TALANTA
·
2018
·
IF 4.244
Lizhen Liu,Zhi Mi,Qin Hu,Caiqing Li,Xiaohua Li,Feng Feng
In this article, we presented a new rapid, sensitive and selective method for the determination of hyperin (Hyp) based on the fluorescence quenching of fluorescent carbon dots (CDs). The CDs were prepared by simply mixing an aqueous solution of citric acid with diphosphorus pentoxide. This one-step synthetic route is fast and simple with neither high temperature nor complicated synthesis steps is involved. When Hyp was added to CDs solution, the fluorescence intensity of the CDs significantly decreased. The CDs display high selectivity for Hyp over many potentially interfering substances. Under the optimized conditions, a good linear relationship between the fluorescence intensity ratio F o / F and the concentration of Hyp is obtained in a range of 0.22–55 µM with a detection limit ( S / N = 3) of 78.3 nM. The method was successfully applied for the determination of Hyp in fufangmuji granules and human serum samples with recoveries in a range of 93.3–107.0%. This paper highlights the usefulness of CDs as an effective fluorescence probe for the Hyp detection due to its easy preparation, low-cost, excellent photostability, favorable biocompatibility and low cytotoxicity.
研究领域:
分析化学
·
药物分析
·
生物传感
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产品关联
DOI
PubMed
高影响因子
NANO
·
2019
·
IF 1.293
Na Wang,Jianyao Kou,Lijie Wang,Fei Wang,Kui Lu
A novel electrochemically activated ZrO 2 nanoparticles modified carbon paste electrode (CPE) (ZrO 2 -CPE(ox)) was prepared and applied for selective and sensitive determination of hyperin. Scanning electron microscopy (SEM), X-ray diffraction (XRD), Raman spectra and electrochemical experiment were used to characterize the ZrO 2 nanoparticles and investigate the electrochemical performance of the ZrO 2 -CPE(ox). Compared with bare CPE and electrochemically activated CPE (CPE(ox)), the ZrO 2 -CPE(ox) got more reactive sites by electrochemical treatment and exhibited larger effective surface area and more excellent electrochemical catalytic activity toward the redox of hyperin. Under the optimum conditions, the oxidation peak currents showed a linear relationship with the concentrations of hyperin in the range of 5 . 0 × 1 0 − 9 – 2 . 0 × 1 0 − 6 mol L − 1 and 2 . 0 × 1 0 − 6 – 1 . 2 × 1 0 − 5 mol L − 1 , and a low detection limit of 1 . 0 × 1 0 − 9 mol L − 1 was achieved for hyperin. Moreover, the proposed method exhibited an excellent selectivity, stability, reproducibility and repeatability, which make it very suitable for a potential choice for determining the concentrations of hyperin in drug matrices.
研究领域:
分析化学
·
药物分析
·
电化学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Yunting Mao,Liyue Hou,Ligai Bai
In this work, a monolithic cartridge packed with lignin polymer (LiP)-dopped adsorbent was fabricated in a stainless steel tube via polymerization using divinyltetramethyldisilane as the monomer, which aimed for the extraction and separation of hyperin from Senecionis Scandentis Hebra (SSH). The fabricated monolithic cartridge used for the solid-phase extraction (SPE) method was characterized by scanning electron microscope, fourier transform infrared spectrometer, specific surface area measuring instrument and mercury intrusion porosimetry, which confirms a macro- and meso -porous structure with high specific surface area of 333.2 m 2 /g of the optimized adsorbent. An SPE-HPLC method was established by combining the prepared monolithic cartridge and a C18 analytical column via HPLC system for the online extraction, enrichment and quantitative determination of hyperin from SSH. The results indicate that the prepared monolithic cartridge shows not only specific selectivity for the target component of hyperin, but also outstanding removal ability for the sample matrix. Methodology validation was carried out to verify the proposed quantitative analytical method, including the regression equation and related data, the precision, the spiked recovery and the preparative repeatability of the SPE cartridge, which results confirm the feasibility of the present method for the extraction and quantitative determination of hyperin from SSH.
研究领域:
化学工程
·
药学
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产品关联
DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Lijie Wang,Wenke Jia,Yanju Wu,Fei Wang,Lu Kui
In this work, pulse potential method (PPM) was proposed to fabricate directly three dimensional graphene (3DG)-multi walled carbon nanotubes (MWCNTs) (3DG-MWCNTs) network on the surface of glassy carbon (GC) electrode in the graphene oxide (GO)-multi walled carbon nanotubes (MWCNTs) (GO-MWCNTs) suspension. GO-MWCNTs and 3DG-MWCNTs were characterized by using scanning electron microscopy (SEM), UV-visible spectrophotometry (UV-vis) and electrochemical experiments. The 3DG-MWCNTs network as a voltammetric material was presented in developing a novel electrochemical sensor for determination of hyperin. Systematic electrochemical tests confirmed that the 3DG-MWCNTs network modified electrode can effectively increase the response to the redox of hyperin, compared with that of bare GC electrode and 3DG network modified electrode. Under the optimal conditions, the response of the sensor demonstrated a linear relationship with hyperin concentration in the range of 5.0 × 10 -9 ~ 1.5 × 10 -6 mol L -1 with a low detection limit of 1.0 × 10 -9 mol L -1 . Moreover, the proposed method showed excellent performance in stability, sensitivity, selectivity and reproducibility for the determination of hyperin.
研究领域:
材料科学
·
分析化学
·
生物医学工程
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DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2017
·
IF 4.811
Dong-Yang Zhang,Ning Zhang,Peng Song,Jing-Yi Hao,Yi Wan,Xiao-Hui Yao,Tao Chen,Long Li
In the present study, porous magnetic cellulose beads (CBs) were prepared and further modified using amines. The CBs appeared to have good spherical shape and three-dimensional (3D) porous structure. In the adsorption tests, the modified cellulose beads (MCBs) showed better adsorption capacities and shorter adsorption times on hyperin and 2′-O-galloylhyperin than the commercial resins. The adsorption may be due to the hydrogen bonding between the target compounds and the amine groups of MCBs. After adsorption and desorption, the contents of hyperin and 2′-O-galloylhyperin reached 1.32% and 3.92%, which were 4.08 and 4.23 times higher than those in the Pyrola extracts. Therefore, the prepared MCBs in this study make an excellent adsorbing material of hyperin and 2′-O-galloylhyperin, and it may have potential for the separation of other natural compounds.
研究领域:
材料科学
·
天然产物化学
·
药用植物研究
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2012
·Vol. 137
·期 17
·4076-4085
·
IF 4.23
Xiao Lin,Yuzhi Wang,Xiaojie Liu,Songyun Huang,Qun Zeng
Ionic liquids-based microwave-assisted extraction (ILs-MAE) of medicinal or useful compounds from plants was investigated as an alternative to conventional organic solvent extractions. The extraction and the preconcentration of aqueous two-phase (ATP) systems have been integrated. Various operating parameters were systematically considered by single-factor and L9 (34) orthogonal array experiments. 1-Butyl-3-methylimidazolium tetrafluoroborate ([bmim][BF4]) has been selected to extract Apocynum venetum. The extract was then converted to the top phase by [bmim][BF4]/NaH2PO4 system which was suitable for the preconcentration. Reversed phase high performance liquid chromatography (RP-HPLC) with ultraviolet detection was employed for the analysis of hyperin and isoquercitrin in Apocynum venetum. The optimal experiment approach could provide higher detection limit of hyperin and isoquercitrin which were 3.82 μg L−1 and 3.00 μg L−1 in Apocynum venetum. The recoveries of hyperin and isoquercitrin were 97.29% (RSD = 1.02%) and 99.40% (RSD = 1.13%), respectively, from aqueous samples of Apocynum venetum by the proposed method. Moreover, the extraction mechanism of ILs-MAE and the microstructures and chemical structures of the herb before and after extraction were also investigated. The method exhibited potential applicability with other complicated samples.
研究领域:
药学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2016
·
IF 2.822
Huichao Li,Kai Sheng,Zhengkun Xie,Lina Zou,Baoxian Ye
A simple electrochemical method for the highly sensitive determination of hyperin was established based on poly(diallyldimethylammonium chloride)-functionalized graphene modified electrode (PDDA-Gr/GCE). The nanocomposite was characterized by X-ray diffraction (XRD), ultraviolet/visible spectra (UV–vis), Fourier Transform infrared spectroscopy (FT-IR) and transmission electron microscopy (TEM). The electrochemical characteristics of hyperin were studied in detail via cyclic voltammetry (CV), chronocoulometry (CC). Dynamics parameters of electrode process were also calculated to discuss the reaction mechanism. Under the optimized conditions, a lower detection limit of 5 × 10 − 9 mol L − 1 (S/N = 3) and a wide linear detection range from 7 × 10 − 9 to 7 × 10 − 7 mol L − 1 were achieved by the differential pulse voltammetry (DPV). Additionally, this simple, sensitive sensor was proved to be suitable for the determination of hyperin in Chinese herb Hypericum Perforatum with satisfactory results.
研究领域:
分析化学
·
药学
✓ 已验证
产品关联
DOI
高影响因子
Evidence-based Complementary and Alternative Medicine
·
2021
·
IF 2.63
Fang You,Junyan Cao,Li Cheng,Xiaogu Liu,Li Zeng
Premature ovarian insufficiency (POI) is characterized by the loss of ovarian function before 40 years of age and affects approximately 1% of women worldwide. Caragana sinica is a traditional Miao (a Chinese ethnic minority) medicine that improves ovarian function and follicular development. In the present study, we aimed to investigate the effect of active ingredients of C. sinica on POI and determine underlying mechanisms. Herein, the chemical composition of the C. sinica compound was analyzed using ultra-high-performance liquid chromatography, which identified hyperin (HR) as one of the main ingredients in C. sinica. Then, interaction targets of HR and POI were predicted and analyzed using network pharmacology and bioinformatics. The effect of HR on triptolide (TP)-induced granulosa cell injury was evaluated, and the underlying mechanism was explored based on bioinformatic results. A total of 100 interaction targets for POI and HR were obtained. The protein-protein interaction network of identified interaction targets emphasized the topological importance of AKT1. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that HR might regulate POI by modulating the mechanistic target of rapamycin (mTOR) signaling pathway. In addition, the KEGG graph of the mTOR signaling pathway revealed that AKT phosphorylation inhibits the TSC1/2, while TSC1/2 activation inhibits the expression of mTORC1. The fundamental experiment revealed that HR increased proliferation, progesterone receptor levels, and estradiol levels decreased by TP in KGN cells. Additionally, HR alleviated TP-induced apoptosis and G1/G1 phase arrest in KGN cells. Western blotting demonstrated that HR increased the phosphorylation of AKT and mTORC1 and decreased TSC1 expression in TP-induced KGN cells. Collectively, our findings revealed that HR alleviates TP-induced granulosa cell injury by regulating AKT/TSC1/mTORC1 signaling, providing insight into the treatment of POI.
研究领域:
药理学
·
细胞生物学
·
生殖医学
·
传统中药
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Liangmei Rao,Yifu Zhu,Zhongshu Duan,Ting Xue,Xuemin Duan,Yangping Wen,Annamalai Senthil Kumar,Weiming Zhang,Jingkun Xu,Akbar Hojjati-Najafabadi
Biomass waste, a good candidate for advanced carbon materials for sustainable electrodes, is receiving more and more attention for high value-added materials because of its promising contribution to economic growth and sustainable development. We proposed a green co-hydrothermal approach to prepare lotus seedpods biochar (BC) decorated molybdenum disulfide (MoS 2 ) from waste lotus seedpods and precursors of MoS 2, and a portable, flexible, outdoor and inexpensive sensing platform for hyperin on the integrated flexible three-electrode using U-disk potentiostat with smartphone was successfully developed. Structure and properties of MoS 2 -BC were characterized, it was proved that BC improves microstructure and morphology, electronic conductivity, electrode stability and electrocatalytic properties of MoS 2 . Attributing to these impressive features, the detection signal of hyperin was significantly amplified by the MoS 2 -BC modified glass carbon electrode (GCE) in detection range of 0.01–21 μΜ with detection limit (LOD) of 5 nM. It was worth mentioning that the MoS 2 -BC modified screen-printed electrode (SPE) performs hyperin detection in range of 100 nM - 3 μM with LOD 50 nM (S/N = 3). The practicability of the proposed method confirmed that the portable, on-site, low-cost, and outdoor detection of hyperin was feasible and practical in comparison with traditional both electrochemical sensing and HPLC methods.
研究领域:
材料科学
·
环境科学
·
电化学
·
可持续发展
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Shuo Li,Sheng Lei,Qian Yu,Lina Zou,Baoxian Ye
A novel high-sensitive electrochemical sensor with glassy carbon electrode (GCE) as support for hyperin determination is successfully designed and constructed, and the well-shaped nano-meter modified material is synthesized via a one-step and facile route. Functionalized with surfactant sodium dodecyl sulfate (SDS), Single-Walled Carbon Nanotubes (SWCNTs) are synchronously grafted with ZrO 2 nanoparticles to develop into the as-prepared nano-composite (ZrO 2 -SDS-SWCNTs). Compared to the previous reports related with hyperin detection, the linear range gets wider and detection limit (LOD) becomes lower with the aid of this novel nano-composite modified glassy carbon electrode (ZrO 2 -SDS-SWCNTs/GCE). The crystalline phases and functionalization of the preparation process has been investigated by X-ray diffraction (XRD) and Fourier transform infrared (FT-IR) instrument analysis, respectively, and the micro-morphology of related modified materials is also visibly characterized by scanning electron microscope (SEM) and transmission electron microscope (TEM). In addition, electrochemical properties of the modified materials are comparably explored by means of impedance spectroscopy (EIS) and cyclic voltammograms (CV). According to the established calibration curve under optimized condition, the peak current (Differential pulse voltammetry (DPV) signal) keeps a linear relationship with hyperin concentration in the ranges of 1.0 × 10 −9 − 3.0 × 10 −7 mol L −1 , meanwhile detection limit reaches as low as 5 × 10 −10 mol L −1 (S/N = 3). As for practical applications, the proposed sensor has also worked well on sensitive hyperin determination in real species Abelmoschus manihot.
研究领域:
分析化学
·
纳米技术
·
电化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2016
·
IF 2.653
Xiaojun Zou,Fang Shang,Sui Wang
In this paper, a novel electrochemiluminescence (ECL) sensor of sol-gel@graphene luminescent composite film modified electrode for hyperin determination was prepared using graphene (G) as solid-phase microextraction (SPME) material, based on selective preconcentration of target onto an electrode and followed by luminol ECL detection. Hyperin was firstly extracted from aqueous solution through the modified GCE. Hydrogel, electrogenerated chemiluminescence reagents, pH of working solution, extraction time and temperature and scan rate were discussed. Under the optimum conditions, the change of ECL intensity was in proportion to the concentration of hyperin in the range of 0.02–0.24 μg/mL with a detection limit of 0.01 μg/mL. This method showed good performance in stability, reproducibility and precision for the determination of hyperin.
研究领域:
分析化学
·
药物分析
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）