Analytical Methods
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2015
·Vol. 7
·期 16
·6712-6717
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IF 1.821
Weiwen Hu,Yong Ning,Lianzhi Li,Jinming Kong,Xueji Zhang
In this work, a highly effective biosensor supported on morpholino-functionalized magnetic microspheres was investigated for the sequence-specific detection of DNA. Briefly, morpholino was first modified on the surface of magnetic microspheres through amido bonds, and then hybridized with target DNA in the ensuing step. Biotin-labeled signal probe DNA was designed to hybridize with the DNA nearby the 5′ end of sequence, and the biotin was used to bind with streptavidin-alkaline phosphatase (SA-ALP). L-Ascorbic acid-2-phosphate (AAP) was hydrolyzed by ALP to generate L-ascorbic acid, which could reduce resazurin to resorufin, resulting in a turn-on fluorescence signal. UV-vis absorbance spectroscopy was applied to prove the conjugation of ALP with signal probe DNA. Under optimal conditions, this biosensor displayed a good linear relationship between the fluorescence intensity and logarithm of single-stranded DNA concentrations in the range of 0.1 pM to 0.1 nM with a low detection limit of 9.32 fM. Moreover, fully complementary versus single-base mismatched, three-base mismatched and non-complementary DNA could be effectively distinguished. In addition, this novel approach rendered satisfactory analytical results for the determination of DNA in serum, thus exhibiting practical significance. These results demonstrated that this assay method displays excellent sensitivity and specificity for DNA detection and has great potential for practical applications.
研究领域:
生物化学
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分子生物学
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高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 6
·2406-2412
·
IF 1.821
Weiwen Hu,Qiong Hu,Lianzhi Li,Jinming Kong,Xueji Zhang
In this work, an efficient method for the sequence-specific detection of DNA based on a morpholino-functionalized silicon chip platform has been proposed. Briefly, morpholino was first immobilized on the surface of a silicon chip using 3-aminopropyltriethoxysilane (APTES) as the silane coupling agent and 1,4-phenylenediisothiocyanate (PDITC) as the cross-linker and then hybridized with DNA in the ensuing step. The fluorescence label was introduced by strongly binding Rhodamine B, which contains a terminal carboxylic group, with DNA by means of phosphate–zirconium–carboxylate coordination reaction. X-ray photoelectron spectroscopy (XPS) was used to characterize the silicon surface. Under optimal conditions, the morpholino-functionalized silicon chip presented a great linear relationship between the fluorescence intensity and the logarithm of target DNA concentrations in the range from 1 pM to 1 nM with a detection limit of 4.52 pM. Furthermore, fully complementary versus single-base mismatched, three-base mismatched and non-complementary DNA could be effectively identified. The chip showed excellent stability because it could be reused for another hybridization experiment after denaturing the morpholino–complementary DNA duplex. In addition, the chip rendered satisfactory analytical performance for the detection of DNA in serum samples, thus exhibiting practical significance. Morpholino-functionalized silicon chips display good sensitivity and selectivity for the detection of DNA and promising applications in single-nucleotide polymorphisms (SNPs).
研究领域:
生物化学
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分子生物学
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分析化学
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高影响因子
BIOSENSORS & BIOELECTRONICS
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2021
·
IF 10.618
Jiahao Li,Ding Wu,Yi Yu,Tingxian Li,Kun Li,Meng-Meng Xiao,Yirong Li,Zhi-Yong Zhang,Guo-Jun Zhang
SARS-CoV-2 RNA is identified as a pivotal player to bolster energizing zones of COVID-19 detection. Herein, we develop a rapid and unamplified nanosensing platform for detection of SARS-CoV-2 RNA in human throat swab specimens. A gold nanoparticle (AuNP)-decorated graphene field-effect transistor (G-FET) sensor was fabricated, after which complementary phosphorodiamidate morpholino oligos (PMO) probe was immobilized on the AuNP surface. This sensor allowed for highly sensitive testing of SARS-CoV-2 RdRp as PMO does not have charges, leading to low background signal. Not only did the method present a low limit of detection in PBS (0.37 fM), throat swab (2.29 fM), and serum (3.99 fM), but also it achieved a rapid response to COVID-19 patients’ samples within 2 min. The developed nanosensor was capable of analyzing RNA extracts from 30 real clinical samples. The results show that the sensor could differentiate the healthy people from infected people, which are in high agreement with RT-PCR results (Kappa index = 0.92). Furthermore, a well-defined distinction between SARS-CoV-2 RdRp and SARS-CoV RdRp was also made. Therefore, we believe that this work provides a satisfactory, attractive option for COVID-19 diagnosis.
研究领域:
生物医学工程
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纳米技术
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病毒学
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分子诊断
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DOI
PubMed
高影响因子
DESALINATION
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2019
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IF 6.035
Shao-Lu Li,Pengfei Wu,Juntao Wang,Jing Wang,Yunxia Hu
It is well known that the water flux and antifouling performance of polyamide (PA) membrane are strongly associated with their surface characters, especially for surface hydrophilicity , roughness, and charge. In this study, a zwitterionic surface TFC reverse osmosis (RO) membrane was fabricated by small molecule 3-bromopropionic (3-BPA) modification on the pristine membrane that fabricated through interfacial polymerization (IP) technique using a new aqueous monomer 4-morpholinobenzene-1,3-diamine (MpMPD) and trimesoyl chloride (TMC). The as-fabricated RO membranes were well analyzed by various techniques, that the zwitterionic MpMPD-PA membrane surface exhibited greatly enhanced hydrophilicity with water contact angle of 28.1° and surface roughness as low as 26.0 nm. The water permeability of membrane MpMPD-PA reached 1.65 L m −2 h −1 bar −1 , which exhibited ~24% increase compared to our lab-made MPD membrane, meanwhile kept a high NaCl rejection of 97.8%. In addition, the dynamic fouling experiments illustrated that the zwitterionic surface RO membrane possess excellent fouling resistance to protein BSA, polysaccharides SA , and surfactant SDS. Therefore, this work provide a simple and convenient way to fabricate novel zwitterionic surface RO membrane with high performance from the molecular level design.
研究领域:
材料科学
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环境工程
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高影响因子
NEW JOURNAL OF CHEMISTRY
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2013
·Vol. 37
·期 5
·1437-1444
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IF 2.966
Shang Gao,Hongling Guo,Xiaojun Peng,Xing Zhao,Qian Duan,Qingcheng Liang,Dayong Jiang
A tris(morpholino)phosphine (TMP) ligand was introduced into the diiron dithiolate complexes in order to improve the hydro- and protophilicity of the iron-only hydrogenase active site models. Mono- and di-TMP substituted diiron complexes, (μ-pdt)[Fe(CO)3][Fe(CO)2(TMP)] (2, pdt = 1,3-propanedithiolato) and (μ-pdt) [Fe(CO)2(TMP)]2 (3), were synthesized and spectroscopically characterized. The coordination configuration of 3 was determined by single X-ray analysis. Temperature-dependent 1H and 31P NMR spectroscopy studies provided insight into the interconversion of the irondithiacyclohexane ring and the rotation of the [Fe(CO)2PR3] moieties for 2 and 3 in solution. The electrochemical properties of 2 and 3 were investigated in pure CH3CN and CH3CN–H2O mixtures in the absence and presence of acetic acid. Hydrogen production and the dependence of current on acid concentration indicated that complexes 2 and 3 exhibited electrocatalytic activities for proton reduction in both pure and H2O-containing CH3CN solutions. The current sensitivities, i.e., electrocatalytic activities, were demonstrated to be greater in CH3CN–H2O mixtures than in pure CH3CN. The most effective electrocatalytic activities of 2 and 3 were observed with 10% added water.
研究领域:
无机化学
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催化化学
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DOI
高影响因子
MACROMOLECULAR CHEMISTRY AND PHYSICS
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2023
·Vol. 225
·期 5
·2300361
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IF 2.5
Dan Liu,Hui Liang,Jiang Lu
A novel monomer, N -(2-((3-morpholine-3-isopropyl)thio)ethyl)methacrylamide (MTMA), is synthesized and polymerized by reversible addition-fragmentation chain transfer (RAFT) polymerization to give a thermoresponsive polymer poly[ N -(2-((3-morpholino-3-oxopropyl)thio)ethyl)methacrylamide] (PMTMA). PMTMA exhibits reversible thermoresponsive phase transitions of the lower critical solution temperature (LCST) type in water and the upper critical solution temperature (UCST) type in ethanol and methanol. The degree of polymerization and concentration of PMTMA, and solvent type have a significant influence on the transition temperature. PMTMA shows complicated thermoresponse in water–alcohol mixtures. In water–ethanol mixtures, the phase diagram of PMTMA can be divided into three regions according to ethanol volume percentages, φ : 0 ≤ φ < 15% for an LCST region; 15% ≤ φ < 91.5% for a complete dissolution region; 91.5% ≤ φ ≤ 100% for a UCST region. At high water content, the LCST increases with increasing ethanol content; and at high ethanol content, the UCST decreases with increasing water content. In water–methanol mixtures, PMTMA shows more complex thermoresponse. In the range of 0–50 °C, the phase diagram can be divided into five regions, depending on the methanol volume percentages φ : 0 ≤ φ < 15% for LCST region; 15% ≤ φ < 25% for immiscible region; 25% ≤ φ < 85% for UCST region; 85% ≤ φ < 95% for fully dissolved region; 95% ≤ φ ≤ 100% for another UCST region.
研究领域:
高分子化学
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材料科学
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物理化学
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DOI
高影响因子
危险品化学品经营许可证（带存储）