JOURNAL OF FOOD SCIENCE
·
2023
·Vol. 88
·期 3
·977-987
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IF 3.9
Wenfu Hou,Yile Lin,Yujie Zhang,Tingting Liu,Juan Du,Yang Yi,Hongxun Wang
This study investigated the effect of 0.8% (m/v) kojic acid treatment on changes in the microstructure and myofibrillar protein of duck meat covered with oxygen-permeable polyvinylchloride (PVC) film (9 ± 0.5 µM) during superchilled storage (−1.65 ± 0.5°C). The superchilled samples exhibited wider gaps between muscle fibers at 5 weeks storage compared with kojic acid–treated groups. Based on the variation of water status, the water-holding capacity decreased significantly ( p < 0.05), and bound water and immobilized water were gradually converted into free water during superchilled storage. For kojic acid–treated samples, however, no major changes were observed with respect to muscle structure, water status, and protein degradation at 6 weeks. The 0.8% (m/v) kojic acid treatment increased the water-holding capacity, reduced carbonyl content and protein degradation, and decreased the α-helix contents loss of myofibrillar proteins. Kojic acid treatment effectively protected myofibrillar protein structure from being destroyed during superchilled storage, suggesting that this method was a good way to reduce protein oxidation and prolonged its shelf life.
研究领域:
食品科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2025
·
IF 6.2
Yun Ma,Yi Xie,Hui Liu,Yadan Luo,Baojie Liu,Chengrong Qin,Chen Liang,Caoxing Huang,Shuangquan Yao
In this study, eucalyptus hemicellulose was separated using kojic acid (KA), which contains hydroxyl and ketone groups. The maximum selectivity and hemicellulose separation yield were obtained (KA concentration of 10 %, 160 °C, and 50 min). The xylose separation increased from 79.36 ± 2.93 % to 84.36 ± 2.95 % after the KA pretreatment. The lignin separation decreased by 9.56 ± 0.06 % and 4.81 ± 0.26 % compared with acetic acid (AA) and salicylic acid (SA) pretreatment. It had the lowest condensed and highest noncondensed phenolic hydroxyl contents. The depolymerization and condensation of lignin were inhibited by the ketone group in KA. A synergistic effect of the hydroxyl acid and ketone groups on the efficient and selective separation of hemicellulose was discovered. This study provides a new approach for pretreating hemicellulose using novel multifunctional organic acids.
研究领域:
生物化学
·
材料科学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2021
·Vol. 56
·期 11
·6039-6051
·
IF 3.713
Ruifei Wang,Meimei Fang,Xinyi Hu,Yigang Yu,Xinglong Xiao
Summary The development of effective natural antibacterial agents is important due to the insecurity of synthetic antimicrobial agents and consumer preferences. In this study, kojic acid (KA) and tea polyphenols (TP) were found to exhibit synergistic inhibitory effect against Escherichia coli O157:H7 with a fractional inhibitory concentration index of 0.25. KA combined with TP at 25% of their minimum inhibitory concentrations (MICs) completely inactivated E. coli O157:H7 within 4 h. Subsequently, propidium iodide uptake tests, genomic DNA interaction analysis, molecular docking, field emission scanning electron microscopy and biofilm formation assay were conducted to understand the synergistic antibacterial mechanism of KA-TP. The results demonstrated that KA-TP attacked the cell membrane cooperatively, thus disturbing membrane integrity and cell structure, and KA could bind to the genomic DNA to affect the biofilm formation of E. coli O157:H7. Moreover, the contamination of E. coli O157:H7 on raw salmon fillets was eliminated to varying degrees after soaking treatments with KA and/or TP for 30 min. The depuration effect was further enhanced when KA combined with TP, and more than 2-log reduction of E. coli O157:H7 was obtained under the treatment of KA-TP at 2 MIC, suggesting that KA-TP may serve as a natural compound disinfectant for ready-to-eat aquatic products.
研究领域:
食品科学
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微生物学
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药理学
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DOI
高影响因子
FOOD CONTROL
·
2018
·
IF 3.667
Qifang Wu,Jun Xu,Huirong Xu
The attenuation or prevention of aflatoxins effect on agricultural products is still an unresolved problem due to the various influencing factors. Fluorescence fingerprint technique (such as excitation-emission matrix, EEM) has been developed for rapid detection of aflatoxins in liquid and solid samples. Unfortunately, the accuracy is seriously affected by background fluorescence compounds, such as kojic acid derivative (KAD). Therefore, development of more sensitive detection methods based on the enhancement of fluorescence signal is highly important. In this study, the EEM fluorescence technique was used to study the effects of α-, β-, and γ-cyclodextrins (CDs), and organic solvents on the fluorescence intensity of aflatoxin B 1 (AFB 1 ) and KAD, and molecular modeling study was also performed for the deeper understanding of these host-guest interactions. Results demonstrated that CDs and methanol could induce a higher fluorescence intensity of AFB 1 , while KAD presented a certain inhibitory effect on the enhancement of fluorescence signal of AFB 1 . Among the tested compounds β-CD formed the most stable conformation with AFB 1 (the logarithm of binding constant values are approximately 2.22–2.49) and the clathrate compounds caused the highest fluorescence enhancement of AFB 1 as well (the ratio of fluorescence intensity values ( I / I 0 ) for the presence and absence of CD at 430 nm are about 3.76–7.96). However, no obvious elevation for KAD fluorescence intensity caused by various CDs and organic solvents was observed at 493 nm. A significantly increase of fluorescence intensity was observed at the ratio of I 430 / I 493 by CDs. This suggested that CDs could be used to decrease the interference of KAD, and to improve the accuracy and sensitivity for detection of AFB 1 , especially for the low concentration level.
研究领域:
农业科学
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食品科学
·
分析化学
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DOI
高影响因子
TALANTA
·
2013
·
IF 3.498
Bing-bing Sun,Li Qi,Xiao-yu Mu,Juan Qiao,Ming-lin Wang
A facile chiral ligand exchange capillary electrophoresis (CLE-CE) system with Zn(II)- l -alanine as the chiral selector in the presence of β-cyclodextrin has been developed for enantioseparation of dansyl amino acids . The influence of the key factors, such as buffer pH, the ratio of Zn (II) to ligand, the concentration of β-cyclodextrin and the concentration of the complex, were investigated in detail when d , l -Tyr and d , l -Thr were selected as the model analytes. The proposed method showed favorable quantitative analysis property of dansyl d , l -tyrosine with good linearity ( r 2 ≥0.999) and reproducibility (RSD≤3.8%), then, it was applied in studying the activity of tyrosinase through the determination of l -tyrosine concentration variation after being incubated with the enzyme. Further, the inhibitory efficiency of kojic acid and soy sauce on the tyrosinase was investigated. The IC 50 of kojic acid obtained from the sigmoidal inhibitory curve was 21.35 μM. The results imply that the proposed CLE-CE system has the potential in exploring the activity of enzyme and screening the inhibitors of enzyme.
研究领域:
分析化学
·
生物化学
·
药学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2020
·
IF 5.065
Renren Sun,Haixia He,Yameng Wan,Lei Yuan,Liyuan Li,Jiao Sha,Gaoliang Jiang,Yu Li,Tao Li,Baozeng Ren
Using the laser monitoring method, the solubility of kojic acid (KA) in the four binary solvents {[( N , N -dimethylformamide) DMF, ( N , N -dimethylacetamide) DMAC, (dimethyl sulfoxide) DMSO and ( N -methylpyrrolidone) NMP] + ethyl acetate} was measured at various temperatures. The results revealed that the experimental solubility values of KA have positive correlations with mass fraction of co-solvents and temperature in investigated four binary solvents. The solubility order of KA with mass fraction of co-solvents being w 1 = 0.2000, 0.4000 and 0.6000 is: DMSO + ethyl acetate > NMP + ethyl acetate > DMAC+ ethyl acetate > DMF + ethyl acetate, respectively. However, in DMAC ( w 1 = 0.8005) + ethyl acetate, the solubility values of KA overtook that in NMP ( w 1 = 0.8004) + ethyl acetate at the higher temperature. The miscibility behaviors of KA with tested four binary solvents was revealed by the Hansen Solubility Parameters (HSPs). According to the calculated interaction energy, KA can be more easily dissolved in the solvent which has larger interaction energy with KA. Additionally, the measurement solubility data was mathematically correlated by the NRTL, Three-Suffix Margules, UNIQUAC models and Apelblat equation. The thermodynamic parameters (Δ dis G o , Δ dis H o , Δ dis S o , ζ H , ζ TS ) of KA dissolution processes in investigated four binary solvents were evaluated utilizing the van't Hoff equation. The positive Δ dis H o and Δ dis S o , indicate that the dissolution processes of KA are endothermic and entropy-driven in all chosen binary solvents. And the main contributor of Δ dis G o is positive enthalpy.
研究领域:
化学
·
物理化学
·
热力学
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材料科学
·
计算化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）