ECS Journal of Solid State Science and Technology
·
2024
·Vol. 13
·期 12
·124004
·
IF 1.8
Deng Pan,Gaoyuan Ren,Jingwei Zhang,Li Wang,Shudong Wang
Addition of corrosion inhibitor in the slurry during tungsten (W) chemical mechanical planarization (CMP) process is an effective way to avoid excessive corrosion. In this work, three environmentally friendly amino acids (lysine, leucine, and glutamic acid) were chosen as corrosion inhibitor during W CMP process. A smooth wafer surface was obtained after CMP with the addition of lysine. The static etching rate of the W film decreased from 45.9 Å min−1 to 7.1 Å min−1 while an excellent wafer surface roughness (Ra = 1.267 nm) was obtained with the addition of 0.25 mmol l−1 lysine. The results indicate that increasing the amount of protonated amino acid molecules in the slurry can result in the improvement of corrosion inhibition performance as well as decrease the material removal rate. Electrochemical tests indicated that the corrosion inhibition effect can be attributed to the formation of an amino acids adsorption layer on the wafer surface, inhibiting the formation of peroxotungstic acids by the interaction between H2O2 and W film in the concave region. This work provides valuable guidance for the application of environmentally friendly amino acid corrosion inhibitor in the CMP field.
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 411
·期 27
·7177-7185
·
IF 3.286
Yi Zhengjun,Wang Shuhui,Meng Xiangying,Wu Anqi,Li Qian,Song Yongjie,Zhao Ronglan,Qiao Jinjuan
Accurate and rapid identification of Staphylococcus aureus ( S. aureus ) is of great significance for controlling the food poisoning and infectious diseases caused by S. aureus . In this study, a novel strategy that combines lysin cell-binding domain (CBD)-based magnetic separation with fluorescence detection was developed for the specific and sensitive quantification of S. aureus in authentic samples. The S. aureus cells were separated from the sample matrix by lysin CBD-functionalized magnetic beads. Following lysis by lysostaphin, intracellular catalase was released from S. aureus cells and detected by a fluorometric system composed of horseradish peroxidase (HRP), hydrogen peroxide (H 2 O 2 ), and Amplex Red. S. aureus was quantified via the inhibitory effect of the released intracellular catalase on the fluorometric system since the catalase could decompose the H 2 O 2 . Optimized conditions afforded a calibration curve for S. aureus ranging from 1.0 × 10 2 to 1.0 × 10 7 CFU mL −1 . The detection limit was as low as 78 CFU mL −1 in phosphate-buffered saline (PBS), and the total detection process could be completed in less than 50 min. Other bacteria associated with common food-borne and nosocomial infections negligibly interfered with S. aureus detection, except for Staphylococcus epidermidis , which may have slightly interfered. Moreover, the potential of this proposed method for practical applications has been demonstrated by detection assays of sterilized milk and human serum.
Graphical abstract
研究领域:
微生物学
·
分析化学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2021
·
IF 3.196
Yujing Bian,Xun Zhang,Zhenqi Zhu,Bin Yang
To date, it is challengeable to clearly identify and accurately characterize molecular vibrational modes of an amino acid and its hydrate. In this work, the terahertz absorption spectra of L -Lysine (Lys) and L -Lysine hydrate (Lys·H 2 O) have been measured by terahertz time-domain spectroscopy (THz-TDS) at the range of 0.3–2.5 THz under room temperature. The structural changes of Lys and Lys·H 2 O are sensitively observed by THz spectroscopy. Based on density functional theory (DFT), the vibrational frequencies of Lys and Lys·H 2 O are analyzed, the Lys single molecule model and the most optimal Lys·H 2 O single molecule model are established. The collective vibrational modes of Lys and Lys·H 2 O have been identified by potential energy distribution (PED) analysis and molecular vibrational animation of GaussView. The results demonstrate that the collective vibrational modes of Lys and Lys·H 2 O at various frequencies are originated from the dihedral torsion or the bond angle bend of different molecular chains in a single molecule, which drives the vibrations of related groups. Therefore, the combination of THz-TDS technology and PED analysis opens up an opportunity for identifying Lys and its hydrate, which provides a theoretical reference for studying vibrational modes of biomolecules.
研究领域:
生物物理学
·
光谱学
·
理论化学
✓ 已验证
DOI
高影响因子
Microorganisms
·
2024
·Vol. 12
·期 1
·179
·
IF 4.1
Jiajia Wu,Tianyu Chu,Jian Hao,Liang Lin
Sortase-mediated ligation (SML) is widely used for protein bioconjugation. However, the sortase used in this strategy typically recognizes only the N-terminal oligoglycine, which is absent in most natural proteins. To broaden the spectrum of substrates compatible with SML, we focus on a novel sortase, sortase A fromStreptococcus pneumoniae(SpSrtA), known for its expanded substrate specificity (N-terminal glycine, alanine, and serine). We present the first evidence showing that the reported SpSrtA mutant (SpSrtA*) can modify lysine residues in itself and other proteins. The modification sites of SpSrtA* were identified through LC-MS/MS analysis. Moreover, we discovered an optimal lysine-containing peptide tag by fusing it onto sfGFP, resulting in a labeling efficiency of 57%. Inspired by this, we applied the method to modify proteins on microorganism surfaces up to 13.5-fold. To enhance labeling efficiency, we fused the SpSrtA* onto a surface protein and achieved a 2.64-fold improvement. We further developed a high-throughput yeast display screening method for the directed evolution of SpSrtA*, achieving a 10-fold improvement in the labeling efficiency of this surface protein. Our study provides a novel strategy for modifying the lysine residues that will be a powerful addition to the protein bioconjugation toolbox.Keywords:isopeptide ligation;sortase A;yeast display
研究领域:
生物化学
·
分子生物学
·
蛋白质工程
·
生物技术
✓ 已验证
DOI
PubMed
高影响因子
MATERIALS SCIENCE FORUM
·
2015
·
IF 0
Feng Ye,Guo Dong Tang,Si Chen,Bo Zhen Wu,Meng Ma,Yan Qin Shi,Xu Wang
L-lysine-based dendritic gelator(G3-C12-G3) was successfully designed and synthesized using a divergent approach and it can self-assemble into nanofibers in MMA followed by gelation. After thermal polymerization of MMA gels, the self-assembly nanofibers modified PMMA nanocomposite can be obtained. SEM studies indicate the embedded supra molecular nanofibres joint each other and form a relative intact gel networkskeleton in PMMA matrix. Impact strength and DMTAmeasurementindicatethe existence of gel network skeleton make PMMA nanocomposite had higher impact strength and storage modulus than pure PMMA. UV-vis spectrophotometer studies indicate PMMA nano-composite retains high light transmittance.
研究领域:
材料科学
·
高分子化学
·
纳米技术
✓ 已验证
DOI
STAR Protocols
·
2025
·
IF 1.3
Yu Ran,Qiqing Yang,Lianqi Zhou,Heyu Li,Bing Yang,Long Zhang
Summary L-lactate has been recognized as an essential molecule for signaling and metabolic balance. Lactylation, a post-translational modification (PTM) derived from L-lactate, is commonly observed on various proteins and plays essential roles in cellular processes. Here, we present a protocol to globally profile the lactylation proteome and perform label-free quantification. We provide steps for cell preparation, protein extraction, digestion, peptide desalting, and lactylpeptide enrichment. Additionally, we outline the parameters for liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. For complete details on the use and execution of this protocol, please refer to Li et al. 1
研究领域:
生物化学
·
蛋白质组学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Crystals
·
2025
·Vol. 15
·期 9
·752
·
IF 2.4
Haifeng Luo,Yuchen Zheng,Shizhao Ren,Wangchuan Xiao,Rongrong Xue,Wei Han,Fenghua Chen
Andrographolide (ADG) is a typical poorly water-soluble drug, and a co-amorphous strategy was used here to improve its aqueous solubility. Co-amorphous systems of ADG and amino acids with a 1:1 molar ratio were screened via the neat ball milling method. L-lysine (Lys) and L-tryptophan (Trp) can be used as co-formers with ADG, forming a co-amorphous phase, which was confirmed by powder X-ray diffraction, IR and Raman spectroscopy. ADG-Trp showed poor solubility at 37 °C, which was close to that of raw ADG (0.08 mg·mL−1). ADG-Lys showed unexpectedly enhanced solubility, at 0.5 mg·mL−1in the media of water and PBS (pH 7.4) and 0.3 mg·mL−1in the medium of HCl buffer (pH 1.2) at 37 °C. ADG-Lys showed good storage stability for 5 months, but its thermal stability was poor and it could recrystallize at 100 °C. Compared with ADG-Trp, ADG-Lys has weaker hydrogen bonding interactions and stronger hydrophobic interactions related to ADG molecules, which might cause the unusual enhancement in solubility. To our knowledge, ADG-Lys prepared in this work shows the maximum ADG content (70 wt.%) and the highest ADG solubility among the reported ADG amorphous solid dispersions and co-amorphous systems.
研究领域:
药剂学
·
材料科学
·
药物递送
·
物理化学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2012
·Vol. 4
·期 9
·2711-2714
·
IF 1.547
Yanli Zhou,Zhichong Yang,Maotian Xu
A simple, reliable and sensitive colorimetric method for the detection of lysine was developed based on the use of citrate-stabilized gold nanoparticles (AuNPs). In acid media (pH = 3.0), AuNPs exhibited a sensitive color change from red to blue after the addition of lysine for 30 min at room temperature, which could directly be seen with the naked eye and monitored by UV-vis absorption spectroscopy. Results showed that the absorbance ratio (A680/A520) was linear for a lysine concentration in the range of 1 to 70 μM (r = 0.995), and the limit of detection was 0.8 μM at a signal-to-noise ratio of 3. This method also demonstrated excellent selectivity for lysine over other amino acids. For the analysis of lysine in food samples, precision and accuracy were checked.
研究领域:
分析化学
·
生物化学
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Kai Hao,Ronglu Cui,Lifeng Fang,Jun Ling,Baoku Zhu
Polysarcosine (PSar) is an electrically neutral and excellently hydrophilic polypeptoid showing limited interaction with proteins and cells, which possesses better biocompatibility compared with polyethylene glycol. However, the immobilization of PSar is difficult due to the high water solubility. Herein, lysine-sarcosine PiPo, which was the random copolymer of lysine and sarcosine (PLS), was synthesized via a phosgene-free and water-tolerable polymerization of N-phenyloxycarbonyl-amino acids for the first time. PLS was immobilized by tannic acid (TA) on the polysulfone (PSf) membrane for a short time to obtain a neutral surface. The modified membrane showed improved hydrophilicity, decreased protein adsorption, and low cytotoxicity. Moreover, barely any hemolysis, no platelet adhesion, prolonged clotting time, and low complement activation further suggested good hemocompatibility. In order to improve the antifouling ability of the membrane under pressure, the neutral surface was oxidized by sodium periodate, which accelerated the chemical reaction between amino groups in PLS and phenolic hydroxyl groups in TA. Meanwhile, carboxyl groups generated due to the decomposition of TA and a negatively charged surface were obtained. While maintaining the good properties of the unoxidized one, the hydrophilicity of the oxidized membrane was improved and the clotting time was further prolonged. Besides, the filtration recovery of the oxidized membrane was improved remarkably. This approach of rapid immobilization of PSar has great potential for applications in the biomedical area, especially for blood-contacting materials.
研究领域:
生物医学工程
·
材料科学
·
生物材料
·
血液相容性
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Xin Xu,Tian Xue,Qingqing Jiang,Daming Fan,Mingfu Wang,Yueliang Zhao
The inhibitory effects of four hydrocolloids, including alginic acid, pectin, carboxymethylcellulose sodium salt, and chitosan on the formation of two typical toxic advanced glycation end products N Ɛ -(carboxymethyl) lysine (CML) and N Ɛ -(carboxyethyl) lysine (CEL) were investigated in chemical models and fish patties. All the four hydrocolloids were found to significantly inhibit CML and CEL formation in chemical models, and among them, pectin and alginic acid were the most effective inhibitors. In roast fish patties, the addition of pectin and alginic acid at 0.9% reduced the content of CML by 43.6% and 44.9%, and CEL by 32.4% and 32.6%, respectively. Moreover, alginic acid plus a polyphenol epigallocatechin gallate (EGCG) showed stronger inhibitory effect against CML and CEL formation than alginic acid alone. Further study showed that alginic acid and pectin decreased the formation of glyoxal (GO) and methylglyoxal (MGO), two key intermediates of CML and CEL, in the chemical models and fish patties. In addition, compared with EGCG, the addition of alginic acid in fish patties showed less impact on color, odor, and sensory properties. These findings suggest that alginic acid is a potent inhibitor against the formation of AGEs with less impact on food quality.
研究领域:
食品科学
·
化学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
iScience
·
2022
·
IF 6.107
Dawei Yang,Jie Yin,Liuqun Shan,Xingling Yi,Wei Zhang,Yongbin Ding
Summary Cancer cells tend to utilize aerobic glycolysis to generate energy and metabolites; the end product of aerobic glycolysis is lactate, which promotes lysine lactylation (Kla). Kla is a newly discovered histone post-translational modification (PTM) that plays important roles in regulating gene expression. However, Kla in non-histone mammalian proteins is unclear. Here, a comprehensive analysis of lactylated proteins in gastric cancer AGS cells was conducted. There were 2375 Kla sites found in 1014 proteins. Interestingly, KEGG pathway analysis showed that these proteins were significantly enriched in spliceosome function. In addition, Kla was more abundant in gastric tumors than in adjacent tissues, and high levels of Kla in gastric tumors were associated with poor prognosis. These results suggest that Kla could be a prognostic marker in gastric cancer. This lysine lactylome analysis in gastric cancer cells, the first of its kind, provides a valuable foundation for further studies of Kla.
研究领域:
癌症生物学
·
蛋白质组学
·
分子生物学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
ENGINEERING IN LIFE SCIENCES
·
2015
·Vol. 16
·期 3
·299-305
·
IF 2.485
Naiqiang Li,Howard Chou,Yan Xu
Cadaverine has the potential to become an important platform chemical for the production of nylon. Previously, a system that overexpresses the Klebsiella oxytoca lysine decarboxylase in Escherichia coli was engineered. The system was optimized by codon optimization, and tuning the expression level of the gene by testing various promoters and inducer concentrations. Here, we further improved the system by optimizing the sequence located in the region of the ribosome-binding site in order to enhance translation efficiency. We also identified mutant lysine decarboxylase enzymes that demonstrated enhanced cadaverine-production ability. Together, these modifications increased cadaverine production in the system by 50%, and the system has a yield of 80% from lysine-HCl under the conditions we tested. This is the first time that a system to produce cadaverine using the lysine decarboxylase from K. oxytoca performed at a level that is competitive with the traditional systems using the E. coli lysine decarboxylases in both lab-scale and batch fermentation conditions.
研究领域:
生物技术
·
代谢工程
·
合成生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）