. Using the optimized conditions, the contents of 3-MCPD in soy sauce were determined. These results suggest that this method is sensitive to 3-MCPD and may have a substantial application in the rapid detection of food contaminants particularly, where the quality and safety of food products are of paramount concern.
研究领域:
分析化学
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食品科学
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纳米技术
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DOI
高影响因子
JOURNAL OF THE CHINESE CHEMICAL SOCIETY
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2023
·Vol. 70
·期 10
·1908-1915
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IF 1.8
Raheel Akram,Anila Arshad,Muhammad Saqib
Background and purpose The distinction of tannic acid (TA) in the presence of other analogues, i.e., gallic acid (GA), dopamine (DA), glucose (Glu), and fructose (Fruc), containing vicinal diols, has long been a significant yet challenging task due to their similar chemical structures and minuscule levels. Methods Here, the integration of 3-aminophenyl boronic acid-modified fluorescein isothiocyanate (3-APBA-FITC) for pH-dependent ultrasensitive and selective detection of TA in wines was proposed. By performing fluorescence signal off and pH-dependent boronate-ester bond synthesis, the suggested probe may specifically identify TA. Results The as-prepared 3-APBA-FITC designed probe had shown ultrahigh sensitivity with a limit of detection (LOD) of 0.151 μM. Finally, in detecting TA in artificial wine with a recovery between 92.49-93.05 % and a relative standard deviation of no more than 2.0 %, the designed strategy has been successfully applied. Conclusion Therefore, the envisaged sensing strategy can be an interesting option for discerning detection of diols containing analytes in food products.
研究领域:
分析化学
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食品科学
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生物传感
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2016
·
IF 2.653
Huabing Liu,Chaoyong Xu,Yanli Bai,Lin Liu,Dongmei Liao,Jiangong Liang,Lingzhi Liu,Heyou Han
Carbon dots (CDs) have been widely used for the preparation of multifunctional probes by conjugation with organic fluorescent dyes . However, the effect of organic fluorescent dyes on CDs still remains poorly understood. Herein, the effect of fluorescein isothiocyanate (FITC) on CDs was explored by spectroscopic techniques at pH 5.1, 7.0 and 9.0. The fluorescent intensity of CDs was found to be quenched gradually after mixing directly with different concentrations of FITC, but the fluorescent lifetime of CDs remained unchanged. According to the results of UV–vis absorption spectra and fluorescent lifetime measurements, a pH-dependent inner filter effect (IFE) between CDs and FITC was proposed. However, the fluorescent lifetime of CDs deceased after their conjugation with FITC, implying the fluorescence resonance energy transfer (FRET) between CDs and FITC. This study has revealed two different effects of FITC on CDs with varying pH values and provided useful theoretical guidelines for further research on the interaction between other nanoparticles and fluorophores.
研究领域:
生物化学
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纳米生物学
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光学
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
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2015
·Vol. 39
·期 9
·7363-7371
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IF 3.086
Wen-Yong Hu,Huan Liu,Yuan-Zhi Shao
In this study, four bi-modal core-shelled contrast agents of SiO2(FITC)@Gd2O(CO3)2·H2O with varying shell thicknesses but the same cores have been prepared via a two-step wet chemistry method. The four samples were quite close to sphericity and their averaged diameters were 127, 140, 148.7 and 174.0 nm. The composition of their shells was also examined. As the shell thickness increased, the fluorescence intensity halved but the longitudinal relaxivity remained high (28, 29, 27, 24 mM−1 s−1). The measured relaxivities of the samples were more than 5.8 times higher than the relaxivity of Gd-DTPA-BMA in a 0.55 T MR (magnetic resonance) system. The levels of cytotoxicity were tested on four cell lines (293, HeLa, HFL1, MCF-7) and the results indicated that the samples were safe. The intracellular fluorescence in HeLa cells was clearly observed using laser confocal microscopy. These results showed that this material had potential for application in MR-fluorescence bi-modal imaging.
研究领域:
生物医学工程
·
材料科学
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DOI
高影响因子
BIOPHARMACEUTICS & DRUG DISPOSITION
·
2018
·Vol. 39
·期 6
·298-307
·
IF 1.677
E Zhang,Fulong Chu,Lixu Xu,Hao Liang,Shuliang Song,Aiguo Ji
A new method to label fucoidan sulfate was established with tyramine and fluorescein isothiocyanate isomer I (FITC). Fluorescence spectrophotometry and high performance liquid chromatography verified the successful labelling of fucoidan by FITC. The results of the single-pass intestinal perfusion indicated that the jejunum and ileum are the main absorption sites, and there was carrier saturation. In addition, fucoidan sulfate at 1 mg/ml had no inhibitory effect on Caco-2 cell proliferation. Studies on the transmembrane transport mechanism showed that fucoidan can be absorbed because the apparent permeability coefficient of the drugs (P app ) A → B was 3.78 + 0.03 ×10 −6 and that of B → A was 1.42 + 0.19 ×10 −6 . The peak absorption of fucoidan occurred at 120 min after administration; moreover, the higher the concentration used, the worse the absorption was, suggesting the saturation of transport carriers. The absorption was temperature dependent: the absorption at 37°C was much better than that at 4°C. Further, the absorption of fucoidan sulfate might rely on clathrin endocytosis as chlorpromazine (10 μg/ml) significantly inhibited it.
研究领域:
药理学
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生物化学
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分子生物学
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2015
·
IF 2.752
Shasha Wang,Xiaokun Wang,Zhiyang Zhang,Lingxin Chen
An innovative fluorescence method for sensitive detection of copper ion (Cu 2+ ) was developed based on fluorescein isothiocyanate functionalized gold nanoparticles (FITC-AuNPs). Due to the stronger binding affinity of isothiocyanate functional group to gold, FITC molecules could adsorb on the surface of AuNPs, forming a simple fluorescence resonance energy transfer (FRET) system, and the fluorescence intensity of FITC was remarkably quenched. Upon adding cysteine, FITC could be displaced from the surface of AuNPs because the formation constant ( K f ) of Au–S linkage ( K f (AuS − ) = 4 × 10 35 ) was much higher than Au–SCN linkage ( K f (Au(SCN) 2− ) = 10 23 ), leading to the recovery of fluorescence intensity. However, Cu 2+ could catalyze O 2 oxidation of cysteine, and the generated disulfide cystine could not remove FITC from AuNPs’ surface. Therefore, the recovery of fluorescence intensity was much weaker when compared with that of in the absence of Cu 2+ . And on the basis of this principle the concentration of Cu 2+ could be detected quantitatively. Under optimal conditions, our method exhibited high selectivity toward Cu 2+ and provided a good linear relationship in the range of 1.0–17.0 nM with the detection limit of 0.37 nM calculated by 3 σ / S . Furthermore, complicated synthetic procedures and poor water solubility could be ignored in this proposed fluorescent sensor.
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高影响因子
TALANTA
·
2016
·
IF 4.035
Xinxin Zhu,Hui Jin,Cuili Gao,Rijun Gui,Zonghua Wang
In this article, a facile aqueous synthesis of carbon dots (CDs) was developed by using natural kelp as a new carbon source. Through hydrothermal carbonization of kelp juice, fluorescent CDs were prepared and the CDs’ surface was modified with polyethylenimine (PEI). The PEI-modified CDs were conjugated with fluorescein isothiocyanate (FITC) to fabricate CDs-FITC composites. To exploit broad applications, the CDs-FITC composites were developed as fluorescent sensing or imaging platforms of pH and Cu 2+ . Analytical performances of the composites-based fluorescence (FL) sensors were evaluated, including visual FL imaging of pH in glass bottle, ratiometric FL sensing of pH in yogurt samples, visual FL latent fingerprint and leaf imaging detection of [Cu 2+ ], dual-signal FL sensing of [Cu 2+ ] in yogurt and human serum samples. Experimental results from ratiometric, visual, dual-signal FL sensing and imaging applications confirmed the high feasibility, accuracy, stabilization and simplicity of CDs-FITC composites-based FL sensors for the detection of pH and Cu 2+ ions in real samples.
研究领域:
分析化学
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材料科学
·
生物医学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 7
·1-11
·
IF 5.3
Li Huiru,Tian Yanbo,Tan Liju,Wang Na,Qiao Yu,Wang Jiangtao
A novel molecularly imprinted ratiometric fluorescent sensor CQDs@MIP/FITC@SiO 2 for the detection of p-nitroaniline (p-NA) was constructed through the mixture of CQDs@MIP and FITC@SiO 2 in the ratio of 1:1 ( V CQDs@MIP : V FITC@SiO₂ ). The polymers of CQDs@MIP and FITC@SiO 2 were prepared by sol-gel method and reversed-phase microemulsion method, respectively. CQDs@MIP was used as the auxiliary response signal and FITC@SiO 2 was used as the reference enhancement signal. The signal was measured at excitation/emission wavelengths of 365/438, 512 nm. The sensor showed good linearity in the concentration range 0.14–40.00 µM ( R 2 = 0.998) with a detection limit of 0.042 µM for p-NA. The color change of “blue-cyan-green” could be observed by the naked eye under 365 nm UV light, thus realizing the visual detection of p-NA. The sensor presented comparable results compared with high-performance liquid chromatography (HPLC) method for the detection of p-NA in hair dye paste and aqueous samples with recoveries of 96.8–103.7% and 95.8–104.4%, respectively. It was demonstrated that the constructed sensor possesses the advantages of simplicity, excellent selectivity, superior sensitivity, and outstanding stability. Graphical Abstract
研究领域:
分析化学
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材料科学
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环境科学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）