RSC Advances
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2023
·Vol. 13
·期 41
·28550-28559
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IF 3.9
Xiaohua Xu,Huye Li,Yapeng Sun,Tianfeng Ma,Lin Shi,Wencheng Mu,Huan Wang,Yongchang Lu
Cr3+ is one of the most essential trace elements in living organisms and plays a vital role in human metabolism. However, both deficiency and excess intake of Cr3+ can be harmful to the human body. Therefore, the quantitative determination of Cr3+ is of great significance in the field of life science. Based on this, in this study, a g-CNQDs@p-acetaminophenol fluorescence sensing system was developed for the quantitative detection of Cr3+ in actual complex samples. G-CNQDs were synthesized with sodium citrate and urea as precursors. The fluorescence signal was enhanced by the synergistic effect between p-acetaminophenol (APAP) and g-CNQDs. The fluorescence quenching phenomenon can be produced when Cr3+ is introduced into the fluorescence-enhanced g-CNQDs@p-acetaminophenol system. An “on–off” fluorescence sensing system was constructed based on g-CNQDs@p-acetaminophenol for the quantitative detection of Cr3+. The experimental data showed a wide linear region in the concentration range of 0.64–63.0 μM, and the detection limit was as low as 0.23 μM. The construction of the sensor system broadens the research field for the practical application of Cr3+.
研究领域:
分析化学
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生物医学工程
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环境科学
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SEPARATION AND PURIFICATION TECHNOLOGY
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2022
·
IF 9.136
Ruolan Li,Xingyan Liu,Yacen Tang,Jianning Yu,Ya Chen,Min Fu,Siqi Li,Youzhou He
In this study, a highly efficient bifunctional porphyrin-based MOFs with uniformly in-situ generated g-C 3 N 4 quantum dots (g-CNQDs) was fabricated for photocatalytic hydrogen evolution and NO removal, in which PtTCPP as the organic ligand and mixed Zr/Ti-oxo clusters as the metal nodes. Compared with most reported Zr-based porphyrin MOFs, the g-CNQDs@Zr-MOFs(Pt)(Zr/Ti) exhibited outstanding hydrogen evolution activity (14511.57 μmol g −1 ) and NO removal rate (31.9 %) under visible light irradiation. The obviously improved photocatalytic performance could be attributed to the significantly enhanced charge separation and transfer rates by the mixed Zr/Ti-oxo clusters via the rapid Ti 3+ /Ti 4+ process. Moreover, the uniformly in-situ generated g-CNQDs via confinement effect in the cavity of MOFs could also improve the utilization of visible light especially through the up-conversion effect, and promote charge carriers separation. This work provides an idea for us to expand the scope of light utilization and promote charge transfer and separation rates of MOFs via functionalizing host MOFs matrix and coupling guest active species to improve photocatalytic performance further.
研究领域:
材料科学
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环境科学
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化学
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高影响因子
MICROCHIMICA ACTA
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2026
·Vol. 193
·期 3
·126
·
IF 5.4
Zhou Yu,Wu Runqiang,Fan Lingling,Qui Pengtao,Deng Xiaohua,Tan Xiaohong,Dong Shuaikang,Li Canru,Chen Jiejing,Liang Jintao,Li Guiyin
Hepatocellular carcinoma (HCC) is one of the most lethal malignancies worldwide, and early diagnosis is crucial. Golgi protein 73 (GP73) has emerged as a promising serum biomarker for HCC. However, current detection methods often fail to meet routine screening requirements due to limitations in sensitivity and operational simplicity. To address these challenges, we have developed a novel fluorescent aptamer-based sensor for highly sensitive GP73 detection based on the fluorescence resonance energy transfer (FRET) mechanism between graphitic carbon nitride quantum dots (g-CNQDs) and a copper-based metal–organic framework (Cu-TCPP). g-CNQDs were covalently conjugated with a GP73-specific aptamer to serve as the fluorescent donor, while two-dimensional Cu-TCPP nanosheets acted as the efficient acceptors. Fluorescence was quenched upon donor–acceptor interaction via FRET. In the presence of GP73, aptamer–target binding disrupted FRET interaction, separating the donor from the acceptor and restoring fluorescence in a concentration-dependent manner. Under optimal conditions, the sensor exhibited excellent linearity over a concentration range 1.0–225.0 ng mL⁻¹, with a detection limit as low as 0.907 ng mL⁻¹. Recoveries for spiked human serum samples ranged from 95.96% to 103.85%, with relative standard deviations (RSDs) of 0.38%–5.48%. The developed aptamer sensor demonstrated excellent sensitivity, selectivity, and stability, providing a powerful tool for early HCC diagnosis and offering strong potential for real-time analytical applications. Graphical A novel fluorescent aptamer sensor for GP73 detection by leveraging the fluorescence resonance energy transfer (FRET) mechanism between graphite-phase carbon nitride quantum dots (g-CNQDs) and a copper-based organic framework (Cu-TCPP). This platform achieved a good linear relationship with GP73 concentration in the range of 1.0–225.0 ng mL -1 for GP73 concentration, and a detection limit of 0.907 ng mL -1 .
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2020
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IF 3.232
Shasha Feng,Fubin Pei,Yi Wu,Jingjing Lv,Qingli Hao,Tinghai Yang,Zhaoyang Tong,Wu Lei
A novel ratiometric fluorescent sensor based on Förster resonance energy transfer (FRET) platform was designed for riboflavin (RF) detection. The graphitic carbon nitrides quantum dots - Zn-MOF composite (g-CNQDs@Zn-MOF) was used as the fluorescent probe . In the FRET system, g-CNQDs@Zn-MOF and RF acted as donor and acceptor, respectively. The probe exhibited high sensitivity and good selectivity to RF, and had been successfully used for the detection of RF in milk and vitamin B 2 tablets. The detection limit of the sensor was 15 nM. The strategy expanded the application of MOF in sensing filed and provided a new method for the detection of RF.
研究领域:
分析化学
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生物医学工程
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BIOSENSORS & BIOELECTRONICS
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2017
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IF 7.78
Zhuoyue Zhang,Zhen Zhang,Huihui Liu,Xiang Mao,Wei Liu,Shouting Zhang,Zongxiu Nie,Xiaoquan Lu
Visual inspection is expected as an ideal technique, which can directly and conveniently detect heavy metal ions by observing the color change. Insensitivity of detecting weakly colored heavy transition metal ions and low adsorptivity of metal ions on nanoparticle surface are two main factors hindering the application of visual detection in heavy metal ions detection. Herein, we demonstrated an operational colorimetric sensor based on the color dependence of nanoparticles aggregation to selective and facile detect weakly colored transition heavy metal Cd 2+ ions that have been considered as the origin of the “Itai-itai” disease. Uniform colloidal 15 nm graphite-like nitride doped carbon quantum dots-capped gold nanoparticle (Au@g-CNQDs) was successfully prepared, wherein the existence of numerous heptazine, carboxyl and hydroxyl groups on the nanoparticle's surface strengthened adsorption of the Cd 2+ ions on the surface of Au@g-CNQDs through the “cooperative effect”. As a consequence, without expensive and intricate exogenous indicators or other special additives, the Cd 2+ ions could sensitively and quickly captured to detect at ultra-low concentration within 30 s by the naked-eye. Under the optimal conditions, the Cd 2+ ions sensor possesses good analytical performances with a wide linear range of 0.01–3.0 μM and a detection limit of 10 nM (S/N = 3). Moreover, the biodistribution and aggregation of Cd 2+ ions were detected effectively in mice organ tissues suggesting its great potential use for real-word applications.
研究领域:
环境科学
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纳米医学
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生物化学
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高影响因子
ANALYTICAL CHEMISTRY
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2018
·
IF 6.042
Yanyan Yu,Yuan Yang,Jinhua Ding,Si Meng,Chenglin Li,Xiaoxing Yin
Circulating tumor cells (CTCs) served as an important biomarker for tumor recurrence and prediction of prognosis. However, selective capture and quantification of CTCs from whole blood was still full of challenge due to the extremely scare number of CTCs. Moreover, how to keep a high cell viability after capture remained to be solved. Here, we described a ratiometric fluorescent probe for the efficient capture and accurate determination of CTCs by conjugating graphitic carbon nitride quantum dots (g-CNQDs) with gold nanoclusters (AuNCs) and further linking with anti-EpCAM antibody to acquire the CTC-specific immune probe. In this probe, AuNCs protected by albumin V bovine played the role as the fluorophore reference and anti-EpCAM-attached g-CNQDs acted as both the response signal and specific recognition element for sensing CTCs. In the presence of CTCs, the quenched fluorescence of the immune probe at 500 nm was recovered due to the detachment of anti-EpCAM from the probe, whereas the intensity at 650 nm was essentially unchanged. This strategy realized the highly sensitive detection of CTCs in whole blood down to one CTC. Furthermore, it was demonstrated that the designed probe allowed capturing living CTCs with minimal cell damage. The subsequent reculture of captured cells for proliferation revealed that after a 7 day proliferation, almost 28 MCF-7 cells were obtained from one target cell. The immune probe was successfully applied into capture and detection of CTCs from clinical cancer patients. Our data suggested the good potential of fluorescent probe for the clinical diagnosis of cancers.
研究领域:
生物医学工程
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纳米技术
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癌症生物学
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分子诊断
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高影响因子
TALANTA
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2022
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IF 6.556
Zhimin Liu,Jie Wang,Chen Cui,Limei Zheng,Leqian Hu
An ultrasensitive resonance energy transfer based “on-off” switch electrochemiluminescence (ECL) immunosensing strategy was proposed and applied for prostate specific antigen (PSA) detection. In detail, self-enhancing nanohybrids of nano-CeO 2 and AuNPs decorated graphitic carbon nitride quantum dots (CeO 2 –AuNPs–g-CNQDs) were synthesized, which produced high ECL emission with coreactant K 2 S 2 O 8 for “switch-on” state. Noticeably, due to the reversible Ce 3+ ↔ Ce 4+ reaction, nano-CeO 2 could serve as highly-efficient coreaction accelerator and induce the decomposition of S 2 O 8 2− into SO 4 • - , which endowed the g-CNQDs/K 2 S 2 O 8 system with enhanced ECL emission. Moreover, AuNPs acted crucial role in promoting the electronic transmission rate and providing sites to immobilize the biomolecules. On the other hand, the AgNPs-VB 2 nanocomposites were used as dual labels to quench the ECL signal of g-CNQDs-K 2 S 2 O 8 system via ECL-RET, leading to the decreased ECL for “switch-off” state. On the basis of sandwich-typed immunosensing strategy, the developed ECL immunosensing platform exhibited sensitive response to PSA in a linear range of 1.0 × 10 −5 _ 50 ng mL −1 with a low detection limit of 0.0045 pg mL −1 ( S/N = 3). This work provided a promising tool in early clinical diagnostics of prostate cancer.
研究领域:
生物医学工程
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分析化学
·
肿瘤学
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高影响因子
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