Frontiers in Physiology
·
2024
·
IF 3.2
Li Xi-Tong,Qian Xiu-Juan,Chen Hong,Wang Xing-Duo,Wu Xia
Glycerophosphocholine (GPC) is a water-soluble small molecule found naturally in humans and foods such as milk and soybeans. It can activate the IIS pathway by regulating the expression of daf-2, ins-18 and daf-16 genes, sek-1 and skn-1 genes of MAPK pathway, sod-3, ctl-1, gst-4 and other antioxidant genes. GPC can relieve symptoms related to aging in organisms. The aim of this study was to probe the effects of GPC on the longevity and stress resistance of the entomopathogenic nematode (EPN) Steinernema kraussei 0657L strain. The results showed that the lifespan of S. kraussei 0657L was significantly prolonged by 50 mM GPC treatment, which was 54.55% longer than that of the control (0 mM GPC). GPC significantly inhibited reactive oxygen species (ROS) and lipofuscin accumulation, but the body size and fecundity of S. kraussei 0657L had little changed. At the same time, the longevity of S. kraussei 0657L exposed to heat shock and UV-B radiation was significantly prolonged than that with no external stress. GPC supplementation increased the activity of antioxidant enzymes and corresponding gene expression. Under treatment with 50 mM GPC, the activities of superoxide dismutase and catalase were increased by 1.90- and 4.13-fold, respectively, the expression of the sod-3 and ctl-1 genes was increased by 3.60- and 0.60-fold, respectively, and harmful reactive oxygen species were removed. In addition, the expression levels of the ins-18, skn-1, sek-1 and gst-4 genes related to the insulin/IGF-1 signaling pathway were upregulated 1.04-, 1.84-, 2.21- and 1.24-fold, respectively. These results indicate that GPC is mainly involved in the lifespan regulation of S. kraussei 0657L and plays an important role in resistance to external stress by activating the insulin/IGF-1 signaling pathway and downstream PI3K/MAPK kinase, creating a new idea for improving the commercial efficacy of S. kraussei. It also laid a theoretical foundation for its further efficient development and utilization in the field of biological control.
研究领域:
分子生物学
·
生物控制
·
遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF CHEMICAL KINETICS
·
2014
·Vol. 46
·期 3
·169-175
·
IF 1.566
Hong-Ya Li,Xiao-Li Zhang,Jie-Bing Zhang,Tian-Tian Zhang,Bin-Xia Zhao
The transesterification of phosphatidylcholine with methanol was successfully carried out using a promising solvent methyl tert-butyl ether instead of traditional ether, and the reaction was dramatically enhanced in the presence of tetrabutylammonium hydroxide as the phase-transfer catalyst. Kinetics of the reaction including the effects of the reaction conditions on the conversion of phosphatidylcholine and the apparent rate constant ( k app ) were investigated in detail. On the basis of the experimental evidence, the transesterification mechanism was proposed and a kinetic model was developed, and the experimental data were well described by the pseudo–first-order equation.
研究领域:
生物化学
·
有机化学
✓ 已验证
DOI
高影响因子
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
·
2022
·Vol. 66
·期 5
·
IF 5.938
Yawei Du,Carlo Giannangelo,Wei He,Gerald J. Shami,Wenya Zhou,Tuo Yang,Darren J. Creek,Con Dogovski,Xinsong Li,Leann Tilley
Current best practice for the treatment of malaria relies on short half-life artemisinins that are failing against emerging Kelch 13 mutant parasite strains. Here, we introduce a liposome-like self-assembly of a dimeric artesunate glycerophosphocholine conjugate (dAPC-S) as an amphiphilic prodrug for the short-lived antimalarial drug, dihydroartemisinin (DHA), with enhanced killing of Kelch 13 mutant artemisinin-resistant parasites. Cryo-electron microscopy (cryoEM) images and the dynamic light scattering (DLS) technique show that dAPC-S typically exhibits a multilamellar liposomal structure with a size distribution similar to that of the liposomes generated using thin-film dispersion (dAPC-L). Liquid chromatography-mass spectrometry (LCMS) was used to monitor the release of DHA. Sustainable release of DHA from dAPC-S and dAPC-L assemblies increased the effective dose and thus efficacy against Kelch 13 mutant artemisinin-resistant parasites in an in vitro assay. To better understand the enhanced killing effect, we investigated processes for deactivation of both the assemblies and DHA, including the roles of serum components and trace levels of iron. Analysis of parasite proteostasis pathways revealed that dAPC assemblies exert their activity via the same mechanism as DHA. We conclude that this easily prepared multilamellar liposome-like dAPC-S with long-acting efficacy shows potential for the treatment of severe and artemisinin-resistant malaria.
研究领域:
药理学
·
寄生虫学
·
生物化学
✓ 已验证
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Hong-Kai Xie,Ao Li,Man-Tong Zhao,Min Zhang,Zhong-Yuan Liu,Fa-Wen Yin,Xiao-Yang Liu,Da-Yong Zhou,Fereidoon Shahidi,Bei-Wei Zhu
Phospholipids (PLs), the main lipid component in marine shellfish, consist mainly of glycerophosphocholine (GPC) and glycerophosphoethanolamine (GPE). This work compared the oxidative susceptibility of GPC and GPE in dried shellfish during storage and also investigated the effects of natural phenolic antioxidant on such oxidative susceptibility. The content of four representative polyunsaturated fatty acids (C18:3 n-3, C20:4 n-6, C20:5 n-3, and C22:6 n-3) esterified onto GPC or GPE in dried scallop adductor muscle during storage at two temperatures were matched with first-order kinetic models, in order to ascertain the oxidation reaction rate constant ( k ). The results showed that GPE possessed greater oxidation rate than GPC during storage, and GPC could be more effectively protected by the antioxidants of bamboo leaves (AOB, polar polyphenolic antioxidants) compared with GPE. This study provides a practical method for accurate evaluation of oxidative susceptibility of different PL classes in complex food systems.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）