Yu-Xiao Wang,Yue Xin,Xiao-Jun Huang,Jun-Yi Yin,Shao-Ping Nie
A homogeneous galactoglucan was purified from the alkali-extracted polysaccharides from the basidioma of Macrolepiota albuminosa by gradient ethanol precipitation, whose proposed structure was given for the first time. Results showed it had a molecular weight of 210 kDa, and mainly consisted of glucose and galactose. There were abundant filaments, randomly distributed sheet-like and flaky appearance in its surface by SEM observation. Its backbone comprised β-(1 → 6)-Glc p , α-(1 → 6)-Gal p and β-(1 → 3,6)-Glc p residues at 4:1:1, terminated by β-(1 → 3)-Glc p and T -Glc p residues. Rheological measurements suggested its steady flow behavior was highly dependent on concentrations. Newtonian behavior was evident at low concentrations, whereas pseudoplastic behavior was observed at high concentrations. Besides, the X-ray diffraction patterns proved the presence of amorphous structure. The conformational parameters were detected by HPSEC-MALLS-RI, revealing a random coil conformation in NaNO 3 aqueous solution. This work provides a theoretical basis for the application of polysaccharides from M. albuminosa in food- and drug-based therapies.
Yue Cui,Aocheng Liu,Kaixin Hu,Heping Sun,Junpeng Hu,Dan Qin,Jing Li
Cutaneous wound healing remains a significant clinical challenge. Although natural polysaccharide-based hydrogels are promising due to their biocompatibility and bioactivity, the potential of rhizobial galactoglucans in wound management has been underexplored. In this study, a Sinorhizobium -derived galactoglucan (EPSII), consisting of equimolar galactose and glucose in the linkage pattern →3)-β-D-Glc p -(1→3)-(4,6-Pyr)-α-D-Gal p -(1→ and possessing a weight-average molecular weight of 1.97 × 10 6 Da, was crosslinked with polyethylene glycol diglycidyl ether (PEGDGE) to form hydrogels. By modulating the ratios of polymer to crosslinker, we obtained hydrogels with tunable porosity, high swelling capacity (up to 531.3 ± 13.4%), strong adhesion (up to 101.3 ± 1.5 kPa), excellent water retention, degradation resistance, and suitable rheological properties. The hydrogels were stretchable, residue-free upon removal, and retained structural stability after autoclaving. The hydrogel with formulation G5P1.5 (porosity 82.3 ± 3.2%) was selected for further assay. The G5P1.5 hydrogel exhibited minimal cytotoxicity and low hemolysis (<1.8 ± 0.1%). In a murine full-thickness wound model, G5P1.5 significantly enhanced wound closure, reducing the wound area to 3.2 ± 1.8% by day 12 post injury. Mechanism studies indicated that the hydrogel downregulated pro-inflammatory cytokines, promoted collagen deposition, and boosted antioxidant enzyme activities, outperforming a commercial hydrogel dressing in several aspects. These findings demonstrate the potential of Sinorhizobium galactoglucan hydrogels as effective wound dressings and highlight the value of rhizobial polysaccharides in biomedical applications.
Zhou Xu,Yanxia Xiong,Pei Hu,Long Chen,Jianhua Wan,Chenggang Huang,Wenjun Liu
Alpinia officinarumHance has a medicinal history of thousands of years in treating cough, diabetes, and gastrointestinal system diseases, and it is also a medicine food homology (MFH) plant in China. To evaluate the pharmacological activities of polysaccharides from the rhizomes ofA. officinarum, polysaccharides were initially obtained by hot-water extraction and the ethanol precipitation method. A homogenous polysaccharide designated as AOP-w was isolated by a DE-52 column. The proposed structure was elucidated and the immunoregulatory effects on RAW 264.7 macrophage cells were evaluated. The results showed that AOP-w had a molecular weight of 5.26 kDa, and mainly consisted of galactose and glucose (molar ratio of 0.12:0.88). Its backbone comprised α-(1→4)-Glcp, α-(1→4,6)-Glcpand β-(1→3,4)-Galpresidues, terminated by α-(1→6)-Glcpand T-Glcpresidues. AOP-w was nontoxic to RAW 264.7 cells, but demonstrated promotion in cell proliferation within a 100 μg/mL concentration. The immunostimulatory effects of AOP-w were confirmed by the elevated NO production of AOP-w-treated cells. Moreover, the RNAseq was conducted and the results showed that AOP-w may activate the TNF and NF-κB signaling pathways by binding to Toll-like receptors, thereby affecting the immune modulatory activity of RAW264.7 cells. These results suggest a high potential of AOP-w fromA. officinarumfor immunotherapeutic applications.
危险品化学品经营许可证（带存储）