Uncontrolled hemorrhage resulting from traumas causes severe health risks. There is an urgent need for expeditious hemostatic materials to treat bleeding incidents. Here, we developed a natural protein-based hemostatic sponge extracted from nonmulberry cocoons that exhibited rapid coagulation and effective absorption. We first built a degumming and dissolution system suitable for the Dictyoploca japonica cocoons to obtain regenerated silk fibroin (DSF). The DSF was then combined with carboxymethyl chitosan (CMCS) by glutaraldehyde (GA) crosslinking to ensure the structural stability of sponges. The resulting DSF–CMCS–GA exhibited remarkable hemostatic properties, displaying the highest absorption rate. It also demonstrated comparable efficacy to commercial hemostatic sponges. The blood-clotting index and hemolysis test showed that the prepared sponge possessed hemostatic activity and good hemocompatibility. Compared with mulberry silk fibroin hemostatic sponges (SF–CMCS–GA), DSF–CMCS–GA showed slightly better effects, making them a potential alternative to mulberry silk. In conclusion, our study introduces the use of Dictyoploca japonica silk fibroin for hemostasis, highlighting the exploitation of wild silkworm resources and providing an excellent silk fibroin-based hemostatic sealant for acute accident wounds and biomedical applications involving massive hemorrhage.
Pan Cheng,Peng Huang,Cancan Ji,Xiaodan Jia,Qihao Guo,Ming Xia,Qin Cheng,Jia Xu,Ke Liu,Dong Wang
Constructing a sterile membrane with a robust and antifouling surface is a powerful means to improve the sterile filtration efficiency of sterile membranes. In this work, a robust EVOH nanofibrous sterile membrane was facilely fabricated by the method of in situ crosslinking with glutaraldehyde and surface plasma treatment. The resultant EVOH nanofibrous sterile membrane possessed a carboxylated-crosslinked surface, with high hydrophilicity, which generated high chemical stability, high-temperature steam resistance, and an ultrahigh antifouling performance against bovine serum albumin, ribonucleic acid and nanoparticle pollutants. Moreover, the membrane also exhibited a reasonably high primary water permeance (4522.2 LMH bar−1 at 0.2 MPa), as well as an absolute interception rate (100%) of Escherichia coli, Staphylococcus aureus cells and Brevundimonas diminuta superior to the state-of-the-art sterile membrane. Moreover, the modified membrane packed syringe-driven filter presented 100% interception (LRV ≥ 7) to Brevundimonas diminuta and high permeation flux (from 10.8 to 41.8 L·h−1) in a wide operating pressure range of 0.1 MPa to 0.6 MPa, indicating its potential in real bio-separation applications. This work provides a facile strategy for the preparation of a high-performance sterile membrane for biological drug product sterilization.
Graphical Leave a gap ! A carbon dioxide imprinted solid amine adsorbent (IPEIA-R) with polyethylenimine (PEI) as a skeleton was conveniently prepared by using glutaraldehyde to cross-link carbon dioxide-preadsorbed PEI (see figure). The solid amine adsorbent showed a remarkable CO 2 adsorption capacity (8.56 mmol g −1 ) in the presence of water, owing to the high amine content and good swelling properties. A carbon dioxide imprinted solid amine adsorbent (IPEIA-R) with polyethylenimine (PEI) as a skeleton was conveniently prepared by using glutaraldehyde to cross-link carbon dioxide-preadsorbed PEI. As confirmed by FTIR, FT-Raman, and 13 C NMR spectroscopy, CO 2 preadsorbed on PEI could occupy the reactive sites of amino groups and act as a template for imprinting in the cross-linking process. The imino groups formed from the cross-linking reaction between glutaraldehyde and PEI could be reduced by NaBH 4 to form CO 2 -adsorbable amino groups. The adsorption results indicated that CO 2 imprinting and reduction of imino groups by NaBH 4 endowed the adsorbent with a higher CO 2 adsorption capacity. Compared with PEI-supported mesoporous adsorbents, the solid amine adsorbent with PEI as a skeleton can avoid serious pore blockage and CO 2 diffusion resistance, even with a high amine content. The solid amine adsorbent with PEI as a skeleton showed a remarkable CO 2 adsorption capacity (8.56 mmol g −1 ) in the presence of water at 25 °C, owing to the high amine content and good swelling properties. It also showed promising regeneration performance and could maintain almost the same CO 2 adsorption capacity after 15 adsorption–desorption cycles.
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