In a minimally invasive procedure, hydrogels can be injected into an affected area for drug loading and tissue repair. Here, gelatin methacryloyl (GelMA) was modified with alendronate (ALN), and three different alendronate-functionalized GelMA (GelMA-ALN) hydrogels were prepared. The modification greatly improved the swelling ratio, protein adsorption and mineralization. The GelMA-ALN hydrogels significantly promoted the in vitro osteogenic differentiation of hFOB cells as indicated by their higher alkaline phosphatase (ALP) activity, denser mineralization and up-regulated osteogenesis-related genes at both the mRNA and protein levels. Meanwhile, the cells maintained their activity and differentiated into osteoblasts when encapsulated in the GelMA-ALN hydrogels. Alendronate-modified hydrogels have potential for use in the minimally invasive treatment of irregular bone defects.
Lei Liu,Yingcong He,Xuetao Shi,Huichang Gao,Yingjun Wang,Zhengmei Lin
By mimicking the role of phosphate groups in native tissues and encouraging tissue formation, phosphate-functionalized biopolymers have been widely employed to accelerate the formation of bone injury. In this study, phosphocreatine is screened as a phosphate source and applied in the preparation of phosphocreatine-modified chitosan (CS-P) and porous scaffolds. CS-P exhibits much better hydrophilicity, solubility, protein adsorption and mineralization than pure chitosan (CS). Meanwhile, in comparison to CS scaffold, the CS-P scaffold significantly enhances the in vitro osteogenic differentiation of hFOB cells, indicated by the higher alkaline phosphatase (ALP) activity, denser mineralization deposition and up-regulated osteogenesis-related genes including runt-related transcription factor-2, ALP, osteocalcin and osteopontin at both mRNA and protein levels. The in vivo results indicate an enhancing effect of the CS-HP scaffold on the bone regeneration ability. We further confirm that phosphocreatine modification promotes the osteogenic differentiation of hFOB cells by activating the MAPK signalling pathway. The phosphocreatine-modified chitosan holds great potential for bone repair and regeneration.
危险品化学品经营许可证（带存储）