JOURNAL OF THE WORLD AQUACULTURE SOCIETY
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2022
·Vol. 53
·期 6
·1084-1100
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IF 3.402
Wei-Wei Mao,Zi-Chen Zhao,Gui-Hua Li,Wei-Liang Guo,Ze-Hao Ding,Shi-Feng Wang,Yun Sun,Zhen-Jie Cao,Jian-Long Li,Yong-Can Zhou
Cryptocaryon irritans , one of the most devastating holotrichous ciliated protozoans, causes massive economic losses in the pompano, Trachinotus ovatus , mariculture industries. Considering the many advantages of phytochemicals, such as low cost, low residuals, and low pollution, they can be a good alternative for the prevention and control of C. irritans infestations. In this study, 14 phytochemicals were evaluated for their antiparasitic activity against the theront of C. irritans , and nine showed significant antiparasitic activity. Nerolidol was the most promising compound, causing 100% mortality of theronts with 30-min exposure at a concentration of 10.0 μl/L. The half-maximal inhibitory concentrations ( IC 50 s) of nerolidol on C. irritans theronts, protomonts, and tomonts were 2.1, 13.3, and 7.1 μl/L, respectively. Abnormal morphologies of the theronts, trophonts, and tomonts were observed after exposure to 10.0, 30.0, and 10.0 μl/L nerolidol solution for 15 min, 30 min, and 480 min, respectively. A diet supplemented with 2.3 ml/kg of nerolidol reduced the number of trophonts on the gills of pompano by 95.7% decrease compared with the control groups and the theront chemotaxis to pompano mucus and sera, while increased the survival rate of infected pompanos by 24.44%, and had no significant effect on pompano hindgut and serum myeloperoxidase. These results suggest that nerolidol is a new potential drug for controlling fish cryptocaryonosis.
研究领域:
水产养殖学
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寄生虫学
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药理学
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DOI
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
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2025
·
IF 6.8
Xinrui Li,Guangtong Gao,Shiping Tian,Boqiang Li,Guozheng Qin,Yuying Wang
Plant-derived essential oils (EOs) offer a promising alternative as potential natural antimicrobial agents. However, the unclear mechanism of their components limits their precise application. Nerolidol, a sesquiterpene alcohol abundant in EOs of various plants, has known antifungal activities, but its mechanism against Botrytis cinerea is unclear. In this study, the inhibitory activity of nerolidol on B. cinerea and the underlying mechanism were investigated. Nerolidol inhibited growth and spore germination of B. cinerea , and effectively controlled grey mold in strawberry and tomato fruit. Transcriptomic analysis revealed that nerolidol reprogrammed the metabolic pathways of B. cinerea , leading to changes in many important metabolism-related genes. Specifically, nerolidol caused disruption of peroxisome structure, downregulation of hydrogen peroxide (H 2 O 2 ) and lipid metabolism, and accumulation of reactive oxygen species (ROS). The ROS scavenger N-acetylcysteine can partially reverse the growth inhibition of B. cinerea and alleviate peroxisome damage. Our results suggest that peroxisome disruption and H 2 O 2 accumulation contribute to the antifungal activity of nerolidol against B. cinerea .
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DOI
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Yu Zhang,Fengrui Li,Honglei Yan,Yan Zhang,Shimiao Feng,Lei Deng,Lixia Zhao,Shuang Gao,Ying Fu,Fei Ye
The development of bio-based active food packaging is urgently needed to address food safety concerns. In this study, nerolidol/hydroxypropyl- β -cyclodextrin inclusion complex nanofibers (Nerolidol/HP β CD-IC-NF) were prepared by electrospinning to improve the thermal stability and water solubility of natural antimicrobial nerolidol. Structural characterization (FTIR, XRD, 1 H NMR) confirmed successful nerolidol encapsulation within HP β CD cavities. The resulting bead-free nanofibers exhibited high surface area and porosity. Nerolidol/HP β CD-IC-NF demonstrated a 3.3-fold increase in free radical scavenging capacity compared to free nerolidol, along with potent antimicrobial activity (88.1 % and 76.0 % inhibition against E. coli and S. aureus , respectively). In strawberry preservation trials, nanofiber membranes reduced B. cinerea lesion diameters by 5.56-fold after 5 days compared to untreated controls, significantly extending shelf life. These findings highlight Nerolidol/HP β CD-IC-NF as a promising solution for preventing microbial contamination and improving food preservation efficacy in active packaging applications.
研究领域:
食品科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
CELL BIOCHEMISTRY AND FUNCTION
·
2023
·Vol. 42
·期 1
·e3899
·
IF 3.6
Tingting Wang,Guihua Song,Mengmeng Sun,Yan Zhang,Bingxue Zhang,Minghao Peng,Mengyin Li
Asthma is a common respiratory disease associated with airway inflammation. Nerolidol is an acyclic sesquiterpenoid with anti-inflammatory properties. BALB/C mice were sensitized with ovalbumin (OVA) to induce asthma symptoms and given different doses of Nerolidol. We found that Nerolidol reduced OVA-induced inflammatory cell infiltration, the number of goblet cells and collagen deposition in lung tissue. Nerolidol reduced the OVA-specific IgE levels in serum and alveolar lavage fluid in an asthma model. Immunohistochemical staining of α-SMA (the marker of airway smooth muscle) showed that Nerolidol caused bronchial basement membrane thinning in asthmatic mice. The hyperplasia of airway smooth muscle cells (ASMCs) is an important feature of airway remodeling in asthma. ASMCs were treated with 10 ng/mL TGF-β to simulate the pathological environment of asthma in vitro and then treated with different doses of Nerolidol. Nerolidol inhibited the activity of TGF-β/Smad signaling pathway both in the lung tissue of OVA-induced mouse and TGF-β-stimulated ASMCs. 16s rRNA sequencing was performed on feces of normal mice, the changes of intestinal flora in OVA-induced asthmatic mice and Nerolidol-treated asthmatic mice were studied. The results showed that Nerolidol reversed the reduced gut microbial alpha diversity in asthmatic mice. Nerolidol changed the relative abundance of gut bacteria at different taxonomic levels. At the phylum level, the dominant bacteria were Bacteroidota, Firmicutes, and Proteobacteria. At the genus level, the dominant bacteria were Lactobacillus, Muribaculaceae, Bacteroides , and Lachnospiraceae . We conclude that Nerolidol attenuates OVA-induced airway inflammation and alters gut microbes in mice with asthma via TGF-β/Smad signaling. Significance statement This research has demonstrated a mitigating effect of Nerolidol on the progress of asthma. TGF-β/Smad signaling pathway is the critical target of Nerolidol. Intestinal flora disorders induced by asthma are also regulated by Nerolidol.
研究领域:
药理学
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免疫学
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微生物学
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呼吸系统疾病
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DOI
PubMed
高影响因子
BIOFACTORS
·
2024
·
IF 5
Yining Lei,Manqin Li,Xinran Liu,Lu Zhang,Ruyi Zhang,Fei Cai
Despite the observation of diabetes-induced brain tissue damage and impaired learning and memory, the underlying mechanism of damage remains elusive, and effective, targeted therapeutics are lacking. Notably, the NLRP3 inflammasome is highly expressed in the hippocampus of diabetic individuals. Nerolidol, a naturally occurring compound with anti-inflammatory and antioxidant properties, has been identified as a potential therapeutic option for metabolic disorders. However, the ameliorative capacity of nerolidol on diabetic hippocampal injury and its underlying mechanism remain unclear. Network pharmacology and molecular docking was used to predict the signaling pathways and therapeutic targets of nerolidol for the treatment of diabetes. Then established a diabetic rat model using streptozotocin (STZ) combined with a high-fat diet and nerolidol was administered. Morris water maze to assess spatial learning memory capacity. Hematoxylin and eosin and Nissl staining was used to detect neuronal damage in the diabetic hippocampus. Transmission electron microscopy was used to detect the extent of damage to mitochondria, endoplasmic reticulum (ER) and synapses. Immunofluorescence was used to detect GFAP, IBA1, and NLRP3 expression in the hippocampus. Western blot was used to detect apoptosis (Bcl-2, BAX, and Cleaved-Caspase-3); synapses (postsynaptic densifying protein 95, SYN1, and Synaptophysin); mitochondria (DRP1, OPA1, MFN1, and MFN2); ER (GRP78, ATF6, CHOP, and caspase-12); NLRP3 inflammasome (NLRP3, ASC, and caspase-1); inflammatory cytokines (IL-18, IL-1β, and TNF-α); AKT (P-AKT); and mitogen-activated protein kinase (MAPK) pathway (P-ERK, P-p38, and P-JNK) related protein expression. Network pharmacology showed that nerolidol's possible mechanisms for treating diabetes are the MAPK/AKT pathway and anti-inflammatory effects. Animal experiments demonstrated that nerolidol could improve blood glucose, blood lipids, and hippocampal neuronal damage in diabetic rats. Furthermore, nerolidol could improve synaptic, mitochondrial, and ER damage in the hippocampal ultrastructure of diabetic rats by potentially affecting synaptic, mitochondrial, and ER-related proteins. Further studies revealed that nerolidol decreased neuroinflammation, NLRP3 and inflammatory factor expression in hippocampal tissue while also decreasing MAPK pathway expression and enhancing AKT pathway expression. However, nerolidol improves hippocampal damage in diabetic rats cannot be shown to improve cognitive function. In conclusion, our study reveals for the first time that nerolidol can ameliorate hippocampal damage, neuroinflammation, synaptic, ER, and mitochondrial damage in diabetic rats. Furthermore, we suggest that nerolidol may inhibit NLRP3 inflammasome and affected the expression of MAPK and AKT. These findings provide a new experimental basis for the use of nerolidol to ameliorate diabetes-induced brain tissue damage and the associated disease.
研究领域:
药理学
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神经科学
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糖尿病研究
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分子生物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2025
·Vol. 26
·期 5
·2251
·
IF 4.9
Canhong Wang,Yulan Wu,Bao Gong,Xiangsheng Zhao,Hui Meng,Junyu Mou,Xiaoling Cheng,Yinfeng Tan,Jianhe Wei
Dalbergia odoriferais widely used to treat cardiovascular diseases. Our research group found thatDalbergia odoriferavolatile oil has a good anti-myocardial ischemic effect, and its main pharmacodynamic components are trans-nerolol and its oxides. However, the exact mechanisms underlying this effect have not yet been elucidated. This study aimed to explore the potential myocardial protective effects of trans-nerolol and its underlying molecular mechanisms. Molecular docking was used to predict and visualize the possible mechanism of the anti-apoptotic myocardial protection by trans-nerolol. The myocardial protective effect of trans-nerolol was evaluated by observing pathological injury, myocardial enzyme levels, oxidation, antioxidant levels, and the expression of related proteins. Molecular docking results showed that trans-nerolol binds closely to cytochrome C (Cytc) and apoptosis-related proteins, suggesting that it may play a role in interacting with these target proteins. The results showed that pre-treatment with dose-dependent trans-nerolol significantly mitigated the myocardial histological damage; decreased lactate dehydrogenase (LDH), creatinine kinase (CK), alanine transaminase (ALT), and aspartate transaminase (AST) levels; reduced nitric oxide (NO) production, hydrogen peroxide (H2O2), and lipid peroxide (LPO); and increased the total antioxidant content (T-AOC), glutathione (GSH), catalase (CAT), and superoxide dismutase (SOD) activities compared with the model group. In addition, dose-dependent trans-nerolol significantly increased the Na+-K+-ATPase and Ca2+-Mg2+-ATPase levels. Moreover, trans-nerolol markedly reduced the endogenous and external apoptotic pathways; downregulated the protein expression of Cytc, apoptotic protease activating factor-1 (Apaf1), Fibroblast-associated (Fas), Cysteine-aspartate protease 3 (Caspase3), Cysteine-aspartate protease 8 (Caspase8), and Cysteine-aspartate protease 9 (Caspase9); and upregulated the expression of Heat shock protein 70 (Hsp70) and B-cell lymphoma-2 (Bcl-2). These data indicate that trans-nerolol exerts protective effects against myocardial ischemia (MI), and its mechanism is associated with the suppression of the Cytc- and caspase-signaling pathways. Trans-nerolol has a therapeutic effect on MI, and its mechanism of action is related to its anti-apoptotic effect. These results suggest thatDalbergia odoriferahas a potential role to be developed as an MI-promoting therapeutic agent.
研究领域:
药理学
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心血管疾病
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分子生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）