Shiwei Xu,Guofeng Wang,Yueyue Feng,Liying Huang,Zhongyu Yang,Yu Zhang,Jiacheng Dong,Yajun Wang,Nannan Liu
Gastric cancer (GC) is one of the leading causes of cancer-related mortality worldwide. miRNA-106a is a small non-coding RNA that is significantly up-regulated in GC cells. CD44 protein serves as a cell adhesion molecule in GC, and its high expression is associated with tumor invasiveness and metastatic potential. Detection of these two biomarkers for GC can significantly improve the sensitivity and specificity of tests, thus identifying patients more accurately and facilitating early diagnosis and intervention. In this study, we proposed a bottom-up assembly method of silica nanowires (SiNWs) modified glass microtube to construct a glass microsensor based on the functionalization of SiNWs-dual probes (SiNWs@DGM), which can be used for the simultaneous detection of the two gastric cancer markers mentioned above in a highly sensitive and specific manner. The detection limit of the sensor is as low as 1 aM for the target miRNA-106a and 1 pg/mL for CD44 protein. The constructed SiNWs@DGM demonstrates excellent selectivity, high anti-interference ability, specificity, and good storage stability in sample analysis without any amplification. Therefore, our biosensing strategy can reduce false positive and false negative results, providing more reliable support for gastric cancer management and ultimately improving patient prognosis and survival rates.
Determination of microRNAs (miRNAs) as valuable blood-borne biomarkers has attracted many scientific attentions. However, analytical methods are still restricted by miRNAs intrinsic characteristics. In this study, for the first time, novel blackberry-like magnetic DNA/FMMA nanospheres were synthesized and mounted on a gold stir-bar as signal amplification probes. To produce this strong electrochemical signal label, double strand DNAs were immobilized on gold coated magnetic nanospheres through a hybridization chain reaction followed by reversible addition-fragmentation chain-transfer polymerization, which brought a great quantity of the electroactive tags (FMMA) on the nanosphere surface. These nanospheres were then fixed on the gold stir-bar as signal probes. The magnetic DNA/FMMA nanosphere probes can be released by substituting with the newly emerging DNA fragments of catalyzed hairpin assembly products. Eventually, these signal probes were magnetically enriched on the electrode surface to produce electrochemical signal and finally, the biosensor was developed to detect miRNA-106a (model target). The suggested aptamer-based biosensor demonstrated considerable selectivity, acceptable storage stability, high specificity, and excellent performance in real sample analysis without any pretreatments. As a result, current study reveals that the developed strategy has a great potential for the early diagnosis of gastric cancer and additionally the clinical monitoring of any miRNA sequences.
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