NEW JOURNAL OF CHEMISTRY
·
2014
·Vol. 38
·期 8
·3673-3677
·
IF 3.159
Xi Juan Zhao,Cheng Zhi Huang
A one step synthesis of water-soluble luminescent copper nanoclusters (CuNCs) reduced and protected by histidine is reported. When excited at 350 nm, the as-synthesized CuNCs exhibit luminescence centered at 456 nm with a quantum yield of 1.6%, which is different from that of histidine alone under the same experimental conditions. Upon addition of guanosine 5′-triphosphate (GTP), the emission of CuNCs is gradually quenched. While other nucleoside triphosphates including ATP, CTP and UTP as well as some inorganic anions such as P2O74−, PO43−, and CH3COO− have no such quenching effect, which indicates that the nucleobase instead of the phosphate anion plays an important role in the emission quenching of CuNCs. The most efficient quenching of the CuNCs induced by GTP can be ascribed to the most reducing ability of guanine among the other nucleobases. This interesting experimental result demonstrates that the as-synthesized CuNCs can be applied to sensing of GTP.
研究领域:
纳米材料
·
分析化学
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生物传感器
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纳米技术
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DOI
高影响因子
Water
·
2024
·Vol. 16
·期 16
·2227
·
IF 3
Hao Peng,Laixin Wang,Wenjing Pan,Sisi Yang,Jingjing Wang,Jielin Qin,Lihua Ao,Yinhe Lin,Jinzhu Tang
In this paper, histidine was applied to treat vanadium-containing wastewater. Several independent experimental parameters, including H2SO4concentration, dosage of histidine, reaction time and reaction temperature, were investigated and optimized through response surface methodology. The influence on the reduction process decreased in the following order: dosage of histidine > reaction temperature > reaction time. The reduction efficiency could be achieved at 95.77% under the following reaction conditions: H2SO4concentration of 0.2 mol/L, reaction temperature of 90 °C, dosage of histidine at n(His)/n(V) = 3.6, reaction time of 60 min and stirring rate at 500 rpm. The reduction kinetics was followed successfully with the pseudo-first-order kinetics model and theEafor reduction of vanadium was calculated to be 25.31 kJ/mol. The reduction kinetics was affected by these factors and the kinetics model could be described by an Equation. This paper provides a versatile strategy for treatment of wastewater containing V(V) and shows a bright tomorrow for wastewater treatment.
研究领域:
环境科学
·
生物化学
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产品关联
DOI
高影响因子
ACS Nano
·
2022
·
IF 18.027
Xiaoya Ding,Yunru Yu,Luoran Shang,Yuanjin Zhao
Graphene oxide (GO) hydrogels have provided tremendous opportunities in designing and fabricating complex constructs for diverse applications, while their 3D printing without photocuring is still a challenging task due to their low viscosity, uncontrollable gelation, and low interfacial tension. Here, we report a histidine-assisted printing strategy to prepare GO hybrid hydrogels through the microfluidic 3D printing technique. We found that the GO additive could significantly hamper the Knoevenagel condensation (KC) reaction between benzaldehyde and cyanoacetate group-functionalized polymers to form a hydrogel, while these GO mixed solutions were rapidly solidified into a hydrogel when histidine was added. This fascinating phenomenon enabled us to prepare low-viscosity GO mixed polymer solutions as printable inks and generate hydrogel microfibers in histidine solutions. The hydrogel fibers could support cell survival and be further constructed into complex 3D structures through microfluidic 3D printing techniques. Moreover, due to the addition of GO, the microfibers exhibited excellent electrical conductivity and could sense the motion changes and convert these stimuli as electrical resistance signals. This strategy adds an option for the design and application of 3D printable aqueous GO inks in many fields.
研究领域:
材料科学
·
生物医学工程
·
化学工程
✓ 已验证
DOI
PubMed
高影响因子
CARBOHYDRATE RESEARCH
·
2023
·
IF 3.1
Kuan Li,Jinling Wang,Yadong Zhuang,Guangxiang Yuan,Yuxiu Li,Xiaolan Zhu
N-(2-Deoxy- D -glucos-2-yl)- L -histidine (Glu-His), one of Heyns rearrangement products (HRPs), was prepared by condensation , dehydration and rearrangement using l -Histidine and d -Fructose as raw materials with methanol as solvent. The response surface method (RSM) was used to improve yield of product and the optimal reaction condition was as following: the original ratio of Fru:His was 1.2:1 and the temperature and time of reaction was 73.2 °C and 4.7 h, and the yield of final product was 74.10% with the purity of 99.7%. The structure of product was identified by IR, NMR and conformed as C 12 H 19 N 3 O 7 (317.1 Da) by high-resolution mass spectrometry (HRMS) and UPLC-MS/MS. The pyrolysis behavior of Glu-His showed that its initial pyrolysis temperature was 145.2 °C and the total weight loss reached 70.61% at 800 °C. The number of pyrolysis products increased with the increase of temperature, and the main pyrolysis products were pyrans , furans, pyrazines , pyrroles , pyridines, indoles and etc. with burnt-sweet, baking, nutty, sweet and floral aroma features. At last, the fragrance enhancement effect of Glu-His in the preparation of reconstructed tobacco stem (RTS) was investigated and the result of sensory evaluation showed that the smoke of RTS cigarettes brought about more sweet and moist, less irritation, better flavor and comfort with the addition of Glu-His (0.25%, w/w).
研究领域:
食品科学
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化学
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产品关联
DOI
PubMed
高影响因子
DALTON TRANSACTIONS
·
2015
·Vol. 44
·期 42
·18671-18676
·
IF 4.197
Quankun Gao,Bo Song,Zhiqiang Ye,Liu Yang,Ruoyang Liu,Jingli Yuan
In this work, we designed and synthesized a heterobimetallic ruthenium(II)–nickel(II) complex, [Ru(bpy)2(phen-DPA)Ni](PF6)4 (Ru–Ni), as a highly selective phosphorescence probe for histidine. The probe exhibited weak emission at 603 nm because the phosphorescence of the Ru(II) complex can be strongly quenched by the paramagnetic Ni2+ ion. In the presence of histidine, reaction of Ru–Ni with histidine resulted in the release of nickel(II) and an enhancement in the phosphorescence intensity at 603 nm. Ru–Ni showed high selectivity for histidine even in the presence of other amino acids and cellular abundant species. Cell imaging experimental results demonstrated that Ru–Ni is membrane permeable, and can be applied for visualizing histidine in live cells. More interestingly, Ru–Ni also can act as a novel reaction-based nuclear staining agent for visualizing exclusively the nuclei of living cells with a significant phosphorescence enhancement. In addition, the potential of the probe for biological applications was confirmed by employing it for phosphorescence imaging of histidine in larval zebrafish and Daphnia magna. These results demonstrated that Ru–Ni would be a useful tool for physiological and pathological studies involving histidine.
研究领域:
生物化学
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分析化学
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Jia Kong,Shixuan Zhao,Xue Han,Wenxin Li,Jiaxing Zhang,Yuefei Wang,Xihui Shen,Yinqiang Xia,Zhonghong Li
Fluorescent proteins (FPs) provide a ratiometric readout for quantitative assessment of the destination of internalized biomolecules. FP-inspired peptide nanostructures that can compete with FPs in their capacity are the most preferred building blocks for the synthesis of fluorescent soft matter. However, realizing a ratiometric emission from a single peptide fluorophore remains exclusive since multicolor emission is a rare property in peptide nanostructures. Here, we describe a bioinspired peptidyl platform for ratiometric intracellular quantitation by employing a single ferrocene-modified histidine dipeptide. The intensiometric ratio of green to blue fluorescence correlates linearly with the concentration of the peptide by three orders of magnitude. The ratiometric fluorescence of the peptide is an assembly-induced emission originating from hydrogen bonds and aromatic interactions. Additionally, modular design enables ferrocene-modified histidine dipeptides to use as a general platform for the construction of intricate peptides that retain the ratiometric fluorescent properties. The ratiometric peptide technique promises flexibility in the design of a wide spectrum of stoichiometric biosensors for quantitatively understanding the trafficking and subcellular fate of biomolecules.
研究领域:
生物化学
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纳米技术
·
分子生物学
·
生物物理学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Shiyi Li,Lei Li,Mengran Ma,Meining Xing,Xiaohong Qian,Wantao Ying
Background Histidine phosphorylation (pHis) plays a key role in signal transduction in prokaryotes and regulates tumour initiation and progression in mammals. However, the pHis substrates and their functions are rarely known due to the lack of effective analytical strategies. Results Herein, we provide a strategy for unbiased enrichment and assignment of the pHis peptides. First, the entire procedure was designed under alkaline conditions to maintain the stability of the N–P bond of pHis and high-pH reverse-phase chromatography was used to efficiently separate the pHis peptides. Second, exploiting the coelution benefits of diethyl labelling, the ratios of light- and heavy-labelled peptides were accurately quantified, and the sites of phosphorylated histidine were assigned. Finally, Cu-IDA bead enrichment and data-independent acquisition mass spectrometry analysis were used to improve the coverage of the histidine phosphoproteome. With this novel strategy, 768 and 1125 potential pHis peptides were identified from lysates of E. coli and HeLa cells, respectively. And these values represent the highest coverage of the histidine phosphoproteome for both cell types. Significance These data strongly support the presumption that pHis modifications are widely present in bacteria. The study provides an efficient strategy and can lead to a better understanding of pHis-modified substrates and their biological functions.
研究领域:
生物化学
·
分子生物学
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蛋白质组学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2013
·Vol. 1
·期 38
·5108-5113
·
IF 0
Xueyan Zou,Kun Li,Yanbao Zhao,Yu Zhang,Binjie Li,Chunpeng Song
Ferroferric oxide/L-cysteine (Fe3O4/Cys) nanospheres (NSs) have been successfully synthesized via a facile solvothermal route. Fe3O4/Cys NSs possessed high thiol group density and saturation magnetization (Ms) of 84.6 emu g−1. The prepared magnetic NSs are biocompatible and manipulatable by an external magnetic force. After chelating Ni2+ ions, Fe3O4/Cys-Ni2+ NSs were used to enrich and purify histidine-tagged (His-tagged) proteins directly from the mixture of lysed cells without pretreatment. It has been found that Fe3O4/Cys-Ni2+ NSs present negligible nonspecific protein adsorption and high protein binding activity with the saturation capacity being 53.2 μg mg−1 and they are especially suitable for rapid purification of His-tagged proteins.
研究领域:
生物化学
·
纳米医学
·
蛋白质工程
✓ 已验证
产品关联
DOI
PubMed
Materials Science & Engineering C-Materials for Biological Applications
·
2014
·
IF 2.736
Shasha Yao,Yanqin Huang,Yanbao Zhao,Yu Zhang,Xueyan Zou,Chunpeng Song
A simple strategy has been developed to synthesize hydroxyapatite (HAP) nanoparticles (NPs) in a simulated body fluid (SBF). The HAP NPs have an average diameter of 50 nm and present porous structure. By taking advantage of surface hydroxyl groups, the HAP NPs are further modified with iminodiacetic acid (IDA), followed by chelating Ni 2 + ions. The HAP/IDA-Ni 2 + NPs as novel adsorbent can capture directly histidine-tagged (His-tagged) proteins from the mixture of lysed cells without sample pretreatment. Results indicated that the HAP/IDA-Ni 2 + NPs present negligible nonspecific adsorption and high protein binding ability, and their specificity and affinity toward His-tagged proteins can remain after 5 times of recycling. The HAP/IDA-Ni 2 + NPs are especially suitable for purification of His-tagged proteins with low molecule weight.
研究领域:
材料科学
·
生物化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2020
·
IF 4.049
Shuyue Wang,Hongqiang Qin,Jing Dong,Lianghai Hu,Mingliang Ye
Sialylation, an important form of glycosylation, is involved in many biological processes and plays an important role in the development of diseases. However, due to the low abundance among various glycosylation and lack of efficient enrichment method with high specificity, the study of sialylation remains a challenge. Herein, multi-histidine modified microspheres (MHM) were synthesized to enrich sialylated glycopeptides. It was found that MHM could selectively enrich sialylated glycopeptides from over 100 times of non-sialylated glycopeptides, which indicated MHM possessed good enrichment specificity towards sialylated glycopeptides. Furthermore, MHM were utilized to the large-scale analysis of protein sialylation, and 510 intact glycopeptides were identified with over 94.5% sialylated glycopeptide specificity from 4 μL human serum. The good specificity could be attributed to the synergistic effect by the electrostatic interaction and hydrophilic interaction. Hence, MHM could provide an alternative approach for the analysis of site-specific sialylation at proteome level from complex biological samples.
研究领域:
蛋白质组学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2021
·Vol. 13
·期 17
·8174-8180
·
IF 7.79
Zehao Gu,Mengfan Lu,Kai Feng,Zhaoxia Jin
Cellulose nanocrystals (CNCs) are inherently right-handed nanostructures that originate from nature, showing chirality in their fibrils, bundles, and self-assembled films. However, the enantio-specific interaction between CNCs and other chiral molecules has not been explored so far. In this study, we first demonstrated a chirality-related difference in the composite films of cellulose nanocrystals and histidine with a D- or L-configuration. The distinction is not only presented in the self-assembled nanostructures of CNCs, optical properties, and the thermal decomposition of composites but also in the crystallization of the amino acid. We suppose that it might have originated from the packing of amino acids on the twisted surface of CNCs. The knowledge about the enantio-specific interaction between the chiral amino acid and polysaccharide nanostructure is of significant importance for developing a new strategy for enantiomeric separation.
研究领域:
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2023
·
IF 6.1
Qibo Yan,Ming Li,Yanxin Zhang,Hailong Liu,Feng Liu,Weiwei Liao,Yingwu Wang,Haifeng Duan,Zhonglin Wei
Chemical cross-linking mass spectrometry (XL-MS) significantly contributes to the analysis of protein structures and the elucidation of protein-protein interactions. Currently available cross-linkers mainly target N-terminus, lysine, glutamate, aspartate, and cysteine residues in protein. Herein, a bifunctional cross-linker, named [4,4'-( d isulfanediylbis(ethane-2,1-diyl)) b is(1- m ethyl-1,2,4- t riazolidine-3,5-dione)] (DBMT) has been designed and characterized aiming to extremely expand the application of XL-MS approach. DBMT is capable of selectively targeting tyrosine residue in protein via an electrochemical click reaction, and/or targeting histidine residue in protein in the presence of 1 O 2 generated under photocatalytic reaction. A novel cross-linking strategy based on this cross-linker has been developed and demonstrated using model proteins, which provides a complementary XL-MS tool analyzing protein structure, protein complexes, protein-protein interactions, and even protein dynamics.
研究领域:
生物化学
·
结构生物学
·
蛋白质组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）