Sensing and Bio-Sensing Research
·
2024
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IF 5.4
Weiran Mao,Xiaoyan Zhang,Yuanyuan Yin,Xiaohua Tang,Qingqing Jiang,Xia Chen,Xiaoliang Chen
This study proposes a novel sandwich-based ratio electrochemiluminescence (ECL) immunosensor for detecting monocyte chemotactic protein-1 (MCP-1), a marker of cardiovascular disease. We synthesized sulfur-doped palladium nanosheets (S-PdNS), which possess a stable structure and uniform morphology, and can quench signals generated by the S 2 O 8 2− /O 2 ECL system. Using glutaraldehyde (GA), we conjugated luminol to these nanosheets and combined them with a secondary antibody. This modification enabled the nanomaterial to produce a new luminol signal as an internal reference for detection while quenching the S 2 O 8 2− /O 2 ECL signal. To date, no studies have utilized both mechanisms for detection. Furthermore, the deposition of gold nanoparticles (AuNPs) on the surface of the glassy carbon electrode (GCE) allowed for increased antibody binding and improved electron transmission in the ECL system. When trace analytes are present, the S 2 O 8 2− /O 2 ECL signal decreases, and the luminol ECL signal increases. This setup allowed us to obtain two signals from one measurement and use the ratio to construct a standard curve, significantly reducing the possibility of misjudgment. Under optimal conditions, the linear detection range for MCP-1 is 10 –2.5 pg mL −1 to 10 3 pg mL −1 , with a detection limit of 1.6 fg mL −1 (S / N = 3). The immunosensor also exhibited excellent selectivity, repeatability, and stability, expanding the application of the dual luminescence mechanism and providing an effective method for early monitoring of cardiovascular disease.
研究领域:
生物医学工程
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分析化学
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DOI
高影响因子
SCIENTIA HORTICULTURAE
·
2018
·
IF 1.76
Fujuan Wei,Jin Wang,Shuai Huang,Bi Gong
Lilies ( Lilium spp.) are in demand worldwide because of their superior commercial and ornamental value. To develop new and practical preservation technologies, effects of pre-harvest treatment of Promalin (a.i. 1.8% each of 6-benzyladenine (6-BA) and gibberellic acid (GA 4 + 7 )) and 1-Methylcyclopropene (1-MCP) on cut lily under cold storage were investigated. Oriental lily ‘Sorbonne’ was selected for this study. The field plants at coloring stage were sprayed with 25 mg L −1 Promalin, 30 μL L −1 1-MCP additionally, their combination (1-MCP + Promalin) was also tested. Deionized water was used as control. The flower stems were harvested 24 h later and stored at 3 ± 1 °C. Preservation effect and physiological changes, e.g. inflorescence life, flower life, opening rate, dry weight loss rate, respiration rate, ethylene production rate, malondialdehyde (MDA) content, lipoxygenase (LOX) activity, energy metabolism related indexes including adenosine triphosphate (ATP) content, energy charge, activities of ATP synthase (ATPase), succinate dehydrogenase (SDH), cytochrome oxidase (CCO) and Ca 2+ -ATPase were determined. The results showed that 1-MCP or Promalin treatment extended the vase life of stored cut lilies and inhibited the decline of the dry weight of petals, reduced respiration rate, ethylene production rate, LOX activity and the accumulation of MDA. They also maintained the activity of ATPase, Ca 2+ -ATPase, SDH, CCO, ATP content and energy charge of cut lilies at a higher level than control. 1-MCP + Promalin exhibited an additive effect. Combination treatment maintained the flower opening ability as high as 89.3% after five-week storage, control merely maintained it till 3 weeks. These data indicate growth regulators (e.g. 1-MCP or Promalin) can extend the storage life of cut lily ‘Sorbonne’ through cutting down respiration consumption and manipulation of energy balance.
研究领域:
植物生理学
·
园艺学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Weijie Luo,Mutong Wu,Xuan Zhao,Zhongshan Zhang,Haifang Yang,Hua Cai,Quan Yu
A miniature photoionization toroidal ion trap (TorIT) mass spectrometer prototype was developed for the analysis of volatile organic compounds (VOCs) in ambient air. The TorIT analyzer features a simplified configuration comprising two planar and two ring electrodes, enabling efficient ion trapping and mass analysis in a compact structure. A microchannel plate (MCP) detector was strategically used to capture ions radially ejected from the trap, with its geometry well-matched to the TorIT design. These two modules were integrated into a compact assembly with an overall length of 50 mm. The entire system exhibits excellent pressure tolerance, allowing for stable operation at elevated pressures up to 0.11 Pa. To address background noise from MCP operation under high pressure and UV scattering, we implemented a discontinuous ionization strategy coupled with optimized signal filtering, significantly improving the quality of the ion peaks. During direct atmospheric sampling through a 75 μm capillary, the PI-TorIT instrument achieves sensitive VOC detection with a demonstrated limit of detection of 483 ppbv for anisole. The successful integration of the TorIT analyzer and MCP detector may present a viable approach for developing compact mass spectrometers.
研究领域:
分析化学
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质谱分析
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环境监测
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仪器与探测器开发
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产品关联
DOI
高影响因子
LIFE SCIENCES
·
2024
·
IF 5.2
Chao Sun,Qiushuang Ma,Liya Feng,Jianbo Ji,Dandan Du,Pengfei Shang,Xiuli Guo
Aims This study aims to investigate the role of SOD3 in gastric cancer (GC) progression and its impact on chemotherapy efficacy and toxicity. It further seeks to evaluate the therapeutic potential of MCP in enhancing SOD3 activity to improve treatment outcomes and reduce chemotherapy-induced peripheral neurotoxicity (CIPN). Materials and methods We used overexpression plasmids and small interfering RNAs (siRNAs) to modulate the expression of SOD3 and Desmocollin2 (DSC2) in gastric cancer cells. Molecular biology experiments were performed to analyze pathway-related protein expression and molecular interactions. In vitro and in vivo experiments were conducted to evaluate the effects of modified citrus pectin (MCP) and oxaliplatin (OXA), individually and in combination, on gastric cancer progression and CIPN. Key findings SOD3 inhibited the proliferation, migration, and invasion of GC cells via SOD3/EGFR/PKP3/DSC2 axis. MCP selectively increased SOD3 levels and enhanced its anti-tumor effects. Combined treatment with MCP and OXA synergistically inhibited GC progression in vitro and in vivo, while MCP alleviated CIPN, enabling OXA dose reduction without compromising efficacy. Significance The findings revealed that SOD3 played a critical tumor-suppressive role in gastric cancer by modulating the SOD3/EGFR/PKP3/DSC2 axis. MCP, a natural compound that selectively boosted SOD3 levels, enhanced chemotherapy efficacy while reducing peripheral neurotoxicity, providing a promising strategy to improve gastric cancer treatment and mitigate chemotherapy-related side effects.
研究领域:
肿瘤学
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分子生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
Nano Research
·
2024
·
IF 9.5
Zhu Shengjie,Huang Danqing,Luan Qichen,Li Yang,Gan Jingjing,Zhao Yuanjin,Sun Lingyun
Macrophages play a pivotal role in systemic lupus erythematosus (SLE) therapy. Efforts have been made to develop multifunctional drug delivery systems capable of directing macrophage polarization. Here, we present a novel hyaluronic acid methacrylate (HAMA) hydrogel microparticle encapsulating multiple cytokines for SLE remission though enhancing macrophage functions. The HAMA microparticles loaded with monocyte chemotactic protein-1 (MCP-1) and interleukin-4 (IL-4) were fabricated by using a microfluidic technology. The released MCP-1 facilitates the aggregation of inflammatory macrophages, after which IL-4 induces the macrophage phenotype shift from inflammatory M1 to immune-protective M2, thus restoring immune balance. We have demonstrated in MRL/lpr mice that the hydrogel microparticles could improve their efficacy of intraperitoneal drug delivery, modulate immune function, and attenuate the disease symptoms. These results suggest that our proposed microparticles delivery platform has potential clinical value for treating autoimmune diseases.
研究领域:
药理学
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免疫学
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生物医学工程
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药物递送系统
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自身免疫疾病
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Weiran Mao,Junlin He,Zhiyong Tang,Chengli Zhang,Jun Chen,Jia Li,Chao Yu
A novel electrochemical immunosensor was proposed for the detection of monocyte chemoattractant protein-1 (MCP-1), a biomarker of cardiovascular disease. Due to thionine (Thi) possessing electroactive redox properties, a one-step approach was utilized to synthesize a reduced graphene oxide-tetraethylene-thionine-Au (rGO-TEPA-Thi-Au) nanocomposite at room temperature using the synergistic effect of Thi and rGO-TEPA towards HAuCl 4 . We obtained the excellent matrix material , which immobilized more primary antibody MCP-1-Ab 1 on rGO-TEPA on a modified glassy carbon electrode (GCE). To further enhance the sensitivity of the sensor, a novel signal generation and amplification strategy was developed for detection. RuPdPt trimetallic nanoalloy particles (RuPdPt TNPs), a novel nanomaterial , were synthesized by a one-pot method, displayed a uniform morphology as well as good electrochemical activity and bound with the secondary antibodies against MCP-1 via the Pt-NH 2 bond. Most importantly, RuPdPt TNPs have a significant ability to catalyze H 2 O 2 to produce an electron. The electrochemical signal was highly amplified because the electrochemical signal was primarily derived from the synergistic catalysis of H 2 O 2 by RuPdPt TNPs and recorded by chronoamperometry . Under the optimal conditions, this newly designed biosensor exhibited sensitive detection of MCP-1 in the range from 20 fg mL −1 to 1000 pg mL −1 , with a detection limit of 8.9 fg mL −1 (based on a S/N = 3). Additionally, the designed immunosensor showed acceptable selectivity , reproducibility and stability. This immunosensor is a promising strategy for analyzing clinical serum samples in the future.
研究领域:
生物化学
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纳米技术
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电化学
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生物传感器
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医学诊断
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产品关联
DOI
PubMed
高影响因子
ACS Biomaterials Science & Engineering
·
2023
·
IF 5.8
Can Cheng,Heng Li,Jingwen Liu,Liang Wu,Zhengdong Fang,Geliang Xu
Tissue engineering approaches such as the electrospinning technique can fabricate nanofibrous scaffolds which are widely used for small-diameter vascular grafting. However, foreign body reaction (FBR) and lack of endothelial coverage are still the main cause of graft failure after the implantation of nanofibrous scaffolds. Macrophage-targeting therapeutic strategies have the potential to address these issues. Here, we fabricate a monocyte chemotactic protein-1 (MCP-1)-loaded coaxial fibrous film with poly(l-lactide-co-ε-caprolactone) (PLCL/MCP-1). The PLCL/MCP-1 fibrous film can polarize macrophages toward anti-inflammatory M2 macrophages through the sustained release of MCP-1. Meanwhile, these specific functional polarization macrophages can mitigate FBR and promote angiogenesis during the remodeling of implanted fibrous films. These studies indicate that MCP-1-loaded PLCL fibers have a higher potential to modulate macrophage polarity, which provides a new strategy for small-diameter vascular graft designing.
研究领域:
组织工程
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生物材料
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免疫学
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血管生物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Zhou-Wei Wu,Xing-Rong Peng,Xiao-Cui Liu,Luan Wen,Xin-Yu Tao,Abdulbaset Al-Romaima,Ming-Yi Wu,Ming-Hua Qiu
Lepidium meyenii Walp., also known as the “Peruvian national treasure”, is a popular functional food in the daily lives of Peruvian people due to its bioactive with main polysaccharides. However, studies on polysaccharides isolated from Lepidium meyenii were few. Two new highly heterogeneous polysaccharides, MCP-1a and MCP-2b, were isolated and purified from the tuber of Lepidium meyenii . The structure characterization revealed that MCP-1a primarily consisted of D-Glc and had a molecular weight of 6.6 kDa. Its backbone was composed of 1,4,6- α -D-Glc, while branches feature T- α -L-Ara, 1,5- α -L-Ara, and T- α -D-Glc attached to the O -6 positions. MCP-2b was a rare arabinogalactan with a molecular weight of 49.4 kDa. Interestingly, the backbone of MCP-2b was composed of 1,6- β -D-Gal, 1,3,6- β -D-Gal with a few 1,3- β -D-Glc p A-4-OMe units inserted. Side chains of MCP-2b were mainly composed of 1,3- β -D-Gal, T- β -D-Gal, T- α -L-Ara, 1,5- α -L-Ara, with trace amounts of 1,4- β -D-Glc and T- β -D-Glc. The bioactivity assay results revealed that MCP-1a and MCP-2b increased the release of NO, IL-1 β , TNF- α , and IL-6 from RAW 264.7 cells at concentrations ranging from 50 μg/mL to 400 μg/mL. Furthermore, MCP-1a and MCP-2b could promote the expression of key transcription factors (IκB- α , p-IκB- α , p65, and p-p65) in the NF-κB pathway, indicating that MCP-1a and MCP-2b had potential immunomodulatory activities.
研究领域:
药理学
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免疫学
·
植物化学
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DOI
PubMed
高影响因子
GREEN CHEMISTRY
·
2017
·Vol. 19
·期 21
·5103-5113
·
IF 9.125
Ying Duan,Min Zheng,Dongmi Li,Dongsheng Deng,Lu-Fang Ma,Yanliang Yang
The catalytic conversion of HMF to 2-hydroxy-3-methyl-2-cyclopenten-1-one (MCP), which is a valuable edible essence that has traditionally been obtained from adipic acid, was achieved with an isolated yield of 58%. This procedure comprised two steps: the hydrogenation of 5-hydroxymethylfurfural (HMF) to 1-hydroxy-2,5-hexanedione (HHD) in water on Pd/Nb2O5 catalysts and then the isomerization of HHD to MCP in the presence of a base. The Nb2O5 supports, which were acidic, were characterized by FTIR, XRD and NH3-TPD. The supported Pd/Nb2O5 catalysts, in which Pd was highly dispersed, were synthesized employing cyclohexene as a reductant and were characterized by XRD, TEM, ICP-AES, XPS, EDX and CO pulse chemisorption. The high conversion of HMF was attributed to the high dispersion of Pd, and the acidity of the supports led to high selectivity for HHD. The conversion of HHD to MCP was an intramolecular aldol condensation reaction, and the protonic solvent favored this reaction. Ca–Al was proved to be an effective solid base for the conversion of HHD to MCP in water.
研究领域:
化学工程
·
催化化学
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Xiaoqin Wu,Zhiyi Shan,Lufan Wang,Wenwei Shi,Yanyan Li,Hongfang Cai,Liang Zhou,Chen Huan
Anthocyanins are bioactive substances that are not only responsible for the pigmentation of various fruits, but also hold potential benefits to human health. In this study, postharvest peaches were treated with 10 ppm 1-MCP, and changes of anthocyanin biosynthesis were then revealed at transcriptional level to gain insight into the underlying molecular mechanisms. The functions of key transcription factors (TFs) were systematically analyzed. Results demonstrated that 1-MCP effectively up-regulated expression of structural genes and stimulated anthocyanin accumulation. A novel transcription factor PpbHLH135 can respond positively to 1-MCP treatment and has been noted for its remarkable gene expression and protein abundance. The transcription factor bHLH3 was also investigated in this study due to its widespread interest and attention, serving to compare the regulatory function of bHLH135 in anthocyanin synthesis, although it didn’t show a significant response to 1-MCP treatment in this study. PpbHLH3 TF showed a stronger regulatory effect on PpF3H , while PpbHLH135 was more dominant over the regulation of PpUFGT . Future genetic and molecular experimentation will elucidate whether PpbHLH135 TF, in combination with other TFs, can play a stronger regulating role in fruit pigmentation, or in other metabolic pathways.
研究领域:
植物学
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分子生物学
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DOI
高影响因子
Foods
·
2022
·Vol. 11
·期 19
·2978
·
IF 5.561
Wenyan Lin,Yaping Liu,Jianbing Di,Gang Ren,Wei Wang,Weichun He,Yu Wang
Root mustard is plentiful in vitamins and minerals but shrivels and molds easily. In this study, freshly harvested root mustard was fumigated with various concentrations of 1-Methycyclopropene(1-MCP) (1 µL L−1, 1.5 µL L−1, and 2.0 µL L−1) for 24 h and stored at ambient temperature (17 ± 1 °C) for 35 d. Our data showed that 1-MCP fumigation had a striking preservation effect on maintaining weight loss, fruit firmness, lignin, Vc content, and moisture content, inhibiting respiratory intensity and ethylene release rate, as well as decreasing cell permeability and malondialdehyde (MDA) accumulation and maintaining cell membrane integrity of root mustard. In addition, lipoxygenase (LOX), pyruvate dehydrogenase (PDH), and polyphenol oxidase (PPO) activities were significantly reduced throughout the storage period. In contrast, the activities of succinate dehydrogenase (SDH), superoxide dismutase (SOD), ascorbate peroxidase (APX), phenylalanine deaminase (PAL), and peroxidase (POD) remained at high levels. Results showed that 1-MCP treatments were effective in maintaining the quality of root mustard, and the preservation effect of 1.0 μL·L−11-MCP was better than other concentrations of 1-MCP. This study could serve as a theoretical reference for root mustard preservation.Keywords:1-MCP;root mustard;physiology;storage quality
研究领域:
植物科学
·
食品科学
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采后生理学
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DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Jiarui Tong,Xiangrong Zhu,Xiumei Chen,Nengguo Tao
Chinese bayberry is a highly fragile and perishable fruit that requires protective and functional packaging to maintain its postharvest quality. In this study, a novel bio-based aerogel was developed using a gelatin/dialdehyde starch matrix reinforced with cellulose nanofibrils (CNF) via Schiff-base crosslinking and freeze-drying. The as-prepared aerogel exhibited superior compressibility, elasticity, and biodegradability. This aerogel was further functionalized by loading trans-2-hexenal (E2H) and combined with 1-methylcyclopropene (1-MCP) treatment, thereby constructing a postharvest preservation system for Chinese bayberry with the triple functionality of ethylene inhibition, antifungal activity, and cushioning performance. The optimal aerogel formulation (30% CNF content) demonstrated robust mechanical performance and sustained E2H release behavior, which followed first-order kinetics. In vitro and in vivo assays confirmed its significant inhibitory effect against Penicillium citrinum , the primary postharvest pathogen of bayberry. Storage experiments at 4 °C revealed that the synergistic effect of E2H-loaded aerogel pads and 1-MCP treatment effectively reduced decay, preserved firmness and nutritional quality, and extended the shelf life of bayberries to 18 d, significantly surpassing the control group which lost commercial value after 8 d. Furthermore, the aerogel completely degraded in soil within two weeks, confirming its potential as an eco-friendly packaging material. This work, integrating both protective and active preservation functionalities, provides a sustainable and efficient packaging approach for fragile fruit.
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）