ANALYTICAL CHEMISTRY
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2024
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IF 6.7
Lingyu Nie,Hui Zhang,Weiheng Kong,Rong-Mei Kong,En-Sheng Zhang,Jin Li,Yan Zhao,Fengli Qu
Designing artificial mimetic enzymes with high activity/selectivity to replace chiral bioenzymes is of great interest in the development of chiral materials consisting of molecules, enantiomers, that exist in two forms as mirror images of one another but cannot be superimposed. In this study, the chiral catalytic structural unit was streamlined from tyrosinase to integrate a mimetic nanozyme. The chiral amino acid l-histidine, as the chiral binding/recognition site, and the active metal site Cu were coupled (Cu@l-His) to create a copper–histidine brace with enantioselective catalytic ability to tyrosinol enantiomers. Results of kinetic parameters and activation energies confirmed the excellent peroxidase-like activity with a preference of Cu@l-His to l-tyrosinol. Such a preference could be attributed to the structurally oriented copper–histidine brace with a stronger affinity and catalytic activity to l-tyrosinol. By accurately evaluating chiral recognition units derived from bioenzymes, stable and superior chiral mimetic nanoenzymes could be constructed in a more straightforward and simplified manner, and they could also be extended to the reconstruction of diverse chiral enzymes.
研究领域:
生物化学
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纳米技术
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催化
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手性材料
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DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 10
·2167
·
IF 4.2
Yuxia Li,Min Li,Liuzhi Hu,Baozhu Zhang
In this study, Cu2+modulated silver nanoclusters were constructed for the turn-on, label-free detection of L-histidine. Six Ag NCs protected by oligonucleotides (DNA-Ag NCs) were tested in a series of experiments. Finally, A-DAN-Ag NCs were chosen as the best candidate due to their excellent fluorescent properties. The fluorescence of A-DAN-Ag NCs was quenched using Cu2+through energy or electron transfer. However, quenched fluorescence could be restored dramatically in the presence of L-histidine due to Cu2+liberation from A-DAN-Ag NCs and because of the chelation between the imidazole group of L-histidine and Cu2+. The proposed sensor exhibited high selectivity towards L-histidine over other amino acids, with a limit of detection (LOD) of 0.096 μM ranging from 0 to 8 μM. The proposed sensor succeeded in detecting L-histidine in diluted human urine. Therefore, the sensor has promising practical applications in biological systems.
研究领域:
生物化学
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分析化学
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生物医学
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PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2019
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IF 3.206
Qiqi Tan,Juan Qiao,Rongyue Zhang,Li Qi
Papaya juice protected copper nanoclusters was depicted for detection of serum l -histidine. The fluorescent probe was synthesized by using a simple “one pot” hydrothermal preparation strategy with papaya juice playing dual roles both as a green reductant and stabilizer. The excitation and emission peaks of the copper nanoclusters were at 400 nm and 490 nm, respectively. The fluorescence was turned-on in addition of l -histidine due to the enhanced surface electron density. The fluorescent intensity increased linearly with l -histidine concentration ranging from 2.0 to 50.0 µM. The proposed copper nanoclusters was further applied to determine l -histidine in rat serum after intraperitoneal injection.
研究领域:
生物化学
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分析化学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
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IF 4.831
Ruihong Yao,Zhijian Li,Panpan Huo,Congcong Gong,Gang Liu,Chunhong Zheng,Shouzhi Pu
In this study, we constructed zinc based metal–organic framework functionalized by L-histidine (His@ZiF-8). The His@ZiF-8 had high porosity, large surface area, abundant carboxyl and amino group, which has been found to greatly enhance the aggregation-induced emission (AIE) of tetracycline (TC). After the His@ZiF-8 enrichment with TC, the TC exhibited strong fluorescence emission peak at 565 nm based on AIE. Under the optimal conditions, the fluorescence intensity of TC exhibited a good linear relationship with TC concentration within 0.1–80 μM with a low limit of detection of 28.6 nM. Antibiotic analogs such as neomycin, chloramphenico, ampicillin, kanamycin, and erythromycin have no obvious interference. In addition, the developed method was successfully used to the detection of TC in milk, river water, and honey.
研究领域:
材料科学
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分析化学
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环境科学
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DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
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2014
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IF 3.05
Cunliu Zhou,Huiqiong Ye,Tadayuki Nishiumi,Hao Qin,Conggui Chen
The objective of this investigation was to evaluate the effects of l -histidine on the stability of porcine hemoglobin concentrates during storage. The results indicated that the addition of l -histidine increased the oxyhemoglobin content and a* values but decreased the methemoglobin content and free iron content (P < 0.05) during storage. The addition of iron cation decreased both the oxyhemoglobin content and a* values but increased both the methemoglobin content and free iron concentration (P < 0.05). CD spectra revealed that the free iron content induced the transformation of the hemoglobin secondary structure from an α-helix (close structure) to a β-pleated, β-turn, and random coil arrangement (loose structure), while l -histidine weakened this behavior of the free iron. Infrared spectra demonstrated that the l -histidine coordinated with the free iron (Fe 2 + or Fe 3 + ) to give the corresponding complex at 25 °C and pH 7.3. Therefore, l -histidine enhanced the stability of hemoglobin concentrates by coordinating with free iron.
研究领域:
生物化学
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医学工程
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DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2025
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IF 4.8
Ye Yuan,Yuan Liu,Zhipeng Shen,Huidan Wu,Lantian Meng,Xiaoxiao Guo,Bing Jiang,Ling Fang
Background: Bacterial resistance remains a significant challenge, necessitating the development of new antibacterial strategies. This study introduces a rapidly synthesized L-histidine- Fe3O4 (L-His-Fe3O4) nanozyme with enhanced peroxidase (POD)-like activity, designed to improve antibacterial efficacy and accelerate the healing of bacteria-infected wounds.Methods: We successfully synthesized L-His-Fe3O4 using an ultrafast, room-temperature synthesis method, and observed its anti-infection effect and explored its anti-infection mechanism through in vivo and in vitro antibacterial experiments.Results: We produced L-His-Fe3O4 cost-effectively while preserving L-His, which was essential for its catalytic and antibacterial functions. The resulting nanozyme demonstrated exceptional antibacterial activity against both Gram-positive (S. aureus) and Gram-negative (E. coli) bacteria. In vivo experiments revealed that L-His-Fe3O4 outperformed vancomycin in reducing bacterial viability and effectively promoting wound healing, all while maintaining excellent biosafety with no adverse effects on blood or liver functions.Discussion: These findings highlight the potential of L-His-Fe3O4 for large-scale production and practical use in treating bacterial infections, offering a promising approach to combating antibiotic-resistant pathogens.
研究领域:
生物材料学
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微生物学
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药理学
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PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
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IF 5.4
Miao Yuan,Saidan Wang,Hongxia Du,Yating Sui,Xiangjing Zhang,Song Zhao,Qiushi Li,Shaojie Liu,Xuteng Xing,Erjun Tang
Two-dimensional Ti 3 C 2 T x MXene, with a large specific surface area, high chemical stability and excellent barrier properties, has become a promising of anti-corrosion filler in organic coatings. However, the poor dispersion and compatibility in organic coatings restrict its efficient application. To this end, L-histidine (L-His) corrosion inhibitor was grafted on the 3-aminopropyl triethoxysilane (APTES) functionalized Ti 3 C 2 T x MXene surface, under the electrostatic attraction between the carboxylate and protonated aminopropyl groups. The obtained APTES-Ti 3 C 2 T x MXene@L-His cannot only accelerate the dispersion of Ti 3 C 2 T x MXene filler, but also greatly promote the anti-corrosion property and service lifetime of coatings through combining the physical shielding effect of MXene laminate structure with active anti-corrosion function of L-His corrosion inhibitor. Results show that amino-functionalized Ti 3 C 2 T x MXene can maintain homogeneous in epoxy resin for 300 h, enhancing its dispersion in epoxy coating. Moreover, APTES-Ti 3 C 2 T x MXene@L-His composite can achieve the continuous release of L-His for 3000 min, and additional acid-base dual response property, the releasing rate of L-His is significantly accelerated in acidic or alkaline solutions. The total loading amount of L-His in APTES-Ti 3 C 2 T x MXene@L-His composite is about 3 %. Furthermore, the practical composite coating containing APTES-Ti 3 C 2 T x MXene@L-His also exhibits the excellent anti-corrosion and certain self-healing properties, such as its |Z 0.01 Hz| value remains above 10 10 Ω·cm 2 during the 40 d immersing in 3.5 NaCl solution, which is 3 orders of magnitude higher than that of pure epoxy coating. This work extends the great significance for Ti 3 C 2 T x MXene based composite coatings application of in the field of anti-corrosion.
研究领域:
材料科学
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腐蚀与防护
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有机涂层改性
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DOI
高影响因子
Analytical Methods
·
2014
·Vol. 7
·期 2
·684-689
·
IF 1.938
Yuan Su,Li Qi,Xiaoyu Mu,Minglin Wang
In this work, L-histidine-stabilized gold nanoclusters were prepared by a green one-pot process and employed as a selective probe for sensing ferric ions. The obtained probe exhibited bluish green fluorescence, and exhibited an excitation maximum at 394 nm and emission maximum at 492 nm. The fluorescence of the probe was obviously quenched by the introduction of ferric ions, hinting that the synthesized probe could be applied for sensing ferric ions. Further, the selectivity of the proposed probe for sensing ferric ions was investigated. The results indicated that the fluorescent probe had favorable sensitivity and selectivity for the detection of ferric ions. Moreover, this probe was further used for the analysis of ferric ions in bean sprouts. It showed that the gold nanocluster based fluorescent probe has great potential for sensing metal ions in plants.
研究领域:
分析化学
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植物学
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DOI
高影响因子
JOURNAL OF PHYSICAL CHEMISTRY B
·
2021
·
IF 2.991
Bin-Cheng Wang,Xiu-Ping Li,Biao-Biao Hao,Chen-Xi Zhang,Qing-Lun Wang
A coordination polymer with dual functions of high proton conductivity and highly sensitive fluorescent sensors demonstrates a great application potential. In this work, a cadmium-based coordination polymer (denoted as CP 1) with hydrothermal stability was synthesized. The abundant coordination water, lattice water, and amino groups make an extended hydrogen-bonding pathway for efficient proton migration, which endows CP 1 with the highest proton conductivity of 2.41 × 10–3 S·cm–1 at 353 K and 98% RH. Especially, the proton conductivity of the chitosan (CS) hybrid membrane containing CP 1 reaches a maximum value of 2.62 × 10–2 S·cm–1 under 343 K and 98% RH, which increases almost 7 times higher than that of the pure CS membrane due to the host–guest collaboration. Furthermore, luminescence studies revealed that CP 1 is a high-sensitivity and good-selectivity fluorescent probe for the detection of trace amounts of l-histidine with a lowest detection limit of 1.0 × 10–8 M.
研究领域:
材料科学
·
化学
·
生物传感
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DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Yuanyuan Li,Qianqian Du,Xiaodan Zhang,Yuming Huang
Tetracycline (TC), a widely-used antibiotic to treat bacterial infections, combines with Eu 3+ to form a stable EuTC complex that has a low fluorescence (FL) yield because of Eu 3+ coordination with water molecules. We report a simple, label-free ratiometric fluorescent platform for sensitively and selectively sensing of TC, using L-histidine caped gold nanoclusters (His-AuNCs) as a FL indicator and an enhancer of Eu 3+ FL. The His-AuNCs were prepared via chemical reduction of Au(III) by L-histidine, which was a reducer and a protecting agent. The His-AuNCs exhibited good photostability, outstanding stability toward high ionic strengths , storage stability, and favorable optical properties . In the absence of TC, the AuNCs-Eu 3+ system displays strong FL emission at 475 nm ( F 475 ) from the His-AuNCs and weak FL at 620 nm from Eu 3+ ( F 620 ) with excitation at 375 nm. TC quenches the His-AuNC FL and greatly enhances the Eu 3+ emission. This is attributed to an enhancement of the EuTC complex fluorescence by the His-AuNCs. Thus, a ratiometric F 620 / F 475 FL signal can be used for TC detection by simply mixing AuNCs and Eu 3+ . Under the optimized conditions, the linear range from 10 nM to 60 μM and a detection limit of 4 nM (S/N = 3) for TC were obtained. The application of the assay platform for the detection of TC in environmental and biological samples was demonstrated. The sensing platform has advantages of easy preparation, rapid response, high sensitivity, and good selectivity.
研究领域:
分析化学
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环境科学
·
生物医学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2019
·
IF 4.916
Yongmei Wang,Zongshan Zhao,Jianyuan Qin,Huan Liu,Aifeng Liu,Mengmeng Xu
A simple, green and nondestructive method based on terahertz fingerprint peaks has been developed for rapid in situ analysis of l -histidine and α-lactose in dietary supplements. Fingerprint absorption peaks of l -histidine and α-lactose located at 0.77 and 0.53 THz could be directly used for identification and quantitation of these analytes in commercial dietary supplements. Compared with the partial least squares regression model (PLSR), the linear least squares regression (LLSR) method based on peak areas presented better performance, with the linear correlation coefficients of 0.9899 and 0.9910 for l -histidine and α-lactose, respectively. Furthermore, analysis time per sample can be shortened to less than 1 min due to the narrower spectral acquisition region. The accuracies were 94.8–110% and 98.9–110%, comparable to those of ion chromatography for l -histidine and high-performance liquid chromatography for α-lactose. The results presented great potential of the developed method for rapid in situ analysis of nutrients in dietary supplements.
研究领域:
分析化学
·
营养学
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DOI
PubMed
高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 15
·6133-6138
·
IF 1.821
Li-Qiang Yan,Yun Ma,Mei-Fang Cui,Zheng-Jian Qi
A coumarin-based “Turn-on” fluorescence chemosensor 1 containing L-histidine for Al3+ was readily synthesized and investigated, which showed high selectivity and remarkable sensitivity to aluminium(III) ions based on a photo-induced electron-transfer (PET) mechanism in aqueous solution (Tris–HCl, 0.1 mM, pH 7.2). The sensor responded rapidly to Al3+ in aqueous solutions with a 1 : 1 stoichiometry. Meanwhile, it showed excellent adaptability and also responsiveness.
研究领域:
分析化学
·
生物化学
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DOI
高影响因子
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