MONATSHEFTE FUR CHEMIE
·
2019
·Vol. 150
·期 7
·1349-1354
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IF 1.501
Li Yunhao,Liu Shiquan
Abstract A porous silica gel with both COOH and NH 2 groups was synthesized in one pot by the addition of l -glutamic acid ( l -GA) into a synthesis medium consisting of tetraethyl orthosilicate, water, ethanol, and 1-butylamine. The inclusion of l -GA in the as-synthesized product ( l -GA-SiO 2 ) was evidenced by FT-IR and TG measurements. The l -GA was chemically bonded with silica as indicated by the shift of FT-IR bands at 1510 and 1400 cm −1 in the spectrum of l -GA to higher wavenumbers of 1540 and 1460 cm −1 in the one of l -GA-SiO 2 . The TG analysis showed that the l -GA-SiO 2 had an increase of weight loss by 5.6% due to the decomposition of the included l -GA which was centered at around 313 °C, suggested by a new peak in the DTG curve. Compared with the one synthesized without l -GA, the l -GA-SiO 2 was more aggregated with smaller surface area. However, the latter showed a higher removal efficiency by 17.1% toward zinc ions in water than the former and the adsorbed ions were hardly released again into water from the used adsorbent. Graphical abstract
研究领域:
材料科学
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环境科学
·
化学工程
✓ 已验证
DOI
高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
·
2024
·Vol. 42
·期 5
·570-578
·
IF 4.1
Sun Xiao-Fei,Zhang Xu,Song Shu-Ping,Yao Ya-Qun,Zhang Yan,Wang Cheng-Liang,Sun Jing-Jiang,Wang Qing-Fu
L -glutamic acid (LA) is a bio-based, non-toxic, environmentally friendly material derived from biomass. The present study reports the application of Passerini three-component polymerization (P-3CP) for the straightforward preparation of LA-based light-responsive polyesters (PLTDs) under mild conditions. PLTDs with molar masses up to 8500 g/mol and high yields exceeding 90% are obtained. The chemical structures and light-responsive self-immolative behavior of PLTDs are comprehensively characterized by employing ultraviolet-visible (UV-Vis) spectroscopy, size exclusion chromatography (SEC), nuclear magnetic resonance (NMR) spectroscopy, and liquid chromatography mass spectrometry (LC-MS). Meanwhile, monodisperse PLTD-based doxorubicin-loaded nanoparticles (PLTD-DOX-NP) (size=193 nm, PDI=0.018) are formulated by nanoprecipitation method. Upon light-induced depolymerization, the PLTD-DOX-NP undergoes rapid decomposition, resulting in a burst release of 80% cargo within 13 s. Furthermore, according to biological toxicity tests, the PLTD-NP possesses adequate biosafety, both before and after irradiation. Overall, the incorporation of P-3CP with biorenewable LA-based monomer adheres to the principles of green chemistry, significantly simplifying the synthetic pathway of light-responsive polymers.
研究领域:
材料科学
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生物医学工程
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绿色化学
✓ 已验证
DOI
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 51
·45829-45834
·
IF 3.289
Xia Lin,Qinghong Wang,Shu Zhu,Juanjuan Xu,Qiao Xia,Yingzi Fu
In this article, a sensitive and stereo-selective biosensor for L-glutamic acid (L-Glu) based on the electrochemiluminescence (ECL) of Ru(bpy)32+ has been designed by applying L-glutamic dehydrogenase (GLDH) for enzymatic generation of NADH in situ. The novel ECL Glu biosensor was prepared by immobilizing GLDH on the modified electrodes with Ru(bpy)32+–platinum nanoparticles (Ru–PtNPs) and reduced graphene oxide–multiwall carbon nanotubes–Nafion (rGO–MWCNT–Nf). Fourier transform infrared spectroscopy (FT-IR), electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM) and cyclic voltammetry (CV) were employed to investigate the characteristics of different materials. In addition, the ECL technology was adopted to explore the interactions between the prepared Glu biosensor and L- or D-Glu. A remarkable difference of ECL intensity was observed when the proposed biosensor interacted with L- and D-Glu, respectively, and good responses were obtained from L-Glu in a range from 1.0 × 10−7 M to 5.0 × 10−3 M with a low detection limit of 3.3 × 10−8 M (S/N = 3). The developed ECL biosensor showed satisfying sensitivity, selectivity, reproducibility and stability, therefore, it might provide a new perspective for the recognition and detection of L-Glu.
研究领域:
生物化学
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分析化学
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DOI
高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
·
2013
·Vol. 31
·期 2
·201-210
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IF 1.27
Zhang Shusheng,Chen Chongyi,Li Zhibo
We investigated the ring opening polymerization (ROP) of di- and tri-ethylene glycol monomethyl ether functionalized L-glutamate N -carboxyanhydrides (NCAs) using hexamethyldisilazane (HMDS) as primary initiator and 1,5,7-triazabicyclo-[4.4.0]dec-5-ene (TBD) as co-initiator. The binary initiator system afforded a living ROP for these pegylated NCAs, and a series of homopolypeptides with controlled molecular weight (MW) and low polydispersity were obtained. We then systematically studied the helical content and clouding point (CP) dependence on polypeptide MW using circular dichroism (CD) spectroscopy and turbidity measurements, respectively. We found that the helical content of both homopolypeptides increased with MW, but the triethylene glycol functionalized poly-L-glutamate (poly-L-EG 3 Glu) intended to form more stable α -helical structure than diethylene glycol functionalized counterpart (poly-L-EG 2 Glu) at similar MW. Accordingly, the CP of poly-L-EG 2 Glu with known end group has strong dependence on its helical content, which is essentially determined by MW. Our results suggested that the thermal responsive properties of these unique pegylated poly-L-glutamates not only rely on their chemical structure but also on their secondary structures, which is different from conventional thermal responsive polymers.
研究领域:
高分子化学
·
生物材料学
✓ 已验证
DOI
高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2018
·Vol. 18
·期 1
·576-582
·
IF 1.354
Yu Yadong,Wu Na,Peng Gang,Li Tao,Jiang Ling,Huang He
Convenient and sensitive methods for the detection of α-ketoglutarate (α-KG) will be beneficial for biomedical diagnosis and microbial fermentation industry. In this work, reduced MoS2 nanoparticles (rMNs) were prepared by ultrasonication and electrochemical reduction.
The rMNs were then decorated with gold nanoparticles (Aunano) to fabricate rMNs-Aunano nanocomposite. The glass carbon electrode modified with rMNs-Aunano nanocomposite (rMNs-Aunano-GCE) showed excellent electro-catalytic oxidation toward NADH with
a satisfied reproducibility and exhibited linear response in the range of 38.46–214.29 μM. Moreover, the L-Glutamic dehydrogenase modified rMNs-Aunano-GCE exhibited a sensitive and fast response to α-KG, with a linear response range of 11.12–52.94 μM
and a detection limit of 6.25 μM. These findings will pave ways for the α-KG detection and the application of MoS2 or other two-dimensional materials in electrochemical sensing.
研究领域:
生物医学
·
微生物发酵工业
·
电化学传感
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DOI
高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2014
·
IF 7.261
Haiyang Yu,Zhaohui Tang,Dawei Zhang,Wantong Song,Ying Zhang,Yan Yang,Zaheer Ahmad,Xuesi Chen
Platinum-based polymeric nano-drugs, especially cisplatin-loaded polymeric nanoparticles (CDDP-NPs), have been extensively exploited for the treatment of solid tumors . However, it is still unclear what role the processing procedure and the properties of the polymeric carrier materials may play in influencing the plasma pharmacokinetics , biodistribution and in vivo efficacy of CDDP-NPs. In this study, a series of poly( l -glutamic acid)- g -methoxy poly(ethylene glycol) (PLG- g -mPEG) copolymers were synthesized for the preparation of CDDP-loaded PLG- g -mPEG (CDDP/PLG- g -mPEG) nanoparticles . All of the parameters, including PLG molecular weight, mPEG/PLG weight ratio, mPEG chain length, ultrafiltration purification and cisplatin loading content, were found to have a significant influence on the plasma pharmacokinetics of the CDDP/PLG- g -mPEG nanoparticles . The blood circulation time of the nanoparticles was prolonged with increases in PLG molecular weight, mPEG/PLG weight ratio, mPEG chain length and CDDP loading content. The use of ultrafiltration purification could prolong the blood circulation time of the nanoparticles as well. Experiments to measure the pharmacokinetics and biodistribution demonstrated that the selected CDDP/PLG- g -mPEG nanoparticles, NP10, had a long blood circulation time and could achieve selective and significant accumulation in Lewis lung carcinoma (LLC) tumors. The platinum plasma concentrations in the LLC tumor-bearing mice receiving NP10 remained up to 46-fold higher than that of mice receiving equivalent doses of free CDDP. In addition, the plasma area under the concentration time curve (AUC) of NP10 was 31-fold higher than that of free CDDP in 48 h. The platinum concentration ratio of NP10 to free CDDP in tumors reached as high as 9.4. The tumor AUC ratio of NP10 to CDDP was 6. Using a mouse C26 tumor model, here we demonstrate that NP10 improves the safety and tolerance in vivo when compared to CDDP and effectively inhibits the growth of C26 tumors. Furthermore, increasing the dosage of NP10 by 2 or 3-fold of free CCDP improved its anticancer efficacy to comparable or higher levels. These results indicate that CDDP/PLG- g -mPEG nanoparticles have greater potential for the treatment of solid tumors in clinical application.
研究领域:
药理学
·
肿瘤学
·
纳米医学
✓ 已验证
DOI
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 28
·17005-17019
·
IF 3.108
Shifeng Yan,Taotao Wang,Xing Li,Yuhang Jian,Kunxi Zhang,Guifei Li,Jingbo Yin
Injectable hydrogels as an important biomaterial class have been widely used in regenerative medicine. A series of injectable poly(L-glutamic acid)/chitosan (PLGA/CS) hydrogels were fabricated by self-crosslinking aldehyde-modified PLGA (PLGA–CHO) and lactic acid-modified chitosan (CS–LA). The oxidation degree of PLGA–CHO and degree of substitution (DS) of CS–LA could be adjusted by the amount of sodium periodate and lactic acid, respectively. The effect of the solid content of the hydrogels, oxidation degree of PLGA–CHO and CS–LA/PLGA–CHO mass ratio on the gelation time, gel content, water uptake, mechanical properties, microscopic morphology, and in vitro degradation of the hydrogels was examined. The pH and ion sensitivity of PLGA/CS hydrogels was also examined. Encapsulation of rabbit chondrocytes within the hydrogels showed viability of the entrapped cells and cytocompatibility of the injectable hydrogels. The injectable PLGA/CS hydrogels demonstrated attractive properties for future application in pharmaceutical delivery and tissue engineering.
研究领域:
生物材料
·
再生医学
·
高分子科学
·
生物医学工程
·
细胞生物学
✓ 已验证
DOI
高影响因子
Frontiers in Neuroscience
·
2024
·
IF 3.2
Hongxiong Zhang,Taixiang Gao,Zhao,Nan Wang,Zhixuan Li,Xuemei Qin,Ying Liu,Rui Wang
Insomnia and depression are psychiatric disorders linked to substantial health burdens. The gut microbiome and metabolomic pathways are increasingly recognized as key contributors to these conditions’ pathophysiology. Suanzaoren Decoction (SZRD), a traditional Chinese herbal formulation, has demonstrated significant therapeutic benefits for both insomnia and depression. This study aims to elucidate the mechanistic effects of SZRD on insomnia and depression by integrating gut microbiome and metabolomic analyses and to assess the differential impacts of SZRD dosages. Using ultra-high-performance liquid chromatography-mass spectrometry (UHPLC–MS), we identified 66 chemical constituents within SZRD. Behavioral assays indicated that low-dose SZRD (LSZRD) significantly ameliorated insomnia symptoms in rat models, whereas high-dose SZRD (HSZRD) markedly improved depressive behaviors. 16S rRNA sequencing revealed that SZRD modulated gut microbiome dysbiosis induced by insomnia and depression, characterized by an increased abundance of short-chain fatty acid (SCFA)-producing genera. Metabolomic profiling demonstrated reduced plasma amino acid metabolites and disrupted γ-aminobutyric acid (GABA) and L-glutamic acid metabolism in the hippocampus of affected rats. SZRD administration restored fecal SCFA levels and ameliorated metabolic imbalances in both plasma and hippocampal tissues. These findings underscore the pivotal role of gut microbiome modulation and metabolic regulation in the therapeutic effects of SZRD, providing a scientific basis for its use in treating insomnia and depression.
研究领域:
药理学
·
神经科学
·
微生物学
·
代谢组学
·
中医
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF POLYMER SCIENCE
·
2021
·Vol. 59
·期 24
·3111-3121
·
IF 0
Xu Zhang,Wei Zhao,Chengliang Wang,Lan Cao,Qingfu Wang,Jingjiang Sun
In this work, a series of polyesters with different functional side groups were successfully prepared by Passerini three-component polymerization (P-3CP) in “one-pot” from biorenewable l -glutamic acid ( l -GA)-based monomers. First, the polymerization conditions including monomer feed ratios, solvents and comonomer were systematically studied using Boc-protected l -GA with adipaldehyde and tert -butyl isocyanide. Under the optimal polymerization conditions, a variety of N -substituted l -GA-based monomers as well as functional isocyanides were attempted in the P-3CP to produce polyesters with different functionalities. Moreover, it was found that after removal of pendant protecting groups, the obtained l -GA-based polyester degraded spontaneously into small molecules via 1,5-intramolecular cyclization between the pendant amine groups and the ester groups in polymer backbone. The structures and thermal properties of obtained polymers were determined by 1 H NMR, IR, SEC, MALDI-ToF-MS, DSC, and TGA measurements. Starting from biorenewable l -GA, this approach will provide a facile and straightforward route to produce functional and biodegradable polyesters, thus effectively expanding the range of biodegradable polymers. Graphical
研究领域:
材料科学
·
高分子化学
·
生物降解高分子
·
可持续化学
✓ 已验证
DOI
危险品化学品经营许可证（带存储）