Immobilized lipase based on SBA-15 adsorption and gel embedding for catalytic synthesis of isoamyl acetate
In this study, Candida rugosa lipase (CRL) was immobilized on the silica carrier SBA-15 by physical adsorption , then mixed with hydrophobically modified sodium alginate and dropped into calcium chloride solution. Finally, the adsorption-embedding immobilized lipase (L-SBA-15-Mgel) was successfully prepared. The conformation of the carrier before and after lipase immobilization were analyzed by scanning electron microscopy (SEM), thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FT-IR) and other characterization techniques. The molecular docking and molecular dynamics simulation of CRL and modifier dodecyl glycidyl ether (DGE) ligand were carried out to analyze the potential influence of modifier on enzyme characteristics. The basic enzymatic properties including the activity, stability and reaction kinetic parameters of the immobilized lipase L-SBA-15-Mgel were studied. L-SBA-15-Mgel showed good storage stability and reusability. After 16 days of storage, the activity retention was 47.85%, and there was still 63.20% of the residual activity after seven cycles of reuse. The immobilized lipase was applied to the efficient synthesis of food flavor ester isoamyl acetate . Under the reaction conditions of 50 °C and 200 rpm, the highest yield of isoamyl acetate catalyzed by L-SBA-15-Mgel was 85.19%.
危险品化学品经营许可证（带存储）