TALANTA
·
2023
·
IF 6.1
Yilin Peng,Yaqi Jin,Di Sun,Zihui Jin,Qiyang Zhao,Yue He,Bining Jiao,Yongliang Cui,Yaohai Zhang
Fenpyroximate is a systemic phenoxy pyrazole acaricide applied worldwide to prevent and control various phytophagous mites and has high activity against young mites, with residues increasingly being found in ecological environment and agricultural products. To identify its residues, four haptens of fenpyroximate were designed and an indirect competitive enzyme-linked immunosorbent assay (icELISA) based on monoclonal antibodies (2G4C7) was developed. Because the icELISA had higher sensitivity to the hydrolysate ( Hapten A ) of fenpyroximate, a method for indirectly determining the concentration of fenpyroximate was established by measuring the content of Hapten A . The assay had a working range of 0.07–1.49 ng/mL and a half-maximal inhibitory concentration (IC 50 ) of 0.34 ng/mL. It showed average recoveries of 77.0%–105.4 %, 72.3%–106.4 % in citrus and apple samples, respectively. The icELISA and UPLC-MS/MS test results for samples of various citrus cultivars are remarkably consistent. These results and data represent the icELISA is suitable and applicable for detecting fenpyroximate residuals in fruit and vegetable samples.
研究领域:
分析化学
·
环境科学
·
农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Yaping Duan,Congmin Liu,Xiaowen Dou,Liu Wu,Hao Liu,Linan Shan,Shihai Yang,Jiaoyang Luo,Meihua Yang
Triptolide is a characteristic chemical component and quality marker of Tripterygium wilfordii Hook. F., a widely used traditional Chinese medicine with various pharmacological activities. Triptolide-14-succinate is a derivative of triptolide that can be combined with bovine serum albumin and ovalbumin to form an immunogen and coating antigen. Monoclonal antibody specific to triptolide-14-succinate conjugated with ovalbumin was produced and named mAb 2D10 1H8. An indirect competitive enzyme-linked immunosorbent assay (icELISA) was established by using mAb 2D10 1H8. The 50% inhibition concentration and working range of triptolide for the icELISA were 0.28 and 0.09–0.87 μg mL −1 , (based on 20–80% binding inhibition of mAb 2D10 1H8 to triptolide, R 2 = 0.9997, y = -0.571Logx + 0.246), respectively. The icELISA displayed crossreactivity values of 48.6, 20.5, 3.27, and < 1.00% for triptonide, triptophenolide, tripterifordin, and other analogs of triptolide, respectively. The average recovery of triptolide from the Tripterygium wilfordii samples as determined by icELISA ranged from 90.05 to 106.6%. Triptolide was detected in 38 batches of Tripterygium wilfordii samples by icELISA, which was validated by ultra-fast liquid chromatography combined with electrospray ionization tandem-mass spectrometry. In conclusion, the icELISA method established in the present study is applicable for rapid authentication of Tripterygium wilfordii and related products.
研究领域:
中药学
·
免疫学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Zihui Jin,Di Sun,Yifan Liu,Bo Xu,Yaqi Jin,Qiyang Zhao,Yue He,Jing Li,Yaohai Zhang,Yongliang Cui
Tangeretin, a polymethoxyflavone abundant in citrus fruits, is acclaimed for its diverse biological benefits to human health and is a significant component of traditional Chinese medicine. This study reports the development of an indirect competitive enzyme-linked immunosorbent assay (icELISA) with monoclonal antibody for quantifying tangeretin in various citrus and herbal medicines. The optimized icELISA demonstrated a half-maximal inhibitory concentration (IC 50 ) of 5.57 ng/mL, encompassing a dynamic range of 1.54–25.38 ng/mL, and showcased average recoveries ranging from 81.65 % to 105.75 % in citrus peel and pulp samples. Extensive analysis of six citrus cultivar samples and four herbal samples was conducted using the developed assay, with results corroborated by Ultra–Performance Liquid Chromatography Tandem Mass Spectrometry (UPLC–MS/MS). These findings confirm the assay’s efficacy and reliability for tangeretin detection, offering a practical method for evaluating this bioactive compound in food.
研究领域:
食品科学
·
免疫学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 16
·1911-1918
·
IF 2.896
Hui-Ling Wu,Bing-Zhi Wang,Yu Wang,Zhi-Li Xiao,Lin Luo,Zi-Jian Chen,Yu-Dong Shen,Zhen-Lin Xu
Diazinon (DAZ) is an organophosphorus pesticide (OP), which is commonly used to prevent and control harmful pests that endanger agricultural products. In this study, we developed a novel heterology coating strategy for the immunoassay of DAZ. The DAZ coating hapten can be directly conjugated to the carrier protein without requiring a spacer arm. This proposed hapten coating strategy is time-saving and significantly improves the sensitivity of the immunoassay due to the lack of a spacer arm. The as-synthesized coating antigen was used to screen the monoclonal antibody (mAb). Finally, the developed indirect competitive enzyme-linked immunoassay (icELISA) showed IC50 and limit of detection (LOD) values of 0.58 ng mL−1 and 8 pg mL−1, respectively. This method exhibited negligible cross-reactivity towards other analogues, and the recoveries of samples (cucumber, cabbage, and lettuce) ranged from 92.6% to 125.4%, with coefficients of variance (CV) below 12%. Good correlation between icELISA and high-performance liquid chromatography mass spectrometry (HPLC-MS/MS) was obtained. The proposed icELISA was an ideal tool for monitoring DAZ residues in food samples.
研究领域:
农业化学
·
分析化学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Yifan Liu,Zihui Jin,Di Sun,Jiexin Zheng,Bo Xu,Tianyu Lan,Qiyang Zhao,Yue He,Jing Li,Yaohai Zhang,Yongliang Cui
Both rhoifolin and diosmin belong to flavonoids, which are widely present in citrus. Diosmin is not only used in the medical field in the world, but also used as a dietary supplement in the United States. Rhoifolin has a similar structure to diosmin and also exhibits antioxidant and anti-inflammatory properties. In this study, an anti-rhoifolin monoclonal antibody was prepared and an indirect competitive enzyme-linked immunosorbent assay (icELISA) method was established. The half-maximal inhibitory concentration (IC 50 ) of icELISA was determined to be 4.83 ng/mL, and the detection range was 0.97–33.87 ng/mL. The results of UPLC-MS/MS and icELISA generally demonstrate consistency. Moreover, by exploiting the cross-reactivity of the antibody, diosmin in tablets can be detected by icELISA. The results demonstrate that the developed method has good accuracy, reproducibility, and broad application prospects.
研究领域:
药理学
·
分析化学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Food Science & Technology
·
2022
·
IF 0
Miao Li,Jingjie Huang,Yingxian Chen,Yuejiao Ma,Sixuan Qian,Zhixuan Sun,Jianzhong Shen,Jiancheng Li
Ractopamine (RAC) is a feed additive that regulates protein synthesis. In this study, an indirect enzyme-linked immunosorbent assay (icELISA) for the detection of RAC residues in the porcine liver was developed based on our specific immunomagnetic bead (IMB) coated with ractopamine-single chain variable fragment (RAC-scFv). The intact RAC-scFv was constructed, and its key amino acid sites were analyzed. Finally, an icELISA based on immunomagnetic beads separation techniques (IMBs) of RAC from the porcine liver was established with an IC50 of 9.15 ng/mL and a linear range from 4.8 to 17.4 ng / mL. The IC50 of the prepared RAC-scFv was 8.7 ng/mL with a linear range of 3.8 to 19.6 ng/mL. The limit of detection (LOD) in the porcine liver of RAC was 4.57 μg/kg, and the recoveries ranged from 72.65 to 89.41% (CV < 15%).
研究领域:
分析化学
·
兽医学
·
食品安全
✓ 已验证
DOI
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2024
·
IF 1.7
Jiaxin Wang,Wenhua Shang,Shibei Shao,Jialin Zhang,Qiuyang Wu,Min He,Licai Ma,Jianzhong Shen,Zhanhui Wang,Kai Wen
Paxilline (PAX) is a tremorgenic mycotoxin that is thought to be associated with various syndromes in animals. To facilitate the determination of this toxin, a hapten was chemically synthesized and then respectively conjugated to Bovine Thyroglobulin (BTG) and Bovine serum albumin (BSA) for antibody production. Monoclonal antibody (mAb) 4F8 was finally obtained and used to establish an accurate and sensitive indirect competitive ELISA (icELISA). Various parameters that could impact the performance of icELISA were optimized, including the ion concentration, pH value and organic solvent. Under optimal condition, this assay exhibits an IC50 value as low as 0.84 µg L−1. The limit of detection (LOD) of the established icELISA method for PAX was 0.03–0.3 µg L−1. In conclusion, this study provided a rapid, sensitive, and high-throughput immunoassay for the detection of PAX in grains.
研究领域:
免疫学
·
食品科学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Chang Liu,Yuan Chen,Hong Lin,Limin Cao,Kaiqiang Wang,Xiudan Wang,Jianxin Sui
ABSTRACT Low specificity and sensitivity often restrict the fluoroquinolones (FQs) detection when traditional IgG antibodies are used as recognition elements in immunoassay. Herein, shark-derived single-domain antibodies (ssdAbs) were used as new recognition elements to enhance the sensitivity and specificity for norfloxacin detection in immunoassay. Firstly, NOR-specific clones were isolated by phage display technology. Finally, five clones were successfully isolated from a NOR-immunized library. Secondly, a soluble expression system was constructed to produce ssdAbs. The 1N9 ssdAb exhibits the best affinity to the NOR complete antigen, with a limit of detection of 0.392 ng/mL and the cross-reaction rates of less than 8.240%, indicating its high sensitivity and specificity as a new recognition element in NOR detection. More importantly, the ELISA result proved that in NOR-negative fish matrix, no serious interference was observed, the 1N9 ssdAb could still recognize complete antigen NOR-OVA effectively. While in icELISA, 1N9 ssdAb could recognize NOR in different NOR-spiked concentrations between 10 to 1000 ng/mL, with the recovery rates between 93.66 - 116.49% with high reproducibility. Our study provides a new and effective recognition element with great potential for NOR detection.
研究领域:
免疫学
·
分析化学
·
药学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL LETTERS
·
2024
·
IF 1.6
Hui-Ling Wu,Jing Chen,Fei Pan,Qi-Sheng Xia,Shi-Wei Zhang,Xue-Jian Chen,Rong-Hu Feng,Gui-Hong Zhang,Lei Liu,Xin-Tian Lai,Pan Tan
Pseudomonas cocovenenans subsp. farinofermentans is a pathogenic bacterium that causes the poisoning of fermented rice products. Its isomeric products, bongkrekic acid (BA) and isobongkrekic acid (IBA), have become popular research topics. In this study, we developed a heterologous-coating strategy for high-sensitivity indirect competitive enzyme-linked immunoassay (icELISA) for BA and IBA using monoclonal antibodies (mAbs). The optimized procedure exhibited an IC50 of 5.49 ng/mL, a limit of detection of 0.99 ng/mL, and a linear range from 2.12 to 14.21 ng/mL. Notably, this method exhibited negligible cross-reactivity to other small toxins, and the recoveries of the samples, including rice noodles, Auricularia auricula, and flat rice noodles, ranged from 92.0%–111.9%, 105.6%–115.0%, and 83.2%–109.0%, respectively, with coefficients of variation below 12%. A comparison of the recoveries of high-performance liquid chromatography-mass spectrometry (HPLC-MS) and icELISA demonstrated that the developed approach provides good accuracy and reliability. Overall, the simple preparation of IBA combined with the suitability of icELISA for high-throughput analysis is efficient and cost-effective, making it suitable for routine screening of food.
研究领域:
食品科学
·
微生物学
·
分析化学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Di Sun,Yifan Liu,Zihui Jin,Bo Xu,Yaqi Jin,Qiyang Zhao,Yue He,Jing Li,Yaohai Zhang,Yongliang Cui
Limonin is an intensely bitter and highly oxidized tetracyclic triterpenoid secondary metabolite, which is abundant in the Rutaceae and Meliaceae, especially in Citrus. In order to detect limonin content in complex substrates such as citrus and traditional Chinese medicine, monoclonal antibodies specifically recognizing limonin were prepared and an indirect competitive enzyme-linked immunosorbent assay (icELISA) was established. The median inhibition concentration (IC 50 ) was 5.40 ng/mL and the linear range was 1.25–23.84 ng/mL. The average recoveries from citrus peel and pulp samples were 95.9%–118.8% and 77.5%–113.1%, respectively. Moreover, the contents of limonin in 6 citrus samples and 4 herbal samples were analyzed by icELISA and UPLC-MS, and the results of the two methods were consistent. This validation is sufficient to demonstrate that the developed immunoassay is applicable for the detection of limonin in citrus and herbal samples and has the advantage of high efficiency, sensitivity, and convenience.
研究领域:
分析化学
·
免疫学
·
食品科学
·
生药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2026
·
IF 6.7
Chunlei Cao,Feng Sun,Yajing Hou,Jiayi Gao,Qiyang Zhao,Yue He,Yaohai Zhang,Yongliang Cui
Psoralen and isopsoralen are the key active components and quality control standards of traditional Chinese medicines such as Psoraleae Fructus. In this study, two psoralen haptens were designed and synthesized, and a psoralen monoclonal antibody (mAb 4E6) was successfully screened, which could simultaneously recognize isopsoralen with a cross-reactivity rate of 9.85%. Based on this antibody, an indirect competitive enzyme-linked immunosorbent assay (icELISA) was established for the determination of psoralen and total psoralen and isopsoralen. The half-maximal inhibitory concentration (IC 50 ) values were 34.35 ng/mL and 152.22 ng/mL, with the working ranges of 4.22-328.92 ng/mL and 43.14-624.44 ng/mL, respectively. Recovery experiments in vitamin C tablets and Cassia seed matrices yielded recovery rates of 87.6%-93.2% and 81.6%-102.6%, respectively, demonstrating good precision. When applied to the analysis of four proprietary Chinese medicine samples, the results obtained by this method were in strong agreement with those from the reference UPLC-MS/MS method (R 2 = 0.9957). This assay offers operational simplicity and reliable accuracy, making it well-suited for rapid screening and quality assurance of psoralen-type compounds in complex samples.
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Jing Zhao,Yaohai Zhang,Qiyang Zhao,Yue He,Zhixia Li,Aihua Chen,Chengqiu Wang,Baomin Wang,Bining Jiao,Yongliang Cui
Naringenin, a flavonoid compound found in pummelo, is a key biological active compound in some traditional Chinese medicines, including Citri reticulatae pericarpium , Citri reticulatae pericarpium viride , Aurantii fructus immaturus , and Aurantii fructus . These Chinese medicinal preparations are the peels or immature fruits of certain citrus species. Aiming at detecting naringenin in complex matrices such as pummelo and traditional Chinese medicines, we put forward a sensitive and practical indirect competitive enzyme-linked immunosorbent assay (icELISA) based on anti-naringenin monoclonal antibodies (anti-Nar-mAbs). The median inhibitory concentration (IC 50 ) was 4.43 ng/mL, and the working range was 1.15–15.81 ng/mL. The findings of the icELISA for the analysis of naringenin in pummelo and herb samples had a good correlation with the ultra performance liquid chromatography (UPLC) methodology and showed good accuracy and reproducibility. These data demonstrated that the developed icELISA is reliable, accurate, and suitable for detecting naringenin in pummelo and traditional Chinese medicines.
研究领域:
药理学
·
分析化学
·
中药学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）