PEST MANAGEMENT SCIENCE
·
2025
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IF 3.8
Haoming Guo,Nan Xiao,Zhixuan Wang,Yabo Liang,Xueke Liu,Peng Wang,Donghui Liu
BACKGROUND Indoxacarb, a type of chiral pesticide, is used to control Lepidoptera insects. Most studies had focused on the environmental behavior and selective toxicity of indoxacarb enantiomers, and the mechanism behind its selective biological activity against target organisms is not well understood. RESULTS Spodoptera frugiperda was selected as the target insect. The lethal concentrations (96 h) of indoxacarb enantiomers on S. frugiperda were 2.61 mg/kg ( S -indoxacarb) and 463.52 mg/kg ( R -indoxacarb). S -Indoxacarb resulted in stronger oxidative damage to S. frugiperda than R -indoxacarb, and catalase and malondialdehyde were upregulated by 40.46% and 68.64% respectively after treatment with S -indoxacarb. Furthermore, cytochrome P450 and carboxylesterase were activated by S -indoxacarb, increasing by 39.62% and 63.68% respectively. Decarbomethoxyllated JW062 (DCJW), a metabolite of indoxacarb, has insecticidal activity. The concentration of DCJW in the S -indoxacarb treatment group was 2.73 times that in the R -indoxacarb treatment group. Molecular docking results demonstrated that S -indoxacarb could spontaneously bind to metabolic enzymes and be metabolized. CONCLUSIONS Enantiomeric bioactivity of indoxacarb enantiomers against on S. frugiperda was observed. S -Indoxacarb demonstrated remarkable insecticidal efficacy. Upon ingestion by S. frugiperda , it induced oxidative damage. Furthermore, S -indoxacarb was metabolized to DCJW, which has a significant role in its insecticidal properties. The selective bioactivity of indoxacarb enantiomers in S. frugiperda might be attributed to the enantiomeric metabolites. These findings offer a new perspective on the selective mechanisms of chiral pesticides. © 2025 Society of Chemical Industry.
研究领域:
昆虫学
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农药科学
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生物化学
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DOI
PubMed
高影响因子
Applied Food Research
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2025
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IF 6.2
Ai Liu,Fengyu Li,Jiacheng Ge,Guodong Si,Xianzheng Han,Yun Gao,Wenli Wang,Li Su,Nan Zou,Wei Mu,Xiaomei Li,Xiuyu Pang
This study focuses on the development and validation of a detection method for indoxacarb residues in three major vegetable crops, Chinese cabbage, cabbage, and tomato, which are widely cultivated worldwide. The method was rigorously validated through field trials and dietary risk assessment. A strong linear response was observed for indoxacarb at the concentrations of 0.0005 - 0.25 mg/L, with a limit of quantitation of 0.01 mg/kg. The half-life of indoxacarb ranged from 4.61 to 6.89 days in Chinese cabbage, 3.06 to 6.21 days in cabbage, and 2.28 to 3.46 days in tomato. The terminal residues were <0.01 - 0.2 mg/kg in Chinese cabbage, <0.01 - 0.15 mg/kg in cabbage, and <0.01 - 0.029 mg/kg in tomato. Dietary risk assessment revealed a low probability of long-term risk (12.9 %) associated with the consumption of these crops. Overall, the study suggests that the use of indoxacarb on these three vegetables is safe for the general population, with minimal risk even under long-term exposure.
研究领域:
农学
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食品安全
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环境科学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
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IF 4.784
Hongyun Zhang,Ying Xia,Mingpeng Zhou,Jianyong Zheng,Zhao Wang,Yinjun Zhang
An intracellular esterase (BCE) from Bacillus cereus WZZ006 was purified to homogeneity with an 89.5-fold purification , specific activity of 1.79 U/mg, and 26.7% recovery. The estimated molecular weight of BCE was 96 kDa which was analyzed by SDS-PAGE and MALDI-TOF-MS. Activity staining denotes that BCE has an unexplored new carboxyl esterase characteristic. BCE enzyme activity was maximum at pH 8.5 and also at 50 °C with p NP-caproate as a substrate. This indicates that the studied BCE as a thermoalkaliphilic esterase. The kinetic properties like K m , V max , k cat and k cat/ K m value for BCE was found to be 0.98 mM, 0.03 mM/min, 69.47 min −1 and 70.89 mM −1 min −1 , respectively. Synthesis of ( S )-5-chloro-1-oxo-2,3-dihydro-2-hydroxy-1H-indole-2-carboxylic acid methyl ester (( S )-CODHCM) by BCE can be shortened to 3 h compared to 36 h with whole-cell catalysis. The e.e. s achieved was 93.83%, and conversion around 52.78% with E being 39.95. These features render BCE as a promising biocatalyst for the synthesis of a key chiral intermediate for indoxacarb.
研究领域:
生物化学
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酶工程
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微生物学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2021
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IF 9.381
Liupeng Yang,Amir E. Kaziem,Yigang Lin,Chao Li,Yuting Tan,Suqing Huang,Dongmei Cheng,HanHong Xu,Zhixiang Zhang
In this study, a pesticide controlled release system with dual response characteristics of pH and enzyme triggering was developed. Indoxacarb (IDC) was loaded into hollow mesoporous silica (HMS) nanoparticles , carboxylated β-cyclodextrin (β-CD) acted as a capping molecule to couple with the amino-functionalized HMS, and their well-defined morphological structures were confirmed by scanning electron microscopy and transmission electron microscopy . The results showed that the prepared IDC loaded HMS-CD had high loading efficiency (26.42%, w/w) and showed excellent dual response properties to pH and the α-amylase enzyme. IDC loaded HMS-CD nanoparticles showed better insecticidal activity against Spodoptera frugiperda than applying the same dose of IDC emulsifiable concentrate, and the toxicity of IDC loaded HMS-CD to zebrafish was reduced by more than 5-fold, indicating that insecticide delivery systems based on β-CD-anchored HMS nanoparticles could potentially be applied for sustainable control of pests and reduce harm to non-target organisms and the environment.
研究领域:
农业科学
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环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2022
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IF 4.966
Fen Li,Xueyan Gong,Linlin Yuan,Xuelian Pan,Haifeng Jin,Rongcai Lu,Shaoying Wu
Liriomyza trifolii , which has been recently prevalent in China, harms more than 300 plant species, especially cowpea in Hainan. This pest also affects the quality and production of vegetables in winter. Indoxacarb is the first commercial oxadiazine pesticide , which is a new efficient insecticide used to control pests of Diptera, including L. trifolii . The unique mechanism of indoxacarb is that indenyl is transformed into N-demethoxycarbonyl metabolite (DCJW) in insects and acts on inactivated sodium channel; DCJW could then destroy the conduction of nerve impulses , which leads to movement disorders, feeding stoppage, paralysis, and eventually the death of pests. The field population of L. trifolii developed resistance by 769 times higher than the sensitive population in Sanya, Hainan. Results revealed the existence of a mutation (i.e., V1848I) in the sixth transmembrane segment of Domain IV of the sodium channel in the field population. The homozygous resistant genotype frequency for the V1848I mutation was 10–15% among the three field-collected populations. This paper reports for the first time the presence of the kdr mutation V1848I in resistant populations of L. trifolii to indoxacarb. The present study will contribute to the understanding of the evolution of indoxacarb resistance and contribute to the development of resistance management practices for winter vegetables in Hainan.
研究领域:
昆虫学
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农药科学
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农业科学
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分子生物学
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DOI
PubMed
高影响因子
Materials Today Chemistry
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2025
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IF 6.7
Qun Zheng,Xiaofeng Xie,Suqing Huang,Jiao Ding,Nanhe Huang,Zhixiang Zhang
Feather keratin (FK) is a natural polymer material derived from discarded feathers with excellent modifiability and functionalization properties. However, the current applications in agriculture, especially in the development of nanopesticides targeting pest control, are still relatively limited. In this study, an insecticide controlled release system with pH and reduced glutathione (GSH) responsive properties was designed and prepared. The coating coverage was carried out by combining the carboxyl group of FK with aminated hollow mesoporous silica (HMS), which was loaded with indoxacarb (IDC) to obtain IDC@FK-HMS. IDC@FK-HMS had an average particle size of 178 nm, achieved a drug loading of 23.22 %, and exhibited superior thermal and photostability. Compared to IDC bait, IDC@FK-HMS bait has more significant insecticidal activity against red fire ants. The mortality of red fire ants fed with 8 mg/kg of bait for 72 h were 52.22 ± 1.92 % and 66.67 ± 3.34 %, respectively. In addition, IDC@FK-HMS had a more significant effect on the attack ability of red fire ants, which required more time to prey on yellow mealworms. IDC@FK-HMS was able to reduce the alertness and detoxification enzyme activity of red fire ants and enhance their sensitivity to insecticides, which in turn enhanced the insecticidal activity. FK-HMS encapsulation did not alter the toxic effects of IDC, both of which caused significant impacts on the midgut and acetylcholinesterase activity of red fire ants. The study enriched the avenues of application of FK in agriculture, especially in pest control, and provided new insights into the green and sustainable control of red fire ants.
研究领域:
纳米技术
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农药科学
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农业化学
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材料科学
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昆虫学
✓ 已验证
产品关联
DOI
高影响因子
PEST MANAGEMENT SCIENCE
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2023
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IF 4.1
Yifan Li,Shujun Ni,Yunping Wang,Ruichi Li,Hong Sun,Xuan Ye,Zhen Tian,Yalin Zhang,Jiyuan Liu
BACKGROUND Insecticide resistance continuously poses a threat to agricultural production. Chemosensory protein-mediated resistance is a new mechanism of insecticide resistance discovered in recent years. In-depth research on resistance mediated by chemosensory proteins (CSPs) provides new insight into aid insecticide resistance management. RESULTS Chemosensory protein 1 in Plutella xylostella ( PxCSP1 ) was overexpressed in the two indoxacarb-resistant field populations and PxCSP1 has a high affinity with indoxacarb. PxCSP1 was upregulated when exposed to indoxacarb and the knockdown of this gene elevated sensitivity to indoxacarb, which demonstrate that PxCSP1 is involved in the indoxacarb resistance. Considering that CSPs may confer resistance in insects via binding or sequestering, we explored the binding mechanism of indoxacarb in PxCSP1 -mediated resistance. Using molecular dynamics simulations and site-directed mutation, we found that indoxacarb forms a solid complex with PxCSP1 mainly through van der Waals interactions and electrostatic interactions. Between these, the electrostatic interaction provided by the Lys100 side chain in PxCSP1 , and especially the hydrogen bonding between the NZ atom and the O of the carbamoyl carbonyl group of indoxacarb, are the key factors for the high affinity of PxCSP1 to indoxacarb. CONCLUSIONS The overexpression of PxCPS1 and its high affinity to indoxacarb is partially responsible for indoxacarb resistance in P. xylostella . Modification of indoxacarb's carbamoyl group has the potential to alleviate indoxacarb resistance in P. xylostella . These findings will contribute to solving chemosensory protein-mediated indoxacarb resistance and provide a better understanding of the insecticide resistance mechanism. © 2023 Society of Chemical Industry.
研究领域:
昆虫学
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分子生物学
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农药科学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
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2024
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IF 5.6
Jianguo Feng,Qianwei Liang,Zhiyang Chen,Yifei Tan,Tianzhen Jiang,Sa Dong
The design and development of stimuli-responsive nanocarriers hold the promise of the smart delivery of pesticides to target organisms. Herein, we successfully prepared mesoporous silica nanoparticles using the self-templating method, which were grafted with both N-isopropylacrylamide (NIPAM) and cyclodextrin (CD) and loaded with indoxacarb (IN) to afford a pesticide release system with a dual-stimulated response of temperature and α-amylase, referred to as IN@MSNs@NIPAM@CD. Physicochemical characterisation revealed the successful preparation of IN@MSNs@NIPAM@CD with 46.30 % indoxacarb loading. The results showed that IN@MSNs@NIPAM@CD was able to release IN rapidly in the environment of high temperature and α-Amylase, with excellent dual-responsive property. The UV resistance evaluation showed that IN@MSNs@NIPAM@CD could effectively improve the photodegradation resistance of indoxacarb. Fluorescent inverted microscope showed that FITC@MSNs@NIPAM@CD has excellent uptake and translocation properties in corn plants. Bioactivity tests showed that at 14 d, IN@MSNs@NIPAM@CD (LC 50 = 9.83 mg L −1 ) exhibited higher insecticidal activity and longer effective duration than IN@EC (LC 50 = 14.64 mg L −1 ). Acute toxicity test revealed that the IN@MSNs@NIPAM@CD nanoparticles were less acutely toxic to earthworms, zebrafish, BEAS-2B cells and MSNs@NIPAM@CD nanocarriers were safe for the growth of corn. Thus, the dual-responsive nano-controlled-release formulation of IN@MSNs@NIPAM@CD can provide an effective way of achieving the precise delivery and reduced use of pesticides.
研究领域:
农业科学
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材料科学
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昆虫学
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产品关联
DOI
高影响因子
npj Science of Food
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2025
·Vol. 9
·期 1
·1-8
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IF 7.8
Pan Hui,Zhang Wei,Jin Meng,Zhang Fang,Chen Xuelei,Meng Xiao,Shao Hua,Song Liqun,Zhang Zhihu,Wang Cuijuan
Concerns over health risks associated with mixed pesticide residues have led to a demand for rapid analytical methods. We present a portable surface-enhanced Raman spectroscopy (SERS) device specifically designed for real-time monitoring of indoxacarb (INDX) and chlorfenapyr (CFP) in vegetable juice. This point-of-use sensor integrates silver nanoparticles with a metal-organic framework (Ag/MOF-808(Zr)) and employs a 19-well quartz plate alongside a portable Raman spectroscopy instrument, facilitating simultaneous adsorption, separation, and detection. Detection limits for INDX and CFP in ginger and spinach juices were 0.26–0.76 ppb. Notably, results closely matched those from liquid chromatography, and in-situ detection was achieved within 2 min without pre-treatment, significantly enhancing efficiency over conventional chromatographic methods. This innovative approach demonstrates substantial potential for detecting pesticide mixtures in non-laboratory settings, presenting a valuable option for point-of-use applications.
研究领域:
分析化学
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食品安全
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环境监测
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纳米技术
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光谱学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2026
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IF 5.4
Jianguo Feng,Zhen Li,Zhiyang Chen,Tianzhen Jiang,Xiang Li,Ziyi Wu
Stimuli-responsive pesticide nanocarriers allow precise delivery of pesticides to target pests, enhancing insecticidal efficacy while improving safety for non-target organisms. Herein, mesoporous silica nanoparticles (MSNs) were synthesized via the sol–gel method, followed by encapsulation of indoxacarb (IN) within their pores. Disulfide bonds (SS) were grafted onto the MSN surface, and cyclodextrin (CD) was incorporated to obtain enzyme/redox dual-responsive nanoparticles (IN@MSNs@SS@CD). Physicochemical characterization revealed a well-defined spherical core–shell structure. The encapsulated IN content was approximately 22.27%. In the presence of α-amylase and glutathione, the highest cumulative release (72.9%) occurred at 7 days, demonstrating dual-responsive release behavior. The contact angle and resistance to rain erosion experiments show that the nanoparticles have good wettability and adhesion. Insecticidal activity tests showed that IN@MSNs@SS@CD caused higher mortality in Cnaphalocrocis medinalis than commercial IN@EC. Moreover, the nanoparticles exhibited enhanced biocompatibility toward earthworms, zebrafish, and human bronchial epithelial cells (BEAS-2B), supporting their environmentally benign potential. Herein, a dual-responsive nanoparticle that can be used for precise pest management to improve biosafety and wettability has been developed. It provides a transformative approach for precision agriculture.
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产品关联
DOI
高影响因子
Biology-Basel
·
2022
·Vol. 11
·期 12
·1718
·
IF 5.168
Muhammad Hafeez,Xiaowei Li,Farman Ullah,Zhijun Zhang,Jinming Zhang,Jun Huang,Limin Chen,Junaid Ali Siddiqui,Xiaoyun Ren,Shuxing Zhou,Muhammad Imran,Mohammed A. Assiri,Myron P. Zalucki,Yonggen Lou,Yaobin Lu
Simple SummaryThe fall armyworm (FAW),Spodoptera frugiperda(J.E. Smith), is a voracious insect pest native to the Western Hemisphere, particularly in South America. The polyphagous fall armyworm feeds on more than 350 plants in such families, including Poaceae, Asteraceae, and Fabaceae. Transgenic plants that expressBacillus thuringiensisproteins (Bt plants) and synthetic insecticides are the main tactics to controlSpodoptera frugiperda, although widespread usage of synthetic chemicals has resulted in the emergence of resistance. We assessed cross-resistance, resistance mechanism, and fitness costs based on the life history traits ofSpodoptera frugiperda. Results indicated that after 24 generations of selection, the resistance to indoxacarb was increased by 472.67-fold as compared to the Ind-UNSEL. Significantly longer developmental time of larvae extended pupal duration, shorter adult longevity, and lower fecundity were observed in Ind-SEL as compared with the Ind-UNSEL population. Butoxide synergist increased susceptibility to indoxacarb, indicating that P450 enzymes may be involved in indoxacarb resistance. Therefore, it is crucial that we comprehend how insecticides work and how resistance develops in order to develop techniques for managing resistance. These data are valuable to understand the indoxacarb resistance mechanism and provides vital information for scientific-based guidance of pest management decisions.AbstractThe fall armyworm,Spodoptera frugiperda(J.E. Smith), is a voracious insect pest that is difficult to control due to resistance to insecticides and Bt proteins. We assessed cross-resistance, resistance mechanism, and fitness costs based on the life history traits ofS. frugiperda. We established anS. frugiperdastrain selected for resistance to indoxacarb (Ind-SEL) from a field-collected population and an unselected strain, Ind-UNSEL. Results indicated that after 24 generations of selection, the resistance to indoxacarb was increased by 472.67-fold as compared to the Ind-UNSEL. There was high cross-resistance to deltamethrin (31.23-fold) with very low or negligible cross-resistance to chlorantraniliprole, emamectin benzoate, and/or methoxyfenozide in the Ind-SEL population. Butoxide synergist increased susceptibility to indoxacarb, indicating that P450 enzymes may be involved in indoxacarb resistance. Significantly longer developmental time of larvae extended pupal duration, shorter adult longevity, and lower fecundity were observed in Ind-SEL as compared with the Ind-UNSEL population. The Net reproductive rate (R0) was the only growth parameter that differs between crosses of Ind-SEL♂ × Ind-UNSEL♀ (176 ± 46) and Ind-SEL♀ × Ind-UNSEL♂ (328 ± 57). On the other hand, all population growth parameters differ between Ind-SEL and Ind-UNSEL strains. Our work contributes to the growing body of research that demonstrates the importance of strain genetics in fitness cost experiments and helps resistance management programs make decisions.Keywords:Spodoptera frugiperda;indoxacarb selection;cross-resistance;inheritance of resistance;fitness cost
研究领域:
昆虫学
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害虫管理
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
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2023
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IF 7.7
Yongpan Shan,Yajie Ma,Changcai Wu,Xiangliang Ren,Xianpeng Song,Dan Wang,Hongyan Hu,Xiaoyan Ma,Yan Ma
Control release of pesticides based on target plant characteristics is highly desirable for improving the effective utilization rate and reducing non-target organism risks. Here, the controlled release formulations (Cu-CS@IN@MIL-100 (Fe)) were successfully prepared by employing MIL-100 (Fe) as the carrier material, indoxacarb (IN) as the model pesticide, and chitosan (CS) as a coating to chelate copper ions by chemical crosslinking. The prepared Cu-CS@IN@MIL-100 (Fe) had an acceptable loading content (36.25 %). The introduction of CS and copper ions endowed it with sustained release properties under weak acidity conditions like cotton leaves. The laboratory bioassay experiments showed that Cu-CS@IN@MIL-100 (Fe) had practical good insecticidal activity (LC 50 = 4.936 mg/L) in controlling Helicoverpa armigera larvae. For long-term control efficacy experiments, after 14 days of application, the Cu-CS@IN@MIL-100 (Fe) treatment (73 %) mortality of Helicoverpa armigera larvae was significantly higher than the 15 % indoxacarb suspension concentrates (SC) treatment (27 %). Moreover, Cu-CS@IN@MIL-100 (Fe) had no adverse effect on cottonseed germination and the acute toxicity to Chrysopa pallens larvae was significantly lower than IN SC. Therefore, MIL-100 (Fe) could be used as an ecological safety carrier for pesticide delivery to improve IN effective utilization and reduce non-target organism risks.
研究领域:
农业科学
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农药科学
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环境科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）