BIOSENSORS & BIOELECTRONICS
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2024
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IF 10.7
Haomin Fu,Zhiyuan Xu,Zhou Yang,Jianping Lei
High excitation potential is recognized as a harmful factor for the biological activity of biomacromolecules, such as proteins and nucleic acids, in electrochemiluminescence (ECL) biosensing. Developing low-potential ECL luminophores is vital for improving ECL accuracy in actual sample sensing. In this work, based on porous metal-organic framework (MOF) structure with multiple active sites and energy transfer between the excited ligands and Ln nodes, we designed a series of Ln-MOFs and observed ECL emission at low potential, providing a novel method to realize low-potential ECL. The MOF nanoemitters were prepared using 1,3,5-tri (4-carboxyphenyl)benzene ligand and several lanthanide ions as nodes through mild hydrothermal reaction. Interestingly, strong ECL emission at +0.75 V of peak potential was observed in the ECL-potential curve of Tb-based MOF using 2,2′,2″-nitrilotriethanol as coreactant, which was beneficial for reducing background interference in biosensing, and this ECL emission was attributed to the energy transfer between Tb and excited ligand. This low-potential ECL was then applied to construct an ECL biosensor with newly developed Cas12a-based method for selective detection of microRNA-155 without the help of strand displacement or reverse transcription. For this ECL system, the limit of detection was 0.78 nM, and the overall detection time was 2.5 h. The Ln-MOF nanoemitter provides a robust ECL platform to selectively detect various targets by integrating new bio-related techniques.
研究领域:
生物传感
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分析化学
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分子生物学
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DOI
PubMed
高影响因子
MOLECULES
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2020
·Vol. 25
·期 5
·1022
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IF 3.267
Yue Zhang,Yifang Xie,Leifang Zhang,Hang Zhao
Smoke inhalation causes acute lung injury (ALI), a severe clinical disease with high mortality. Accumulating evidence indicates that microRNA-155 (miR-155) and suppressor of cytokine signaling 1 (SOCS-1), as mediators of inflammatory response, are involved in the pathogenesis of ALI. In this paper, we explored the proinflammatory mechanism of miR-155 in smoke-inhalation-induced ALI. Our data revealed that smoke inhalation induces miR-155 expression, and miR-155 knockout (KO) significantly ameliorates smoke-inhalation-induced lung injury in mice. Neutrophil infiltration and myeloperoxidase (MPO), macrophage inflammatory protein 2 (MIP-2) and keratinocyte chemoattractant (KC) expressions were decreased in miR-155–/–mice after smoke inhalation as well. Real-time RT-PCR and immunoblotting results showed that SOCS-1 level was remarkably increased in miR-155–/–mice after smoke exposure. Furthermore, the experiments performed in isolated miR-155 KO pulmonary neutrophils demonstrated that the lack of SOCS-1 enhanced inflammatory cytokines (MIP-2 and KC) secretion in response to smoke stimulation. In conclusion, smoke induces increased expression of miR-155, and miR-155 is involved in inflammatory response to smoke-inhalation-induced lung injury by inhibiting the expression of SOCS-1.Keywords:acute lung injury;neutrophils;microRNA-155;suppressor of cytokine signaling-1
研究领域:
呼吸病学
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免疫学
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分子生物学
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PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
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2019
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IF 3.298
Hui Li,Guilan Di,Yi Zhang,Rongrong Xue,Jing Zhang,Junping Liang
Cadmium (Cd) is a nonessential metal that is a contaminant in aquatic ecosystems . Cd can accumulate in aquatic animals, leading to detrimental effects in tissues, and Cd exposure can induce immunotoxicity in fish. MicroRNAs (miRNAs) play critical roles in immune responses, yet the participation of miRNAs in Cd-induced immunotoxicity remains poorly understood. The present study evaluated the effects of Cd exposure on the immune responses and the mRNAs and miRNAs expressions of immune-related genes in Cyprinus carpio ( C. carpio ). Then, microRNA-155 (miR-155) was overexpressed and microRNA-181a (miR-181a) was knocked down to determine which miRNA plays a key role in the immune response to Cd. The results showed that 0.5 mg/L Cd 2+ significantly decreased the activity of alkaline phosphatase (AKP) and acid phosphatase (ACP) in the kidneys of C. carpio . Cd exposure upregulated the mRNA expressions of interleukin (IL)-1β, IL-8, nuclear factor-kappa B (NF-κB), tumour necrosis factor-α (TNF-α), and Toll-like receptor 4(TLR-4) and downregulated those of IL-10 and heme oxygenase-1 (HO-1) in C. carpio kidneys. Cd exposure also led to upregulation of miR-155 and miR-181a expressions. Furthermore, AKP and ACP activity in the kidneys was markedly changed after intraperitoneal injection of C. carpio with miR-155 agomir and miR-181a antagomir . All detected mRNA expressions were significantly decreased after injection of miR-155 agomir, and IL-10, NF-κB, TNF-α, and HO-1 mRNA expressions were markedly increased after injection of miR-181a antagomir. The results of this study demonstrate that Cd exposure can immunocompromise C. carpio by targeting HO-1 through miR-155 and miR-181a. This is the first study to reveal that Cd exposure induces immunotoxicity through miR-155 and miR-181a in the kidneys of C. carpio .
研究领域:
环境毒理学
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分子生物学
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鱼类免疫学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2023
·
IF 8.4
Liang Tian,Jiyuan Zhang,Yanci Zhang,Huiling Fan,Chun Liu,Yihong Wang,Jiansheng Cui
Ultrasensitive detection for low concentration and abnormal expression of microRNA-155 (miR-155) in serum (fM level) at the early stage of cancer still exist great challenges. Therefore, a dual-model photoelectrochemical biosensor was developed based on label-free trimetallic nanoprobe and Mg 2+ dependent DNAzyme driven DNA walker to generate ratiometric dual-signal output for sensitive biosensing of miR-155 from breast cancer cells (MCF-7) and cervical cancer cells (HeLa). In signal amplification strategy, PtCo@Prussian Blue nanozyme was selected as a signal nanoprobe with high catalytic activity and large specific surface area, while two dimensional MXene/AuNPs as surface modification material was applied to improve the active sites of the electrode. With the presence of the target, the DNAzyme assisted DNA walker process was initiated and abundant signal nanoprobe was introduced to the electrode surface , producing high current response and weak ultraviolet-visible spectrophotometry (UV–vis) response. Hence, the biosensor for miR-155 shows a low limit of detection of 1.2 fM from the linear range of 5 fM to 5 nM, exhibiting excellent stability and reproducibility. Moreover, the proposed biosensor demonstrates superior performance for miR-155 in complex human serum samples and tumor cell lysates, presenting great application potential for early-stage clinical diagnosis.
研究领域:
生物化学
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生物技术
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癌症研究
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纳米技术
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分析化学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2022
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IF 9.221
Yu-Yang Wei,Shao-Hua Wu,Qi-Meng Wang,Jian-Jun Sun
A novel split-type and signal-on photoelectrochemical (PEC) biosensor was proposed based on double-strand specific nuclease (DSN)-assisted recycling amplification coupled with photodeposited Ag enhanced BiVO 4 photoanode for sensitive detection of microRNA (miRNA)− 155. In the presence of target miRNA, the capture DNA (cDNA) modified with silver nanocube（AgNC）would hybridize with the miRNA, then DSN-assisted cycle amplification was triggered, leading to the release of AgNCs. The released AgNCs were acidolyzed and photodeposited on the BiVO 4 electrode. Compared with the BiVO 4 electrode, an enhanced photocurrent could be achieved at this obtained BiVO 4 /Ag electrode. And the morphology change from worm-like nanoparticles to nanosheets after Ag photodeposition was observed for the first time. This PEC signal enhancement was mainly contributed to the synergistic action of localized surface plasmon resonance (LSPR) effect of Ag, Schottky junction formed between BiVO 4 and Ag, and the formation of BiVO 4 nanosheets with larger specific surface area. The PEC signal enhancement was positively related to the miRNA-155 concentration, so this method could be used to sensitively and selectively detect miRNA-155. A limit of detection of 8 aM could be achieved. This strategy does not require layer-by-layer modification of the electrode, so it is easy to operate, and owns good stability.
研究领域:
生物化学
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分析化学
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生物传感器
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纳米技术
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分子生物学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Xiaomei Mu,Shixiu Xiao,Shulin Zhao,Jianniao Tian
In this work, a fast signal amplification system mediated by self-replicating catalytic hairpin self-assembly (SCHA) was established for microRNA-155 using near-infrared DNA-Ag Nanoclusters (DNA-AgNCs) as fluorescence signal output. Among them, two fission target-like DNA sequences are merged into two hairpin DNA H1 and H2, and the AgNCs template sequence is designed at the sticky end of H1 and H2. The target can be recycled in the system to form a double-stranded DNA structure (H1–H2), which will detach the H1/H2-AgNCs from the surface of the polypyrrole nanoparticles (PPy NPs) and cause the near-infrared fluorescence signal of DNA-AgNCs to be restored. At this point, the two-split target-like DNA sequences will be reassembled to initiator DNA. The acquired replicas can also be recycled as a brand-new activation unit to initiate the SCHA response, resulting in rapid replication of the target/triggered DNA, accompanied by the generation of higher fluorescence signals. This autocatalytic signal amplification approach has been successfully applicable to fast signal amplification, enzyme-free and label-free for microRNA-155 assay in biological samples, and the detection limit (LOD) is 240 fM (S/N = 3). At the same time, this SCHA system can realize intracellular microRNA fluorescence imaging, which presents a promising approach to developing advanced molecular diagnostic tools.
研究领域:
生物化学
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分子生物学
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医学诊断
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DOI
PubMed
高影响因子
Nanoscale Advances
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2024
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IF 4.6
Xianghang Lin,Yang Yang,Wenzhang Zhu,Xiaorong He,Yunliang Liu
MicroRNAs (miRNAs) play crucial roles in the regulation of immune cell differentiation and the immune response during allergic rhinitis (AR). Studies have shown that miRNA-155 is significantly upregulated in AR pathogenesis. Therefore, miRNA-155 can be used as a biomarker for AR diagnosis. Although fluorescent biosensors based on upconversion nanoparticles (UCNPs) have made significant advances in the detection of miRNAs, developing UCNPs with polymer coatings, efficient surface passivation, and DNA functionalization for hybrid sensing in biological media remains challenging. Herein, hairpin DNA1 (H1) is modified into a thin polysulfonic acid layer on UCNPs by sulfonamide bonds, and the fluorescence of the UCNPs is quenched by the fluorescence resonance energy transfer (FRET) process of BHQ3 carried by H1. When the target miRNA-155 is present, the hairpin structure of H1 is opened, allowing BHQ3 to move away from the UCNP surface, and the fluorescence of UCNP is restored. At the same time, hairpin DNA1 (H2) can combine with H1 to replace the miRNA-155 that is bound to H1 with the help of the opening stem ring structure of H1, and the replaced miRNA-155 can continue to react with H1 to amplify the fluorescence signal. Under the optimal experimental conditions, the linear range of miRNA-155 is 0.01–3 nM, with a detection limit of 1.14 pM. Furthermore, the constructed biosensor has been applied to determine miRNA-155 in serum samples, and the spiked recoveries range from 99.8% to 104.8%, which indicates that the developed assay has potential applications in monitoring allergic rhinitis or other miRNA related diseases.
研究领域:
生物化学
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医学诊断
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纳米技术
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2024
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IF 8.3
Xiaojian Fan,Youqiang Xing,Ze Wu,Bingjue Li,Peng Huang,Lei Liu
Sensitive and accurate miRNA detection is important in cancer diagnosis but remains challenging owing to the essential features of miRNAs, such as their small size, high homology, and low abundance. This work proposes a novel electrochemical (EC)-enhanced quantum sensor achieving quantitative detection of miRNA-155 with simultaneous EC sensing. Specifically, fluorescent nanodiamonds/MXene nanocomposites were synthesized and modified with dual-mode signal labels, enabling miRNA-155 concentration measurement via T1 relaxation time of nitrogen-vacancy (NV) centers and EC signals. Quantum sensing was enhanced via external voltage during the EC process, which modulated the negatively charged state of the NV centers, thereby improving the sensitivity and accuracy of miRNA-155 detection. EC sensing improved the accuracy and reliability of miRNA-155 detection while enhancing quantum sensing. The limit of detection (LOD) of the EC-enhanced quantum biosensor reached 10.0 aM, nearly 106 and 10 times lower than the reported LODs of a quantum sensor using bulk diamond and fluorescent sensors, respectively. The LOD of EC sensing was 2.6 aM, aligning with previous reports. The findings of the study indicated that quantum sensing combined with EC sensing can achieve ultrasensitive miRNA-155 detection with high accuracy and reliability, providing an advanced approach for early cancer diagnosis.
研究领域:
生物医学工程
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分子生物学
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纳米技术
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DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2025
·
IF 4.5
Jinshan Liu,Songyang Miao,Niewei You,Jinghan Li,Siao Li,Xun Chen,Jie Wang,Mu Yang,Kejian Ding
Early cancer diagnosis is critically enhanced by detecting microRNA (miRNA) biomarkers, such as miRNA-155, whose overexpression correlates with cancer progression. This work presents a highly sensitive photoelectrochemical (PEC) biosensor for miRNA-155 detection leveraging a modified carbon nitride (m-CN) substrate modified by ReS₂ (ReS₂/m-CN), which enhanced electron transfer efficiency and provided abundant sites. The resulting biosensor exhibits exceptional performance with a broad linear detection range from 10 −16 M to 10 −7 M, an ultralow detection limit, high selectivity, and outstanding stability. This work demonstrates the significant potential of the ReS 2 @m-CN-based PEC biosensor for sensitive, cost-effective early cancer diagnostics.
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DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2025
·
IF 5.5
Yanci Zhang,Yujin Fan,Jiyuan Zhang,Yanxi Hu,Lili Duan,Zhanhui Wang,Yihong Wang,Jiansheng Cui,Liang Tian
The imbalanced expression of microRNA is related to many diseases and tumors, which can be used as a noninvasive biomarker to assist in the diagnosis, prognosis, and monitoring of cancer. A target conversion strategy-based electrochemical biosensor was developed by DNAzyme-cleavage dual cyclic signal amplification and the strand displacement reaction (SDR) to produce high electrochemical signals for microRNA-155 (miRNA-155) analysis. Thereinto, VSe2/multi-walled carbon nanotubes (MWCNTs) with high conductivity were applied as the electrode surface material to provide more active sites and accelerate electron transportation. Then, the excellent immobilization efficiency and large specific surface area of the ZnO@CuS nanoprobes were applied as a recognition molecule carrier. Furthermore, hexaammineruthenium(III) chloride (RuHex), with superior electrochemical redox activity, acted as the electroactive indicator by intercalating into double-helix DNA through electrostatic interaction, which produced high current responses in the presence of the target. Therefore, this miRNA-155 biosensing platform exhibited a relatively low limit of detection of 0.11 fM, ranging from 0.5 fM to 5 nM, and demonstrated excellent repeatability and stability. Hence, this platform appeared to possess favorable human serum detection capacity and be suitable for early cancer diagnostics.
研究领域:
生物医学工程
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分子诊断学
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纳米技术
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DOI
高影响因子
International Journal of Nanomedicine
·
2018
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IF 4.37
Yang Li,Yanhong Duo,Jiangang Bi,Xiaowei Zeng,Lin Mei,Shiyun Bao,Lisheng He,Aijun Shan,Yue Zhang,Xiaofang Yu
Introduction MicroRNA-155 (miR-155) is an oncogenic microRNA, which is upregulated in many human cancers including colorectal cancer (CRC). Overexpression of miR-155 has been found to regulate several cancer-related pathways, and therefore, targeting miR-155 may be an effective strategy for cancer therapy. However, effective and safe delivery of anti-miR-155 to tumors remains challenging for the clinical applications of anti-miR-155-based therapeutics. Methods In this study, we explored the expression of miR-155 and the transcription factor nuclear factor kappa B (NF-κB) in CRC tissues and cell lines, and the possible relationship between miR-155 and NF-κB. We further report on anti-miR-155-loaded mesoporous silica nanoparticles (MSNs) modified with polymerized dopamine (PDA) and AS1411 aptamer (MSNs-anti-miR-155@PDA-Apt) for the targeted treatment of CRC. Results Results showed that miR-155 is overexpressed in CRC tissues and cell lines, and there is a positive feedback loop between NF-κB and miR-155. Compared to the control groups, MSNs-anti-miR-155@PDA-Apt could efficiently downregulate miR-155 expression in SW480 cells and achieve significantly high targeting efficiency and enhanced therapeutic effects in both in vivo and in vitro experiments. Furthermore, inhibition of miR-155 by MSNs-anti-miR-155@PDA-Apt can enhance the sensitivity of SW480 to 5-fluorouracil chemotherapy. Conclusion Thus, our results suggested that MSNs-anti-miR-155@PDA-Apt is a promising nanoformulation for CRC treatment.
研究领域:
肿瘤学
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纳米技术
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分子生物学
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癌症治疗
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2020
·
IF 7.1
Fenghe Duan,Chuanpan Guo,Mengyao Hu,Yingpan Song,Minghua Wang,Linghao He,Zhihong Zhang,Riccardo Pettinari,Liming Zhou
A promising nanocarrier of the complementary DNA (cDNA) toward miRNA-155 was synthesized on the basis of the nanohybrid type of Ti 3 C 2 T x MXene nanosheets decorated using iron phthalocyanine quantum dots (FePc QDs) (denoted as Ti 3 C 2 T x @FePcQDs) to construct a novel ultrasensitive impedimetric aptasensing system for microRNA-155 (miRNA-155). Owing to π–π* stacking interaction between Ti 3 C 2 T x nanosheets and FePc QDs, the homogeneous nanostructure of the Ti 3 C 2 T x @FePcQD nanohybrid that comprises mixed-valence states (Ti 2+ /Ti 3+ and Fe 2+ /Fe 3+ ), multicomponent (Ti-O and Ti-C), and various N-related groups was achieved. The constructed Ti 3 C 2 T x @FePcQDs-based aptasensor displayed an ultrahigh sensitivity for detecting miRNA-155 with a low detection limit of 4.3 aM (S/N = 3) within the miRNA-155 concentration ranging from 0.01 fM to 10 pM. Compared with the individual component-based aptasensors and other reported miRNA-155 aptasensors, the proposed impedimetric aptasensing system exhibited substantial merits of a feasible preparation process, non-use of labels or electrochemical indicators, fast response time, and comprehensive sensing performances for detecting miRNA-155. This strategy for determining miRNAs can extensively be applied as the platform for anchoring other kinds of aptamers in detecting diverse targets, thus indicating its great potential application for the early diagnosis of cancer biomarkers.
研究领域:
纳米技术
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生物传感器
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分析化学
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分子生物学
·
癌症研究
✓ 已验证
DOI
高影响因子
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