CHEMISTRY & BIODIVERSITY
·
2020
·Vol. 17
·期 7
·e2000241
·
IF 2.039
Nan Wang,Yuan Dong,Yu Yang,Rui Xu,Chang-Wei Li,Cheng-Bin Cui
Penicimutanin C, a new alkaloidal compound, was isolated from the neomycin-resistant mutant strain 3-f-31 of the marine-derived fungus, Penicillium purpurogenum G59, together with four known compounds. The structure of penicimutanin C, including the absolute configuration, was determined by spectroscopic and chemical methods. The absolute configuration of penicimutanin A was also re-confirmed by Marfey's and chiral HPLC analyses of the hydrolyzed products. Penicimutanins C and A inhibited the proliferation of five human cancer cell lines to some extent. Penicimutanin C is the third dimer of diketopiperazine and penicimutanolone, which are only produced by mutants of P. purpurogenum G59 isolated to date, and it showed cytotoxic activity against human cancer cell lines. The neomycin-resistant screening strategy has been previously successfully used to discover new compounds by activating silent metabolites in fungi, and the present results provide an additional example of the effectiveness of this method. Graphical
研究领域:
药理学
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微生物学
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肿瘤学
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DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
·
2025
·
IF 3.3
Jie Mingsha,Zhu Amei,Zhu Bing,Guo Ruipeng,Lan Shengkai,He Jiaxin,Lang Xingang,Bai Yanhong
The overuse of antibiotics leads to significant health risks and environmental challenges. Therefore, selective and sensitive detection of antibiotic residues is a critical issue. Here, a double-emission ratiometric fluorescence probe (Lu-Eu-DPA) was constructed for the selective detection of gentamicin sulfate (GEN), neomycin (NEO), and erythromycin (EM). The Lu-Eu-DPA coordination polymer nanoparticles composed of luminol, the coordinated nuclear metal Eu 3+ , and 2,6-pyridinedicarboxylic acid (DPA). The coordination reaction between luminol molecules and Eu 3+ ions produce the aggregation-induced emission (AIE) effect, while DPA acts as a ligand for the antenna, absorbing energy from the excitation light and then transferring the energy to Eu 3+ . Based on the AIE effect and antenna effect (AE), the fluorescence intensity of Lu-Eu-DPA at 426 nm and 617 nm was correlated with the concentration of GEN, NEO, and EM, and the detection limits of GEN, NEO, and EM were 20, 25, and 48 nmol/L, respectively. The detection capability of the proposed method in pork samples was also studied. In addition, semi-quantitative determination of GEN, NEO, and EM was conveniently and efficiently performed using a smartphone-based visualization method. These results suggested that this probe with good selectivity and specificity was a promising method for swiftly detecting multiple antibiotic residues.
研究领域:
分析化学
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食品安全
·
生物医学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Yuefei Li,Jiaqi Hou,Dong Wang,Junhao Ma,Weikai Sun,Mingxiao Li,Zhihao Sun,Yulin Liu,Guocheng Liu,Qinghua Yan,Qianwen Wang,Beidou Xi,Huiling Liu,Yanjun Xin
The neomycin fermentation residues (NFR) are a solid waste during neomycin production, possessing high recycling value due to their rich organic content. However, the environmentally friendly use of NFR was hindered by its rigid matrix structure and residual neomycin. In this study, the effect of temperature on the structure and bioresource properties of NFR were investigated. Meanwhile, the effect of temperature on the degradation of residual neomycin, the toxicity of neomycin degradation products, and the safety of NFR were assessed. The results indicated that the content of soluble organic matter was increased by appropriate temperature treatment through the disruption of the rigid matrix structure of the NFR. However, excessively high temperatures led to the mineralization of organic matter. Simultaneously, appropriate temperature facilitated the transformation of organic substances to humic-like acids. The neomycin in NFR was effectively degraded with the increasing temperature, and the degradation efficiency exceeded 99 % under 220 °C for 120 min. In addition, the applicability of using appropriate temperatures to degrade antibiotics in similar other AFR has been demonstrated. Neomycin degradation occurred primarily through the cleavage of the glycosidic bond and ring ether, the detachment of the amino group, and the addition of hydroxyl groups. Meanwhile, the developmental toxicity and acute toxicity of neomycin were effectively mitigated by the appropriate temperature, and a significant reduction in ecotoxicity was observed. As a result, optimum temperature treatment proved to be an efficient method for the secure recycling of NFR.
研究领域:
环境科学
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微生物学
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药理学
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DOI
高影响因子
CHROMATOGRAPHIA
·
2021
·Vol. 84
·期 9
·861-868
·
IF 2.044
Huang Yejing,Han Xiufen,Yu Xiao,Wang Shumei,Zhai Haiyun
A capillary electrophoresis-indirect laser-induced fluorescence detection method was established for neomycin detection in fish. With rhodamine 6G as the background fluorescent substance, neomycin with weak ultraviolet absorption can be detected effectively. The types and concentrations of background buffer, pH, and separation voltage which affected the separation and analysis were all studied. With the optimal conditions, the limit of detection of neomycin was 5.0 ng/g, the recovery of fish samples is between 95.2 and 99.7%, and the relative standard deviation is between 2.7 and 3.6%.
研究领域:
分析化学
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食品科学
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产品关联
DOI
高影响因子
Frontiers in Molecular Neuroscience
·
2022
·
IF 6.261
Yunhao Wu,Wei Meng,Ming Guan,Xiaolong Zhao,Chen Zhang,Qiaojun Fang,Yuhua Zhang,Zihui Sun,Mingjing Cai,Dongdong Huang,Xuechun Yang,Yafeng Yu,Yong Cui,Shuangba He,Renjie Chai
Irreversible injury to inner ear hair cells induced by aminoglycoside antibiotics contributes to the formation of sensorineural hearing loss. Pitavastatin (PTV), a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor, has been reported to exert neuroprotective effects. However, its role in aminoglycoside-induced hearing loss remains unknown. The objectives of this study were to investigate the beneficial effects, as well as the mechanism of action of PTV against neomycin-induced ototoxicity. We found that PTV remarkably reduced hair cell loss in mouse cochlear explants and promoted auditory HEI-OC1 cells survival after neomycin stimulation. We also observed that the auditory brainstem response threshold that was increased by neomycin was significantly reduced by pretreatment with PTV in mice. Furthermore, neomycin-induced endoplasmic reticulum stress in hair cells was attenuated by PTV treatment through inhibition of PERK/eIF2α/ATF4 signaling. Additionally, we found that PTV suppressed the RhoA/ROCK/JNK signal pathway, which was activated by neomycin stimulation in HEI-OC1 cells. Collectively, our results showed that PTV might serve as a promising therapeutic agent against aminoglycoside-induced ototoxicity.
研究领域:
药理学
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耳鼻喉科学
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神经科学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Xinyu Cheng,Yifan Lei,Shuyang Zhang,Liping Chen,Yongzhong Wang
Hyperuricemia (HUA), a metabolic disorder resulting from disrupted uric acid (UA) homeostasis, is the principal etiological factor for gout. This study investigated the therapeutic potential of gut microbiota modulation in HUA using antibiotic-treated mouse models with butyrate intervention. Selective antibiotic screening identified the Dubosiella-type strain Dubosiella newyorkensis (Dn) as an effective intervention for HUA. Dn treatment significantly reduced UA production, improved renal function markers, and decreased oxidative stress. Dn enriches Bacteroides and Romboutsia while suppressing the pathobiont Thomasclavelia, thereby enhancing the production of acetate, propionate, and butyrate. Molecular analyses demonstrated that Dn downregulated the expression of URAT1 while upregulating ABCG2, thereby promoting UA excretion. Histopathological evaluation confirmed that Dn restored the glomerular atrophy, tubular dilation, and collagen deposition induced by HUA. These findings suggest that Dn is a promising multitarget therapeutic agent for HUA and provides a scientific foundation for the development of novel microbiome-based strategies against HUA.
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2019
·
IF 3.973
Yahui Zhang,Yi Yuan,Wei Chen,Jie Fan,Hong Lv,Qingsheng Wu
The abuse of antibiotics has led to widespread antimicrobial resistance (AMR) and environmental pollution. In order to solve these problems, a lot of studies have been carried out mainly focusing on the modification and recombination of organic reagents, but bacteria are still easy to adapt to it, so they cannot be thoroughly solved. Here, we present an integrated pollution-free synergistic antibacterial nanotechnology using inorganic nano-Cu 2 O, which could not only enhance the efficacy of aminoglycoside antibiotics, but also eliminate their environmental pollution by photocatalytic degradation. It was found that Cu 2 O showed significantly synergistic antibacterial effect (1+1>2) when combined with aminoglycoside antibiotics against Escherichia coli . The inhibition zone area increased by 59.0% when Cu 2 O combined with neomycin. This reduces dosage and the risk of AMR, and does not pollute the environment after degradation. Next, to explore the synergistic mechanisms, we have studied the interaction of antibiotics with nanoparticles, as well as the interaction of antibacterial agents with bacteria. At last, we believe that the destruction of cell walls by Cu 2 O facilitates the entry of antibiotics into cells is the reason for their synergy.
研究领域:
微生物学
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环境科学
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纳米技术
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药理学
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产品关联
DOI
PubMed
高影响因子
ACS Sensors
·
2022
·
IF 9.618
Li-Ping Zong,Xiaozhong Chen,Dunru Zhu,Xi-Jie Li,Feng Li,Serge Cosnier,Xue-Ji Zhang,Robert S. Marks,Dan Shan
A novel electrochemiluminescence (ECL) amplification strategy was established aiming to overcome the inherent shortcomings of the current oxygen (O2) coreactant ECL systems. Macrocyclic Schiff base Fe complexes were rationally designed as a novel integrated ECL emitter by iminium linkage between N-(4-aminobutyl)-N-ethylisoluminol (ABEI) and 1,10-phenanthroline-2,9-dicarbaldehyde (PDL) and postmetalation of the macrocyclic Schiff base. Covalently combining luminophore ABEI with a catalytic center endowed the novel ECL emitter with both remarkable redox electrocatalytic properties and significantly enhanced ECL efficiency. The high content of ferrous iron and the dominantly active low-spin Fe state greatly contributed to the inherent catalytic activity for O2 activation. The rational modification of luminophore optimized the spatial distribution and simultaneously shortened the species transport distance of coreactant radicals generated in situ from dissolved O2, resulting in significantly self-enhanced ECL efficiency. Neomycin, which posed a growing threat to aquatic biodiversity and environmental safety, as the model antibiotic was successfully detected with a detection limit of 0.21 pM (S/N = 3), clarifying a promising application prospect of this new luminophore-embedded ECL amplification strategy in biological analysis and environmental monitoring.
研究领域:
分析化学
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环境科学
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生物分析
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产品关联
DOI
PubMed
高影响因子
ACTA OTO-LARYNGOLOGICA
·
2024
·
IF 1.2
Nishan Chen,Tongxiang Diao,Jilei Zhang,Lisheng Yu
Backgrounds Due to the therapeutic needs of inner ear diseases, a simple method of inner ear drug delivery has been under exploration. Postauricular administration, which involves injecting the drug through the middle of the retroauricular groove, is a potential novel approach, but its mechanisms remain unclear.Objectives To investigate threshold shifts in the auditory brainstem response (ABR), as well as the morphology and quantity of hair cells on the bilateral inner ear of guinea pigs after postauricular neomycin administration.Material and Methods Thirty healthy guinea pigs were divided randomly into two groups and administered either neomycin 200 mg/kg or saline 1 ml via daily postauricular injection for 10 days. Auditory threshold-shifts were examined by tests of the ABR. The quantity and morphology of hair cells in the organ of Corti were assessed by confocal laser scanning microscopy.Results The ABR results showed that at a frequency of 16 kHz, the threshold shift was 21.00 ± 14.49 dB in the neomycin injection side, 10.00 ± 16.67 dB in the contralateral side, and 0.50 ± 3.69 dB in the saline group. At a frequency of 24 kHz, the threshold shift was 37.00 ± 16.36 dB in the neomycin injection side, 14.50 ± 9.26 dB in the contralateral side, and 1.00 ± 2.11 dB in the saline group. Histopathological analysis revealed that the outer hair cells (OHCs) in the neomycin injection side suffered significant morphological and numerical loss, while the OHC loss in the contralateral ear was less severe than in the injection side but greater than in the saline group.Conclusions and Significance This study confirms that postauricular administration is a novel method of local drug delivery to the inner ear, which has practical value in future experimental modeling and the treatment of inner ear diseases.
研究领域:
耳科学
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药理学
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产品关联
DOI
PubMed
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2022
·
IF 3.268
Kemeng Qin,Mingyue Ding,Chensi Zhang,Xijie Zhang,Yexuan Mao,Meng Dang,Zizhe Li,Youyi Wang,Shaohui Zhang,Yuhang Sun,Zhibin Zhang,Gaiming Zhao,Yu Li,Qian Li,Xiya Zhang
To improve the detection sensitivity of neomycin (NEO) in milk, we produced a sensitive monoclonal antibody (mAb) against NEO and developed a lateral flow immunoassay based on amorphous carbon nanoparticles (ACNPs-LFA). First, we conjugated NEO to carrier protein to prepare mAbs. We obtained six mAbs: mAb 1C6, mAb 1D3, mAb 2D3, mAb 4D5, mAb 5D1, and mAb 5H1. We characterised the mAbs by indirect competitive enzyme-linked immunosorbent assay and selected the most sensitive mAb based on the half-maximal inhibitory concentration (IC50). We selected mAb 4D5 (IC50 = 0.15 ng/mL) for the development of LFA. MAb 4D5 was labelled with ACNPs (ACNPs-mAb 4D5) by electrostatic absorption. Under optimised conditions, 5.4 μg mAb 4D5 coupling with 1 mL ACNPs, NEO-OVA at concentration of 80 μg/mL, 3 μL ACNPs-mAb 4D5 were used to develop LFA. The cut-off value was 8 ng/mL. Therefore, our developed ACNPs-LFA is suitable for on-site detection of NEO residues.
研究领域:
食品安全
·
免疫学
·
分析化学
✓ 已验证
DOI
高影响因子
INORGANICA CHIMICA ACTA
·
2025
·
IF 3.2
Kai-Jie Hu,Wen-Hui Gu,Yan Zhou,Jing Chen
As a new class of porous crystalline materials, hydrogen-organic frameworks (HOFs) have attracted lots of attention in a variety of applications. Despite their potential, hydrogen-bonded organic frameworks (HOFs)-based fluorescent sensing materials have received relatively limited research attention to date. In this work, we successfully constructed a fluorescence sensor based on HOFs constructed by 1,3,6,8-tetrakis[ p -benzoic acid]pyrene, which can display high sensitivity and fluorescence quenching selectivity for neomycin sulphate (NS).
研究领域:
材料科学
·
分析化学
·
生物医学
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产品关联
DOI
高影响因子
CHROMATOGRAPHIA
·
2013
·Vol. 76
·期 9
·459-465
·
IF 1.437
Peng Xi-Tian,Feng Yu-Qi,Hu Xi-Zhou,Hu Ding-Jin
In this paper, a novel hydrophilic neomycin-bonded silica (Silica-NC) stationary phase for hydrophilic-interaction chromatography (HILIC) was prepared with cyanuric chloride as coupling agent. The resulting Silica-NC stationary phase was characterized by Fourier transform infrared spectroscopy and elemental analysis to prove the successful immobilization of neomycin on the surface of silica gel. A set of analytes with different properties were selected to investigate the chromatographic characteristics of the Silica-NC stationary phase under HILIC mode. The effects of mobile phase variables such as organic modifier content, ionic strength and pH values on the chromatographic behaviors of testing probes were investigated in detail. The results demonstrated that the Silica-NC phase behaved as a chromatographic packing with hydrophilic-interaction and ionic-interaction. Finally, the effective separations of nucleosides and bases, organic acids, cytokinins and sulfanilamides were achieved on the Silica-NC stationary phase under HILIC mode, indicating the excellent application potential of our developed hydrophilic Silica-NC stationary phase.
研究领域:
分析化学
·
色谱技术
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产品关联
DOI
高影响因子
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