COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2018
·
IF 2.829
Alemtsehay Tesfay Reda,Dongxiang Zhang,Xin Lu
A composite material of europium hydroxide (Eu(OH) 3 ) and glycine (Gly) is synthesized successfully to produce single phase material (Eu(OH) 3 -Gly) which exhibits extremely selective capturing and high removal rate towards uranyl (UO 2 2+ ) ion in an aqueous solution. Eu(OH) 3 -Gly displayed distribution coefficient (K d ) of 3.23 × 10 7 mL/g and removal rate of 100% to lower UO 2 2+ ion concentration from 25 ppm to <1 ppb inside 15 min and these criteria place Eu(OH) 3 -Gly among the top existing adsorbents for such adsorption. Adsorption kinetics for UO 2 2+ follows pseudo-second-order model, proposing a chemisorption adsorption mechanism mainly via H 2 N-U-NH 2 and O-U-O interactions. Eu(OH) 3 -Gly showed good stability after the removal of uranyl ion as investigated by FT-IR and thermogravimetric analysis (TGA) methods. According to TG analysis, Eu(OH) 3 -Gly showed less weight loss (23.7%) after adsorption compared with that of before adsorption (32%), suggesting that the attachment of uranium to the adsorbent contributed for the improvement of the thermal stability of Eu(OH) 3 -Gly.
研究领域:
材料科学
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环境科学
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放射化学
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DOI
高影响因子
CRYSTENGCOMM
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2022
·Vol. 24
·期 47
·8321-8325
·
IF 3.756
Shang-Wei Yuan,Pei-Hui Shan,Mao-Xiao Yang,Pei-Hua Ma,Timothy J. Prior,Carl Redshaw,Gang Wei,Zhu Tao,Xin Xiao
Herein, we have investigated the host–guest inclusion complexes formed on interaction of the symmetric tetramethyl-substituted cucurbit[6]uril (TMeQ[6]) with glycine (Gly) under different conditions. The interactions have been studied both in solution (by 1H NMR spectroscopy and mass spectrometry) and in the solid state by single crystal X-ray diffraction. The molecular structures of two products are reported, namely TMeQ[6]·(H2O)2(PO4)(NH3CH2COOH)2 (1) and TMeQ[6]·Ca(H2O)5·(Cl)3(NH3CH2COOH)·16H2O (2). Complex 1 adopts a hydrogen-bonded dimeric structure with two glycinium ions contained within a TMeQ[6] molecule with the NH3+ portions of the two molecules located just above the opposite rims of the bowl and forming hydrogen bonds with C–O. In 2, there are centrosymmetric dimers bridged by pairs of calcium ions.
研究领域:
化学
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材料科学
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DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Chen Li,Zhiwei Liu,Song Liu,Santosh K. Tiwari,Kunyapat Thummavichai,Oluwafunmilola Ola,Zhiyuan Ma,Shenghua Zhang,Nannan Wang,Yanqiu Zhu
In this paper, a multifunctional wound dressing with sustained-release and antibacterial properties was prepared using cellulose acetate (CA) as the matrix and Dimethyloxallyl Glycine (DMOG) and silver nanoparticles (Ag-NPs) as the drug loading component. The scanning electron microscopy shows that the microstructure of the nanofibers is uniform and droplet free, and the diameters of CA and CA/β-CD/DMOG nanofibers are mainly concentrated in the range of 40–120 nm, and the diameters of CA/DMOG/Ag-NPs nanofibers are mainly concentrated in the range of 120–200 nm. In vitro release test confirms that the CA/DMOG/Ag-NPs nanofiber can slowly release DMOG within about 84 h, which abides by a typical diffusion, and the main driving force is the concentration gradient between the drug-carrying nanofibers and the releasing medium. The antibacterial performance test demonstrates that the material exhibits obvious antibacterial performance against both E. coli and B.subtilis bacteria with little adverse effects on cell viability proved by cell compatibility test. Taken together, CA/DMOG/Ag-NPs can be a wound dressing material with good application prospects to promote the healing of diabetic wounds.
研究领域:
生物材料学
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药剂学
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微生物学
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产品关联
DOI
高影响因子
BIOCHEMICAL PHARMACOLOGY
·
2022
·
IF 6.1
Yanbei Tu,Lihua Tan,Tao Lu,Kai Wang,Haiyong Wang,Bing Han,Yuxin Zhao,Hanbin Chen,Yanfang Li,Haixia Chen,Meiwan Chen,Chengwei He
The roots of Glycine tabacina are used to treat rheumatoid arthritis (RA) and joint infection in folk medicine. Glytabastan B (GlyB), a newly reported coumestan isolated from this species, was found to significantly attenuate IL-1β-induced inflammation in SW982 human synovial cells at 3 and 6 μM, as evidenced by the decreased levels of pro-inflammatory mediators and matrix metalloproteinases (MMPs). GlyB also suppressed RANKL-induced osteoclastogenesis, decreased the expression of osteoclastogenic markers (NFATc1, CTSK , MMP-9) and osteoclast-mediated bone resorption. Further, GlyB administration (12.5 and 25 mg/kg) significantly inhibited inflammation, osteoclast formation and disease progression in collagen-induced arthritis (CIA) mice. Integration of network pharmacology , quantitative phosphoproteomic and experimental pharmacology results revealed that these beneficial actions were closely associated with the blockade of GlyB on the activation of MAPK , PI3K/AKT and their downstream signals including NF-κB and GSK3β/NFATc1. Drug affinity responsive target stability (DARTS) assay, cellular thermal shift (CETSA) assay and molecular docking analysis confirmed that there were direct interactions between GlyB and its target proteins ERK2 , JNK1 and class Ⅰ PI3K catalytic subunit p110 (α, β, δ and γ), which significantly contributed to the inhibition of activation of MAPK and PI3K/AKT pathways. In conclusion, these results strongly suggest GlyB is a promising multiple-target candidate for the development of agents for the prevention and treatment of RA.
研究领域:
药理学
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免疫学
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风湿病学
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生物化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MAGNETIC RESONANCE IMAGING
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2025
·Vol. 62
·期 4
·1219-1229
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IF 3.5
Kai Huang,Xiao-Zhu Hao,Cai-Xia Fu,Zhong Yang,Yan Ren,Xue Yang,Shi-Ji Zhang,Mei-Lin Zhu,Zhen-Wei Yao,Da-Xiu Wei,Ye-Feng Yao
Background Glycine (Gly) is a key metabolic intermediate in the proliferation of tumor cells. Monitoring the concentration of Gly in tumor tissues is of great importance for understanding the growth status of tumors. At present, magnetic resonance spectroscopy (MRS) is the only method to non-invasively measure Gly concentration in human tissues. However, in conventional MR spectra the 1 H signal of Gly overlaps with those of other molecules. This makes conventional MRS difficult to accurately measure the Gly concentration in human tissues. Purpose To develop a pulse sequence, Gly-MRS, which can accurately measure Gly concentrations without the influence of the signal overlapping from other molecules in subjects with glioma. Study Type Prospective. Subjects/Phantoms A phantom of the glycine (Gly), myo-inositol (MI) and glutamate (Glu) mixture aqueous solution and 6 phantoms of Gly aqueous solution (pH = 7.2 ± 0.1), 6 subjects with glioma (3 females and 3 males, BMI: 20 ± 4 kg/m 2 , age: 50 ± 10 years). Field Strength/Sequence 3 Tesla/A Gly-targeted magnetic resonance spectroscopy pulse sequence, Gly-MRS, using Point-RESolved Spectroscopy (PRESS) for single voxel signal selection. Assessment By applying the developed pulse sequences to the phantoms and the subjects with glioma, the Gly 1 H signals were successfully selectively probed. Quantification of the signals yields the concentrations of Gly in the regions of the tumor tissues of the subjects with glioma. Statistical Tests Numerical data only. Results The Gly 1 H signals were detected in the tumor regions of 6 subjects with glioma, at a mean concentration of 5.20 mM (standard deviation, ± 3.29 mM). One subject exhibited a clear spatial distribution in the Gly concentrations in the tumor regions. Data Conclusion The Gly-MRS pulse sequence developed in this work might be useful for the accurate in vivo detection of the 1 H signal of Gly in gliomas of human beings. Evidence Level 2. Technical Efficacy Stage 3.
研究领域:
肿瘤学
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神经影像学
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代谢组学
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分子影像
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放射学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENGINEERING IN LIFE SCIENCES
·
2021
·Vol. 22
·期 1
·40-53
·
IF 2.678
Yingying Xu,Jie Ren,Wei Wang,An-Ping Zeng
Glycine cleavage system (GCS) plays a central role in one-carbon (C1) metabolism and receives increasing interest as a core part of the recently proposed reductive glycine pathway (rGlyP) for assimilation of CO 2 and formate. Despite decades of research, GCS has not yet been well understood and kinetic data are barely available. This is to a large degree because of the complexity of GCS, which is composed of four proteins (H, T, P, and L) and catalyzes reactions involving different substrates and cofactors. In vitro kinetics of reconstructed microbial multi-enzyme glycine cleavage/synthase system is desired to better implement rGlyP in microorganisms like Escherichia coli for the use of C1 resources. Here, we examined in vitro several factors that may affect the rate of glycine synthesis via the reverse GCS reaction. We found that the ratio of GCS component proteins has a direct influence on the rate of glycine synthesis, namely higher ratios of P protein and especially H protein to T and L proteins are favorable, and the carboxylation reaction catalyzed by P protein is a key step determining the glycine synthesis rate, whereas increasing the ratio of L protein to other GCS proteins does not have significant effect and the ratio of T protein to other GCS proteins should be kept low. The effect of substrate concentrations on glycine synthesis is quite complex, showing interdependence with the ratios of GCS component proteins. Furthermore, adding the reducing agent dithiothreitol to the reaction mixture not only results in great tolerance to high concentration of formaldehyde, but also increases the rate of glycine synthesis, probably due to its functions in activating P protein and taking up the role of L protein in the non-enzymatic reduction of H ox to H red . Moreover, the presence of some monovalent and divalent metal ions can have either positive or negative effect on the rate of glycine synthesis, depending on their type and their concentration.
研究领域:
生物化学
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代谢工程
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2024
·
IF 5.6
Lin Qiaoxia,Zhang Yonggang,Chen Luhua,Zhang Haoyue,An Chuanfeng,Li Chengze,Wang Qifan,Song Jinhui,He Wei,Wang Huanan
Development of piezoelectric biomaterials with high piezoelectric performance, while possessing excellent flexibility, biocompatibility, and biodegradability still remains a great challenge. Herein, a flexible, biocompatible and biodegradable piezoelectric β-glycine-alginate-glycerol (Gly-Alg-Glycerol) film with excellent in vitro and in vivo sensing performance was developed. Remarkably, a single, monolithic β-glycine spherulite, instead of more commonly observed multiple spherulites, was formed in alginate matrix, thereby resulting in outstanding piezoelectric property, including high piezoelectric constant (7.2 pC/N) and high piezoelectric sensitivity (1.97 mV/kPa). The Gly-Alg-Glycerol film exhibited superior flexibility, enabling complex shape-shifting, e.g. origami pigeon, 40% tensile strain, and repeated bending and folding deformation without fracture. In vitro, the flexible Gly-Alg-Glycerol film sensor could detect subtle pulse signal, sound wave, and recognize shear stress applied from different directions. In addition, we have demonstrated that the Gly-Alg-Glycerol film sensor sealed by polylactic acid and beeswax could serve as an in vivo sensor to monitor physiological pressure signals such as heartbeat, respiration, and muscle movement. Finally, the Gly-Alg-Glycerol film possessed good biocompatibility, supporting the attachment and proliferation of rat mesenchymal stromal cells, and biodegradability, thereby showing great potential as biodegradable piezoelectric biomaterials for biomedical sensing applications.
研究领域:
材料科学
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生物医学工程
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生物材料
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传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL THERMODYNAMICS
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2014
·
IF 2.423
Da-Shun Zhang,Zhi-Cheng Tan,Bei-Ping Liu,Xiao-Bing Huang
Neodymium complex with glycine, Nd(Gly) 2 Cl 3 ·3H 2 O, was synthesized and characterized by IR spectra. The thermal stability of the complex was tested through TG and DTG and a possible mechanism of thermal decomposition was proposed. The heat capacities of the complex were measured by using an automated adiabatic calorimeter over the temperature range from T = (80 to 380) K, the thermodynamic functions, [ H T − H 298.15 ] and [ S T − S 298.15 ], were calculated based on the heat capacity measurements. Two (solid + solid) phase transitions in the ranges of T = (170 to 247) K were observed with the peak temperatures of 184.896 K and 231.217, respectively. The standard molar enthalpy of formation of [Nd(Gly) 2 Cl 3 ·3H 2 O] was determined to be (−3081.3 ± 1.1) kJ · mol −1 in terms of an isoperibol solution-reaction calorimeter.
研究领域:
物理化学
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无机化学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
·
2018
·Vol. 84
·期 13
·
IF 3.633
Ya-Hui Shao,Li-Zhong Guo,Yu-Qing Zhang,Hao Yu,Bai-Suo Zhao,Hai-Qiang Pang,Wei-Dong Lu
Although some bacteria, including Chromohalobacter salexigens DSM 3043, can use glycine betaine (GB) as a sole source of carbon and energy, little information is available about the genes and their encoded proteins involved in the initial step of the GB degradation pathway. In the present study, the results of conserved domain analysis, construction of in-frame deletion mutants, and an in vivo functional complementation assay suggested that the open reading frames Csal_1004 and Csal_1005, designated bmoA and bmoB, respectively, may act as the terminal oxygenase and the ferredoxin reductase genes in a novel Rieske-type oxygenase system to convert GB to dimethylglycine in C. salexigens DSM 3043. To further verify their function, BmoA and BmoB were heterologously overexpressed in Escherichia coli, and 13C nuclear magnetic resonance analysis revealed that dimethylglycine was accumulated in E. coli BL21(DE3) expressing BmoAB or BmoA. In addition, His-tagged BmoA and BmoB were individually purified to electrophoretic homogeneity and estimated to be a homotrimer and a monomer, respectively. In vitro biochemical analysis indicated that BmoB is an NADH-dependent flavin reductase with one noncovalently bound flavin adenine dinucleotide (FAD) as its prosthetic group. In the presence of BmoB, NADH, and flavin, BmoA could aerobically degrade GB to dimethylglycine with the concomitant production of formaldehyde. BmoA exhibited strict substrate specificity for GB, and its demethylation activity was stimulated by Fe2+. Phylogenetic analysis showed that BmoA belongs to group V of the Rieske nonheme iron oxygenase (RO) family, and all the members in this group were able to use quaternary ammonium compounds as substrates.IMPORTANCE GB is widely distributed in nature. In addition to being accumulated intracellularly as a compatible solute to deal with osmotic stress, it can be utilized by many bacteria as a source of carbon and energy. However, very limited knowledge is presently available about the molecular and biochemical mechanisms for the initial step of the aerobic GB degradation pathway in bacteria. Here, we report the molecular and biochemical characterization of a novel two-component Rieske-type monooxygenase system, GB monooxygenase (BMO), which is responsible for oxidative demethylation of GB to dimethylglycine in C. salexigens DSM 3043. The results gained in this study extend our knowledge on the catalytic reaction of microbial GB degradation to dimethylglycine.
研究领域:
微生物学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Meng Xu,Longgang Du,Mengjiao Liu,Jingjie Zhou,Wankun Pan,Haoran Fu,Xin Zhang,Qingxu Ma,Lianghuan Wu
To determine whether high spraying concentrations of Zn sources increase the Zn concentration in waxy corn ( Zea mays L. var. ceratina Kulesh) seeds without compromising agronomic performance, field experiments were conducted between 2018 and 2020. Excess ZnSO 4 application caused foliar burn, barren ear tip, and grain yield loss. ZnEDTA and Glycine-chelated Zn (ZnGly) caused less foliar burn, but Glycine-mixed Zn caused more foliar burn than ZnSO 4 . The seed Zn concentration increased with spraying Zn concentration. ZnEDTA (≤0.8%) had a higher threshold concentration than ZnGly (≤0.4%). Nevertheless, Zn biofortification efficacy did not significantly differ between 0.4% ZnGly and 0.8% ZnEDTA, and the grain Zn recovery rate of 0.4% ZnGly was much higher than that of 0.8% ZnEDTA. Additionally, dual-isotope labelling tests confirmed that 15 N-glycine and 68 Zn in ZnGly interacted. In the future, chelating technology is essential for developing new Zn fertilizers to optimize Zn biofortification efficacy.
研究领域:
农业
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植物科学
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营养管理
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作物生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2018
·
IF 3.235
Wenbin Zhang,Peng Chen,Shengyou Chen,Yuyang Wei
In order to emphasize the importance of electrolyte-tuning in the nitroxyl radicals mediated electrocatalytic reactions , methanol and glycine electrooxidation reactions catalyzed by TEMPO under different properties (pH, identity, concentration) of buffer solutions were investigated by cyclic voltammetry . It was discovered that in both carbonate buffer solutions and borate buffer solutions, catalytic abilities increase with the rising of pH for both glycine electrooxidation and methanol electrooxidation. The electrocatalytic performance is also affected by the identity of buffer, for both cases of glycine and methanol, the electrooxidation activities in borate buffer solutions are apparently higher than those in carbonate buffer solutions. For both glycine and methanol electrooxidation, relationships between concentrations of carbonate ions and relative catalytic current densities were observed to be volcano-shaped, while electrocatalytic abilities increase with the increasing of concentrations of borate ions within the studied concentration range. These findings suggest that the electrolyte should be treated as important as the catalyst in the designing of nitroxyl radicals mediated electrocatalytic systems.
研究领域:
电化学
·
催化
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Xiongxiong Zhang,Mei Yang,Yonggang Sun,Yuanyuan Li,Yulong Ma,Wenxin Ji
The indirect mineralization of CO 2 by industrial solid waste is an essential process for CO 2 immobilization; however, many technical challenges remain to be overcome. In this study, carbide slag (CS), a type of industrial solid waste, was used as a raw material for the indirect mineralization of CO 2 using glycine. In the extraction stage, the ion extraction efficiency of Ca significantly increased to 68.5 % due to the formation of a chelate between glycine and Ca, which enhanced Ca dissolution. Analysis of the mineralization process revealed that glycine was recycled after absorbing CO 2 , leading to increased CO 2 absorption capacity because of the high alkalinity of the extract solution. Furthermore, there was a synergistic effect between CO 2 absorption and the Ca precipitation reaction. This synergistic effect increased the reaction rate of mineralization and enhanced its efficiency. As a result, the Ca precipitation reaction was largely complete within 5 min, and the mineralization efficiency was enhanced to 93 %. Furthermore, the recirculation (indirect mineralization) results showed that the Ca extraction and mineralization efficiencies remained constant during each cycle. Most importantly, glycine could be regenerated automatically during the cycle, which proved that the cyclic indirect mineralization process could operate stably.
研究领域:
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）