JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2019
·
IF 2.298
Yuwen Fan,Wenjun Zhu,Yonghong Hu,Wenge Yang,Qiang Xu,Xinxin Liu,Bin Heng
In the study, the solubility of l-serine were measured in eight common pure solvents and binary mixed solvents (acetonitrile + water, isopropanol + water, methanol + water, and acetone + water) from 278.15 to 333.15 K under atmospheric pressure by gravimetric method (acetone was measured from 278.15 to 328.15 K). The solubility of l-serine increases with increasing temperature, and the order in eight common pure solvents is water > acetonitrile > methanol > acetone > ethyl acetate > ethanol > n-butanol > isopropanol. In binary mixed solvents, the solubility of l-serine is dependent on temperature and solvent composition. Also, the solubility of l-serine increases with increasing temperature, and it decreases as the concentration of acetonitrile, isopropanol, methanol, or acetone increases at a constant temperature. The experimental data in eight pure solvents were fitted by the modified Apelblat model, Buchowski–Ksiazaczak λh model. The modified Apelblat equation, combined nearly ideal binary solvent/Redlich–Kister (CNIBS/R–K) model, and Jouyban–Acree model were used to correlate the experimental data in four binary solvents. Among the four models, the modified Apelblat model is more suitable with a higher accuracy than the other three models. The experimental data can be used to optimize the industrial manufacturing process of l-serine.
研究领域:
化学工程
·
生物化学
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产品关联
DOI
高影响因子
RSC Advances
·
2013
·Vol. 3
·期 48
·25982-25988
·
IF 2.562
Tiefan Huang,Mo Zhang,Lihua Cheng,Lin Zhang,Man Huang,Qiuping Xu,Huanlin Chen
A polysulfone (PSF)-based hollow fiber affinity membrane for in vitro endotoxin elimination was explored and prepared successfully. This depended on the well-known hemocompatibility of PSF membrane. The PSF hollow fiber membranes were firstly activated by a chloromethylation reaction, and treated with ethylenediamine and glutaraldehyde successively. L-Serine (Ser) was finally immobilized on the surface of the membranes as the functional ligand. The modified membrane was confirmed by X-ray photoelectron spectroscopy, attenuated total reflectance Fourier transform infrared spectroscopy, and contact angle analysis. L-Ser density up to 8.4 μmol cm−2 on PSF membranes could be achieved. The adsorption performance was preliminarily studied by comparing the PSF membrane and PSF–Ser membrane in deionized water and sodium phosphate buffer, showing that the PSF–Ser membrane had improved endotoxin adsorption capacity with decreased Bovine Serum Albumin (BSA) adsorption. The PSF–Ser membrane was further investigated in human plasma, and showed excellent removal efficiency and specificity for endotoxin. These results demonstrate the promising application potential of this novel affinity membrane in sepsis therapy.
研究领域:
生物医学工程
·
材料科学
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DOI
高影响因子
LETTERS IN APPLIED MICROBIOLOGY
·
2015
·Vol. 61
·期 4
·390-396
·
IF 1.659
Chao H.,Wu B.,Shen P.
Increase in L‐serine production is of interest for industry. Here, we describe a metabolic engineering approach to increase the production of L‐serine in a methylotrophic bacterium. mxaF, the gene encoding the large subunit of a methanol dehydrogenase, was cloned from Methylobacterium sp. MB200 through transposon mutagenesis. Deletion of mxaF gene prevented the strain to grow on methanol, suggesting that mxaF is involved in methanol metabolism. Overexpression of mxaF gene in the strain MB200 resulted in a fivefold increase in methanol dehydrogenase activity compared to the wild‐type. Resting cell assays showed that the recombinant strain accumulated 6·6 mg ml−1 L‐serine in 72 h with 30 mg ml−1 wet cells from 50 mg ml−1 glycine and 50 mg ml−1 methanol, representing a 1·5‐fold increment for L‐serine production in contrast to the wild‐type strain. These results demonstrate that the potential for improving the production of L‐serine can be achieved by overexpressing mxaF gene in methylotrophic bacteria.Significance and Impact of the StudyThe amount of L‐serine produced each year worldwide is relatively small compared with the amounts of the other amino acids and hence it is in great demand. Here, we describe a metabolic engineering approach to increase the production of L‐serine in a methylotrophic bacterium Methylobacterium sp. MB200. The result demonstrates that raising the output of L‐serine can be achieved by overexpressing mxaF gene in methylotrophic bacteria.
研究领域:
微生物学
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生物技术
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代谢工程
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DOI
PubMed
高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 6
·2855-2861
·
IF 1.821
Zhuo Ye,Lingxi Yang,Jianguo Wen,Baoxian Ye
A novel voltammetric sensor, based on a single-walled carbon nanotube (SWNT) composite poly(L-serine) film modified electrode, was fabricated by simple dipping–drying and electrodeposition methods and used for highly sensitive determination of natamycin. At this sensor, the electrochemical response of natamycin was improved greatly due to the synergistic effect of SWNTs and poly(L-serine). A new voltammetric method for the determination of natamycin was proposed with a detection linear range of 6 × 10−8 mol L−1 to 6 × 10−6 mol L−1 and a detection limit of 4 × 10−8 mol L−1. Furthermore, the electrode reaction of natamycin was investigated in detail and some dynamic parameters were calculated for the first time. Using the present method, the residual quantity of natamycin in available claret and beverage samples was evaluated with good satisfactory recovery.
研究领域:
分析化学
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食品科学
·
电化学
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DOI
高影响因子
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
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2023
·Vol. 332
·期 9
·3741-3752
·
IF 1.6
Yusheng Wang,Ying Dai,Wenmei Hu,Jinhua Xie,Qinqin Tao
Two Mg–Al layered bimetallic (hydrogen) oxides with uranium selective deposited space were obtained by intercalating bimetallic (hydrogen) oxides with L-serine with active amino and carboxyl sites, which showed excellent uranyl selectivity from cation mixture solutions of Ni 2+ , Co 2+ , Sr 2+ , Zn 2+ , La 3+ , Gd 3+ , Ce 3+ , and Sn 4+ . It was firstly proved in L-serine intercalated Mg–Al layered bimetallic (hydrogen) oxides. The key parameter, maximum uranium uptake amount, reached 1028.37 mg/g for LS-LDH and 1018.50 mg/g for MgAl-LDO/C at pH 4.5. These merits coupling with very high uptake capacity, highly cost effectivity, facile preparation and acceptable recycle performance makes LS-LDH and MgAl-LDO/C promising in the treatment of uranium-containing solutions. The study enables the understanding of selectively treating uranium-containing wastewater with L-serine intercalated Mg–Al layered bimetallic (hydrogen) oxides.
研究领域:
环境科学
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材料科学
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产品关联
DOI
高影响因子
Research
·
2023
·
IF 11
Nian Liu,Lihui Chen,Mingjie Yan,Qian Tao,Jie Wu,Jing Chen,Xiang Chen,Wei Zhang,Cong Peng
Natural killer (NK) cells, as key immune cells, play essential roles in tumor cell immune escape and immunotherapy. Accumulating evidence has demonstrated that the gut microbiota community affects the efficacy of anti-PD1 immunotherapy and that remodeling the gut microbiota is a promising strategy to enhance anti-PD1 immunotherapy responsiveness in advanced melanoma patients; however, the details of the mechanism remain elusive. In this study, we found that Eubacterium rectale was significantly enriched in melanoma patients who responded to anti-PD1 immunotherapy and that a high E. rectale abundance was related to longer survival in melanoma patients. Furthermore, administration of E. rectale remarkably improved the efficacy of anti-PD1 therapy and increased the overall survival of tumor-bearing mice; moreover, application of E. rectale led to a significant accumulation of NK cells in the tumor microenvironment. Interestingly, conditioned medium isolated from an E. rectale culture system dramatically enhanced NK cell function. Gas chromatography–mass spectrometry/ultrahigh performance liquid chromatography–tandem mass spectrometry-based metabolomic analysis showed that l-serine production was significantly decreased in the E. rectale group; moreover, administration of an l-serine synthesis inhibitor dramatically increased NK cell activation, which enhanced anti-PD1 immunotherapy effects. Mechanistically, supplementation with l-serine or application of an l-serine synthesis inhibitor affected NK cell activation through Fos/Fosl. In summary, our findings reveal the role of bacteria-modulated serine metabolic signaling in NK cell activation and provide a novel therapeutic strategy to improve the efficacy of anti-PD1 immunotherapy in melanoma.
研究领域:
免疫学
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肿瘤学
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微生物学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2025
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IF 5.2
Wei Lu,Hui Chen,Tingting Liu,JiaHao Hu,LuWen Zhu,XiaoHua Tao,XiaoLing Xu,YongZhong Du
Traditional wound care preparations frequently face challenges such as complex care protocols, poor patient compliance, limited skin permeability, lack of aesthetics, and inconvenience, in addition to the risk of bacterial infection. We developed a spray film preparation containing nanocellulose and L-serine modified nanosilver, capable of rapidly forming a transparent film on the skin within minutes of application. The incorporation of nanocellulose imparted protective, moisturizing, and breathable properties to the film, allowing for easy removal after use. Moreover, our innovative application of L-serine as a precursor for synthesizing silver nanoparticles not only overcomed the limitations of current silver nanoparticle synthesis methods but also enhanced antibacterial efficacy by leveraging the inherent antimicrobial properties and bacterial targeting capability of L-serine. Specifically, the addition of nanocellulose to the spray film forming system enhanced air permeability and drug carrying capacity by creating a lightweight porous structure, while also ensuring the antimicrobial and anti-inflammatory efficacy of serine modified silver nanoparticles. Mice treated with spray film formulations containing nanocellulose and silver nanoparticles demonstrated superior wound healing, normalization of keratin thickness, increased hair follicle count, and reduced inflammatory markers. In conclusion, our study developed a spray film that integrated excellent antimicrobial properties with user convenience. This research presented innovative strategies for clinical wound care, antibacterial infection management, and post laser cosmetic treatment, with the potential to substantially enhance wound care in practical application.
研究领域:
生物医学工程
·
药剂学
·
材料科学
·
感染与免疫
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产品关联
DOI
PubMed
高影响因子
INFECTION AND IMMUNITY
·
2019
·Vol. 87
·期 12
·
IF 3.16
Fang He,Zheng Yin,Chenlu Wu,Yaoyao Xia,Miaomiao Wu,Pan Li,Huihui Zhang,Yuanyuan Yin,Nengzhang Li,Guoqiang Zhu,Wenkai Ren,Yuanyi Peng
Pasteurella multocida causes a variety of infectious diseases in various species of mammals and birds, resulting in enormous economic loss to the modern livestock and poultry industry. However, the mechanism of host-pathogen interaction is unclear. Here, we found that l-serine levels were significantly decreased in murine lungs infected with P. multocida. Exogenous l-serine supplementation significantly increased the survival rate of mice and decreased the colonization of P. multocida in the lungs of mice. Notably, l-serine decreased the macrophage- and neutrophil-mediated inflammatory responses in mice during P. multocida infection.
研究领域:
微生物学
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免疫学
·
兽医学
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DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Liang Liu,Shiao Ren,Yangyi Hao,Lijuan Yue,Chenli Yue,Longyu Li,Wenlei Zhang,Zengliang Gao,Xin Hai
The aim of this study was to establish a simple, sensitive, and robust ultra-high performance liquid chromatography coupled with fluorescence detection method (UPLC-FLD) for the determination of chiral D/L-serine in cells. D/L-serine in cells were derivatizated by o-phthalaldehyde (OPA) and N-acetyl-L-cysteine (NAC). Carbocisteine was selected as the internal standard. The derivatives were separated on a C18 column by gradient elution. The excitation and emission wavelengths for fluorescence determination are 340 nm and 450 nm, respectively. The retention time of D-serine and L-serine was 23.3 and 23.9 min respectively, which presented a perfect separation. The accuracy of D-serine and L-serine were ranged from 96.46 % to 109.63 % and 95.50 %–102.20 %, respectively. The precision of D-serine and L-serine were ranged from 4.34 % to 14.56 % and 3.56 %–13.73 %. The limit of quantitation of D-serine and L-serine were 0.1 nmol/mL. The concentration of D/L-serine varies in different cell lines. This method can satisfy the determination of D/L-serine in astrocytes and other cells, which can be used for the study of D/L-serine metabolism and related mechanisms. In short, we have established a simple, stable, reliable and robust method for the determination of D/L-serine in cells. In addition, the D/L-serine levels in different cells were quantified in this study, which can provide a reference for the study of D/L-serine metabolism in cells.
研究领域:
分析化学
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生物化学
·
细胞生物学
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色谱学
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代谢研究
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xiangyan Xie,Xue Ao,Ruijuan Xu,Hongyi Lv,Shiqiao Tan,Jinrong Wu,Lijuan Zhao,Yi Wang
The combination of pharmacological and physical barrier therapy is a highly promising strategy for treating intrauterine adhesions (IUAs), but there lacks a suitable scaffold that integrates good injectability, proper mechanical stability and degradability, excellent biocompatibility, and non-toxic, non-rejection therapeutic agents. To address this, a novel injectable, degradable hydrogel composed of poly(ethylene glycol) diacrylate (PEGDA), sodium alginate (SA), and l -serine, and loaded with platelet-rich plasma (PRP) (referred to as PSL-PRP) is developed for treating IUAs. l -Serine induces rapid gelation within 1 min and enhances the mechanical properties of the hydrogel, while degradable SA provides the hydrogel with strength, toughness, and appropriate degradation capabilities. As a result, the hydrogel exhibits an excellent scaffold for sustained release of growth factors in PRP and serves as an effective physical barrier. In vivo testing using a rat model of IUAs demonstrates that in situ injection of the PSL-PRP hydrogel significantly reduces fibrosis and promotes endometrial regeneration, ultimately leading to fertility restoration. The combined advantages make the PSL-PRP hydrogel very promising in IUAs therapy and in preventing adhesions in other internal tissue wounds.
研究领域:
生物材料
·
组织工程
·
生殖医学
·
药剂学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Yirong Ma,Xinran Wei,Jiameng Xu,Shuhua Ji,Fan Yang,Aiguo Zeng,Yunzhe Li,Jiliang Cao,Jia Zhang,Zhimin Luo,Qiang Fu
Screening for illegal use of glucocorticoids (GCs) in cosmetics by electrochemical methods is extremely challenging due to the poor electrochemical activity of GCs. In this study, poly-L-Serine/poly-Taurine modified electrode (P(Tau)/P(L-Ser)/GCE) was prepared for sensitive and direct determination of betamethasone in cosmetics by a simple two-step in situ electropolymerization reaction. The relevant parameters of preparation and electroanalytical conditions were respectively studied, including the concentration of polymerization solution, the number of scanning circles and the scanning rate. The SEM and EDS mapping demonstrated successful preparation of P(Tau)/P(L-Ser)/GCE. The electro-catalytic properties of the obtained electrodes were investigated using cyclic voltammetry and differential pulse voltammetry methods, showing a remarkable improvement of sensitivity for the detection of betamethasone due to the synergic effect of both P(L-Ser) and P(Tau). In addition, we investigated the electrochemical reduction of betamethasone on the surface of modified electrode. It was found that the process was controlled by diffusion effect and involved the transfer of two electrons and two protons. Then the electrochemical sensor method based on P(Tau)/P(L-Ser)/GCE was established and delivered a linear response to betamethasone concentration from 0.5 to 20 μg mL −1 with a limit of detection of 32.2 ng mL −1 , with excellent recoveries (98.1%–106.8%) and relative standard deviations (＜4.8%). Furthermore, the established electrochemical sensor method was compared with conventional HPLC method. The results showed that both of them were comparable. Moreover, the established electrochemical sensor method was with the merits of short analysis time, environmentally friendly, low cost and easy to achieve in-site detection.
研究领域:
分析化学
·
电化学
·
化妆品科学
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DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2014
·
IF 4.195
Ziming Zhao,Yu Wang,Jin Han,Keli Wang,Dan Yang,Yihua Yang,Qian Du,Yuanjian Song,Xiaoxing Yin
The aim of this work was to design, synthesize, and characterize self-assembled micelles based on polypeptides as a potential antitumor drug carrier. Amphiphilic poly( l -phenylalanine)- b -poly( l -serine) (PFS) polypeptides were obtained through the polymerization of N -carboxyanhydride. As a novel hydrophilic segment, poly( l -serine) was utilized to enhance tumor targeting due to a large demand of tumors for serine. PFS could self-assemble into micelles with an average diameter of 110–240 nm and a slightly negative charge. PFS polypeptides adopted random coil in pH 7.4 phosphate-buffered saline and could partly transform to α-helix induced by trifluoroethanol. PFS micelles with a low critical micelle concentration of 4.0 μg mL −1 were stable in pH 5–9 buffers and serum albumin solution. PFS micelles had a loading capacity of 3.8% for coumarin-6 and exhibited a sustained drug release. Coumarin-6 loaded rhodamine B isothiocyanate-labeled PFS micelles were incubated with Huh-7 tumor cells to study the correlation between drugs and carriers during endocytosis. The uptake of drugs was consistent with the micelles, illustrating that the intracellular transport of drugs highly depended on the micelles. PFS micelles diffused in whole cytoplasm while coumarin-6 assumed localized distribution, suggesting that the micelles could release the loaded drugs in particular areas. The internalization mechanism of PFS micelles was involved with clathrin-mediated endocytosis and macropinocytosis. Excess serine inhibited the uptake of PFS micelles, which demonstrated that serine receptors played a positive role in the internalization of PFS. The more interesting thing was that the uptake inhibition impacted on normal cells but not on tumor cells at the physiological concentration of serine. The difference in the uptake of PFS micelles was fourfold as high between the tumor cells and the normal cells, which indicated that PFS micelles had good tumor targeting in vitro. In conclusion, PFS micelles reported in this work were a promising drug delivery system for tumor targeting therapy.
研究领域:
药学
·
生物医学工程
·
纳米技术
·
癌症研究
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）